LOCUS       RG213683                7724 bp    DNA     circular  SYN 
DEFINITION  CADM4 (tGFP-tagged) - Human cell adhesion molecule 4 (CADM4)
ACCESSION   NM_145296
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2205..2909 
                     /label="GFP"  
     misc_feature    2956..3541 
                     /label="PolyA signal"  
     misc_feature    5633..6278 
                     /label="ColE1"  
     misc_feature    3995..4789 
                     /label="Neo"  
     misc_feature    3626..3928 
                     /label="SV40 ori"  
     misc_feature    6293..7153 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2184..2190 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2184 
                     /label="CADM4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCGGGCC CGGCGCTTCC AGTGGCCGCT GCTGCTGCTG TGGGCGGCCG 
     1081 CGGCGGGGCC AGGGGCAGGA CAGGAAGTAC AGACAGAGAA CGTGACAGTG GCTGAGGGTG 
     1141 GGGTGGCTGA GATCACCTGC CGTCTGCACC AGTATGATGG GTCCATAGTT GTCATCCAGA 
     1201 ACCCAGCCCG GCAGACCCTC TTCTTCAATG GCACCCGTGC CTTGAAGGAT GAGCGTTTCC 
     1261 AGCTTGAGGA GTTCTCCCCA CGCCGGGTGC GGATCCGGCT CTCAGATGCC CGCCTGGAGG 
     1321 ACGAGGGGGG CTATTTCTGC CAGCTCTACA CAGAAGACAC CCACCACCAG ATTGCCACGC 
     1381 TCACGGTACT AGTGGCCCCA GAGAATCCTG TGGTGGAGGT CCGGGAGCAG GCGGTAGAGG 
     1441 GCGGCGAGGT GGAGCTCAGC TGCCTCGTTC CGCGGTCCCG TCCGGCTGCC ACCCTGCGCT 
     1501 GGTACCGGGA CCGCAAGGAG CTGAAAGGAG TGAGCAGCAG CCAGGAAAAT GGCAAGGTCT 
     1561 GGAGCGTGGC AAGCACAGTA CGGTTTCGTG TGGACCGTAA GGACGACGGT GGTATCATCA 
     1621 TCTGTGAGGC GCAGAACCAG GCGCTGCCCT CCGGACACAG CAAGCAGACG CAGTACGTGC 
     1681 TGGATGTGCA GTACTCCCCC ACGGCCCGGA TTCATGCCTC CCAAGCTGTG GTGAGGGAGG 
     1741 GAGACACGCT GGTGTTGACG TGTGCTGTCA CGGGGAACCC CAGGCCAAAC CAGATCCGCT 
     1801 GGAACCGCGG GAATGAGTCT TTGCCGGAGA GGGCGGAGGC CGTGGGAGAG ACGCTCACGC 
     1861 TGCCGGGTCT GGTATCCGCG GATAACGGCA CCTACACTTG CGAGGCGTCC AATAAGCACG 
     1921 GCCATGCGAG GGCGCTCTAC GTACTTGTGG TCTACGACCC TGGTGCGGTG GTAGAGGCTC 
     1981 AGACGTCGGT TCCCTATGCC ATTGTGGGCG GCATCCTGGC GCTGCTGGTG TTTCTGATCA 
     2041 TATGTGTGCT AGTGGGCATG GTCTGGTGCT CGGTACGGCA GAAGGGTTCC TATCTGACCC 
     2101 ACGAAGCCAG TGGCTTGGAT GAACAGGGAG AAGCAAGAGA AGCCTTCCTC AATGGCAGCG 
     2161 ACGGACACAA GAGGAAAGAG GAATTCTTCA TCACGCGTAC GCGGCCGCTC GAGATGGAGA 
     2221 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     2281 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     2341 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     2401 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     2461 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     2521 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     2581 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     2641 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     2701 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     2761 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     2821 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     2881 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     2941 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3001 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3061 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3121 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3181 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3241 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3301 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3361 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3421 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3481 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3541 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     3601 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     3661 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     3721 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     3781 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     3841 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     3901 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     3961 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     4021 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     4081 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     4141 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     4201 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     4261 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     4321 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     4381 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     4441 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     4501 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     4561 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     4621 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     4681 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     4741 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     4801 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     4861 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     4921 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     4981 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     5041 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     5101 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     5161 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     5221 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     5281 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     5341 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     5401 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     5461 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     5521 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     5581 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     5641 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     5701 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     5761 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     5821 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     5881 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     5941 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     6001 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     6061 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     6121 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     6181 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     6241 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     6301 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     6361 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     6421 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     6481 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     6541 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     6601 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     6661 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     6721 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     6781 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     6841 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     6901 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     6961 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     7021 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     7081 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     7141 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     7201 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     7261 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     7321 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     7381 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     7441 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     7501 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     7561 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     7621 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     7681 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//