LOCUS       RG213399                9425 bp    DNA     circular  SYN 
DEFINITION  TJP3 (tGFP-tagged) - Human tight junction protein 3 (zona occludens 3) (TJP3)
ACCESSION   NM_014428
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3904..4608 
                     /label="GFP"  
     misc_feature    4655..5240 
                     /label="PolyA signal"  
     misc_feature    7332..7977 
                     /label="ColE1"  
     misc_feature    5694..6488 
                     /label="Neo"  
     misc_feature    5325..5627 
                     /label="SV40 ori"  
     misc_feature    7992..8852 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3876..3883 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3876 
                     /label="TJP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCTGTGT GGCCTCATGC CCATCTTCCC CGCTCCCCTC GACCAGGTGG 
     1081 CTGACATGGA GGAGCTGACC ATCTGGGAAC AGCACACGGC CACACTGTCC AAGGACCCCC 
     1141 GCCGGGGCTT TGGCATTGCG ATCTCTGGAG GCCGAGACCG GCCCGGTGGA TCCATGGTTG 
     1201 TATCTGACGT GGTACCTGGA GGGCCGGCGG AGGGCAGGCT ACAGACAGGC GACCACATCG 
     1261 TCATGGTGAA CGGGGTTTCC ATGGAGAATG CCACCTCCGC GTTTGCCATT CAGATACTCA 
     1321 AGACCTGCAC CAAGATGGCC AACATCACAG TGAAACGTCC CCGGAGGATC CACCTGCCCG 
     1381 CCACCAAAGC CAGCCCCTCC AGCCCAGGGC GCCAGGACTC GGATGAAGAC GATGGGCCCC 
     1441 AGCGGGTGGA GGAGGTGGAC CAGGGCCGGG GCTATGACGG CGACTCATCC AGTGGCTCCG 
     1501 GCCGCTCCTG GGACGAGCGC TCCCGCCGGC CGAGGCCTGG TCGCCGGGGC CGGGCCGGCA 
     1561 GCCATGGGCG TAGGAGCCCA GGTGGTGGCT CTGAGGCCAA CGGGCTGGCC CTGGTGTCCG 
     1621 GCTTTAAGCG GCTGCCACGG CAGGACGTGC AGATGAAGCC TGTGAAGTCA GTGCTGGTGA 
     1681 AGAGGAGAGA CAGCGAAGAG TTTGGCGTCA AGCTGGGCAG TCAGATCTTC ATCAAGCACA 
     1741 TTACAGATTC GGGCCTGGCT GCCCGGCACC GTGGGCTGCA GGAAGGAGAT CTCATTCTAC 
     1801 AGATCAACGG GGTGTCTAGC CAGAACCTGT CACTGAACGA CACCCGGCGA CTGATTGAGA 
     1861 AGTCAGAAGG GAAGCTAAGC CTGCTGGTGC TGAGAGATCG TGGGCAGTTC CTGGTGAACA 
     1921 TTCCGCCTGC TGTCAGTGAC AGCGACAGCT CGCCATTGGA GGAAGGCGTG ACCATGGCTG 
     1981 ATGAGATGTC CTCTCCCCCT GCAGACATCT CGGACCTCGC CTCGGAGCTA TCGCAGGCAC 
     2041 CACCATCCCA CATCCCACCA CCACCCCGGC ATGCTCAGCG GAGCCCCGAG GCCAGCCAGA 
     2101 CCGACTCTCC CGTGGAGAGT CCCCGGCTTC GGCGGGAAAG TTCAGTAGAT TCCAGAACCA 
     2161 TCTCGGAACC AGATGAGCAA CGGTCAGAGT TGCCCAGGGA AAGCAGCTAT GACATCTACA 
     2221 GAGTGCCCAG CAGTCAGAGC ATGGAGGATC GTGGGTACAG CCCCGACACG CGTGTGGTCC 
     2281 GCTTCCTCAA GGGCAAGAGC ATCGGGCTGC GGCTGGCAGG GGGCAATGAC GTGGGCATCT 
     2341 TCGTGTCCGG GGTGCAGGCG GGCAGCCCGG CCGACGGGCA GGGCATCCAG GAGGGAGATC 
     2401 AGATTCTGCA GGTGAATGAC GTGCCATTCC AGAACCTGAC ACGGGAGGAG GCAGTGCAGT 
     2461 TCCTGCTGGG GCTGCCACCA GGCGAGGAGA TGGAGCTGGT GACGCAGAGG AAGCAGGACA 
     2521 TTTTCTGGAA AATGGTGCAG TCCCGCGTGG GTGACTCCTT CTACATCCGC ACTCACTTTG 
     2581 AGCTGGAGCC CAGTCCACCG TCTGGCCTGG GCTTCACCCG TGGCGACGTC TTCCACGTGC 
     2641 TGGACACGCT GCACCCCGGC CCCGGGCAGA GCCACGCACG AGGAGGCCAC TGGCTGGCGG 
     2701 TGCGCATGGG TCGTGACCTG CGGGAGCAAG AGCGGGGCAT CATTCCCAAC CAGAGCAGGG 
     2761 CGGAGCAGCT GGCCAGCCTG GAAGCTGCCC AGAGGGCCGT GGGAGTCGGG CCCGGCTCCT 
     2821 CCGCGGGCTC CAATGCTCGG GCCGAGTTCT GGCGGCTGCG GGGTCTTCGT CGAGGAGCCA 
     2881 AGAAGACCAC TCAGCGGAGC CGTGAGGACC TCTCAGCTCT GACCCGACAG GGCCGCTACC 
     2941 CGCCCTACGA ACGAGTGGTG TTGCGAGAAG CCAGTTTCAA GCGCCCGGTA GTGATCCTGG 
     3001 GACCCGTGGC CGACATTGCT ATGCAGAAGT TGACTGCTGA GATGCCTGAC CAGTTTGAAA 
     3061 TCGCAGAGAC TGTGTCCAGG ACCGACAGCC CCTCCAAGAT CATCAAACTA GACACCGTGC 
     3121 GGGTGATTGC AGAAAAAGAC AAGCATGCGC TCCTGGATGT GACCCCCTCC GCCATCGAGC 
     3181 GCCTCAACTA TGTGCAGTAC TACCCCATTG TGGTCTTCTT CATCCCCGAG AGCCGGCCGG 
     3241 CCCTCAAGGC ACTGCGCCAG TGGCTGGCGC CTGCCTCCCG CCGCAGCACC CGTCGCCTCT 
     3301 ACGCACAAGC CCAGAAGCTG CGAAAACACA GCAGCCACCT CTTCACAGCC ACCATCCCTC 
     3361 TGAATGGCAC GAGTGACACC TGGTACCAGG AGCTCAAGGC CATCATTCGA GAGCAGCAGA 
     3421 CGCGGCCCAT CTGGACGGCG GAAGATCAGC TGGATGGCTC CTTGGAGGAC AACCTAGACC 
     3481 TCCCTCACCA CGGCCTGGCC GACAGCTCCG CTGACCTCAG CTGCGACAGC CGCGTTAACA 
     3541 GCGACTACGA GACGGACGGC GAGGGCGGCG CGTACACGGA TGGCGAGGGC TACACAGACG 
     3601 GCGAGGGGGG GCCCTACACG GATGTGGATG ATGAGCCCCC GGCTCCAGCC CTGGCCCGGT 
     3661 CCTCGGAGCC CGTGCAGGCA GATGAGTCCC AGAGCCCGAG GGATCGTGGG AGAATCTCGG 
     3721 CTCATCAGGG GGCCCAGGTG GACAGCCGCC ACCCCCAGGG ACAGTGGCGA CAGGACAGCA 
     3781 TGCGAACCTA TGAACGGGAA GCCCTGAAGA AAAAGTTTAT GCGAGTACAT GATGCGGAGT 
     3841 CCTCCGATGA AGACGGCTAT GACTGGGGTC CGGCCACTGA CCTGAGCGGA CCGACGCGTA 
     3901 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3961 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     4021 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     4081 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     4141 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4201 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4261 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4321 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4381 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4441 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4501 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4561 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5341 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5401 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5461 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5521 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5581 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5641 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5701 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5761 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5821 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5881 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5941 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     6001 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     6061 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     6121 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6181 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6241 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6301 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6361 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6421 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6481 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6541 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6601 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6661 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6721 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6781 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6961 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     7021 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     7081 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7141 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7201 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7261 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7321 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7381 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7441 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7501 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7561 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7621 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7681 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7741 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7801 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7861 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7921 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7981 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     8041 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     8101 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     8161 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8221 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8281 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8341 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8401 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8461 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8521 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8581 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8641 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8701 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8761 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8821 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8881 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8941 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     9001 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     9061 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     9121 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9181 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9241 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9301 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9361 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9421 ATTTT
//