LOCUS       RG207886                9320 bp    DNA     circular  SYN 
DEFINITION  NUP98 (GFP-tagged) - Human nucleoporin 98kDa (NUP98), transcript variant 2
ACCESSION   NM_139131
VERSION     NM_139131.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3801..4505 
                     /label="GFP"  
     misc_feature    4552..5137 
                     /label="PolyA signal"  
     misc_feature    7229..7874 
                     /label="ColE1"  
     misc_feature    5591..6385 
                     /label="Neo"  
     misc_feature    5222..5524 
                     /label="SV40 ori"  
     misc_feature    7889..8749 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3780..3786 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3780 
                     /label="NUP98"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTAACAAA TCATTTGGAA CACCCTTTGG GGGTGGCACA GGTGGCTTTG 
     1081 GCACAACTTC AACATTTGGA CAGAATACTG GCTTTGGCAC TACTAGTGGA GGGGCATTTG 
     1141 GAACATCTGC ATTTGGTTCT AGCAACAATA CTGGAGGCCT CTTTGGAAAT TCACAGACTA 
     1201 AACCAGGAGG ATTGTTTGGA ACCAGTTCAT TTAGCCAGCC AGCTACCTCC ACAAGCACTG 
     1261 GCTTTGGGTT TGGTACGTCA ACAGGAACAG CAAATACCTT GTTTGGAACT GCAAGCACAG 
     1321 GGACCAGTCT CTTCTCATCC CAAAACAATG CCTTTGCACA AAATAAACCA ACTGGCTTTG 
     1381 GCAATTTTGG AACCAGTACT AGCAGTGGAG GACTCTTTGG AACCACAAAT ACCACCTCTA 
     1441 ATCCTTTTGG CAGCACATCT GGCTCCCTCT TTGGGCCAAG TAGTTTTACA GCTGCTCCTA 
     1501 CTGGGACTAC TATTAAATTT AACCCTCCAA CTGGTACAGA TACTATGGTC AAAGCTGGAG 
     1561 TTAGCACTAA CATAAGTACC AAGCACCAGT GTATTACTGC TATGAAAGAA TATGAAAGCA 
     1621 AGTCACTAGA GGAACTTCGT TTAGAGGATT ATCAGGCTAA CAGGAAGGGC CCACAGAACC 
     1681 AGGTGGGAGC AGGTACCACA ACTGGCTTGT TTGGGTCTTC TCCAGCCACT TCCAGCGCAA 
     1741 CAGGACTCTT CAGCTCCTCC ACCACTAATT CAGGCTTTGC ATATGGTCAG AACAAAACTG 
     1801 CCTTTGGAAC TAGTACAACT GGATTTGGAA CAAATCCAGG TGGTCTCTTT GGCCAACAGA 
     1861 ATCAGCAGAC TACCAGCCTC TTCAGCAAAC CATTTGGCCA GGCTACAACC ACCCAGAACA 
     1921 CTGGCTTTTC CTTTGGTAAT ACCAGCACCA TAGGACAGCC AAGCACCAAC ACCATGGGAT 
     1981 CATTTGGAGT AACCCAAGCC TCACAGCCTG GAGGTCTTTT TGGGACAGCT ACAAACACCA 
     2041 GCACTGGGAC AGCATTTGGA ACAGGAACAG GTCTCTTTGG GCAGACCAAT ACTGGATTTG 
     2101 GTGCTGTTGG TTCGACCCTG TTTGGCAATA ACAAGCTTAC TACATTTGGA AGCGGCACAA 
     2161 CCAGTGCACC TTCATTTGGT ACAACCAGTG GCGGGCTCTT TGGTTTTGGC ACAAATACCA 
     2221 GTGGGAATAG TATTTTTGGA AGTAAACCAG CACCTGGGAC TCTTGGAACT GGGCTTGGTG 
     2281 CAGGATTTGG AACAGCTCTT GGTGCTGGAC AGGCATCTTT GTTTGGGAAC AACCAACCTA 
     2341 AGATTGGAGG GCCTCTTGGT ACAGGAGCCT TTGGGGCCCC TGGATTTAAT ACTACGACAG 
     2401 CCACTTTGGG CTTTGGAGCC CCCCAGGCCC CAGTAGCTTT GACAGATCCA AATGCTTCTG 
     2461 CTGCCCAGCA GGCTGTTCTC CAGCAGCACA TCAATAGTCT AACATACTCA CCTTTTGGAG 
     2521 ACTCTCCTCT CTTCCGGAAT CCGATGTCAG ACCCTAAGAA GAAGGAAGAG AGATTGAAAC 
     2581 CAACAAATCC AGCAGCCCAG AAGGCTCTTA CTACACCTAC TCATTATAAA CTGACACCCC 
     2641 GCCCTGCCAC TAGAGTCCGG CCAAAGGCTT TACAAACAAC AGGCACAGCC AAGTCACATC 
     2701 TCTTTGATGG GCTGGATGAC GATGAACCAT CCCTAGCCAA TGGAGCATTC ATGCCCAAGA 
     2761 AGAGCATTAA GAAGTTGGTT TTGAAGAACC TTAATAATAG CAATCTCTTT TCTCCTGTTA 
     2821 ATCGTGATTC AGAAAATCTA GCTTCACCAT CTGAATATCC AGAAAATGGA GAGAGATTTA 
     2881 GTTTCCTAAG CAAACCTGTT GATGAGAATC ACCAGCAGGA TGGAGATGAA GATTCCCTTG 
     2941 TTTCACATTT TTATACTAAC CCTATTGCCA AACCTATTCC TCAAACCCCA GAAAGTGCTG 
     3001 GAAATAAACA CAGCAACAGC AACAGTGTGG ATGATACCAT TGTTGCATTA AACATGCGTG 
     3061 CTGCTTTGCG AAATGGGCTG GAAGGAAGCA GTGAAGAAAC GTCTTTTCAT GATGAGTCAC 
     3121 TTCAGGATGA CCGAGAAGAA ATAGAAAATA ATTCTTACCA TATGCACCCA GCAGGTATTA 
     3181 TTCTCACTAA GGTTGGTTAC TATACTATTC CATCTATGGA TGACCTTGCT AAAATTACCA 
     3241 ATGAAAAAGG AGAGTGCATT GTCTCTGATT TCACTATTGG TCGGAAAGGT TATGGTTCAA 
     3301 TCTATTTTGA AGGAGATGTG AATTTGACAA ATCTAAATTT GGATGATATT GTGCATATCC 
     3361 GGAGGAAAGA AGTAGTTGTC TACTTAGATG ATAACCAAAA ACCACCTGTG GGTGAAGGGC 
     3421 TAAATAGGAA GGCTGAAGTT ACATTGGATG GAGTTTGGCC AACAGATAAA ACATCTCGTT 
     3481 GTTTAATAAA GAGCCCAGAT CGCCTTGCTG ATATCAACTA TGAAGGAAGA TTGGAAGCAG 
     3541 TTTCAAGGAA ACAGGGAGCT CAATTCAAAG AATACCGGCC TGAAACTGGT TCTTGGGTGT 
     3601 TTAAGGTCTC CCATTTTTCT AAGTATGGCC TTCAGGATTC TGATGAAGAG GAGGAGGAGC 
     3661 ATCCGTCTAA AACTAGTACA AAGAAGTTGA AGACTGCTCC TTTGCCTCCT GCAAGCCAGA 
     3721 CTACGCCCTT GCAGATGGCT CTTAATGGCA AACCTGCACC TCCACCTCAG GTAGAGAAAA 
     3781 AAGGACAGAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3841 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3901 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3961 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     4021 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     4081 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     4141 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4201 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4261 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4321 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4381 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4441 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4501 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5221 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5281 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5341 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5401 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5461 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5521 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5581 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5641 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5701 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5761 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5821 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5881 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5941 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     6001 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     6061 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     6121 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6181 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6241 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6301 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6361 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6421 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6481 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6541 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6601 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6661 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6721 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6781 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6841 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6901 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6961 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     7021 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     7081 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     7141 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7201 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7261 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7321 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7381 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7441 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7501 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7561 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7621 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7681 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7741 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7801 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7861 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7921 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7981 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     8041 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     8101 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     8161 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8221 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8281 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8341 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8401 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8461 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8521 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8581 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8641 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8701 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8761 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8821 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8881 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8941 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     9001 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     9061 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     9121 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9181 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9241 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9301 AAAAATTTAA CGCGAATTTT 
//