LOCUS       RG208549                9032 bp    DNA     circular  SYN 
DEFINITION  NPAS2 (tGFP-tagged) - Human neuronal PAS domain protein 2 (NPAS2)
ACCESSION   NM_002518
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3513..4217 
                     /label="GFP"  
     misc_feature    4264..4849 
                     /label="PolyA signal"  
     misc_feature    6941..7586 
                     /label="ColE1"  
     misc_feature    5303..6097 
                     /label="Neo"  
     misc_feature    4934..5236 
                     /label="SV40 ori"  
     misc_feature    7601..8461 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3492..3498 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3492 
                     /label="NPAS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAAGAT GAGAAAGACA GAGCCAAGAG AGCTTCTCGA AACAAGTCTG 
     1081 AGAAGAAGCG TCGGGACCAG TTCAATGTTC TCATCAAAGA GCTCAGTTCC ATGCTCCCTG 
     1141 GCAACACGCG GAAAATGGAC AAAACCACCG TGTTGGAAAA GGTCATCGGA TTTTTGCAGA 
     1201 AACACAATGA AGTCTCAGCG CAAACGGAAA TCTGTGACAT TCAGCAAGAC TGGAAGCCTT 
     1261 CATTCCTCAG TAATGAAGAA TTCACCCAGC TGATGTTGGA GGCATTAGAT GGCTTCATTA 
     1321 TCGCAGTGAC AACAGACGGC AGCATCATCT ATGTCTCTGA CAGTATCACG CCTCTCCTTG 
     1381 GGCATTTACC GTCGGATGTC ATGGATCAGA ATTTGTTAAA TTTCCTCCCA GAACAAGAAC 
     1441 ATTCAGAAGT TTATAAAATC CTTTCTTCCC ATATGCTTGT GACGGATTCC CCCTCCCCAG 
     1501 AATACTTAAA ATCTGACAGC GATTTAGAGT TTTATTGCCA TCTTCTCAGA GGCAGCTTGA 
     1561 ACCCAAAGGA ATTTCCAACT TATGAATACA TAAAATTTGT AGGAAATTTT CGCTCTTACA 
     1621 ACAATGTGCC TAGCCCCTCC TGTAATGGTT TTGACAACAC CCTTTCAAGA CCTTGCCGGG 
     1681 TGCCACTAGG AAAGGAGGTT TGCTTCATTG CCACCGTTCG TCTGGCAACA CCACAATTCT 
     1741 TAAAGGAAAT GTGCATAGTT GACGAACCTT TAGAGGAATT CACTTCAAGG CATAGCTTGG 
     1801 AATGGAAATT TTTATTTCTG GATCACAGAG CACCTCCAAT CATAGGATAC CTGCCTTTTG 
     1861 AAGTGCTGGG AACCTCAGGC TATGACTACT ACCACATTGA TGACCTGGAG CTCCTGGCCA 
     1921 GGTGTCACCA GCACCTGATG CAGTTTGGCA AAGGGAAGTC GTGTTGCTAC CGGTTTCTGA 
     1981 CCAAAGGTCA GCAGTGGATC TGGCTGCAGA CTCACTACTA CATCACCTAC CATCAGTGGA 
     2041 ACTCCAAGCC CGAGTTCATC GTGTGCACAC ACTCGGTGGT CAGTTACGCA GATGTCCGGG 
     2101 TGGAAAGGAG GCAGGAGCTG GCTCTGGAAG ACCCGCCATC CGAGGCCCTC CACTCCTCAG 
     2161 CACTAAAGGA CAAGGGCTCA AGCCTGGAAC CTCGGCAGCA CTTTAACGCA CTCGACGTGG 
     2221 GTGCCTCGGG CCTTAATACC AGTCATTCGC CATCGGCGTC CTCAAGAAGT TCCCACAAAT 
     2281 CCTCGCACAC AGCCATGTCA GAACCCACCT CCACTCCCAC CAAGCTGATG GCAGAGGCCA 
     2341 GCACCCCGGC TTTGCCAAGA TCAGCCACCC TGCCCCAAGA GTTACCTGTC CCCGGGCTCA 
     2401 GCCAGGCAGC CACCATGCCG GCCCCTCTGC CTTCCCCATC GTCCTGCGAC CTCACACAGC 
     2461 AGCTCCTGCC TCAGACCGTT CTGCAGAGCA CGCCCGCTCC CATGGCACAG TTTTCGGCAC 
     2521 AGTTCAGCAT GTTCCAGACC ATCAAAGACC AGCTAGAGCA GCGGACGCGG ATCCTGCAGG 
     2581 CCAATATCCG GTGGCAACAG GAAGAGCTCC ACAAGATCCA GGAGCAGCTC TGCCTGGTCC 
     2641 AGGACTCCAA CGTCCAGATG TTCCTGCAGC AGCCAGCTGT ATCCCTGAGC TTCAGCAGCA 
     2701 CCCAGCGACC TGAGGCTCAG CAGCAGCTAC AGCAAAGGTC AGCTGCAGTG ACTCAGCCCC 
     2761 AGCTCGGGGC GGGCCCCCAA CTTCCAGGGC AGATCTCCTC TGCCCAGGTC ACAAGCCAGC 
     2821 ACCTGCTCAG AGAATCAAGT GTGATATCAA CCCAGGGTCC AAAGCCAATG AGAAGCTCAC 
     2881 AGCTAATGCA GAGCAGCGGC CGCTCTGGAA GCAGCCTAGT GTCCCCGTTC AGCAGCGCCA 
     2941 CAGCTGCGCT CCCGCCAAGT CTGAATCTGA CCACACCTGC TTCCACCTCC CAGGATGCCA 
     3001 GCCAGTGCCA GCCCAGCCCA GACTTCAGCC ATGATCGGCA GCTCAGGCTG TTGCTGAGCC 
     3061 AGCCCATCCA GCCCATGATG CCCGGGTCCT GTGACGCAAG GCAGCCCTCG GAAGTCAGCA 
     3121 GGACGGGACG GCAAGTCAAG TACGCCCAGA GCCAGACTGT GTTTCAAAAT CCAGACGCAC 
     3181 ACCCCGCCAA CAGCAGCAGC GCCCCGATGC CCGTCCTGCT GATGGGGCAG GCGGTGCTCC 
     3241 ACCCCAGCTT CCCTGCCTCC CAACCATCGC CCCTGCAGCC TGCACAGGCC CGGCAGCAGC 
     3301 CACCGCAGCA CTACCTGCAG GTACAGGCAC CAACCTCTTT GCACAGTGAG CAGCAGGACT 
     3361 CGCTACTTCT CTCCACCTAC TCACAACAGC CAGGGACCCT GGGCTACCCC CAACCACCCC 
     3421 CAGCACAGCC CCAGCCCCTA CGTCCTCCCC GAAGGGTCAG CAGTCTGTCT GAGTCGTCAG 
     3481 GCCTCCAGCA GCCGCCCCGA ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3541 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3601 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3661 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3721 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3781 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     3841 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     3901 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     3961 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4021 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4081 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4141 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4201 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4261 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4321 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4381 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4441 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4501 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4561 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4621 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4681 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4741 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4801 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4861 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     4921 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     4981 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5041 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5101 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5161 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5221 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5281 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5341 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5401 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5461 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5521 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5581 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5641 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5701 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5761 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     5821 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     5881 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     5941 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6001 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6061 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6121 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6181 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6241 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6301 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6361 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6421 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6481 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6541 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6601 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6661 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6721 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6781 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     6841 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     6901 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     6961 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7021 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7081 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7141 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7201 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7261 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7321 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7381 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7441 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7501 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7561 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7621 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7681 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7741 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7801 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     7861 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     7921 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     7981 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8041 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8101 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8161 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8221 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8281 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8341 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8401 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8461 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8521 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8581 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8641 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8701 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8761 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     8821 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     8881 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     8941 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9001 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//