LOCUS       RG207192                9281 bp    DNA     circular  SYN 
DEFINITION  FIG4 (tGFP-tagged) - Human FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae) (FIG4)
ACCESSION   NM_014845
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3762..4466 
                     /label="GFP"  
     misc_feature    4513..5098 
                     /label="PolyA signal"  
     misc_feature    7190..7835 
                     /label="ColE1"  
     misc_feature    5552..6346 
                     /label="Neo"  
     misc_feature    5183..5485 
                     /label="SV40 ori"  
     misc_feature    7850..8710 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3741..3747 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3741 
                     /label="FIG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCACGGCC GCCGCCCCCA TCATCAGCTC GGTCCAGAAG CTGGTTCTGT 
     1081 ATGAGACTAG AGCTAGATAC TTTCTAGTTG GGAGCAATAA TGCAGAAACG AAATATCGTG 
     1141 TCTTGAAGAT TGATAGAACA GAACCAAAAG ATTTGGTCAT AATTGATGAC AGGCATGTCT 
     1201 ATACTCAACA AGAAGTAAGG GAACTTCTTG GCCGCTTGGA TCTTGGAAAT AGAACAAAGA 
     1261 TGGGACAGAA AGGATCCTCG GGCTTATTTC GAGCGGTTTC AGCTTTTGGT GTTGTGGGTT 
     1321 TTGTCAGGTT CTTAGAAGGC TATTATATTG TGTTAATAAC TAAAAGGAGG AAGATGGCGG 
     1381 ATATTGGAGG TCATGCAATC TATAAGGTCG AAGATACAAA TATGATCTAT ATACCCAATG 
     1441 ATTCTGTACG GGTTACTCAT CCTGATGAAG CTAGGTATCT ACGAATATTT CAAAATGTGG 
     1501 ACCTATCTAG CAATTTTTAC TTTAGTTACA GCTATGATTT GTCCCACTCA CTTCAATATA 
     1561 ATCTCACTGT CTTGCGAATG CCCCTGGAGA TGTTAAAGTC AGAAATGACC CAGAATCGCC 
     1621 AAGAGAGCTT TGACATCTTT GAAGATGAAG GATTAATTAC ACAAGGTGGA AGCGGGGTAT 
     1681 TTGGGATCTG TAGTGAGCCT TATATGAAAT ATGTATGGAA TGGTGAACTT CTGGATATAA 
     1741 TTAAAAGTAC TGTGCATCGT GACTGGCTTT TGTATATTAT TCATGGGTTC TGTGGGCAGT 
     1801 CAAAGCTGTT GATCTATGGA CGACCAGTGT ATGTCACTCT AATAGCTAGA AGATCCAGTA 
     1861 AATTTGCTGG CACCCGTTTT CTTAAAAGAG GTGCAAACTG TGAGGGTGAT GTTGCAAATG 
     1921 AAGTGGAGAC TGAACAAATA CTCTGCGATG CTTCTGTGAT GTCTTTCACT GCAGGAAGTT 
     1981 ATTCTTCATA TGTACAAGTT AGAGGATCTG TGCCCTTATA CTGGTCTCAG GACATTTCAA 
     2041 CTATGATGCC TAAACCACCT ATTACATTGG ATCAGGCAGA TCCATTTGCA CATGTGGCTG 
     2101 CCCTTCACTT TGACCAGATG TTCCAGAGGT TTGGCTCTCC CATCATCATC TTGAATTTAG 
     2161 TGAAGGAACG AGAGAAAAGA AAGCATGAAA GAATTCTGAG TGAAGAACTT GTTGCTGCTG 
     2221 TGACCTATCT CAACCAATTT TTGCCTCCTG AGCACACTAT TGTTTATATT CCCTGGGACA 
     2281 TGGCCAAGTA TACCAAAAGC AAGCTGTGTA ATGTTCTTGA TCGACTAAAT GTGATTGCAG 
     2341 AAAGTGTGGT GAAGAAAACA GGTTTCTTTG TAAACCGCCC TGATTCTTAC TGTAGCATTT 
     2401 TGCGGCCAGA TGAAAAGTGG AATGAACTAG GAGGATGTGT GATTCCCACT GGTCGCCTGC 
     2461 AGACTGGCAT CCTTCGAACC AACTGTGTGG ACTGTTTAGA TCGCACCAAC ACAGCACAGT 
     2521 TTATGGTGGG AAAATGTGCT CTGGCCTATC AGCTGTATTC ACTGGGACTG ATTGACAAAC 
     2581 CTAATCTACA GTTTGATACA GATGCAGTTA GGTTATTTGA GGAACTCTAT GAAGATCATG 
     2641 GTGATACCCT ATCCCTTCAG TATGGTGGTT CTCAACTTGT TCATCGTGTG AAAACCTACA 
     2701 GAAAGATAGC ACCATGGACC CAGCACTCCA AAGACATCAT GCAAACCCTG TCTAGATATT 
     2761 ACAGCAATGC TTTTTCAGAT GCCGATAGAC AAGATTCCAT TAATCTCTTC CTGGGAGTTT 
     2821 TCCATCCCAC TGAAGGGAAA CCTCATCTCT GGGAGCTCCC AACAGATTTT TATTTGCATC 
     2881 ACAAAAATAC CATGAGACTT TTGCCAACAA GAAGAAGTTA TACTTACTGG TGGACACCAG 
     2941 AGGTGATAAA GCATTTACCA TTGCCCTATG ATGAAGTTAT CTGTGCTGTG AACTTAAAGA 
     3001 AGTTGATAGT GAAGAAATTC CACAAATATG AAGAAGAGAT TGATATCCAC AATGAGTTCT 
     3061 TTCGGCCATA TGAGTTGAGC AGCTTTGATG ATACCTTTTG CTTGGCTATG ACAAGCTCAG 
     3121 CACGTGACTT TATGCCTAAG ACCGTTGGAA TTGATCCAAG TCCATTTACT GTGCGTAAAC 
     3181 CAGATGAAAC TGGAAAATCA GTATTGGGAA ACAAAAGCAA TAGAGAAGAA GCTGTATTAC 
     3241 AGCGGAAAAC GGCAGCCAGC GCCCCGCCGC CACCCAGCGA GGAGGCTGTG TCCAGCAGCT 
     3301 CTGAGGATGA CTCTGGGACT GATCGGGAAG AAGAGGGCTC TGTGTCTCAG CGCTCCACTC 
     3361 CCGTGAAGAT GACTGATGCA GGAGACAGTG CCAAAGTGAC CGAGAATGTG GTCCAACCCA 
     3421 TGAAGGAGCT ATATGGAATT AACCTCTCAG ATGGCCTCTC AGAAGAAGAT TTCTCCATTT 
     3481 ATTCAAGATT TGTTCAGCTG GGGCAGAGTC AACATAAACA AGACAAGAAT AGCCAGCAGC 
     3541 CCTGTTCTAG GTGCTCAGAT GGAGTTATAA AACTAACACC CATCTCGGCT TTCTCGCAAG 
     3601 ATAACATCTA TGAAGTTCAG CCCCCAAGAG TAGACAGAAA ATCTACAGAG ATCTTCCAAG 
     3661 CCCACATCCA GGCCAGCCAA GGTATCATGC AGCCCCTAGG AAAAGAGGAC TCCTCCATGT 
     3721 ACCGAGAGTA CATCAGGAAC CGCTACCTGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3781 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3841 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3901 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3961 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     4021 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4081 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4141 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4201 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4261 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4321 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4381 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4441 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4501 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4561 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4621 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4681 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4741 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4801 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4861 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4921 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4981 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5041 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5101 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5161 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5221 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5281 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5341 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5401 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5461 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5521 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5581 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5641 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5701 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5761 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5821 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5881 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5941 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     6001 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6061 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6121 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6181 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6241 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6301 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6361 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6421 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6481 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6541 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6601 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6661 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6721 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6781 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6841 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6901 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6961 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     7021 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7081 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7141 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7201 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7261 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7321 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7381 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7441 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7501 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7561 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7621 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7681 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7741 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7801 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7861 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7921 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7981 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     8041 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8101 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8161 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8221 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8281 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8341 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8401 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8461 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8521 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8581 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8641 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8701 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8761 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8821 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8881 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8941 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     9001 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9061 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9121 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9181 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9241 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//