LOCUS       RG216521                9305 bp    DNA     circular  SYN 
DEFINITION  ENPP2 (tGFP-tagged) - Human ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2), transcript variant 1
ACCESSION   NM_006209
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3786..4490 
                     /label="GFP"  
     misc_feature    4537..5122 
                     /label="PolyA signal"  
     misc_feature    7214..7859 
                     /label="ColE1"  
     misc_feature    5576..6370 
                     /label="Neo"  
     misc_feature    5207..5509 
                     /label="SV40 ori"  
     misc_feature    7874..8734 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3765..3771 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3765 
                     /label="ENPP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAGGAGG AGCTCGTTCC AGTCGTGTCA GATAATATCC CTGTTCACTT 
     1081 TTGCCGTTGG AGTCAGTATC TGCTTAGGAT TCACTGCACA TCGAATTAAG AGAGCAGAAG 
     1141 GATGGGAGGA AGGTCCTCCT ACAGTGCTAT CAGACTCCCC CTGGACCAAC ATCTCCGGAT 
     1201 CTTGCAAGGG CAGGTGCTTT GAACTTCAAG AGGCTGGACC TCCTGATTGT CGCTGTGACA 
     1261 ACTTGTGTAA GAGCTATACC AGTTGCTGCC ATGACTTTGA TGAGCTGTGT TTGAAGACAG 
     1321 CCCGTGGCTG GGAGTGTACT AAGGACAGAT GTGGAGAAGT CAGAAATGAA GAAAATGCCT 
     1381 GTCACTGCTC AGAGGACTGC TTGGCCAGGG GAGACTGCTG TACCAATTAC CAAGTGGTTT 
     1441 GCAAAGGAGA GTCGCATTGG GTTGATGATG ACTGTGAGGA AATAAAGGCC GCAGAATGCC 
     1501 CTGCAGGGTT TGTTCGCCCT CCATTAATCA TCTTCTCCGT GGATGGCTTC CGTGCATCAT 
     1561 ACATGAAGAA AGGCAGCAAA GTCATGCCTA ATATTGAAAA ACTAAGGTCT TGTGGCACAC 
     1621 ACTCTCCCTA CATGAGGCCG GTGTACCCAA CTAAAACCTT TCCTAACTTA TACACTTTGG 
     1681 CCACTGGGCT ATATCCAGAA TCACATGGAA TTGTTGGCAA TTCAATGTAT GATCCTGTAT 
     1741 TTGATGCCAC TTTTCATCTG CGAGGGCGAG AGAAATTTAA TCATAGATGG TGGGGAGGTC 
     1801 AACCGCTATG GATTACAGCC ACCAAGCAAG GGGTGAAAGC TGGAACATTC TTTTGGTCTG 
     1861 TTGTCATCCC TCACGAGCGG AGAATATTAA CCATATTGCG GTGGCTCACC CTGCCAGATC 
     1921 ATGAGAGGCC TTCGGTCTAT GCCTTCTATT CTGAGCAACC TGATTTCTCT GGACACAAAT 
     1981 ATGGCCCTTT CGGCCCTGAG GAGAGTAGTT ATGGCTCACC TTTTACTCCG GCTAAGAGAC 
     2041 CTAAGAGGAA AGTTGCCCCT AAGAGGAGAC AGGAAAGACC AGTTGCTCCT CCAAAGAAAA 
     2101 GAAGAAGAAA AATACATAGG ATGGATCATT ATGCTGCGGA AACTCGTCAG GACAAAATGA 
     2161 CAAATCCTCT GAGGGAAATC GACAAAATTG TGGGGCAATT AATGGATGGA CTGAAACAAC 
     2221 TAAAACTGCG TCGGTGTGTC AACGTCATCT TTGTCGGAGA CCATGGAATG GAAGATGTCA 
     2281 CATGTGATAG AACTGAGTTC TTGAGTAATT ACCTAACTAA TGTGGATGAT ATTACTTTAG 
     2341 TGCCTGGAAC TCTAGGAAGA ATTCGATCCA AATTTAGCAA CAATGCTAAA TATGACCCCA 
     2401 AAGCCATTAT TGCCAATCTC ACGTGTAAAA AACCAGATCA GCACTTTAAG CCTTACTTGA 
     2461 AACAGCACCT TCCCAAACGT TTGCACTATG CCAACAACAG AAGAATTGAG GATATCCATT 
     2521 TATTGGTGGA ACGCAGATGG CATGTTGCAA GGAAACCTTT GGATGTTTAT AAGAAACCAT 
     2581 CAGGAAAATG CTTTTTCCAG GGAGACCACG GATTTGATAA CAAGGTCAAC AGCATGCAGA 
     2641 CTGTTTTTGT AGGTTATGGC CCAACATTTA AGTACAAGAC TAAAGTGCCT CCATTTGAAA 
     2701 ACATTGAACT TTACAATGTT ATGTGTGATC TCCTGGGATT GAAGCCAGCT CCTAATAATG 
     2761 GGACCCATGG AAGTTTGAAT CATCTCCTGC GCACTAATAC CTTCAGGCCA ACCATGCCAG 
     2821 AGGAAGTTAC CAGACCCAAT TATCCAGGGA TTATGTACCT TCAGTCTGAT TTTGACCTGG 
     2881 GCTGCACTTG TGATGATAAG GTAGAGCCAA AGAACAAGTT GGATGAACTC AACAAACGGC 
     2941 TTCATACAAA AGGGTCTACA GAAGAGAGAC ACCTCCTCTA TGGGCGACCT GCAGTGCTTT 
     3001 ATCGGACTAG ATATGATATC TTATATCACA CTGACTTTGA AAGTGGTTAT AGTGAAATAT 
     3061 TCCTAATGCT ACTCTGGACA TCATATACTG TTTCCAAACA GGCTGAGGTT TCCAGCGTTC 
     3121 CTGACCATCT GACCAGTTGC GTCCGGCCTG ATGTCCGTGT TTCTCCGAGT TTCAGTCAGA 
     3181 ACTGTTTGGC CTACAAAAAT GATAAGCAGA TGTCCTACGG ATTCCTCTTT CCTCCTTATC 
     3241 TGAGCTCTTC ACCAGAGGCT AAATATGATG CATTCCTTGT AACCAATATG GTTCCAATGT 
     3301 ATCCTGCTTT CAAACGGGTC TGGAATTATT TCCAAAGGGT ATTGGTGAAG AAATATGCTT 
     3361 CGGAAAGAAA TGGAGTTAAC GTGATAAGTG GACCAATCTT CGACTATGAC TATGATGGCT 
     3421 TACATGACAC AGAAGACAAA ATAAAACAGT ACGTGGAAGG CAGTTCCATT CCTGTTCCAA 
     3481 CTCACTACTA CAGCATCATC ACCAGCTGTC TGGATTTCAC TCAGCCTGCC GACAAGTGTG 
     3541 ACGGCCCTCT CTCTGTGTCC TCCTTCATCC TGCCTCACCG GCCTGACAAC GAGGAGAGCT 
     3601 GCAATAGCTC AGAGGACGAA TCAAAATGGG TAGAAGAACT CATGAAGATG CACACAGCTA 
     3661 GGGTGCGTGA CATTGAACAT CTCACCAGCC TGGACTTCTT CCGAAAGACC AGCCGCAGCT 
     3721 ACCCAGAAAT CCTGACACTC AAGACATACC TGCATACATA TGAGAGCGAG ATTACGCGTA 
     3781 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3841 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3901 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3961 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     4021 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4081 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4141 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4201 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4261 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4321 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4381 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4441 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5221 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5281 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5341 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5401 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5461 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5521 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5581 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5641 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5701 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5761 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5821 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5881 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5941 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     6001 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6061 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6121 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6181 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6241 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6301 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6361 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6421 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6481 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6541 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6601 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6661 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6721 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6781 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6841 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6901 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6961 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7021 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7081 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7141 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7201 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7261 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7321 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7381 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7441 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7501 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7561 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7621 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7681 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7741 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7801 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7861 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7921 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7981 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     8041 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8101 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8161 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8221 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8281 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8341 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8401 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8461 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8521 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8581 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8641 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8701 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8761 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8821 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8881 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8941 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     9001 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9061 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9121 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9181 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9241 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9301 ATTTT
//