LOCUS       RG216727                9185 bp    DNA     circular  SYN 
DEFINITION  UBE3A (tGFP-tagged) - Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2
ACCESSION   NM_000462
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3666..4370 
                     /label="GFP"  
     misc_feature    4417..5002 
                     /label="PolyA signal"  
     misc_feature    7094..7739 
                     /label="ColE1"  
     misc_feature    5456..6250 
                     /label="Neo"  
     misc_feature    5087..5389 
                     /label="SV40 ori"  
     misc_feature    7754..8614 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3645..3651 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3645 
                     /label="UBE3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGCTG CACCAGTGTT ATTGGAAATC AGGAGAACCT CAGTCTGACG 
     1081 ACATTGAAGC TAGCCGAATG AAGCGAGCAG CTGCAAAGCA TCTAATAGAA CGCTACTACC 
     1141 ACCAGTTAAC TGAGGGCTGT GGAAATGAAG CCTGCACGAA TGAGTTTTGT GCTTCCTGTC 
     1201 CAACTTTTCT TCGTATGGAT AATAATGCAG CAGCTATTAA AGCCCTCGAG CTTTATAAGA 
     1261 TTAATGCAAA ACTCTGTGAT CCTCATCCCT CCAAGAAAGG AGCAAGCTCA GCTTACCTTG 
     1321 AGAACTCGAA AGGTGCCCCC AACAACTCCT GCTCTGAGAT AAAAATGAAC AAGAAAGGCG 
     1381 CTAGAATTGA TTTTAAAGAT GTGACTTACT TAACAGAAGA GAAGGTATAT GAAATTCTTG 
     1441 AATTATGTAG AGAAAGAGAG GATTATTCCC CTTTAATCCG TGTTATTGGA AGAGTTTTTT 
     1501 CTAGTGCTGA GGCATTGGTA CAGAGCTTCC GGAAAGTTAA ACAACACACC AAGGAAGAAC 
     1561 TGAAATCTCT TCAAGCAAAA GATGAAGACA AAGATGAAGA TGAAAAGGAA AAAGCTGCAT 
     1621 GTTCTGCTGC TGCTATGGAA GAAGACTCAG AAGCATCTTC CTCAAGGATA GGTGATAGCT 
     1681 CACAGGGAGA CAACAATTTG CAAAAATTAG GCCCTGATGA TGTGTCTGTG GATATTGATG 
     1741 CCATTAGAAG GGTCTACACC AGATTGCTCT CTAATGAAAA AATTGAAACT GCCTTTCTCA 
     1801 ATGCACTTGT ATATTTGTCA CCTAACGTGG AATGTGACTT GACGTATCAC AATGTATACT 
     1861 CTCGAGATCC TAATTATCTG AATTTGTTCA TTATCGTAAT GGAGAATAGA AATCTCCACA 
     1921 GTCCTGAATA TCTGGAAATG GCTTTGCCAT TATTTTGCAA AGCGATGAGC AAGCTACCCC 
     1981 TTGCAGCCCA AGGAAAACTG ATCAGACTGT GGTCTAAATA CAATGCAGAC CAGATTCGGA 
     2041 GAATGATGGA GACATTTCAG CAACTTATTA CTTATAAAGT CATAAGCAAT GAATTTAACA 
     2101 GTCGAAATCT AGTGAATGAT GATGATGCCA TTGTTGCTGC TTCGAAGTGC TTGAAAATGG 
     2161 TTTACTATGC AAATGTAGTG GGAGGGGAAG TGGACACAAA TCACAATGAA GAAGATGATG 
     2221 AAGAGCCCAT CCCTGAGTCC AGCGAGCTGA CACTTCAGGA ACTTTTGGGA GAAGAAAGAA 
     2281 GAAACAAGAA AGGTCCTCGA GTGGACCCCC TGGAAACTGA ACTTGGTGTT AAAACCCTGG 
     2341 ATTGTCGAAA ACCACTTATC CCTTTTGAAG AGTTTATTAA TGAACCACTG AATGAGGTTC 
     2401 TAGAAATGGA TAAAGATTAT ACTTTTTTCA AAGTAGAAAC AGAGAACAAA TTCTCTTTTA 
     2461 TGACATGTCC CTTTATATTG AATGCTGTCA CAAAGAATTT GGGATTATAT TATGACAATA 
     2521 GAATTCGCAT GTACAGTGAA CGAAGAATCA CTGTTCTCTA CAGCTTAGTT CAAGGACAGC 
     2581 AGTTGAATCC ATATTTGAGA CTCAAAGTTA GACGTGACCA TATCATAGAT GATGCACTTG 
     2641 TCCGGCTAGA GATGATCGCT ATGGAAAATC CTGCAGACTT GAAGAAGCAG TTGTATGTGG 
     2701 AATTTGAAGG AGAACAAGGA GTTGATGAGG GAGGTGTTTC CAAAGAATTT TTTCAGCTGG 
     2761 TTGTGGAGGA AATCTTCAAT CCAGATATTG GTATGTTCAC ATACGATGAA TCTACAAAAT 
     2821 TGTTTTGGTT TAATCCATCT TCTTTTGAAA CTGAGGGTCA GTTTACTCTG ATTGGCATAG 
     2881 TACTGGGTCT GGCTATTTAC AATAACTGTA TACTGGATGT ACATTTTCCC ATGGTTGTCT 
     2941 ACAGGAAGCT AATGGGGAAA AAAGGAACTT TTCGTGACTT GGGAGACTCT CACCCAGTTC 
     3001 TATATCAGAG TTTAAAAGAT TTATTGGAGT ATGAAGGGAA TGTGGAAGAT GACATGATGA 
     3061 TCACTTTCCA GATATCACAG ACAGATCTTT TTGGTAACCC AATGATGTAT GATCTAAAGG 
     3121 AAAATGGTGA TAAAATTCCA ATTACAAATG AAAACAGGAA GGAATTTGTC AATCTTTATT 
     3181 CTGACTACAT TCTCAATAAA TCAGTAGAAA AACAGTTCAA GGCTTTTCGG AGAGGTTTTC 
     3241 ATATGGTGAC CAATGAATCT CCCTTAAAGT ACTTATTCAG ACCAGAAGAA ATTGAATTGC 
     3301 TTATATGTGG AAGCCGGAAT CTAGATTTCC AAGCACTAGA AGAAACTACA GAATATGACG 
     3361 GTGGCTATAC CAGGGACTCT GTTCTGATTA GGGAGTTCTG GGAAATCGTT CATTCATTTA 
     3421 CAGATGAACA GAAAAGACTC TTCTTGCAGT TTACAACGGG CACAGACAGA GCACCTGTGG 
     3481 GAGGACTAGG AAAATTAAAG ATGATTATAG CCAAAAATGG CCCAGACACA GAAAGGTTAC 
     3541 CTACATCTCA TACTTGCTTT AATGTGCTTT TACTTCCGGA ATACTCAAGC AAAGAAAAAC 
     3601 TTAAAGAGAG ATTGTTGAAG GCCATCACGT ATGCCAAAGG ATTTGGCATG CTGACGCGTA 
     3661 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3721 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3781 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3841 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     3901 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     3961 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4021 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4081 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4141 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4201 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4261 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4321 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4381 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4441 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4501 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4561 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4621 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4681 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4741 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4801 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4861 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4921 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4981 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5041 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5101 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5161 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5221 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5281 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5341 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5401 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5461 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5521 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5581 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5641 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5701 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5761 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5821 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     5881 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     5941 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6001 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6061 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6121 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6181 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6241 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6301 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6361 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6421 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6481 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6541 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6601 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6661 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6721 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6781 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6841 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     6901 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     6961 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7021 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7081 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7141 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7201 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7261 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7321 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7381 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7441 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7501 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7561 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7621 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7681 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7741 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7801 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7861 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     7921 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     7981 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8041 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8101 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8161 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8221 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8281 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8341 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8401 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8461 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8521 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8581 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8641 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8701 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8761 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8821 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     8881 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     8941 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9001 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9061 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9121 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9181 ATTTT
//