LOCUS       RG210521                9431 bp    DNA     circular  SYN 
DEFINITION  ENPEP (tGFP-tagged) - Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP)
ACCESSION   NM_001977
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3912..4616 
                     /label="GFP"  
     misc_feature    4663..5248 
                     /label="PolyA signal"  
     misc_feature    7340..7985 
                     /label="ColE1"  
     misc_feature    5702..6496 
                     /label="Neo"  
     misc_feature    5333..5635 
                     /label="SV40 ori"  
     misc_feature    8000..8860 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3891..3897 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3891 
                     /label="ENPEP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTTGCG GAGAGAGAGG GCTCTAAGAG ATACTGCATT CAAACGAAAC 
     1081 ATGTGGCCAT TCTCTGTGCG GTGGTGGTGG GTGTAGGATT AATAGTGGGA CTTGCCGTGG 
     1141 GCTTGACCAG ATCGTGTGAC TCCAGCGGGG ACGGCGGGCC GGGCACTGCG CCAGCTCCTT 
     1201 CCCACCTGCC TTCTTCCACG GCCAGCCCCT CAGGTCCTCC TGCCCAGGAC CAGGACATCT 
     1261 GCCCGGCCAG TGAGGATGAG AGCGGACAGT GGAAAAACTT TCGACTGCCG GACTTCGTCA 
     1321 ACCCAGTCCA CTACGACCTG CACGTGAAGC CCCTGTTGGA GGAGGACACC TACACGGGCA 
     1381 CCGTGAGCAT CTCCATCAAC CTGAGCGCTC CCACCCGGTA CCTGTGGCTG CACCTCCGGG 
     1441 AGACCAGGAT CACCCGGCTC CCGGAGCTGA AGAGGCCCTC TGGGGACCAG GTGCAAGTCC 
     1501 GGAGGTGTTT CGAGTACAAA AAGCAGGAGT ACGTGGTGGT CGAGGCGGAG GAAGAGCTTA 
     1561 CCCCCAGCAG TGGAGATGGC CTGTATCTCC TGACCATGGA GTTCGCCGGC TGGCTGAACG 
     1621 GCTCCCTCGT GGGATTTTAT AGAACCACCT ACACGGAGAA CGGACGAGTC AAGAGCATAG 
     1681 TGGCCACCGA TCATGAACCA ACAGATGCCA GGAAATCTTT TCCTTGTTTT GATGAGCCCA 
     1741 ACAAAAAGGC AACTTATACA ATATCTATCA CCCATCCCAA AGAATACGGA GCACTTTCAA 
     1801 ATATGCCAGT GGCGAAAGAA GAGTCAGTGG ATGATAAATG GACTCGAACA ACTTTTGAGA 
     1861 AGTCTGTCCC CATGAGCACG TACCTGGTGT GCTTTGCTGT ACATCAATTT GACTCTGTAA 
     1921 AGAGAATATC AAATAGTGGA AAACCTCTTA CAATTTATGT CCAGCCAGAG CAAAAGCACA 
     1981 CAGCCGAATA TGCTGCAAAC ATAACTAAAA GTGTGTTTGA TTATTTTGAA GAATACTTTG 
     2041 CTATGAATTA TTCTCTTCCT AAATTAGATA AAATCGCTAT TCCAGATTTT GGCACTGGTG 
     2101 CCATGGAGAA CTGGGGACTC ATCACGTACA GAGAAACGAA CCTGCTTTAT GACCCTAAGG 
     2161 AATCAGCCTC ATCAAACCAA CAGAGGGTGG CCACTGTGGT TGCCCATGAA CTTGTGCATC 
     2221 AGTGGTTTGG AAATATTGTG ACCATGGACT GGTGGGAAGA CTTGTGGCTA AATGAAGGAT 
     2281 TTGCTTCTTT CTTTGAGTTT CTGGGAGTAA ACCATGCAGA AACAGACTGG CAAATGCGTG 
     2341 ACCAAATGTT ACTTGAAGAT GTATTACCTG TTCAAGAGGA TGATTCTTTG ATGTCTTCGC 
     2401 ATCCAATTAT TGTGACTGTG ACAACCCCTG ATGAAATAAC ATCTGTTTTT GATGGAATAT 
     2461 CCTATAGCAA GGGATCTTCT ATTTTGAGAA TGCTTGAAGA CTGGATAAAA CCAGAGAATT 
     2521 TTCAAAAAGG ATGTCAGATG TACTTGGAAA AATACCAATT CAAGAATGCA AAAACTTCTG 
     2581 ATTTTTGGGC AGCACTGGAA GAGGCAAGTA GGCTACCAGT GAAAGAAGTA ATGGACACCT 
     2641 GGACCAGACA GATGGGTTAT CCTGTGCTTA ACGTGAACGG TGTCAAGAAC ATCACACAGA 
     2701 AACGCTTTTT GTTGGACCCA AGAGCTAACC CTTCTCAGCC CCCTTCAGAT CTTGGTTATA 
     2761 CATGGAATAT CCCAGTTAAA TGGACTGAAG ATAATATAAC AAGCAGTGTG TTATTTAATA 
     2821 GGTCAGAAAA AGAAGGAATC ACTTTGAACT CCTCTAATCC TAGTGGAAAT GCTTTTCTCA 
     2881 AAATAAACCC AGATCATATT GGGTTTTATC GTGTAAATTA TGAAGTAGCA ACTTGGGACT 
     2941 CGATAGCTAC AGCGCTCTCC TTGAACCACA AGACATTTTC TTCAGCAGAT CGTGCAAGTC 
     3001 TTATTGATGA TGCTTTTGCC TTGGCAAGAG CTCAACTTCT AGATTATAAG GTGGCTTTGA 
     3061 ACTTGACCAA GTATCTCAAA AGGGAAGAGA ATTTTTTACC ATGGCAGAGA GTAATTTCAG 
     3121 CTGTAACCTA CATCATTAGC ATGTTTGAAG ATGATAAAGA GCTATATCCT ATGATTGAGG 
     3181 AATACTTCCA AGGTCAAGTG AAGCCTATTG CAGATTCTCT GGGATGGAAT GATGCTGGAG 
     3241 ACCATGTCAC AAAGTTACTC CGTTCCTCCG TGTTAGGGTT TGCGTGCAAG ATGGGAGACA 
     3301 GAGAAGCCTT GAACAATGCT TCCTCGTTAT TTGAGCAGTG GCTAAATGGG ACTGTAAGCC 
     3361 TTCCCGTAAA TCTCAGGCTT CTGGTGTATC GGTATGGGAT GCAGAACTCT GGCAATGAGA 
     3421 TTTCATGGAA CTACACTCTT GAGCAATACC AGAAAACTTC ATTAGCTCAA GAAAAAGAAA 
     3481 AACTGCTGTA TGGATTAGCA TCAGTGAAGA ACGTTACTCT TTTGTCAAGG TATTTGGATT 
     3541 TGCTCAAGGA CACGAACCTT ATTAAAACTC AGGATGTGTT TACAGTCATT CGATATATCT 
     3601 CATATAACAG CTATGGGAAG AACATGGCCT GGAATTGGAT ACAACTCAAC TGGGACTATC 
     3661 TAGTCAACAG ATATACACTC AATAACAGAA ACCTTGGCCG AATTGTCACA ATAGCAGAGC 
     3721 CATTCAACAC TGAACTGCAA CTGTGGCAGA TGGAGAGCTT TTTTGCAAAA TATCCACAAG 
     3781 CTGGAGCAGG AGAAAAACCT AGGGAACAAG TGCTGGAAAC AGTGAAAAAC AATATAGAGT 
     3841 GGCTAAAACA ACATAGAAAC ACCATCAGAG AATGGTTTTT TAATTTACTT GAGAGTGGTA 
     3901 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     3961 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     4021 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     4081 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     4141 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     4201 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     4261 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     4321 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     4381 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     4441 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     4501 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4561 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     5341 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5401 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5461 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     5521 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5581 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5641 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5701 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5761 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5821 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5881 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     5941 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     6001 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     6061 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     6121 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     6181 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     6241 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     6301 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     6361 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     6421 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     6481 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6541 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     6961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     7021 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     7081 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     7141 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     7201 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     7261 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     7321 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     7381 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     7441 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     7501 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7561 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7621 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7681 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7741 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7801 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7861 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7921 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     7981 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     8041 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     8101 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     8161 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     8221 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     8281 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     8341 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     8401 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     8461 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     8521 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8581 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8641 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8701 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8761 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8821 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8881 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     8941 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     9001 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     9061 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     9121 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     9181 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     9241 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     9301 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     9361 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     9421 ACGCGAATTT T
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