LOCUS       RG210931                9089 bp    DNA     circular  SYN 
DEFINITION  AXIN2 (tGFP-tagged) - Human axin 2 (AXIN2)
ACCESSION   NM_004655
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3570..4274 
                     /label="GFP"  
     misc_feature    4321..4906 
                     /label="PolyA signal"  
     misc_feature    6998..7643 
                     /label="ColE1"  
     misc_feature    5360..6154 
                     /label="Neo"  
     misc_feature    4991..5293 
                     /label="SV40 ori"  
     misc_feature    7658..8518 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3549..3555 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3549 
                     /label="AXIN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTAGCGCT ATGTTGGTGA CTTGCCTCCC GGACCCCAGC AGCAGCTTCC 
     1081 GTGAGGATGC CCCGCGGCCC CCAGTGCCAG GGGAAGAAGG GGAGACCCCA CCGTGTCAGC 
     1141 CAGGGGTGGG CAAGGGCCAG GTCACCAAAC CCATGTCTGT CTCTTCCAAC ACCAGGCGGA 
     1201 ACGAAGATGG GTTGGGGGAG CCGGAGGGGC GGGCATCTCC GGATTCCCCT CTGACCCGGT 
     1261 GGACCAAGTC CTTACACTCC TTATTGGGCG ATCAAGACGG TGCTTACCTG TTCCGAACTT 
     1321 TCCTGGAGAG GGAGAAATGC GTGGATACCT TAGACTTCTG GTTTGCCTGC AATGGATTCA 
     1381 GGCAGATGAA CCTGAAGGAT ACCAAAACTT TACGAGTAGC CAAAGCGATC TACAAAAGGT 
     1441 ACATTGAGAA CAACAGCATT GTCTCCAAGC AGCTGAAGCC TGCCACCAAG ACCTACATAA 
     1501 GAGATGGCAT CAAGAAGCAG CAGATTGATT CCATCATGTT TGACCAGGCG CAGACCGAGA 
     1561 TCCAGTCGGT GATGGAGGAA AATGCCTACC AGATGTTTTT GACTTCTGAT ATATACCTCG 
     1621 AATATGTGAG GAGTGGGGGA GAAAACACAG CTTACATGAG TAATGGGGGA CTCGGGAGCC 
     1681 TAAAGGTCGT GTGTGGCTAT CTCCCCACCT TGAATGAAGA AGAGGAGTGG ACTTGTGCCG 
     1741 ACTTCAAGTG CAAACTTTCG CCAACCGTGG TTGGCTTGTC CAGCAAAACT CTGAGGGCCA 
     1801 CGGCGAGTGT GAGGTCCACG GAAACTGTTG ACAGTGGATA CAGGTCCTTC AAGAGGAGCG 
     1861 ATCCTGTTAA TCCTTATCAC ATAGGTTCTG GCTATGTCTT TGCACCAGCC ACCAGCGCCA 
     1921 ACGACAGTGA GATATCCAGT GATGCGCTGA CGGATGATTC CATGTCCATG ACGGACAGCA 
     1981 GTGTAGATGG AATTCCTCCT TATCGTGTGG GCAGTAAGAA ACAGCTCCAG AGAGAAATGC 
     2041 ATCGCAGTGT GAAGGCCAAT GGCCAAGTGT CTCTACCTCA TTTCCCGAGA ACCCACCGCC 
     2101 TGCCCAAGGA GATGACCCCC GTGGAACCCG CCACCTTTGC AGCTGAGCTG ATCTCGAGGC 
     2161 TGGAAAAGCT GAAGCTGGAG TTGGAGAGCC GCCACAGCCT GGAGGAGCGC CTGCAGCAGA 
     2221 TCCGAGAGGA TGAAGAGAGA GAGGGCTCCG AGCTCACACT CAATTCGCGG GAGGGGGCGC 
     2281 CCACGCAGCA CCCCCTCTCC CTACTGCCCT CCGGCAGCTA CGAGGAAGAC CCGCAGACGA 
     2341 TACTGGACGA TCACCTGTCC AGGGTCCTCA AGACCCCTGG CTGCCAGTCT CCGGGCGTAG 
     2401 GCCGCTATAG CCCTCGCTCC CGCTCCCCGG ACCACCACCA CCACCACCAT TCGCAGTACC 
     2461 ACTCCCTGCT CCCGCCCGGT GGCAAGCTGC CTCCCGCGGC CGCCTCGCCG GGCGCCTGCC 
     2521 CCCTCCTCGG GGGCAAAGGC TTTGTGACCA AGCAGACGAC GAAGCATGTC CACCACCACT 
     2581 ACATCCACCA CCATGCCGTC CCCAAGACCA AGGAGGAGAT CGAGGCGGAG GCCACGCAGC 
     2641 GGGTGCACTG CTTCTGCCCT GGGGGCAGCG AGTATTACTG CTACTCGAAA TGCAAAAGCC 
     2701 ACTCCAAGGC TCCGGAAACC ATGCCCAGCG AGCAGTTTGG CGGCAGCAGA GGCAGTACCT 
     2761 TGCCCAAACG CAATGGGAAA GGCACGGAGC CGGGCCTGGC CCTGCCCGCC AGGGAAGGAG 
     2821 GGGCCCCCGG CGGAGCTGGG GCCCTGCAGC TTCCCCGGGA GGAAGGAGAC AGGTCGCAGG 
     2881 ATGTCTGGCA GTGGATGCTG GAGAGTGAGC GGCAGAGCAA GCCCAAGCCC CATAGTGCCC 
     2941 AAAGCACAAA AAAGGCCTAC CCCTTGGAGT CTGCCCGCTC GTCTCCAGGC GAACGAGCCA 
     3001 GCCGGCACCA TCTGTGGGGG GGCAACAGCG GGCACCCCCG CACCACCCCC CGTGCCCACC 
     3061 TGTTCACCCA GGACCCTGCG ATGCCTCCCC TGACCCCACC CAACACGCTG GCTCAGCTGG 
     3121 AGGAGGCCTG TCGCAGGCTA GCTGAGGTGT CGAAGCCCCC AAAGCAGCGG TGCTGTGTGG 
     3181 CCAGTCAGCA GAGGGACAGG AATCATTCGG CCACTGTTCA GACGGGAGCC ACACCCTTCT 
     3241 CCAATCCAAG CCTGGCTCCA GAAGATCACA AAGAGCCAAA GAAACTGGCA GGTGTCCACG 
     3301 CGCTCCAGGC CAGTGAGTTG GTTGTCACTT ACTTTTTCTG TGGGGAAGAA ATTCCATACC 
     3361 GGAGGATGCT GAAGGCTCAG AGCTTGACCC TGGGCCACTT TAAAGAGCAG CTCAGCAAAA 
     3421 AGGGAAATTA TAGGTATTAC TTCAAAAAAG CAAGCGATGA GTTTGCCTGT GGAGCGGTGT 
     3481 TTGAGGAGAT CTGGGAGGAT GAGACGGTGC TCCCGATGTA TGAAGGCCGG ATTCTGGGCA 
     3541 AAGTGGAGCG GATCGATACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3601 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3661 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3721 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3781 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3841 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     3901 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     3961 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4021 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4081 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4141 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4201 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4261 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4321 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4381 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4441 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4501 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4561 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4621 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4681 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4741 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4801 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4861 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4921 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     4981 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5041 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5101 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5161 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5221 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5281 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5341 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5401 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5461 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5521 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5581 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5641 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5701 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5761 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5821 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     5881 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     5941 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6001 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6061 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6121 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6181 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6241 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6301 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6361 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6421 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6481 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6541 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6601 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6661 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6721 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6781 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6841 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6901 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     6961 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7021 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7081 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7141 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7201 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7261 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7321 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7381 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7441 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7501 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7561 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7621 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7681 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7741 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7801 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7861 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     7921 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     7981 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8041 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8101 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8161 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8221 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8281 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8341 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8401 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8461 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8521 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8581 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8641 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8701 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8761 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8821 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     8881 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     8941 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9001 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9061 TGATTTAACA AAAATTTAAC GCGAATTTT
//