LOCUS       RG206820                8276 bp    DNA     circular  SYN 
DEFINITION  ME1 (tGFP-tagged) - Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1)
ACCESSION   NM_002395
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2757..3461 
                     /label="GFP"  
     misc_feature    3508..4093 
                     /label="PolyA signal"  
     misc_feature    6185..6830 
                     /label="ColE1"  
     misc_feature    4547..5341 
                     /label="Neo"  
     misc_feature    4178..4480 
                     /label="SV40 ori"  
     misc_feature    6845..7705 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2736..2742 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2736 
                     /label="ME1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCGAA GCCCCCCGTC GCCGCCACAC CCATCAGCGC GGCTACCTGC 
     1081 TGACACGGAA CCCTCACCTC AACAAGGACT TGGCCTTTAC CCTGGAAGAG AGACAGCAAT 
     1141 TGAACATTCA TGGATTGTTG CCACCTTCCT TCAACAGTCA GGAGATCCAG GTTCTTAGAG 
     1201 TAGTAAAAAA TTTCGAGCAT CTGAACTCTG ACTTTGACAG GTATCTTCTC TTAATGGATC 
     1261 TCCAAGATAG AAATGAAAAA CTCTTTTATA GAGTGCTGAC ATCTGACATT GAGAAATTCA 
     1321 TGCCTATTGT TTATACTCCC ACTGTGGGTC TGGCTTGCCA ACAATATAGT TTGGTGTTTC 
     1381 GGAAGCCAAG AGGTCTCTTT ATTACTATCC ACGATCGAGG GCATATTGCT TCAGTTCTCA 
     1441 ATGCATGGCC AGAAGATGTC ATCAAGGCCA TTGTGGTGAC TGATGGAGAG CGTATTCTTG 
     1501 GCTTGGGAGA CCTTGGCTGT AATGGAATGG GCATCCCTGT GGGTAAATTG GCTCTATATA 
     1561 CAGCTTGCGG AGGGATGAAT CCTCAAGAAT GTCTGCCTGT CATTCTGGAT GTGGGAACCG 
     1621 AAAATGAGGA GTTACTTAAA GATCCACTCT ACATTGGACT ACGGCAGAGA AGAGTAAGAG 
     1681 GTTCTGAATA TGATGATTTT TTGGACGAAT TCATGGAGGC AGTTTCTTCC AAGTATGGCA 
     1741 TGAATTGCCT TATTCAGTTT GAAGATTTTG CCAATGTGAA TGCATTTCGT CTCCTGAACA 
     1801 AGTATCGAAA CCAGTATTGC ACATTCAATG ATGATATTCA AGGAACAGCA TCTGTTGCAG 
     1861 TTGCAGGTCT CCTTGCAGCT CTTCGAATAA CCAAGAACAA ACTGTCTGAT CAAACAATAC 
     1921 TATTCCAAGG AGCTGGAGAG GCTGCCCTAG GGATTGCACA CCTGATTGTG ATGGCCTTGG 
     1981 AAAAAGAAGG TTTACCAAAA GAGAAAGCCA TCAAAAAGAT ATGGCTGGTT GATTCAAAAG 
     2041 GATTAATAGT TAAGGGACGT GCTTCCTTAA CACAAGAGAA AGAGAAGTTT GCCCATGAAC 
     2101 ATGAAGAAAT GAAGAACCTA GAAGCCATTG TTCAAGAAAT AAAACCAACT GCCCTCATAG 
     2161 GAGTTGCTGC AATTGGTGGT GCATTCTCAG AACAAATTCT CAAAGATATG GCTGCCTTCA 
     2221 ATGAACGGCC TATTATTTTT GCTTTGAGTA ATCCAACTAG CAAAGCAGAA TGTTCTGCAG 
     2281 AGCAGTGCTA CAAAATAACC AAGGGACGTG CAATTTTTGC CAGTGGCAGT CCTTTTGATC 
     2341 CAGTCACTCT TCCAAATGGA CAGACCCTAT ATCCTGGCCA AGGCAACAAT TCCTATGTGT 
     2401 TCCCTGGAGT TGCTCTTGGT GTTGTGGCGT GTGGATTGAG GCAGATCACA GATAATATTT 
     2461 TCCTCACTAC TGCTGAGGTT ATAGCTCAGC AAGTGTCAGA TAAACACTTG GAAGAGGGTC 
     2521 GGCTTTATCC TCCTTTGAAT ACCATTAGAG ATGTTTCTCT GAAAATTGCA GAAAAGATTG 
     2581 TGAAAGATGC ATACCAAGAA AAGACAGCCA CAGTTTATCC TGAACCGCAA AACAAAGAAG 
     2641 CATTTGTCCG CTCCCAGATG TATAGTACTG ATTATGACCA GATTCTACCT GATTGTTATT 
     2701 CTTGGCCTGA AGAGGTGCAG AAAATACAGA CCAAAGTTGA CCAGACGCGT ACGCGGCCGC 
     2761 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     2821 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     2881 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     2941 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3001 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3061 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     3121 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     3181 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     3241 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     3301 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     3361 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     3421 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     3481 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3541 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3601 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3661 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3721 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3781 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3841 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3901 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3961 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4021 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4081 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4141 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4201 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     4261 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     4321 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     4381 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     4441 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     4501 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     4561 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     4621 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     4681 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     4741 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     4801 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     4861 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     4921 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     4981 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5041 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     5101 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     5161 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     5221 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     5281 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     5341 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     5401 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     5461 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     5521 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     5581 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     5641 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     5701 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     5761 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     5821 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     5881 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     5941 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6001 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6061 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     6121 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     6181 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     6241 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     6301 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     6361 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     6421 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     6481 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     6541 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     6601 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     6661 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     6721 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     6781 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     6841 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     6901 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     6961 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7021 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7081 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     7141 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     7201 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     7261 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     7321 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     7381 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     7441 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     7501 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     7561 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     7621 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     7681 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     7741 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     7801 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     7861 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     7921 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     7981 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8041 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8101 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     8161 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     8221 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//