LOCUS       RG212488                8914 bp    DNA     circular  SYN 
DEFINITION  ZFYVE20 (tGFP-tagged) - Human zinc finger, FYVE domain containing 20 (ZFYVE20)
ACCESSION   NM_022340
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3395..4099 
                     /label="GFP"  
     misc_feature    4146..4731 
                     /label="PolyA signal"  
     misc_feature    6823..7468 
                     /label="ColE1"  
     misc_feature    5185..5979 
                     /label="Neo"  
     misc_feature    4816..5118 
                     /label="SV40 ori"  
     misc_feature    7483..8343 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3374..3380 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3374 
                     /label="RBSN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCTCTG GACGACCCAG GGGAAGTGAG GGAGGGCTTC CTCTGCCCTC 
     1081 TGTGCCTGAA GGATCTGCAG TCTTTCTATC AGCTTCACTC ACATTACGAG GAAGAACACT 
     1141 CAGGGGAAGA CCGTGATGTC AAAGGGCAAA TTAAAAGTCT TGTCCAGAAG GCTAAAAAAG 
     1201 CAAAGGACAG GTTGTTGAAA CGAGAAGGGG ATGATCGAGC AGAGTCAGGG ACCCAAGGAT 
     1261 ATGAGTCTTT CAGCTATGGA GGGGTTGATC CTTACATGTG GGAACCCCAG GAGCTTGGTG 
     1321 CTGTGAGAAG CCATCTTTCC GACTTCAAAA AACACCGAGC TGCTAGAATT GACCACTATG 
     1381 TTGTGGAAGT CAATAAACTA ATAATCAGGT TAGAGAAGCT CACTGCATTT GACAGAACAA 
     1441 ATACTGAGTC TGCAAAGATT CGAGCAATAG AAAAGTCTGT GGTGCCTTGG GTCAACGACC 
     1501 AGGATGTCCC TTTCTGTCCA GACTGTGGGA ATAAGTTCAG CATCCGGAAC CGCCGCCACC 
     1561 ACTGCCGCCT CTGCGGGTCT ATTATGTGCA AGAAGTGTAT GGAGCTCATC AGCCTTCCCT 
     1621 TGGCAAACAA GCTCACCAGT GCCAGCAAGG AGTCCCTGAG CACCCACACC AGCCCCAGCC 
     1681 AGTCACCCAA CAGTGTCCAT GGCTCCCGCC GAGGCAGCAT CAGCAGCATG AGCAGTGTCA 
     1741 GCTCGGTCCT GGATGAGAAG GACGATGACC GGATCCGCTG CTGTACACAC TGCAAGGACA 
     1801 CGCTGCTCAA GAGAGAGCAG CAGATTGATG AGAAGGAGCA CACACCTGAC ATCGTGAAGC 
     1861 TCTACGAGAA ATTACGACTT TGCATGGAGA AAGTTGACCA GAAAGCTCCA GAATACATCA 
     1921 GGATGGCAGC ATCATTAAAT GCTGGGGAGA CAACCTACAG TCTGGAACAT GCCAGTGACC 
     1981 TTCGAGTGGA AGTGCAGAAA GTGTATGAGC TGATAGACGC TTTAAGTAAG AAGATCTTAA 
     2041 CCTTGGGCTT GAACCAGGAC CCTCCACCAC ATCCAAGCAA TTTGCGGCTG CAGAGAATGA 
     2101 TCAGATACTC AGCTACACTT TTTGTGCAGG AAAAGTTGCT TGGTTTGATG TCACTGCCAA 
     2161 CCAAAGAACA GTTTGAGGAA CTGAAAAAGA AAAGGAAGGA GGAAATGGAG AGGAAGAGGG 
     2221 CCGTGGAGAG ACAAGCTGCC CTGGAGTCCC AGCGAAGGCT TGAGGAAAGG CAGAGTGGCC 
     2281 TGGCTTCTCG AGCGGCCAAC GGGGAGGTGG CATCTCTCCG CAGGGGCCCT GCCCCCTTGA 
     2341 GAAAGGCTGA GGGCTGGCTC CCACTGTCAG GAGGTCAGGG GCAGAGTGAG GACTCAGACC 
     2401 CGCTCCTCCA GCAGATCCAC AACATCACAT CATTCATCAG GCAGGCCAAG GCCGCGGGCC 
     2461 GCATGGATGA AGTGCGCACT CTGCAGGAGA ACCTGCGGCA GCTGCAGGAC GAGTATGACC 
     2521 AGCAGCAGAC AGAGAAGGCC ATCGAGCTGT CCCGGAGGCA GGCTGAGGAG GAGGACCTGC 
     2581 AGCGGGAACA GCTGCAGATG TTGCGTGAAC GGGAGTTGGA ACGAGAAAGG GAGCAGTTTC 
     2641 GGGTGGCATC CCTGCACACA CGGACTCGGT CCCTGGACTT CAGAGAAATC GGCCCTTTTC 
     2701 AGCTGGAGCC CAGCAGAGAG CCTCGCACCC ACCTTGCTTA TGCTTTGGAT CTAGGCTCTT 
     2761 CCCCAGTTCC AAGCAGCACA GCTCCCAAGA CCCCTTCACT TAGCTCAACT CAACCCACCA 
     2821 GAGTGTGGTC TGGGCCCCCA GCCGTTGGCC AGGAGCGCTT ACCCCAGAGC AGCATGCCAC 
     2881 AGCAACATGA GGGGCCCTCC TTAAACCCCT TTGATGAGGA AGACCTCTCC AGCCCCATGG 
     2941 AAGAGGCCAC TACTGGTCCT CCTGCTGCAG GGGTTTCCTT AGACCCTTCA GCCCGCATCC 
     3001 TGAAAGAGTA CAATCCTTTC GAGGAAGAGG ACGAGGAGGA GGAAGCAGTG GCAGGGAATC 
     3061 CATTCATTCA GCCAGACAGC CCAGCTCCTA ACCCCTTCAG TGAGGAAGAC GAACATCCCC 
     3121 AGCAGAGGCT CTCAAGCCCT CTGGTTCCTG GTAACCCCTT TGAGGAACCC ACCTGTATCA 
     3181 ACCCCTTTGA GATGGACAGT GACAGTGGGC CAGAGGCTGA GGAGCCCATA GAGGAAGAGC 
     3241 TCCTCCTGCA GCAGATCGAT AACATCAAGG CATACATCTT TGATGCCAAG CAGTGCGGCC 
     3301 GCCTGGATGA GGTAGAGGTG CTGACAGAGA ATCTGCGGGA GCTGAAGCAC ACCCTGGCCA 
     3361 AGCAGAAGGG GGGCACTGAC TGACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG 
     3421 CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT 
     3481 CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA 
     3541 GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG 
     3601 CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA 
     3661 CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT 
     3721 GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC 
     3781 CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT 
     3841 GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG 
     3901 CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC 
     3961 CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA 
     4021 GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA 
     4081 TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA 
     4141 CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG 
     4201 CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT 
     4261 AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG 
     4321 GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC 
     4381 AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC 
     4441 CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT 
     4501 AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT 
     4561 ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG 
     4621 TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC 
     4681 CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG 
     4741 TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG 
     4801 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 
     4861 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 
     4921 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 
     4981 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 
     5041 TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 
     5101 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 
     5161 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 
     5221 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 
     5281 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 
     5341 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 
     5401 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 
     5461 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 
     5521 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 
     5581 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 
     5641 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 
     5701 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 
     5761 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 
     5821 GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 
     5881 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 
     5941 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 
     6001 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 
     6061 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 
     6121 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 
     6181 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 
     6241 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 
     6301 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 
     6361 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 
     6421 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 
     6481 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 
     6541 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 
     6601 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6661 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6721 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6781 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6841 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6901 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6961 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     7021 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     7081 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     7141 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     7201 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     7261 CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7321 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7381 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7441 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 
     7501 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 
     7561 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 
     7621 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 
     7681 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 
     7741 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 
     7801 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 
     7861 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 
     7921 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 
     7981 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 
     8041 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 
     8101 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 
     8161 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 
     8221 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 
     8281 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 
     8341 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     8401 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     8461 ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 
     8521 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 
     8581 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 
     8641 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 
     8701 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 
     8761 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 
     8821 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 
     8881 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//