LOCUS       RG208825                9239 bp    DNA     circular  SYN 
DEFINITION  ARHGAP30 (tGFP-tagged) - Human Rho GTPase activating protein 30 (ARHGAP30), transcript variant 2
ACCESSION   NM_181720
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3720..4424 
                     /label="GFP"  
     misc_feature    4471..5056 
                     /label="PolyA signal"  
     misc_feature    7148..7793 
                     /label="ColE1"  
     misc_feature    5510..6304 
                     /label="Neo"  
     misc_feature    5141..5443 
                     /label="SV40 ori"  
     misc_feature    7808..8668 
                     /label="Amp"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3699..3705 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3699 
                     /label="ARHGAP30"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG AAGTCTCGGC AGAAAGGAAA GAAGAAGGGC AGCGCAAAGG 
     1081 AGCGGGTTTT TGGGTGCGAC TTGCAGGAGC ACCTGCAGCA CTCAGGCCAG GAGGTGCCCC 
     1141 AGGTGCTAAA GAGCTGTGCA GAATTTGTGG AGGAGTATGG AGTGGTGGAT GGGATCTACC 
     1201 GCCTCTCAGG GGTCTCCTCC AACATCCAGA AGCTTCGGCA GGAATTGGAG TCAGAGCGGA 
     1261 AGCCAGACCT GCGTCGGGAT GTTTACCTCC AAGACATTCA CTGCGTCTCC TCCCTGTGCA 
     1321 AGGCCTATTT CAGAGAACTG CCGGATCCCC TGCTCACTTA CCGGCTCTAT GACAAGTTTG 
     1381 CTGAGGCTGT AGGAGTGCAA TTGGAACCTG AGCGCTTGGT CAAGATCCTA GAGGTGCTTC 
     1441 GGGAACTCCC TGTCCCAAAC TACAGGACCC TGGAGTTCCT CATGAGGCAC TTGGTACACA 
     1501 TGGCCTCATT CAGTGCCCAG ACCAACATGC ATGCTCGCAA CCTGGCCATC GTGTGGGCTC 
     1561 CCAACCTGCT GAGGTCTAAG GACATAGAGG CCTCAGGCTT CAATGGGACA GCGGCCTTCA 
     1621 TGGAGGTGCG GGTACAATCC ATCGTCGTGG AGTTCATCCT CACACACGTG GACCAGCTCT 
     1681 TTGGGGGTGC TGCCCTCTCT GGTGGTGAGG TGGAGAGTGG GTGGCGATCG CTTCCAGGGA 
     1741 CCCGGGCATC AGGCAGCCCC GAGGACCTTA TGCCCAGGCC ACTGCCTTAT CACCTGCCTA 
     1801 GCATACTGCA GGCTGGCGAT GGACCCCCAC AGATGCGGCC CTACCATACT ATCATCGAGA 
     1861 TTGCAGAGCA CAAGAGGAAG GGGTCTTTGA AGGTCAGGAA GTGGAGGTCT ATCTTCAATT 
     1921 TAGGTCGCTC TGGCCATGAG ACTAAGCGTA AACTTCCACG GGGGGCTGAG GACAGGGAGG 
     1981 ATAAATCCAA CAAGGGGACA CTGCGGCCAG CCAAAAGCAT GGACTCACTG AGTGCTGCAG 
     2041 CTGGGGCCAG TGATGAGCCA GAGGGGCTGG TGGGGCCCAG CAGCCCCCGG CCAAGCCCAT 
     2101 TGCTGCCTGA GAGCTTGGAG AACGATTCTA TAGAGGCAGC AGAGGGTGAA CAGGAGCCTG 
     2161 AGGCAGAAGC ACTGGGTGGC ACAAACTCTG AACCAGGCAC ACCACGAGCT GGGCGGTCAG 
     2221 CCATCCGGGC TGGGGGCAGC AGCCGTGCAG AACGCTGTGC TGGTGTCCAC ATCTCAGACC 
     2281 CCTACAATGT CAACCTCCCG CTACACATCA CCTCTATCCT CAGTGTGCCC CCGAACATCA 
     2341 TCTCTAACGT TTCCTTGGCC AGGCTCACCC GTGGCCTTGA GTGCCCTGCT CTACAGCACC 
     2401 GGCCAAGCCC TGCCTCTGGC CCTGGCCCTG GCCCTGGCCT TGGCCCTGGC CCCCCAGATG 
     2461 AAAAGTTGGA AGCAAGTCCA GCCTCAAGTC CCCTGGCAGA CTCAGGCCCA GACGACTTGG 
     2521 CTCCTGCCCT GGAGGACTCG CTGTCCCAGG AGGTGCAGGA CTCCTTCTCC TTCCTAGAGG 
     2581 ACTCAAGCAG CTCAGAACCT GAGTGGGTGG GGGCAGAGGA TGGGGAGGTG GCCCAGGCAG 
     2641 AAGCAGCAGG AGCAGCCTTC TCCCCTGGGG AGGACGACCC TGGGATGGGC TACCTGGAGG 
     2701 AGCTCCTGGG AGTTGGGCCT CAGGTGGAGG AGTTCTCTGT GGAGCCACCC CTGGATGACC 
     2761 TGTCTCTGGA TGAGGCACAG TTTGTCTTGG CCCCCAGCTG CTGTTCCGTG GACTCCGCTG 
     2821 GCCCCAGGCC TGAAGTTGAG GAGGAAAATG GGGAGGAAGT TTTCCTGAGT GCCTATGATG 
     2881 ACCTAAGTCC CCTTCTGGGA CCTAAACCCC CAATCTGGAA GGGTTCAGGG AGTCTGGAGG 
     2941 GAGAGGCAGC AGGATGTGGA AGGCAGGCTC TGGGACAGGG TGGGGAAGAG CAGGCATGCT 
     3001 GGGAAGTTGG GGAGGACAAG CAGGCTGAGC CTGGAGGCAG GCTAGACATC AGGGAAGAGG 
     3061 CAGAGGGAAG TCCACAGCCA CCTCAGCCAG AGGAGATGGA GCCTGAGGGG CAGCCCAGTC 
     3121 CAGACGGCTG TCTATGCCCC TGTTCTCTTG GCCTGGGTGG CGTGGGCATG CGTCTAGCTT 
     3181 CCACTCTGGT TCAGGTCCAA CAGGTCCGCT CTGTGCCTGT GGTGCCCCCC AAGCCACAGT 
     3241 TTGCCAAGAT GCCCAGTGCA ATGTGTAGCA AGATTCATGT GGCACCTGCA AATCCATGCC 
     3301 CGAGGCCTGG CCGGCTTGAT GGGACTCCTG GAGAAAGGGC TTGGGGGTCC CGAGCTTCTC 
     3361 GATCCTCTTG GAGGAATGGG GGTAGTCTTT CCTTTGATGC TGCTGTGGCC CTAGCCCGGG 
     3421 ACCGCCAAAG GACTGAGGCT CAAGGAGTTC GGCGAACCCA GACCTGTACT GAGGGTGGGG 
     3481 ATTACTGCCT CATCCCCAGA ACCTCCCCTT GTAGCATGAT CTCTGCCCAT TCTCCTCGGC 
     3541 CCCTTAGCTG CCTGGAGCTC CCATCTGAAG GTGCAGAAGG GTCTGGATCC CGGAGTCGTC 
     3601 TTAGTCTGCC CCCCAGAGAA CCCCAGGTTC CTGACCCCCT GTTGTCCTCT CAGCGCAGAT 
     3661 CATATGCATT TGAAACACAG GCTAACCCTG GGAAAGGTGA AGGACTGACG CGTACGCGGC 
     3721 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3781 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3841 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3901 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     3961 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     4021 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4081 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4141 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4201 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4261 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4321 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4381 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4441 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4501 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4561 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4621 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4681 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4741 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4801 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4861 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4921 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4981 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5041 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5101 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5161 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5221 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5281 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5341 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5401 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5461 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5521 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5581 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5641 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5701 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5761 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5821 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5881 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     5941 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     6001 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6061 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6121 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6181 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6241 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6301 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6361 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6421 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6481 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6541 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6601 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6661 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6721 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6781 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6841 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6901 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     6961 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     7021 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7081 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7141 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7201 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7261 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7321 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7381 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7441 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7501 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7561 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7621 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7681 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7741 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7801 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7861 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7921 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     7981 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     8041 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8101 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8161 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8221 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8281 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8341 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8401 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8461 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8521 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8581 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8641 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8701 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8761 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8821 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8881 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     8941 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     9001 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9061 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9121 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9181 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//