LOCUS       RG216227                9428 bp    DNA     circular  SYN 
DEFINITION  MATN2 (tGFP-tagged) - Human matrilin 2 (MATN2), transcript variant 1
ACCESSION   NM_002380
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3909..4613 
                     /label="GFP"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    7337..7982 
                     /label="ColE1"  
     misc_feature    5699..6493 
                     /label="Neo"  
     misc_feature    5330..5632 
                     /label="SV40 ori"  
     misc_feature    7997..8857 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3888..3894 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3888 
                     /label="MATN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAAGATG CTCGCAGGCT GCTTTCTGCT GATCCTCGGA CAGATCGTCC 
     1081 TCCTCCCTGC CGAGGCCAGG GAGCGGTCAC GTGGGAGGTC CATCTCTAGG GGCAGACACG 
     1141 CTCGGACCCA CCCGCAGACG GCCCTTCTGG AGAGTTCCTG TGAGAACAAG CGGGCAGACC 
     1201 TGGTTTTCAT CATTGACAGC TCTCGCAGTG TCAACACCCA TGACTATGCA AAGGTCAAGG 
     1261 AGTTCATCGT GGACATCTTG CAATTCTTGG ACATTGGTCC TGATGTCACC CGAGTGGGCC 
     1321 TGCTCCAATA TGGCAGCACT GTCAAGAATG AGTTCTCCCT CAAGACCTTC AAGAGGAAGT 
     1381 CCGAGGTGGA GCGTGCTGTC AAGAGGATGC GGCATCTGTC CACGGGCACC ATGACTGGGC 
     1441 TGGCCATCCA GTATGCCCTG AACATCGCAT TCTCAGAAGC AGAGGGGGCC CGGCCCCTGA 
     1501 GGGAGAATGT GCCACGGGTC ATAATGATCG TGACAGATGG GAGACCTCAG GACTCCGTGG 
     1561 CCGAGGTGGC TGCTAAGGCA CGGGACACGG GCATCCTAAT CTTTGCCATT GGTGTGGGCC 
     1621 AGGTAGACTT CAACACCTTG AAGTCCATTG GGAGTGAGCC CCATGAGGAC CATGTCTTCC 
     1681 TTGTGGCCAA TTTCAGCCAG ATTGAGACGC TGACCTCCGT GTTCCAGAAG AAGTTGTGCA 
     1741 CGGCCCACAT GTGCAGCACC CTGGAGCATA ACTGTGCCCA CTTCTGCATC AACATCCCTG 
     1801 GCTCATACGT CTGCAGGTGC AAACAAGGCT ACATTCTCAA CTCGGATCAG ACGACTTGCA 
     1861 GAATCCAGGA TCTGTGTGCC ATGGAGGACC ACAACTGTGA GCAGCTCTGT GTGAATGTGC 
     1921 CGGGCTCCTT CGTCTGCCAG TGCTACAGTG GCTACGCCCT GGCTGAGGAT GGGAAGAGGT 
     1981 GTGTGGCTGT GGACTACTGT GCCTCAGAAA ACCACGGATG TGAACATGAG TGTGTAAATG 
     2041 CTGATGGCTC CTACCTTTGC CAGTGCCATG AAGGATTTGC TCTTAACCCA GATAAAAAAA 
     2101 CGTGCACAAA GATAGACTAC TGTGCCTCAT CTAATCACGG ATGTCAGCAC GAGTGTGTTA 
     2161 ACACAGATGA TTCCTATTCC TGCCACTGCC TGAAAGGCTT TACCCTGAAT CCAGATAAGA 
     2221 AAACCTGCAG AAGGATCAAC TACTGTGCAC TGAACAAACC GGGCTGTGAG CATGAGTGCG 
     2281 TCAACATGGA GGAGAGCTAC TACTGCCGCT GCCACCGTGG CTACACTCTG GACCCCAATG 
     2341 GCAAAACCTG CAGCCGAGTG GACCACTGTG CACAGCAGGA CCATGGCTGT GAGCAGCTGT 
     2401 GTCTGAACAC GGAGGATTCC TTCGTCTGCC AGTGCTCAGA AGGCTTCCTC ATCAACGAGG 
     2461 ACCTCAAGAC CTGCTCCCGG GTGGATTACT GCCTGCTGAG TGACCATGGT TGTGAATACT 
     2521 CCTGTGTCAA CATGGACAGA TCCTTTGCCT GTCAGTGTCC TGAGGGACAC GTGCTCCGCA 
     2581 GCGATGGGAA GACGTGTGCA AAATTGGACT CTTGTGCTCT GGGGGACCAC GGTTGTGAAC 
     2641 ATTCGTGTGT AAGCAGTGAA GATTCGTTTG TGTGCCAGTG CTTTGAAGGT TATATACTCC 
     2701 GTGAAGATGG AAAAACCTGC AGAAGGAAAG ATGTCTGCCA AGCTATAGAC CATGGCTGTG 
     2761 AACACATTTG TGTGAACAGT GATGACTCAT ACACGTGCGA GTGCTTGGAG GGATTCCGGC 
     2821 TCGCTGAGGA TGGGAAACGC TGCCGAAGGA AGGATGTCTG CAAATCAACC CACCATGGCT 
     2881 GCGAACACAT TTGTGTTAAT AATGGGAATT CCTACATCTG CAAATGCTCA GAGGGATTTG 
     2941 TTCTAGCTGA GGACGGAAGA CGGTGCAAGA AATGCACTGA AGGCCCAATT GACCTGGTCT 
     3001 TTGTGATCGA TGGATCCAAG AGTCTTGGAG AAGAGAATTT TGAGGTCGTG AAGCAGTTTG 
     3061 TCACTGGAAT TATAGATTCC TTGACAATTT CCCCCAAAGC CGCTCGAGTG GGGCTGCTCC 
     3121 AGTATTCCAC ACAGGTCCAC ACAGAGTTCA CTCTGAGAAA CTTCAACTCA GCCAAAGACA 
     3181 TGAAAAAAGC CGTGGCCCAC ATGAAATACA TGGGAAAGGG CTCTATGACT GGGCTGGCCC 
     3241 TGAAACACAT GTTTGAGAGA AGTTTTACCC AAGGAGAAGG GGCCAGGCCC CTTTCCACAA 
     3301 GGGTGCCCAG AGCAGCCATT GTGTTCACCG ACGGACGGGC TCAGGATGAC GTCTCCGAGT 
     3361 GGGCCAGTAA AGCCAAGGCC AATGGTATCA CTATGTATGC TGTTGGGGTA GGAAAAGCCA 
     3421 TTGAGGAGGA ACTACAAGAG ATTGCCTCTG AGCCCACAAA CAAGCATCTC TTCTATGCCG 
     3481 AAGACTTCAG CACAATGGAT GAGATAAGTG AAAAACTCAA GAAAGGCATC TGTGAAGCTC 
     3541 TAGAAGACTC CGATGGAAGA CAGGACTCTC CAGCAGGGGA ACTGCCAAAA ACGGTCCAAC 
     3601 AGCCAACAGA ATCTGAGCCA GTCACCATAA ATATCCAAGA CCTACTTTCC TGTTCTAATT 
     3661 TTGCAGTGCA ACACAGATAT CTGTTTGAAG AAGACAATCT TTTACGGTCT ACACAAAAGC 
     3721 TTTCCCATTC AACAAAACCT TCAGGAAGCC CTTTGGAAGA AAAACACGAT CAATGCAAAT 
     3781 GTGAAAACCT TATAATGTTC CAGAACCTTG CAAACGAAGA AGTAAGAAAA TTAACACAGC 
     3841 GCTTAGAAGA AATGACACAG AGAATGGAAG CCCTGGAAAA TCGCCTGAGA TACAGAACGC 
     3901 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3961 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4021 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4081 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4141 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4201 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4261 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4321 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4381 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4441 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4501 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4561 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5341 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5401 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5461 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5521 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5581 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5641 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5701 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5761 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5821 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5881 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5941 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6001 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6061 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6121 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6181 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6241 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6301 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6361 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6421 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6481 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6541 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7021 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7081 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7141 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7201 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7261 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7321 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7381 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7441 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7501 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7561 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7621 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7681 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7741 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9001 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9061 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9121 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9181 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9241 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9301 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9361 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9421 CGAATTTT
//