LOCUS       RG208913                8324 bp    DNA     circular  SYN 
DEFINITION  TCF7L1 (tGFP-tagged) - Human transcription factor 7-like 1 (T-cell specific, HMG-box) (TCF7L1)
ACCESSION   NM_031283
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2805..3509 
                     /label="GFP"  
     misc_feature    3556..4141 
                     /label="PolyA signal"  
     misc_feature    6233..6878 
                     /label="ColE1"  
     misc_feature    4595..5389 
                     /label="Neo"  
     misc_feature    4226..4528 
                     /label="SV40 ori"  
     misc_feature    6893..7753 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2784..2790 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2784 
                     /label="TCF7L1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCAGCTC GGCGGCGGGG GCGGCGGCGG CGGCGGCGGC AGCGGGGGAG 
     1081 GCGGCGGCTC CAGCGCCGGG GCGGCCGGCG GAGGGGACGA CCTCGGGGCG AACGACGAGC 
     1141 TGATCCCCTT CCAGGACGAG GGGGGCGAGG AGCAGGAGCC GAGCAGCGAT AGCGCCTCGG 
     1201 CGCAGCGGGA CCTAGACGAG GTCAAGTCGT CCCTGGTCAA CGAGTCGGAG AACCAGAGCA 
     1261 GCAGCTCGGA CTCGGAGGCG GAGAGGCGCC CGCAGCCCGT CCGGGACACT TTCCAGAAGC 
     1321 CGCGGGACTA TTTCGCCGAA GTGAGAAGGC CTCAGGACAG CGCGTTCTTT AAAGGACCCC 
     1381 CGTACCCTGG GTACCCCTTC CTGATGATCC CGGACCTGAG CAGCCCGTAC CTCTCCAACG 
     1441 GACCCCTGTC TCCCGGAGGA GCGCGCACCT ACCTGCAGAT GAAATGGCCC CTCCTCGATG 
     1501 TCCCCTCCAG CGCCACAGTC AAGGACACGA GGTCACCATC TCCAGCACAC TTGTCTAATA 
     1561 AAGTTCCTGT CGTTCAGCAC CCGCATCACA TGCATCCGCT GACTCCCCTC ATCACCTACA 
     1621 GCAATGACCA CTTCTCCCCC GGCTCCCCTC CCACCCACCT CTCCCCAGAG ATCGATCCAA 
     1681 AGACAGGAAT CCCCCGGCCC CCTCACCCAT CCGAGCTGTC ACCGTATTAC CCACTCTCTC 
     1741 CCGGAGCTGT CGGACAAATC CCCCACCCCC TCGGCTGGCT CGTCCCACAG CAAGGCCAGC 
     1801 CCATGTACTC CCTTCCTCCC GGTGGCTTCC GGCACCCTTA CCCCGCCCTC GCCATGAACG 
     1861 CCTCGATGTC CAGCCTGGTC TCCAGTCGGT TCTCTCCTCA CATGGTGGCT CCTGCCCACC 
     1921 CTGGCCTGCC CACCTCAGGG ATCCCCCACC CTGCCATCGT CTCCCCCATC GTCAAGCAGG 
     1981 AACCGGCACC CCCCAGCCTG AGCCCTGCAG TGAGCGTGAA ATCACCAGTC ACCGTGAAAA 
     2041 AGGAGGAGGA AAAGAAGCCC CACGTGAAGA AGCCTCTGAA TGCCTTCATG TTGTATATGA 
     2101 AGGAGATGAG GGCCAAGGTG GTGGCTGAGT GCACCCTGAA GGAAAGTGCA GCCATTAACC 
     2161 AGATCCTTGG AAGAAAGTGG CACAACCTGT CTCGAGAAGA ACAGGCCAAG TACTACGAGC 
     2221 TGGCCCGGAA GGAGCGGCAG CTTCACTCGC AGCTCTACCC AACCTGGTCA GCCCGGGACA 
     2281 ACTATGGTAA GAAAAAGAAG AGGAAGAGAG AAAAGCAGCT GTCCCAGACA CAGTCACAGC 
     2341 AGCAAGTCCA GGAGGCAGAG GGTGCCCTGG CCTCCAAGAG CAAGAAGCCA TGTGTTCAGT 
     2401 ACCTGCCCCC CGAGAAGCCC TGTGACAGCC CTGCCTCCTC CCACGGGAGC ATGCTGGACT 
     2461 CCCCGGCCAC TCCCTCTGCA GCTTTGGCCT CACCAGCTGC CCCTGCTGCC ACCCATTCGG 
     2521 AGCAAGCCCA GCCCCTCTCC CTCACCACCA AACCAGAAAC CCGGGCCCAG CTGGCTCTCC 
     2581 ACTCTGCCGC CTTCCTGTCG GCTAAGGCTG CAGCCTCCTC CTCTGGGCAG ATGGGCAGCC 
     2641 AGCCTCCCCT CCTGTCCCGG CCCCTCCCCC TTGGGTCCAT GCCCACAGCT CTGCTGGCCT 
     2701 CTCCCCCGTC CTTCCCCGCC ACGCTCCATG CCCACCAGGC CCTCCCGGTG CTACAGGCCC 
     2761 AGCCTCTTTC CCTGGTCACC AAGTCTGCCC ACACGCGTAC GCGGCCGCTC GAGATGGAGA 
     2821 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     2881 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     2941 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3001 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3061 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3121 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     3181 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     3241 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     3301 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     3361 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     3421 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     3481 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3541 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3601 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3661 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3721 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3781 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3841 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3901 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3961 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4021 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4081 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4141 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     4201 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     4261 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4321 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     4381 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     4441 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     4501 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     4561 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     4621 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     4681 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     4741 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     4801 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     4861 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     4921 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     4981 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5041 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     5101 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     5161 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     5221 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     5281 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     5341 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     5401 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     5461 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     5521 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     5581 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     5641 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     5701 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     5761 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     5821 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     5881 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     5941 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6001 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6061 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6121 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     6181 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     6241 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     6301 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     6361 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     6421 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     6481 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     6541 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     6601 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     6661 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     6721 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     6781 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     6841 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     6901 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     6961 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7021 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7081 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7141 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     7201 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     7261 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     7321 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     7381 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     7441 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     7501 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     7561 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     7621 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     7681 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     7741 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     7801 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     7861 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     7921 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     7981 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8041 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     8101 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     8161 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     8221 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     8281 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//