LOCUS       RG208322                8786 bp    DNA     circular  SYN 
DEFINITION  SV2A (tGFP-tagged) - Human synaptic vesicle glycoprotein 2A (SV2A)
ACCESSION   NM_014849
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3267..3971 
                     /label="GFP"  
     misc_feature    4018..4603 
                     /label="PolyA signal"  
     misc_feature    6695..7340 
                     /label="ColE1"  
     misc_feature    5057..5851 
                     /label="Neo"  
     misc_feature    4688..4990 
                     /label="SV40 ori"  
     misc_feature    7355..8215 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3246..3252 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3246 
                     /label="SV2A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGAGGGC TTCCGAGACC GGGCAGCTTT CATCCGTGGG GCCAAAGACA 
     1081 TTGCTAAGGA AGTCAAAAAG CATGCGGCCA AGAAGGTGGT GAAGGGCCTG GACAGAGTCC 
     1141 AGGACGAATA TTCCCGAAGA TCGTACTCCC GCTTTGAGGA GGAGGATGAT GATGATGACT 
     1201 TCCCTGCTCC CAGTGATGGT TATTACCGAG GAGAAGGGAC CCAGGATGAG GAGGAAGGTG 
     1261 GTGCATCCAG TGATGCTACT GAGGGCCATG ACGAGGATGA TGAGATCTAT GAAGGGGAAT 
     1321 ATCAGGGCAT TCCCCGGGCA GAGTCTGGGG GCAAAGGCGA GCGGATGGCA GATGGGGCGC 
     1381 CCCTGGCTGG AGTAAGGGGG GGCTTGAGTG ATGGGGAGGG TCCCCCTGGG GGCCGGGGGG 
     1441 AGGCACAACG ACGGAAAGAA CGAGAAGAAC TGGCCCAACA GTATGAAGCC ATCCTACGGG 
     1501 AGTGTGGCCA CGGCCGCTTC CAGTGGACAC TGTATTTTGT GCTTGGTCTG GCGCTGATGG 
     1561 CTGACGGTGT GGAGGTCTTT GTGGTGGGCT TCGTGCTGCC CAGCGCTGAG AAAGACATGT 
     1621 GCCTGTCCGA CTCCAACAAA GGCATGCTAG GCCTCATCGT CTACCTGGGC ATGATGGTGG 
     1681 GAGCCTTCCT CTGGGGAGGT CTGGCTGACC GGCTGGGTCG GAGGCAGTGT CTGCTCATCT 
     1741 CGCTCTCAGT CAACAGCGTC TTCGCCTTCT TCTCATCTTT TGTCCAGGGT TACGGCACTT 
     1801 TCCTCTTCTG CCGCCTACTT TCTGGGGTTG GGATTGGAGG GTCCATCCCC ATTGTCTTCT 
     1861 CCTATTTCTC CGAGTTTCTG GCCCAGGAGA AACGAGGGGA GCATTTGAGC TGGCTCTGCA 
     1921 TGTTTTGGAT GATTGGTGGC GTGTACGCAG CTGCTATGGC CTGGGCCATC ATCCCCCACT 
     1981 ATGGGTGGAG TTTTCAGATG GGTTCTGCCT ACCAGTTCCA CAGCTGGAGG GTCTTCGTCC 
     2041 TCGTCTGCGC CTTTCCTTCT GTGTTTGCCA TTGGGGCTCT GACCACGCAG CCTGAGAGCC 
     2101 CCCGTTTCTT CCTAGAGAAT GGAAAGCATG ATGAGGCCTG GATGGTGCTG AAGCAGGTCC 
     2161 ATGATACCAA CATGCGAGCC AAAGGACATC CTGAGCGAGT GTTCTCAGTA ACCCACATTA 
     2221 AGACGATTCA TCAGGAGGAT GAATTGATTG AGATCCAGTC GGACACAGGG ACCTGGTACC 
     2281 AGCGCTGGGG GGTCCGGGCC TTGAGCCTAG GGGGGCAGGT TTGGGGGAAT TTTCTCTCCT 
     2341 GTTTTGGTCC CGAATATCGG CGCATCACTC TGATGATGAT GGGTGTGTGG TTCACCATGT 
     2401 CATTCAGCTA CTATGGCCTG ACCGTCTGGT TTCCTGACAT GATCCGCCAT CTCCAGGCAG 
     2461 TGGACTACGC ATCCCGCACC AAAGTGTTCC CCGGGGAGCG CGTAGAGCAT GTAACTTTTA 
     2521 ACTTCACGTT GGAGAATCAG ATCCACCGAG GCGGGCAGTA CTTCAATGAC AAGTTCATTG 
     2581 GGCTGCGGCT CAAGTCAGTG TCCTTTGAGG ATTCCCTGTT TGAAGAGTGT TATTTTGAGG 
     2641 ATGTCACATC CAGCAACACG TTTTTCCGCA ACTGCACATT CATCAACACT GTGTTCTATA 
     2701 ACACTGACCT GTTCGAGTAC AAGTTTGTGA ACAGCCGTCT GATAAACAGT ACATTCCTGC 
     2761 ACAACAAGGA GGGCTGCCCG CTAGACGTGA CAGGGACGGG CGAAGGTGCC TACATGGTAT 
     2821 ACTTTGTGAG CTTCCTGGGG ACACTGGCAG TGCTTCCTGG GAATATCGTG TCTGCCCTGC 
     2881 TCATGGACAA GATCGGCAGG CTCAGAATGC TTGCTGGCTC CAGCGTGATG TCCTGTGTCT 
     2941 CCTGCTTCTT CCTGTCTTTT GGGAACAGTG AGTCGGCCAT GATCGCTCTG CTCTGCCTTT 
     3001 TTGGCGGGGT CAGCATTGCA TCCTGGAATG CGCTGGACGT GTTGACTGTT GAACTCTACC 
     3061 CCTCAGACAA GAGGACCACA GCTTTTGGCT TCCTGAATGC CCTGTGTAAG CTGGCAGCTG 
     3121 TGCTGGGGAT CAGCATCTTC ACATCCTTCG TGGGAATCAC CAAGGCTGCA CCCATCCTCT 
     3181 TTGCCTCAGC TGCCCTTGCC CTTGGCAGCT CTCTGGCCCT GAAGCTGCCT GAGACCCGGG 
     3241 GGCAGGTGCT GCAGACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3301 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3361 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3421 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3481 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3541 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     3601 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     3661 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     3721 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     3781 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     3841 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     3901 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     3961 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4021 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4081 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4141 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4201 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4261 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4321 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4381 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4441 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4501 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4561 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4621 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     4681 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     4741 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4801 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     4861 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     4921 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     4981 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5041 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5101 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5161 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5221 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5281 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5341 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5401 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5461 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5521 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     5581 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     5641 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     5701 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     5761 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     5821 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     5881 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     5941 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6001 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6061 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6121 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6181 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6241 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6301 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6361 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6421 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6481 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6541 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     6601 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     6661 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     6721 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     6781 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     6841 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     6901 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     6961 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7021 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7081 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7141 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7201 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7261 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7321 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7381 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7441 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7501 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7561 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     7621 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     7681 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     7741 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     7801 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     7861 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     7921 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     7981 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8041 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8101 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8161 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8221 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8281 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8341 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8401 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8461 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8521 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     8581 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     8641 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     8701 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     8761 TTTAACAAAA ATTTAACGCG AATTTT
//