LOCUS       RG215724                9431 bp    DNA     circular  SYN 
DEFINITION  COL21A1 (tGFP-tagged) - Human collagen, type XXI, alpha 1 (COL21A1)
ACCESSION   NM_030820
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3912..4616 
                     /label="GFP"  
     misc_feature    4663..5248 
                     /label="PolyA signal"  
     misc_feature    7340..7985 
                     /label="ColE1"  
     misc_feature    5702..6496 
                     /label="Neo"  
     misc_feature    5333..5635 
                     /label="SV40 ori"  
     misc_feature    8000..8860 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3891..3897 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3891 
                     /label="COL21A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCACTAT ATTACATTTC TCTGCATGGT TTTGGTGCTG CTTCTTCAGA 
     1081 ATTCTGTGTT AGCTGAAGAT GGGGAAGTAA GATCAAGTTG TCGTACTGCT CCGACAGATT 
     1141 TAGTTTTCAT CTTAGATGGC TCTTATAGTG TTGGCCCAGA AAACTTTGAA ATAGTGAAAA 
     1201 AGTGGCTTGT CAATATCACA AAAAACTTTG ACATAGGGCC GAAGTTTATT CAAGTTGGAG 
     1261 TGGTTCAATA TAGTGACTAC CCTGTGCTGG AGATTCCTCT CGGAAGCTAT GATTCAGGAG 
     1321 AACATTTGAC GGCAGCAGTG GAATCCATAC TCTACTTAGG AGGAAACACA AAGACAGGGA 
     1381 AGGCCATCCA GTTTGCGCTC GATTACCTTT TTGCCAAGTC CTCACGATTT CTGACTAAGA 
     1441 TAGCAGTGGT ACTTACGGAT GGCAAGTCCC AAGATGACGT CAAGGATGCA GCTCAAGCAG 
     1501 CAAGAGATAG TAAGATAACA TTATTTGCTA TTGGTGTTGG TTCAGAAACA GAAGATGCCG 
     1561 AACTTAGAGC TATTGCCAAC AAGCCTTCGT CTACTTATGT GTTTTATGTG GAAGACTATA 
     1621 TTGCAATATC CAAAATAAGG GAAGTGATGA AGCAGAAACT TTGTGAAGAA TCTGTCTGTC 
     1681 CAACACGAAT TCCAGTGGCA GCTCGTGATG AAAGGGGATT TGATATTCTT TTGGGTTTAG 
     1741 ATGTAAATAA AAAGGTTAAG AAAAGAATAC AGCTTTCACC AAAAAAGATA AAAGGATATG 
     1801 AAGTAACATC AAAAGTTGAT TTATCAGAAC TCACAAGCAA TGTTTTCCCA GAAGGTCTTC 
     1861 CTCCATCATA TGTATTTGTG TCTACTCAAA GATTTAAAGT CAAGAAAATT TGGGATTTAT 
     1921 GGAGAATATT AACTATTGAT GGAAGGCCAC AAATAGCAGT TACCTTAAAT GGTGTGGACA 
     1981 AAATCTTATT ATTTACAACA ACCAGCGTAA TTAATGGCTC ACAAGTGGTT ACCTTTGCTA 
     2041 ACCCTCAAGT TAAGACGTTG TTTGATGAAG GCTGGCACCA AATTCGTCTC TTAGTAACAG 
     2101 AACAAGATGT GACTTTGTAT ATTGATGACC AACAAATTGA AAACAAGCCC TTACATCCAG 
     2161 TTTTAGGGAT CTTGATCAAT GGGCAAACCC AAATTGGAAA ATATTCTGGA AAAGAAGAAA 
     2221 CTGTTCAGTT TGATGTCCAA AAGTTGCGAA TCTACTGTGA CCCAGAACAG AACAACCGGG 
     2281 AGACAGCATG TGAGATTCCT GGATTTAATG GAGAGTGCCT TAATGGTCCC AGTGATGTAG 
     2341 GTTCAACTCC AGCTCCCTGT ATTTGTCCTC CGGGAAAACC AGGACTTCAA GGCCCCAAAG 
     2401 GTGACCCTGG ACTGCCTGGG AACCCTGGCT ACCCTGGACA ACCTGGTCAA GATGGTAAGC 
     2461 CTGGATATCA GGGAATTGCA GGGACACCAG GTGTTCCAGG ATCTCCAGGA ATACAAGGAG 
     2521 CTCGAGGACT ACCAGGTTAC AAAGGAGAAC CAGGGCGAGA TGGTGACAAG GGTGATCGTG 
     2581 GACTTCCTGG TTTTCCTGGG CTTCATGGCA TGCCAGGATC AAAGGGTGAA ATGGGTGCCA 
     2641 AAGGAGACAA AGGATCACCT GGATTTTATG GCAAAAAGGG TGCAAAAGGT GAAAAGGGGA 
     2701 ATGCTGGCTT CCCTGGCCTC CCTGGACCTG CTGGAGAACC AGGAAGACAT GGAAAGGATG 
     2761 GATTAATGGG TAGTCCCGGT TTCAAGGGAG AAGCAGGATC CCCTGGTGCT CCGGGGCAGG 
     2821 ATGGAACACG GGGAGAGCCT GGAATCCCAG GATTTCCTGG AAACCGAGGA TTAATGGGCC 
     2881 AAAAGGGAGA AATTGGGCCT CCAGGACAGC AAGGAAAAAA AGGAGCCCCA GGGATGCCTG 
     2941 GTTTAATGGG AAGCAATGGC TCACCAGGCC AGCCTGGAAC ACCGGGATCT AAGGGAAGCA 
     3001 AAGGTGAACC TGGAATTCAA GGGATGCCTG GGGCTTCAGG GCTCAAGGGA GAACCAGGAG 
     3061 CAACGGGTTC CCCAGGAGAA CCAGGATACA TGGGTTTACC CGGGATTCAA GGAAAAAAGG 
     3121 GGGACAAAGG AAATCAAGGT GAAAAAGGTA TTCAGGGTCA AAAGGGAGAA AATGGAAGAC 
     3181 AGGGAATTCC AGGGCAACAG GGAATTCAAG GCCATCATGG TGCAAAAGGA GAGAGAGGTG 
     3241 AAAAGGGAGA ACCTGGTGTC CGAGGTGCCA TTGGATCAAA AGGAGAATCT GGGGTGGATG 
     3301 GCTTGATGGG GCCCGCAGGT CCTAAGGGGC AACCTGGGGA TCCAGGTCCT CAGGGACCCC 
     3361 CAGGTTTGGA TGGGAAGCCC GGAAGAGAGT TTTCAGAACA ATTTATTCGA CAAGTTTGCA 
     3421 CAGATGTAAT AAGAGCCCAG CTACCAGTCT TACTTCAGAG TGGAAGAATT AGAAATTGTG 
     3481 ATCATTGCCT GTCCCAACAT GGCTCCCCGG GTATTCCTGG GCCACCTGGT CCGATAGGCC 
     3541 CAGAGGGTCC CAGAGGATTA CCTGGTTTGC CAGGAAGAGA TGGTGTTCCT GGATTAGTGG 
     3601 GTGTCCCTGG ACGTCCAGGT GTCAGAGGAT TAAAAGGCCT ACCAGGAAGA AATGGGGAAA 
     3661 AAGGGAGCCA AGGGTTTGGG TATCCTGGAG AACAAGGTCC TCCTGGTCCC CCAGGTCCAG 
     3721 AGGGCCCTCC TGGAATAAGC AAAGAAGGTC CTCCAGGAGA CCCAGGTCTC CCTGGCAAAG 
     3781 ATGGAGACCA TGGAAAACCT GGAATCCAAG GGCAACCAGG CCCCCCAGGC ATCTGCGACC 
     3841 CATCACTATG TTTTAGTGTA ATTGCCAGAA GAGATCCGTT CAGAAAAGGA CCAAACTATA 
     3901 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     3961 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     4021 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     4081 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     4141 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     4201 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     4261 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     4321 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     4381 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     4441 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     4501 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4561 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     5341 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5401 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5461 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     5521 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5581 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5641 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5701 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5761 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5821 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5881 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     5941 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     6001 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     6061 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     6121 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     6181 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     6241 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     6301 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     6361 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     6421 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     6481 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6541 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     6961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     7021 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     7081 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     7141 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     7201 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     7261 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     7321 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     7381 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     7441 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     7501 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7561 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7621 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7681 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7741 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7801 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7861 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7921 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     7981 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     8041 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     8101 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     8161 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     8221 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     8281 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     8341 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     8401 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     8461 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     8521 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8581 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8641 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8701 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8761 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8821 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8881 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     8941 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     9001 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     9061 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     9121 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     9181 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     9241 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     9301 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     9361 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     9421 ACGCGAATTT T
//