LOCUS       RG208033                9416 bp    DNA     circular  SYN 
DEFINITION  GAA (tGFP-tagged) - Human glucosidase, alpha; acid (GAA), transcript variant 3
ACCESSION   NM_001079804
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3897..4601 
                     /label="GFP"  
     misc_feature    4648..5233 
                     /label="PolyA signal"  
     misc_feature    7325..7970 
                     /label="ColE1"  
     misc_feature    5687..6481 
                     /label="Neo"  
     misc_feature    5318..5620 
                     /label="SV40 ori"  
     misc_feature    7985..8845 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3876..3882 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3876 
                     /label="GAA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGTGAGG CACCCGCCCT GCTCCCACCG GCTCCTGGCC GTCTGCGCCC 
     1081 TCGTGTCCTT GGCAACCGCT GCACTCCTGG GGCACATCCT ACTCCATGAT TTCCTGCTGG 
     1141 TTCCCCGAGA GCTGAGTGGC TCCTCCCCAG TCCTGGAGGA GACTCACCCA GCTCACCAGC 
     1201 AGGGAGCCAG TAGACCAGGG CCCCGGGATG CCCAGGCACA CCCCGGCCGT CCCAGAGCAG 
     1261 TGCCCACACA GTGCGACGTC CCCCCCAACA GCCGCTTCGA TTGCGCCCCT GACAAGGCCA 
     1321 TCACCCAGGA ACAGTGCGAG GCCCGCGGCT GTTGCTACAT CCCTGCAAAG CAGGGGCTGC 
     1381 AGGGAGCCCA GATGGGGCAG CCCTGGTGCT TCTTCCCACC CAGCTACCCC AGCTACAAGC 
     1441 TGGAGAACCT GAGCTCCTCT GAAATGGGCT ACACGGCCAC CCTGACCCGT ACCACCCCCA 
     1501 CCTTCTTCCC CAAGGACATC CTGACCCTGC GGCTGGACGT GATGATGGAG ACTGAGAACC 
     1561 GCCTCCACTT CACGATCAAA GATCCAGCTA ACAGGCGCTA CGAGGTGCCC TTGGAGACCC 
     1621 CGCATGTCCA CAGCCGGGCA CCGTCCCCAC TCTACAGCGT GGAGTTCTCC GAGGAGCCCT 
     1681 TCGGGGTGAT CGTGCGCCGG CAGCTGGACG GCCGCGTGCT GCTGAACACG ACGGTGGCGC 
     1741 CCCTGTTCTT TGCGGACCAG TTCCTTCAGC TGTCCACCTC GCTGCCCTCG CAGTATATCA 
     1801 CAGGCCTCGC CGAGCACCTC AGTCCCCTGA TGCTCAGCAC CAGCTGGACC AGGATCACCC 
     1861 TGTGGAACCG GGACCTTGCG CCCACGCCCG GTGCGAACCT CTACGGGTCT CACCCTTTCT 
     1921 ACCTGGCGCT GGAGGACGGC GGGTCGGCAC ACGGGGTGTT CCTGCTAAAC AGCAATGCCA 
     1981 TGGATGTGGT CCTGCAGCCG AGCCCTGCCC TTAGCTGGAG GTCGACAGGT GGGATCCTGG 
     2041 ATGTCTACAT CTTCCTGGGC CCAGAGCCCA AGAGCGTGGT GCAGCAGTAC CTGGACGTTG 
     2101 TGGGATACCC GTTCATGCCG CCATACTGGG GCCTGGGCTT CCACCTGTGC CGCTGGGGCT 
     2161 ACTCCTCCAC CGCTATCACC CGCCAGGTGG TGGAGAACAT GACCAGGGCC CACTTCCCCC 
     2221 TGGACGTCCA GTGGAACGAC CTGGACTACA TGGACTCCCG GAGGGACTTC ACGTTCAACA 
     2281 AGGATGGCTT CCGGGACTTC CCGGCCATGG TGCAGGAGCT GCACCAGGGC GGCCGGCGCT 
     2341 ACATGATGAT CGTGGATCCT GCCATCAGCA GCTCGGGCCC TGCCGGGAGC TACAGGCCCT 
     2401 ACGACGAGGG TCTGCGGAGG GGGGTTTTCA TCACCAACGA GACCGGCCAG CCGCTGATTG 
     2461 GGAAGGTATG GCCCGGGTCC ACTGCCTTCC CCGACTTCAC CAACCCCACA GCCCTGGCCT 
     2521 GGTGGGAGGA CATGGTGGCT GAGTTCCATG ACCAGGTGCC CTTCGACGGC ATGTGGATTG 
     2581 ACATGAACGA GCCTTCCAAC TTCATCAGGG GCTCTGAGGA CGGCTGCCCC AACAATGAGC 
     2641 TGGAGAACCC ACCCTACGTG CCTGGGGTGG TTGGGGGGAC CCTCCAGGCG GCCACCATCT 
     2701 GTGCCTCCAG CCACCAGTTT CTCTCCACAC ACTACAACCT GCACAACCTC TACGGCCTGA 
     2761 CCGAAGCCAT CGCCTCCCAC AGGGCGCTGG TGAAGGCTCG GGGGACACGC CCATTTGTGA 
     2821 TCTCCCGCTC GACCTTTGCT GGCCACGGCC GATACGCCGG CCACTGGACG GGGGACGTGT 
     2881 GGAGCTCCTG GGAGCAGCTC GCCTCCTCCG TGCCAGAAAT CCTGCAGTTT AACCTGCTGG 
     2941 GGGTGCCTCT GGTCGGGGCC GACGTCTGCG GCTTCCTGGG CAACACCTCA GAGGAGCTGT 
     3001 GTGTGCGCTG GACCCAGCTG GGGGCCTTCT ACCCCTTCAT GCGGAACCAC AACAGCCTGC 
     3061 TCAGTCTGCC CCAGGAGCCG TACAGCTTCA GCGAGCCGGC CCAGCAGGCC ATGAGGAAGG 
     3121 CCCTCACCCT GCGCTACGCA CTCCTCCCCC ACCTCTACAC ACTGTTCCAC CAGGCCCACG 
     3181 TCGCGGGGGA GACCGTGGCC CGGCCCCTCT TCCTGGAGTT CCCCAAGGAC TCTAGCACCT 
     3241 GGACTGTGGA CCACCAGCTC CTGTGGGGGG AGGCCCTGCT CATCACCCCA GTGCTCCAGG 
     3301 CCGGGAAGGC CGAAGTGACT GGCTACTTCC CCTTGGGCAC ATGGTACGAC CTGCAGACGG 
     3361 TGCCAGTAGA GGCCCTTGGC AGCCTCCCAC CCCCACCTGC AGCTCCCCGT GAGCCAGCCA 
     3421 TCCACAGCGA GGGGCAGTGG GTGACGCTGC CGGCCCCCCT GGACACCATC AACGTCCACC 
     3481 TCCGGGCTGG GTACATCATC CCCCTGCAGG GCCCTGGCCT CACAACCACA GAGTCCCGCC 
     3541 AGCAGCCCAT GGCCCTGGCT GTGGCCCTGA CCAAGGGTGG GGAGGCCCGA GGGGAGCTTT 
     3601 TCTGGGACGA TGGAGAGAGC CTGGAAGTGC TGGAGCGAGG GGCCTACACA CAGGTCATCT 
     3661 TCCTGGCCAG GAATAACACG ATCGTGAATG AGCTGGTACG TGTGACCAGT GAGGGAGCTG 
     3721 GCCTGCAGCT GCAGAAGGTG ACTGTCCTGG GCGTGGCCAC GGCGCCCCAG CAGGTCCTCT 
     3781 CCAACGGTGT CCCTGTCTCC AACTTCACCT ACAGCCCCGA CACCAAGGTC CTGGACATCT 
     3841 GTGTCTCGCT GTTGATGGGA GAGCAGTTTC TCGTCAGCTG GTGTACGCGT ACGCGGCCGC 
     3901 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3961 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     4021 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     4081 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     4141 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     4201 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     4261 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4321 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4381 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4441 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4501 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4561 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4621 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4681 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4741 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4801 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4861 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4921 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4981 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5041 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5101 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5161 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5221 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5281 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5341 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5401 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5461 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5521 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5581 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5641 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5701 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5761 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5821 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5881 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5941 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     6001 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     6061 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     6121 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     6181 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     6241 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6301 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6361 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6421 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6481 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6541 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6601 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6661 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6721 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6781 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6841 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6901 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6961 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     7021 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     7081 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     7141 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     7201 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     7261 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7321 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7381 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7441 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7501 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7561 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7621 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7681 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7741 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7801 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7861 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7921 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7981 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     8041 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     8101 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     8161 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     8221 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     8281 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8341 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8401 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8461 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8521 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8581 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8641 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8701 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8761 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8821 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8881 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8941 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     9001 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     9061 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     9121 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     9181 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     9241 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9301 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9361 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//