LOCUS       RG213081                9362 bp    DNA     circular  SYN 
DEFINITION  OSBPL6 (tGFP-tagged) - Human oxysterol binding protein-like 6 (OSBPL6), transcript variant 1
ACCESSION   NM_032523
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3843..4547 
                     /label="GFP"  
     misc_feature    4594..5179 
                     /label="PolyA signal"  
     misc_feature    7271..7916 
                     /label="ColE1"  
     misc_feature    5633..6427 
                     /label="Neo"  
     misc_feature    5264..5566 
                     /label="SV40 ori"  
     misc_feature    7931..8791 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3822..3828 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3822 
                     /label="OSBPL6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCAGAT GAGAAGGGCA TTTCCCCTGC TCATAAAACA TCCACTCCAA 
     1081 CCCATAGAAG TGCCTCCTCT TCAACATCCT CCCAAAGGGA CAGTAGGCAG AGTATTCACA 
     1141 TACTGGAGAG GACTGCTTCC TCTAGCACCG AGCCCTCTGT AAGTCGGCAA TTGCTAGAAC 
     1201 CGGAGCCAGT CCCCCTCTCC AAGGAAGCTG ACAGCTGGGA AATTATAGAA GGGCTGAAAA 
     1261 TAGGCCAAAC CAATGTCCAG AAACCAGACA AACATGAGGG CTTTATGCTG AAGAAAAGAA 
     1321 AATGGCCTTT AAAAGGCTGG CACAAGCGTT TTTTTGTCCT GGATAATGGA ATGTTAAAGT 
     1381 ATTCAAAGGC ACCACTCGAT ATTCAGAAAG GAAAGGTCCA TGGGAGCATA GATGTGGGAC 
     1441 TCTCAGTCAT GTCAATTAAA AAGAAAGCTC GAAGAATAGA CCTTGACACC GAAGAGCACA 
     1501 TCTATCATTT GAAGGTGAAA TCCCAGGACT GGTTTGATGC ATGGGTCTCC AAACTGCGAC 
     1561 ATCATCGGTT GTATCGTCAG AATGAAATTG TGAGATCACC AAGAGATGCT AGTTTTCACA 
     1621 TATTTCCTTC AACGTCCACA GCTGAATCCT CACCAGCTGC TAATGTTTCT GTAATGGATG 
     1681 GAAAGATGCA ACCAAACAGC TTTCCGTGGC AGTCCCCTTT ACCATGCAGC AATAGCCTCC 
     1741 CTGCAACGTG CACAACTGGC CAGAGTAAAG TGGCAGCCTG GTTACAGGAC TCGGAAGAGA 
     1801 TGGACAGGTG TGCAGAAGAT CTTGCACATT GCCAGTCAAA CCTTGTGGAA CTTAGCAAAC 
     1861 TCCTGCAAAA TTTGGAAATA CTTCAGAGAA CTCAGTCAGC ACCTAACTTT ACTGACATGC 
     1921 AGGCTAACTG TGTAGATATT TCAAAGAAAG ACAAGCGGGT CACAAGACGA TGGAGAACAA 
     1981 AAAGTGTCAG CAAAGATACA AAAATACAAC TGCAGGTTCC TTTCAGTGCT ACCATGTCAC 
     2041 CAGTTCGCTT GCATTCCTCC AACCCCAACC TTTGTGCAGA TATTGAATTT CAGACTCCCC 
     2101 CTAGCCACCT CACTGACCCT CTGGAAAGTT CAACAGATTA TACAAAGCTG CAAGAAGAAT 
     2161 TTTGTCTAAT CGCACAGAAA GTGCATTCTC TTTTGAAGTC TGCATTTAAT AGCATAGCTA 
     2221 TAGAGAAGGA GAAGCTGAAG CAGATGGTTT CCGAGCAGGA TCACAGTAAA GGCCACAGCA 
     2281 CGCAGATGGC ACGGCTCCGA CAGTCACTGT CTCAGGCACT CAACCAGAAT GCTGAACTAA 
     2341 GGAGTCGGTT GAACAGAATA CATTCAGAGT CTATTATTTG TGATCAGGTT GTCAGTGTAA 
     2401 ATATTATTCC TAGCCCTGAT GAGGCTGGTG AGCAAATCCA TGTCAGTCTC CCCTTATCAC 
     2461 AGCAAGTAGC CAATGAGAGC CGCCTCTCCA TGTCAGAGTC TGTTTCTGAG TTCTTTGATG 
     2521 CCCAAGAGGT GCTCCTCTCT GCAAGTTCGT CAGAGAATGA GGCTTCAGAT GATGAGTCTT 
     2581 ACATCAGTGA TGTGAGTGAT AATATATCTG AAGACAACAC CAGTGTTGCA GACAATATTT 
     2641 CTCGGCAAAT CCTGAATGGG GAGCTTACAG GAGGGGCCTT CCGAAATGGG CGTCGAGCAT 
     2701 GCCTGCCAGC TCCTTGTCCT GACACCAGTA ACATTAACCT GTGGAATATC TTGAGGAACA 
     2761 ACATTGGTAA AGACCTGTCT AAAGTCTCTA TGCCTGTGGA GCTAAACGAG CCGCTCAACA 
     2821 CCCTGCAGCA CCTCTGTGAG GAAATGGAAT ACAGCGAGCT CCTGGACAAG GCTTCGGAAA 
     2881 CTGATGATCC ATATGAGCGC ATGGTTCTCG TTGCCGCATT TGCAGTTTCA GGATACTGCT 
     2941 CCACCTATTT CAGAGCAGGA AGTAAGCCAT TCAACCCAGT CCTTGGGGAG ACTTATGAAT 
     3001 GCATTAGAGA AGACAAGGGA TTCCGCTTTT TCTCAGAACA GGTTAGCCAT CATCCACCCA 
     3061 TTTCTGCCTG TCACTGTGAA TCAAAGAATT TTGTGTTTTG GCAAGATATC AGATGGAAAA 
     3121 ACAAGTTCTG GGGGAAGTCG ATGGAAATCC TGCCTGTTGG AACACTGAAT GTCATGCTTC 
     3181 CAAAGTATGG AGATTACTAT GTGTGGAATA AAGTCACCAC TTGCATACAC AACATCCTCA 
     3241 GTGGGAGAAG ATGGATAGAA CATTATGGAG AAGTAACCAT CAGAAATACC AAAAGCAGTG 
     3301 TTTGCATTTG CAAACTCACA TTTGTCAAGG TGAATTATTG GAATTCTAAC ATGAATGAAG 
     3361 TCCAGGGGGT GGTGATAGAT CAGGAGGGGA AGGCGGTGTA CCGGCTGTTT GGAAAGTGGC 
     3421 ATGAAGGACT CTACTGTGGT GTGGCCCCCT CTGCAAAGTG CATTTGGAGA CCAGGTTCCA 
     3481 TGCCAACAAA CTATGAGCTG TACTATGGCT TCACAAGGTT TGCTATTGAG CTCAATGAGT 
     3541 TAGATCCAGT ACTAAAAGAT CTCCTTCCAC CAACAGACGC CCGGTTCCGG CCAGATCAAA 
     3601 GATTTTTGGA AGAAGGAAAT TTAGAAGCTG CAGCATCAGA GAAGCAAAGA GTAGAGGAAC 
     3661 TCCAGAGATC TCGGAGACGA TATATGGAAG AAAACAATCT TGAACATATA CCAAAATTTT 
     3721 TTAAAAAAGT TATTGATGCC AATCAAAGAG AAGCCTGGGT TTCTAACGAC ACCTACTGGG 
     3781 AGCTTCGAAA GGACCCTGGG TTTAGCAAAG TAGACAGCCC TGTTCTTTGG ACGCGTACGC 
     3841 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3901 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3961 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     4021 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4081 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4141 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4201 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4261 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4321 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4381 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4441 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4501 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5281 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5341 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5401 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5461 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5521 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5581 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5641 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5701 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5761 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5821 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5881 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5941 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     6001 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6061 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6121 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6181 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6241 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6301 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6361 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6421 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6481 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6541 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6601 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6661 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6721 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6781 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6841 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6901 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6961 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     7021 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7081 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7141 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7201 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7261 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7321 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7381 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7441 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7501 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7561 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7621 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7681 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7741 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7801 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7861 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7921 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7981 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     8041 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8101 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8161 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8221 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8281 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8341 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8401 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8461 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8521 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8581 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8641 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8701 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8761 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8821 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8881 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8941 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     9001 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9061 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9121 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9181 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9241 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9301 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9361 TT
//