LOCUS       RG210863                9029 bp    DNA     circular  SYN 
DEFINITION  PCDHGA2 (tGFP-tagged) - Human protocadherin gamma subfamily A, 2 (PCDHGA2), transcript variant 2
ACCESSION   NM_032009
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3510..4214 
                     /label="GFP"  
     misc_feature    4261..4846 
                     /label="PolyA signal"  
     misc_feature    6938..7583 
                     /label="ColE1"  
     misc_feature    5300..6094 
                     /label="Neo"  
     misc_feature    4931..5233 
                     /label="SV40 ori"  
     misc_feature    7598..8458 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3489..3495 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3489 
                     /label="PCDHGA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCTCTG CAAAAGTTGC CACACTGCAG AAAGCTGGTC CTGCTGTGCT 
     1081 TCCTTTTGGC GACCCTGTGG GAGGCCAGGG CCGGGCAGAT TCGCTATTCT GTGCGGGAAG 
     1141 AGATCGACAG AGGCTCCTTC GTAGGCAACA TCGCCAAGGA CTTGGGTTTG GAGCCCCTGG 
     1201 CACTGGCAGA GCAGGGAGTC CGCATCGTCT CCAGAGGTAG GTCCCAGCTC TTTGCTCTGA 
     1261 ACCCGCGAAG CGGCAGCTTG GTCACTGCGA ACAGGATAGA CCGGGAGGAG CTCTGCGCTC 
     1321 AGAGCGCACC CTGTCTGTTG AATTTTAACA TTCTGCTGGA GGATAAATTG ACTATTTATT 
     1381 CAGTAGAGGT GGAAATAACA GATATTAACG ATAATGCCCC TCGCTTTGGA GTAGAGGAAC 
     1441 TGGAGCTAAA AATCAGTGAA ACCACTACGC CAGGATTCCG GATTCCTCTT AAGAATGCGC 
     1501 ATGATGCAGA CGTAGGTGAG AACGCCCTTC AGAAGTACGC ACTCAACCCA AATGACCACT 
     1561 TCTCCCTGGA CGTGCGAAGA GGAGCTGATG GGAACAAGTA CCCAGAACTG GTGCTGGAGC 
     1621 GCTCTCTGGA CCGCGAGGAA GAGGCTGTTC ACCACCTCGT TCTCGTGGCT TCTGATGGGG 
     1681 GTGACCCAGT GCTATCTGGC ACCTCCCGCA TCTGCGTGAA GGTCCTGGAT GCGAACGACA 
     1741 ATGCGCCTGT TTTTACACAG CCCGAGTACC GCATAAGCAT TCCGGAGAAT ACGCTCGTGG 
     1801 GCACCCGGAT ACTCACGGTG ACCGCCACTG ACGCAGATGA GGGCTACTAC GCTCAAGTGG 
     1861 TATATTTTCT AGAGAAAAGC CCTGGAGAAA CCTCAGAGGT ATTTGAGCTT AAGTCAACAT 
     1921 CTGGAGAACT GACAATCATA AAAGATCTAG ATTATGAGGA TGCTACATTC CATGAAATTG 
     1981 ATATTGAAGC TCAGGATGGT CCGGGCCTTC TAACCAGAGC GAAGGTTATC GTCACGGTTC 
     2041 TGGATGTGAA TGACAATGCC CCAGAATTTT ACATGACATC TGCTACTAGC TCAGTTTCTG 
     2101 AAGACTCTCT TCCAGGAACC ATAATTGGGC TTTTTAATGT ACATGATAGA GACTCTGGGC 
     2161 AGAACGCATT CACCACCTGT TCACTCCCCG AGGATCTTCC TTTTAAGTTA GAAAAGTCAG 
     2221 TAGACAATTA CTACCGACTG GTTACAACCA GAGCCCTTGA CAGGGAACAG TTTTCCTTTT 
     2281 ACAACATCAC TCTAACCGCT AAAGATGGAG GGAACCCCTC CCTGTCCACG GATGCTCACA 
     2341 TTTTGCTCCA GGTGGCAGAC ATCAACGACA ACGCACCCGC CTTCTCCCGC ACATCCTACT 
     2401 CCACCTACAT TCCCGAAAAC AACCCCAGAG GAGCCTCTGT CTTCTCAGTG ACGGCCCATG 
     2461 ACCCCGACAG CAACGACAAT GCTCATGTAA CTTACTCTTT CGCGGAGGAC ACTGTTCAGG 
     2521 GGGCACCCTT ATCCTCTTAC ATCTCTATCA ACTCCGACAC TGGAGTACTC TATGCACTGC 
     2581 GCTCCTTTGA TTATGAGCAG TTGCGAGACT TGCAAGTGTG GGTGATAGCG CGGGACAGCG 
     2641 GGAACCCTCC ACTCAGTAGC AATGTATCAT TAAGCCTGTT CGTGCTGGAC CAGAACGACA 
     2701 ACGCGCCCGA GATCCTGTAC CCTGCCTTCC CCACAGACGG TTCCACTGGC GTGGAGCTGG 
     2761 CGCCCCGCTC CGCAGAGCCC GGCTACCTGG TGACCAAGGT GGTGGCGGTG GACAGAGACT 
     2821 CGGGCCAGAA CGCCTGGCTG TCTTACCACC TGCTCAAGGC CAGCGAGCCG GGACTCTTCT 
     2881 CGGTGGGTCT GCACACGGGC GAGGTGCGCA CGGCGCGAGC CCTGCTGGAC AGAGACGCGC 
     2941 TCAAGCAGAG CCTCGTGGTG GCCATCCAGG ACCACGGCCA GCCCCCTCTC TCCGCCACTG 
     3001 TCACGCTCAC CGTGGCCGTG GCCGACAGGA TCCCCGACAT CCTGGCCGAC CTGGGCAGCC 
     3061 TCGAGCCCTC CGCCATACCC AACGATTCGG ACCTCACTCT GTACCTGGTG GTGGCGGTGG 
     3121 CCGCGGTCTC CTGCGTCTTC CTGGCCTTCG TCATCGTGTT GCTGGCGCAC AGGCTGCGGC 
     3181 GCTGGCACAA GTCACGCCTG CTGCAGGCTT CAGGAGGCAG CTTGACAGGC ATGCAGAGCT 
     3241 CGCACTTTGT GGGCGTGGAC GGGGTTCGGG CTTTCCTGCA GACCTATTCC CACGAGGTCT 
     3301 CCCTCACTGC GGACTCGCGG AAGAGCCACC TGATTTTCCC CCAGCCCAAC TATGCGGACA 
     3361 CGCTCATCAG CCAGGAGAGC TGTGAGAAAA AGGATTTTTT ATCAGCGCCT CAATCTCTAC 
     3421 TCGAAGAAGA AAGAGAAGAA ACGTTTTCTC AGGTAATCTA TCTTTTCACA ACATACGTAC 
     3481 TAGCTAGTTT GCTGAAGACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3541 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3601 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3661 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3721 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3781 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     3841 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     3901 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     3961 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4021 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4081 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4141 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4201 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4261 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4321 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4381 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4441 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4501 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4561 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4621 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4681 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4741 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4801 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4861 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     4921 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4981 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5041 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5101 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5161 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5221 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5281 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5341 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5401 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5461 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5521 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5581 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5641 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5701 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5761 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     5821 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     5881 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     5941 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6001 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6061 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6121 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6181 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6241 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6301 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6361 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6421 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6481 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6541 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6601 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6661 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6721 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6781 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6841 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     6901 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     6961 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7021 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7081 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7141 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7201 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7261 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7321 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7381 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7441 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7501 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7561 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7621 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7681 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7741 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7801 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     7861 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     7921 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     7981 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8041 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8101 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8161 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8221 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8281 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8341 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8401 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8461 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8521 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8581 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8641 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8701 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8761 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     8821 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     8881 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     8941 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9001 TGATTTAACA AAAATTTAAC GCGAATTTT
//