LOCUS       RG214319                9284 bp    DNA     circular  SYN 
DEFINITION  SKIDA1 (tGFP-tagged) - Human chromosome 10 open reading frame 140 (C10orf140). Note: ORF is codon optimized
ACCESSION   NM_207371
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3765..4469 
                     /label="GFP"  
     misc_feature    4516..5101 
                     /label="PolyA signal"  
     misc_feature    7193..7838 
                     /label="ColE1"  
     misc_feature    5555..6349 
                     /label="Neo"  
     misc_feature    5186..5488 
                     /label="SV40 ori"  
     misc_feature    7853..8713 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3744..3750 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3744 
                     /label="SKIDA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGATTTG AAAAGTGGCT TCGAGGAAGT CGATGGAGTG CGACTGGGAT 
     1081 ACCTGATTAT AAAGGGAAAA CAGATGTTCG CTCTCTCCCA GGTGTTCACA GATCTGCTGA 
     1141 AAAATATCCC TAGGACAACG GTCCACAAGC GAATGGACCA CTTGAAGGTC AAGAAACACC 
     1201 ACTGCGACCT CGAGGAACTG CGCAAACTGA AGGCAATTAA TAGCATCGCC TTTCACGCCG 
     1261 CTAAATGCAC CCTGATTAGC CGCGAGGACG TAGAAGCCCT CTACACGTCC TGTAAGACCG 
     1321 AGAGGGTCCT TAAAACGAAG CGACGCCGCG TCGGAAGGGC CTTGGCCACT AAGGCGCCTC 
     1381 CCCCTGAACG CGCCGCTGCA GCCAGCCCTC GACCCGGCTT CTGGAAGGAC AAACACCAGC 
     1441 TTTGGAGAGG TCTTTCAGGC GCCGCACGAC CCCTCCCCAT ATCCGCGCAG TCCCAGCGGC 
     1501 CAGGTGCCGC TGCTGCTCGG CCAGCCGCTC ACTTGCCCCA GATCTTTAGC AAATATCCAG 
     1561 GATCTCACTA CCCAGAGATC GTGCGGTCCC CGTGCAAGCC CCCACTTAAC TATGAGACGG 
     1621 CTCCTCTGCA AGGGAATTAC GTGGCCTTCC CCTCCGACCC CGCTTACTTT CGCAGCCTGC 
     1681 TGTGCTCAAA GCACCCTGCA GCCGCTGCCG CCGCTGCCGC AGCCGCCGCC GCCGCGGCCG 
     1741 CAGCAGCAGC TGCAGCTGCT TATTACCAGG TGTCAGCCGC CGGCCCTCAG CCCAAGGCTG 
     1801 CTGCTGGCGC AGGTGGCCCT GGAAGTCTGT CCTACAGATG TAAGCGAAAA CGGGGCGGAG 
     1861 CCAAGGATTG CCTTCTGGCT CCTCACGCGG GTGCGAGAAG ATTGCTCCTG CTGCCTCGCA 
     1921 GCTACAAGGC AAAGGCGGCG GCGGCCGCTG CCGCTGCGGC CGCCGCAGCC GCAGCCGCCG 
     1981 CTGGCGCCAC GTGTCTCGAG AGGTTTCATT TGGTCAATGG GTTTTGCCCG CCACCACACC 
     2041 ACCACCACCA TCACCATCAC CACCACCACC ACCACCATCA CAGAGCTCAG CCCCCCCAGC 
     2101 AGTCCCACCA TCCTCCTCAC CACCATCGGC CACAGCCACA TTTGGGTTCA TTTCCGGAGT 
     2161 CATGTTCCTC TGACAGCGAG TCTTCCTCAT ACAGTGACCA TGCAGCAAAT GACTCTGATT 
     2221 TTGGCAGCAG CCTGAGCAGC TCTAGCAACA GCGTGTCTTC CGAGGAAGAG GAGGAGGAGG 
     2281 GGGAAGAAGA GGAGGAGGAA GAGGAGGAGG AAGGCGGTTC AGGAGCCTCC GACTCTTCTG 
     2341 AAGTGTCTTC AGAGGAAGAG GACAGCTCTA CCGAAAGCGA CTCATCCAGT GGTTCTTCTC 
     2401 AGGTGAGTGT GCAGTCCATC AGGTTTAGAA GGACCTCTTT CTGTAAGCCG CCCAGCGTCC 
     2461 AAGCCCAGGC CAATTTCCTC TACCACCTGG CTTCCGCAGC CGCAGCGACC AAGCCTGCGG 
     2521 CCTTCGAAGA TGCAGGGAGA CTGCCTGATC TTAAATCCTC CGTGAAAGCT GAGAGCCCCG 
     2581 CAGAGTGGAA TCTTCAGTCT TGGGCCCCAA AGGCATCACC TGTTTATTGC CCTGCATCTC 
     2641 TGGGATCCTG CTTTGCTGAA ATCCGGAATG ACCGCGTCAG CGAAATCACA TTCCCCCATA 
     2701 GTGAAATATC TAACGCGGTG AAGCGGACAG ATCTCACTAT CAATTGTCTG GCAGAGGGCG 
     2761 CCTCTAGTCC CAGTCCTAAG ACCAACAACG CCTTCCCCCA GCAGAGGATA TTGAGAGAGG 
     2821 CTAGAAAATG TCTGCAAACT ACACCAACAA CCCACTGCGC CGACAACAAC ACCATTGCCG 
     2881 CTCGGTTCCT GAACAACGAC AGCTCCGGCG CGGAGGCAAA TTCCGAAAAG TACTCTAAAA 
     2941 TCCTGCACTG CCCTGAGTTC GCGACTGACC TGCCTTCATC CCAGACTGAC CCGGAGGTCA 
     3001 ATGCTGCCGG GGCTGCCGCC ACCAAGGCTG AAAACCCTTG TACCGATACA GGAGATAAGA 
     3061 CTCTCCCGTT TCTCCATAAC ATCAAGATCA AAGTGGAAGA CTCATCCGCG AATGAGGAGT 
     3121 ATGAACCGCA TTTGTTCACC AACAAGCTGA AGTGTGAGTG CAATGACACT AAAGGCGAAT 
     3181 TTTATTCTGT TACCGAGAGT AAGGAGGAGG ATGCCCTGCT GACCACAGCG AAGGAAGGAT 
     3241 TTGCTTGTCC TGAAAAAGAG ACACCTAGCC TGAACCCCCT GGCCCAGTCA CAAGGTTTGA 
     3301 GCTGCACGCT GGGCTCACCA AAACCCGAGG ATGGCGAATA CAAGTTCGGA GCTAGAGTGC 
     3361 GAAAAAACTA CAGAACACTG GTGCTCGGCA AGAGGCCGGT TTTGCAGACT CCTCCCGTCA 
     3421 AACCTAACCT CAAAAGTGCG CGAAGCCCAC GCCCCACTGG CAAAACTGAA ACTAACGAGG 
     3481 GGACACTGGA CGACTTCACT GTTATCAACA GGCGCAAAAA GGTCGCCAGC AATGTGGCAT 
     3541 CTGCTGTGAA GCGGCCCTTT CATTTTATGG CCAATTTCCC TTGCCCACCC AGTCTCATAA 
     3601 TCGGGAGGGA CGGTGATCTG TGGCCCGCTT ATTCACTGAA CACAACAAAA GACAGCCAGA 
     3661 CTCCACACAA GGCGCATCCT ATCTGGAAAT GGCAACTTGG AGGCTCAGCA ATCCCCCTGC 
     3721 CCCCTTCCCA TAAATTTAGA AAATTTAACT CAACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3781 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3841 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3901 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3961 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4021 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4081 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4141 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4201 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4261 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4321 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4381 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4441 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5161 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5221 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5281 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5341 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5401 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5461 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5521 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5581 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5641 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5701 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5761 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5821 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5881 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5941 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6001 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6061 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6121 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6181 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6241 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6301 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6361 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6421 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6481 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6541 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6601 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6661 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6721 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6781 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6841 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6901 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6961 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7021 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7081 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7141 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7201 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7261 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7321 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7381 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7441 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7501 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7561 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7621 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7681 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7741 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7801 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7861 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7921 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7981 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8041 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8101 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8161 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8221 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8281 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8341 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8401 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8461 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8521 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8581 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8641 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8701 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8761 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8821 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8881 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8941 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9001 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9061 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9121 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9181 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9241 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//