LOCUS       RG214706                9608 bp    DNA     circular  SYN 
DEFINITION  CNTN1 (tGFP-tagged) - Human contactin 1 (CNTN1), transcript variant 1
ACCESSION   NM_001843
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4089..4793 
                     /label="GFP"  
     misc_feature    4840..5425 
                     /label="PolyA signal"  
     misc_feature    7517..8162 
                     /label="ColE1"  
     misc_feature    5879..6673 
                     /label="Neo"  
     misc_feature    5510..5812 
                     /label="SV40 ori"  
     misc_feature    8177..9037 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4068..4074 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4068 
                     /label="CNTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGTTGCTG GTCAGTCATC TTGTGATAAT ATCTATTACT ACCTGTTTAG 
     1081 CAGAGTTTAC ATGGTATAGA AGATATGGTC ATGGAGTTTC TGAGGAAGAC AAAGGATTTG 
     1141 GACCAATTTT TGAAGAGCAG CCAATCAATA CCATTTATCC AGAGGAATCA CTGGAAGGAA 
     1201 AAGTCTCACT CAACTGTAGG GCACGAGCCA GCCCTTTCCC GGTTTACAAA TGGAGAATGA 
     1261 ATAATGGGGA CGTTGATCTC ACAAGTGATC GATACAGTAT GGTAGGAGGA AACCTTGTTA 
     1321 TCAACAACCC TGACAAACAG AAAGATGCTG GAATATACTA CTGTTTAGCA TCTAATAACT 
     1381 ACGGGATGGT CAGAAGCACT GAAGCAACCC TGAGCTTTGG ATATCTTGAT CCTTTCCCAC 
     1441 CTGAGGAACG TCCTGAGGTC AGAGTAAAAG AAGGGAAAGG AATGGTGCTT CTCTGTGACC 
     1501 CCCCATACCA TTTTCCAGAT GATCTTAGCT ATCGCTGGCT TCTAAATGAA TTTCCTGTAT 
     1561 TTATCACAAT GGATAAACGG CGATTTGTGT CTCAGACAAA TGGCAATCTC TACATTGCAA 
     1621 ATGTTGAGGC TTCCGACAAA GGCAATTATT CCTGCTTTGT TTCCAGTCCT TCTATTACAA 
     1681 AGAGCGTGTT CAGCAAATTC ATCCCACTCA TTCCAATACC TGAACGAACA ACAAAACCAT 
     1741 ATCCTGCTGA TATTGTAGTT CAGTTCAAGG ATGTATATGC ATTGATGGGC CAAAATGTGA 
     1801 CCTTAGAATG TTTTGCACTT GGAAATCCTG TTCCGGATAT CCGATGGCGG AAGGTTCTAG 
     1861 AACCAATGCC AAGCACTGCT GAGATTAGCA CCTCTGGGGC TGTTCTTAAG ATCTTCAATA 
     1921 TTCAGCTAGA AGATGAAGGC ATCTATGAAT GTGAGGCTGA GAACATTAGA GGAAAGGATA 
     1981 AACATCAAGC AAGAATTTAT GTTCAAGCAT TCCCTGAGTG GGTAGAACAC ATCAATGACA 
     2041 CAGAGGTGGA CATAGGCAGT GATCTCTACT GGCCTTGTGT GGCCACAGGA AAGCCCATCC 
     2101 CTACAATCCG ATGGTTGAAA AATGGATATG CGTATCATAA AGGGGAATTA AGACTGTATG 
     2161 ATGTGACTTT TGAAAATGCC GGAATGTATC AGTGCATAGC TGAAAACACA TATGGAGCCA 
     2221 TTTATGCAAA TGCTGAGTTG AAGATCTTGG CGTTGGCTCC AACTTTTGAA ATGAATCCTA 
     2281 TGAAGAAAAA GATCCTGGCT GCTAAAGGTG GAAGGGTGAT AATTGAATGC AAACCTAAAG 
     2341 CTGCACCGAA ACCAAAGTTT TCATGGAGTA AAGGGACAGA GTGGCTTGTC AATAGCAGCA 
     2401 GAATACTCAT TTGGGAAGAT GGTAGCTTGG AAATCAATAA CATTACAAGG AATGATGGAG 
     2461 GTATCTATAC ATGCTTTGCA GAAAATAACA GAGGGAAAGC TAATAGCACT GGAACCCTTG 
     2521 TTATCACAGA TCCTACGCGA ATTATATTGG CCCCAATTAA TGCCGATATC ACAGTTGGAG 
     2581 AAAACGCCAC CATGCAGTGT GCTGCGTCCT TTGATCCTGC CTTGGATCTC ACATTTGTTT 
     2641 GGTCCTTCAA TGGCTATGTG ATCGATTTTA ACAAAGAGAA TATTCACTAC CAGAGGAATT 
     2701 TTATGCTGGA TTCCAATGGG GAATTACTAA TCCGAAATGC GCAGCTGAAA CATGCTGGAA 
     2761 GATACACATG CACTGCCCAG ACAATTGTGG ACAATTCTTC AGCTTCAGCT GACCTTGTAG 
     2821 TGAGAGGCCC TCCAGGCCCT CCAGGTGGTC TGAGAATAGA AGACATTAGA GCCACTTCTG 
     2881 TGGCACTTAC TTGGAGCCGT GGTTCAGACA ATCATAGTCC TATTTCTAAA TACACTATCC 
     2941 AGACCAAGAC TATTCTTTCA GATGACTGGA AAGATGCAAA GACAGATCCC CCAATTATTG 
     3001 AAGGAAATAT GGAGGCAGCA AGAGCAGTGG ACTTAATCCC ATGGATGGAG TATGAATTCC 
     3061 GCGTGGTAGC AACCAATACA CTGGGTAGAG GAGAGCCCAG TATACCATCT AACAGAATTA 
     3121 AAACAGACGG TGCTGCACCA AATGTGGCTC CTTCAGATGT AGGAGGTGGA GGTGGAAGAA 
     3181 ACAGAGAGCT GACCATAACA TGGGCGCCTT TGTCAAGAGA ATACCACTAT GGCAACAATT 
     3241 TTGGTTACAT AGTGGCATTT AAGCCATTTG ATGGAGAAGA ATGGAAAAAA GTCACAGTTA 
     3301 CTAATCCTGA TACTGGCCGA TATGTCCATA AAGATGAAAC CATGAGCCCT TCCACTGCAT 
     3361 TTCAAGTTAA AGTCAAGGCC TTCAACAACA AAGGAGATGG ACCTTACAGC CTAGTAGCAG 
     3421 TCATTAATTC AGCACAAGAC GCTCCCAGTG AAGCCCCAAC AGAAGTAGGT GTAAAAGTCT 
     3481 TATCATCTTC TGAGATATCT GTTCATTGGG AACATGTTTT AGAAAAAATA GTGGAAAGCT 
     3541 ATCAGATTCG GTATTGGGCT GCCCATGACA AAGAAGAAGC TGCAAACAGA GTTCAAGTCA 
     3601 CCAGCCAAGA GTACTCGGCC AGGCTCGAGA ACCTTCTGCC AGACACCCAG TATTTTATAG 
     3661 AAGTCGGGGC CTGCAATAGT GCAGGGTGTG GACCTCCAAG TGACATGATT GAGGCTTTCA 
     3721 CCAAGAAAGC ACCTCCTAGC CAGCCTCCAA GGATCATCAG TTCAGTAAGG TCTGGTTCAC 
     3781 GCTATATAAT CACCTGGGAT CATGTCGTTG CACTATCAAA TGAATCTACA GTGACGGGAT 
     3841 ATAAGGTACT CTACAGACCT GATGGCCAGC ATGATGGCAA GCTGTATTCA ACTCACAAAC 
     3901 ACTCCATAGA AGTCCCAATC CCCAGAGATG GAGAATACGT TGTGGAGGTT CGCGCGCACA 
     3961 GTGATGGAGG AGATGGAGTG GTGTCTCAAG TCAAAATTTC AGGTGCACCC ACCCTATCCC 
     4021 CAAGTCTTCT CGGCTTACTG CTGCCTGCCT TTGGCATCCT TGTCTACTTG GAATTCACGC 
     4081 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     4141 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4201 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4261 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4321 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4381 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4441 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4501 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4561 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4621 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4681 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4741 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4801 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4861 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4921 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4981 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5041 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5101 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5161 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5221 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5281 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5341 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5401 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5461 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5521 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5581 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5641 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5701 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5761 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5821 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5881 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5941 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     6001 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     6061 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     6121 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6181 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6241 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6301 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6361 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6421 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6481 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6541 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6601 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6661 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6721 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6781 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6841 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6901 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6961 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     7021 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     7081 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     7141 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7201 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7261 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7321 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7381 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7441 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7501 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7561 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7621 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7681 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7741 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7801 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7861 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7921 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7981 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     8041 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     8101 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     8161 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8221 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8281 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8341 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8401 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8461 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8521 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8581 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8641 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8701 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8761 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8821 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8881 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8941 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     9001 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     9061 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     9121 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9181 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9241 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9301 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9361 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9421 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9481 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9541 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9601 CGAATTTT
//