LOCUS       RG210509                9233 bp    DNA     circular  SYN 
DEFINITION  PPP6R1 (tGFP-tagged) - Human protein phosphatase 6, regulatory subunit 1 (PPP6R1)
ACCESSION   NM_014931
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3714..4418 
                     /label="GFP"  
     misc_feature    4465..5050 
                     /label="PolyA signal"  
     misc_feature    7142..7787 
                     /label="ColE1"  
     misc_feature    5504..6298 
                     /label="Neo"  
     misc_feature    5135..5437 
                     /label="SV40 ori"  
     misc_feature    7802..8662 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3693..3699 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3693 
                     /label="PPP6R1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCGCCGC CCCCCCTCGC AGGGCGCCAT GTTTTGGAAG TTTGACCTGC 
     1081 ACACAAGCTC GCACCTGGAC ACGCTGCTGG AGCGGGAGGA CCTGAGCCTG CCCGAGCTGC 
     1141 TGGACGAGGA AGACGTGCTG CAGGAGTGCA AGGTCGTCAA CCGCAAGCTG CTGGACTTCC 
     1201 TGCTGCAGCC ACCCCACCTG CAAGCAATGG TGGCCTGGGT CACCCAGGAG CCGCCAGATA 
     1261 GCGGTGAGGA GCGGCTGCGC TACAAGTACC CCAGTGTGGC CTGCGAGATT CTGACCTCAG 
     1321 ATGTGCCCCA GATCAATGAT GCCCTGGGTG CCGATGAGTC CCTTCTGAAC CGGCTCTACG 
     1381 GCTTCCTGCA GAGCACCGGC AGCCTCAACC CACTGCTGGC CAGCTTCTTC AGCAAGGTCA 
     1441 TGGGCATCCT CATCAACCGC AAGACAGACC AGCTCGTGTC CTTTCTTCGG AAGAAGGATG 
     1501 ACTTCGTGGA CCTGCTGCTG CAGCACATTG GCACCTCGGC CATCATGGAC CTCCTGCTGC 
     1561 GCCTGCTCAC CTGTGTGGAG CGGCCTCAGC TGAGGCAGGA TGTTGTCAAT TGGCTCAACG 
     1621 AGGAGAAGAT CGTCCAGCGG CTGATTGAGC AGATCCACCC GTCGAAGGAT GAGAATCAAC 
     1681 ATTCCAACGC ATCCCAGTCC CTGTGTGACA TCATCCGCCT GAGCCGGGAG CAGATGATCC 
     1741 AAGTCCAGGA CAGCCCAGAG CCTGACCAAC TGCTGGCCAC CCTGGAGAAG CAGGAGACGA 
     1801 TTGAGCAGCT CTTAAGCAAC ATGTTCGAGG GGGAGCAGAG CCAGTCTGTC ATCGTCAGTG 
     1861 GGATCCAGGT GCTGCTGACC CTGCTGGAGC CCAGGAGGCC GAGGTCCGAG TCCGTGACCG 
     1921 TGAACAGCTT CTTCAGCAGT GTGGATGGGC AGCTGGAGCT CCTGGCCCAG GGGGCCCTGG 
     1981 AAAGCACTGT GTCCAGTGTG GGCGCCTTGC ACGCCCTACG CCCGCGGCTC AGCTGCTTCC 
     2041 ACCAGCTCCT GCTGGAGCCT CCCAAGCTGG AGCCGCTACA GATGACATGG GGCATGCTGG 
     2101 CTCCGCCTCT GGGCAACACG CGGCTGCACG TGGTCAAGCT CCTGGCCAGT GCCCTGAGCG 
     2161 CCAATGATGC AGCCCTGACG CACGAGCTCC TGGCACTGGA CGTGCCCAAC ACCATGCTGG 
     2221 ACCTCTTCTT CCATTATGTC TTCAACAACT TCTTGCATGC CCAAGTAGAG GGATGCGTGA 
     2281 GCACCATGCT GAGCTTGGGG CCACCTCCTG ACAGCAGCCC TGAGACGCCC ATCCAAAACC 
     2341 CTGTTGTGAA ACATCTGCTG CAGCAGTGCC GCCTGGTGGA GCGGATCCTG ACGTCCTGGG 
     2401 AGGAGAACGA CCGTGTACAG TGTGCGGGAG GCCCTCGGAA AGGCTACATG GGTCACCTGA 
     2461 CAAGAGTGGC CGGTGCCCTG GTGCAGAACA CGGAGAAGGG GCCCAATGCA GAGCAGCTGC 
     2521 GGCAGCTGCT GAAGGAGCTG CCCAGCGAGC AGCAGGAGCA GTGGGAAGCC TTCGTATCGG 
     2581 GGCCCCTGGC GGAGACCAAC AAGAAGAACA TGGTGGACCT GGTGAACACC CACCACCTAC 
     2641 ACTCCTCCAG TGACGATGAG GACGACCGGC TCAAGGAGTT CAACTTCCCT GAGGAGGCTG 
     2701 TGCTGCAGCA GGCCTTCATG GACTTCCAGA TGCAGCGCAT GACCTCTGCC TTCATTGACC 
     2761 ACTTCGGCTT CAATGATGAG GAGTTTGGGG AGCAGGAGGA GAGTGTGAAC GCACCTTTTG 
     2821 ACAAGACAGC CAACATCACC TTCTCCCTCA ATGCTGACGA TGAGAACCCC AACGCCAACC 
     2881 TACTTGAGAT ATGCTACAAG GACCGCATCC AGCAGTTTGA TGATGATGAG GAAGAGGAGG 
     2941 ACGAGGAAGA GGCCCAGGGC TCAGGGGAGT CTGATGGAGA AGATGGCGCC TGGCAGGGCA 
     3001 GCCAGCTGGC CAGGGGGGCC CGTCTGGGCC AGCCACCTGG TGTCCGGAGT GGAGGCAGCA 
     3061 CAGACAGTGA GGACGAAGAA GAGGAGGACG AGGAGGAGGA GGAAGACGAG GAGGGCATTG 
     3121 GCTGTGCAGC CCGTGGAGGG GCCACCCCTC TGTCCTACCC CAGCCCTGGC CCTCAGCCTC 
     3181 CAGGCCCCAG CTGGACAGCC ACCTTTGACC CAGTGCCTAC AGATGCCCCG ACCAGCCCCC 
     3241 GAGTCTCCGG GGAGGAAGAG CTGCACACTG GGCCTCCAGC CCCACAGGGG CCCCTCAGTG 
     3301 TGCCCCAGGG CCTCCCCACT CAGAGCCTGG CCAGCCCTCC TGCCCGTGAC GCCCTGCAGC 
     3361 TCAGGTCTCA GGACCCCACA CCCCCCTCAG CACCTCAGGA AGCCACAGAA GGCAGCAAAG 
     3421 TCACGGAGCC CTCAGCCCCT TGCCAGGCCT TGGTTAGCAT CGGGGACCTT CAGGCCACCT 
     3481 TCCACGGGAT CCGTTCTGCC CCCAGCTCCT CGGACAGTGC AACCAGAGAC CCCTCTACCT 
     3541 CTGTCCCAGC CTCCGGGGCC CACCAGCCCC CCCAGACCAC AGAAGGGGAG AAGAGCCCAG 
     3601 AGCCCTTGGG GCTTCCCCAA AGCCAGAGTG CCCAGGCCCT CACGCCTCCT CCGATACCCA 
     3661 ATGGCTCTGC CCCGGAAGGG CCTGCATCCC CAGGCTCCCA AACGCGTACG CGGCCGCTCG 
     3721 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3781 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     3841 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     3901 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     3961 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     4021 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     4081 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     4141 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     4201 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4261 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4321 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4381 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4441 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4501 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4561 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4621 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4681 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4741 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4801 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4861 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4921 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4981 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5041 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5101 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5161 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     5221 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5281 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5341 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5401 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5461 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5521 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5581 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5641 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5701 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5761 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     5821 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     5881 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     5941 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     6001 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     6061 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     6121 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     6181 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6241 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6301 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6361 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6421 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6481 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6541 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6601 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6661 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6721 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6781 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     6841 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     6901 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     6961 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     7021 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     7081 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     7141 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     7201 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7261 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7321 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7381 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7441 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7501 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7561 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7621 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7681 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7741 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7801 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     7861 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     7921 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     7981 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     8041 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     8101 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     8161 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     8221 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8281 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8341 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8401 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8461 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8521 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8581 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8641 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8701 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8761 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     8821 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     8881 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     8941 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     9001 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     9061 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     9121 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     9181 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//