LOCUS       RG216778                9449 bp    DNA     circular  SYN 
DEFINITION  KIF5B (GFP-tagged) - Human kinesin family member 5B (KIF5B)
ACCESSION   NM_004521
VERSION     NM_004521.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3930..4634 
                     /label="GFP"  
     misc_feature    4681..5266 
                     /label="PolyA signal"  
     misc_feature    7358..8003 
                     /label="ColE1"  
     misc_feature    5720..6514 
                     /label="Neo"  
     misc_feature    5351..5653 
                     /label="SV40 ori"  
     misc_feature    8018..8878 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3909..3915 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3909 
                     /label="KIF5B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGACCTG GCCGAGTGCA ACATCAAAGT GATGTGTCGC TTCAGACCTC 
     1081 TCAACGAGTC TGAAGTGAAC CGCGGCGACA AGTACATCGC CAAGTTTCAG GGAGAAGACA 
     1141 CGGTCGTGAT CGCGTCCAAG CCTTATGCAT TTGATCGGGT GTTCCAGTCA AGCACATCTC 
     1201 AAGAGCAAGT GTATAATGAC TGTGCAAAGA AGATTGTTAA AGATGTACTT GAAGGATATA 
     1261 ATGGAACAAT ATTTGCATAT GGACAAACAT CCTCTGGGAA GACACACACA ATGGAGGGTA 
     1321 AACTTCATGA TCCAGAAGGC ATGGGAATTA TTCCAAGAAT AGTGCAAGAT ATTTTTAATT 
     1381 ATATTTACTC CATGGATGAA AATTTGGAAT TTCATATTAA GGTTTCATAT TTTGAAATAT 
     1441 ATTTGGATAA GATAAGGGAC CTGTTAGATG TTTCAAAGAC CAACCTTTCA GTTCATGAAG 
     1501 ACAAAAACCG AGTTCCCTAT GTAAAGGGGT GCACAGAGCG TTTTGTATGT AGTCCAGATG 
     1561 AAGTTATGGA TACCATAGAT GAAGGAAAAT CCAACAGACA TGTAGCAGTT ACAAATATGA 
     1621 ATGAACATAG CTCTAGGAGT CACAGTATAT TTCTTATTAA TGTCAAACAA GAGAACACAC 
     1681 AAACGGAACA AAAGCTGAGT GGAAAACTTT ATCTGGTTGA TTTAGCTGGT AGTGAAAAGG 
     1741 TTAGTAAAAC TGGAGCTGAA GGTGCTGTGC TGGATGAAGC TAAAAACATC AACAAGTCAC 
     1801 TTTCTGCTCT TGGAAATGTT ATTTCTGCTT TGGCTGAGGG TAGTACATAT GTTCCATATC 
     1861 GAGATAGTAA AATGACAAGA ATCCTTCAAG ATTCATTAGG TGGCAACTGT AGAACCACTA 
     1921 TTGTAATTTG CTGCTCTCCA TCATCATACA ATGAGTCTGA AACAAAATCT ACACTCTTAT 
     1981 TTGGCCAAAG GGCCAAAACA ATTAAGAACA CAGTTTGTGT CAATGTGGAG TTAACTGCAG 
     2041 AACAGTGGAA AAAGAAGTAT GAAAAAGAAA AAGAAAAAAA TAAGATCCTG CGGAACACTA 
     2101 TTCAGTGGCT TGAAAATGAG CTCAACAGAT GGCGTAATGG GGAGACGGTG CCTATTGATG 
     2161 AACAGTTTGA CAAAGAGAAA GCCAACTTGG AAGCTTTCAC AGTGGATAAA GATATTACTC 
     2221 TTACCAATGA TAAACCAGCA ACCGCAATTG GAGTTATAGG AAATTTTACT GATGCTGAAA 
     2281 GAAGAAAGTG TGAAGAAGAA ATTGCTAAAT TATACAAACA GCTTGATGAC AAGGATGAAG 
     2341 AAATTAACCA GCAAAGTCAA CTGGTAGAGA AACTGAAGAC GCAAATGTTG GATCAGGAGG 
     2401 AGCTTTTGGC ATCTACCAGA AGGGATCAAG ACAATATGCA AGCTGAGCTG AATCGCCTTC 
     2461 AAGCAGAAAA TGATGCCTCT AAAGAAGAAG TGAAAGAAGT TTTACAGGCC CTAGAAGAAC 
     2521 TTGCTGTCAA TTATGATCAG AAGTCTCAGG AAGTTGAAGA CAAAACTAAG GAATATGAAT 
     2581 TGCTTAGTGA TGAATTGAAT CAGAAATCGG CAACTTTAGC GAGTATAGAT GCTGAGCTTC 
     2641 AGAAACTTAA GGAAATGACC AACCACCAGA AAAAACGAGC AGCTGAGATG ATGGCATCTT 
     2701 TACTAAAAGA CCTTGCAGAA ATAGGAATTG CTGTGGGAAA TAATGATGTA AAGCAGCCTG 
     2761 AGGGAACTGG CATGATAGAT GAAGAGTTCA CTGTTGCAAG ACTCTACATT AGCAAAATGA 
     2821 AGTCAGAAGT AAAAACCATG GTGAAACGTT GCAAGCAGTT AGAAAGCACA CAAACTGAGA 
     2881 GCAACAAAAA AATGGAAGAA AATGAAAAGG AGTTAGCAGC ATGTCAGCTT CGTATCTCTC 
     2941 AACATGAAGC CAAAATCAAG TCATTGACTG AATACCTTCA AAATGTGGAA CAAAAGAAAA 
     3001 GACAGTTGGA GGAATCTGTC GATGCCCTCA GTGAAGAACT AGTCCAGCTT CGAGCACAAG 
     3061 AGAAAGTCCA TGAAATGGAA AAGGAGCACT TAAATAAGGT TCAGACTGCA AATGAAGTTA 
     3121 AGCAAGCTGT TGAACAGCAG ATCCAGAGCC ATAGAGAAAC TCATCAAAAA CAGATCAGTA 
     3181 GTTTGAGAGA TGAAGTAGAA GCAAAAGCAA AACTTATTAC TGATCTTCAA GACCAAAACC 
     3241 AGAAAATGAT GTTAGAGCAG GAACGTCTAA GAGTAGAACA TGAGAAGTTG AAAGCCACAG 
     3301 ATCAGGAAAA GAGCAGAAAA CTACATGAAC TTACGGTTAT GCAAGATAGA CGAGAACAAG 
     3361 CAAGACAAGA CTTGAAGGGT TTGGAAGAGA CAGTGGCAAA AGAACTTCAG ACTTTACACA 
     3421 ACCTGCGCAA ACTCTTTGTT CAGGACCTGG CTACAAGAGT TAAAAAGAGT GCTGAGATTG 
     3481 ATTCTGATGA CACCGGAGGC AGCGCTGCTC AGAAGCAAAA AATCTCCTTT CTTGAAAATA 
     3541 ATCTTGAACA GCTCACTAAA GTGCACAAAC AGTTGGTACG TGATAATGCA GATCTCCGCT 
     3601 GTGAACTTCC TAAGTTGGAA AAGCGACTTC GAGCTACAGC TGAGAGAGTG AAAGCTTTGG 
     3661 AATCAGCACT GAAAGAAGCT AAAGAAAATG CATCTCGTGA TCGCAAACGC TATCAGCAAG 
     3721 AAGTAGATCG CATAAAGGAA GCAGTCAGGT CAAAGAATAT GGCCAGAAGA GGGCATTCTG 
     3781 CACAGATTGC TAAACCTATT CGTCCCGGGC AACATCCAGC AGCTTCTCCA ACTCACCCAA 
     3841 GTGCAATTCG TGGAGGAGGT GCATTTGTTC AGAACAGCCA GCCAGTGGCA GTGCGAGGTG 
     3901 GAGGAGGCAA ACAAGTGACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3961 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     4021 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     4081 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     4141 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     4201 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4261 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4321 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4381 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4441 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4501 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4561 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4621 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4681 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4741 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4801 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4861 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4921 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4981 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5041 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5101 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5161 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5221 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5281 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5341 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5401 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5461 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5521 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5581 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5641 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5701 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5761 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5821 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5881 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5941 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     6001 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     6061 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     6121 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     6181 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6241 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6301 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6361 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6421 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6481 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6541 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6601 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6661 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6721 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6781 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6841 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6901 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6961 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     7021 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     7081 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     7141 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     7201 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7261 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7321 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7381 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7441 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7501 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7561 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7621 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7681 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7741 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7801 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7861 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7921 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7981 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     8041 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     8101 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     8161 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     8221 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8281 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8341 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8401 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8461 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8521 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8581 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8641 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8701 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8761 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8821 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8881 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8941 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     9001 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     9061 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     9121 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     9181 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9241 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9301 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9361 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9421 TGATTTAACA AAAATTTAAC GCGAATTTT
//