LOCUS       RG206408                9026 bp    DNA     circular  SYN 
DEFINITION  FES (tGFP-tagged) - Human feline sarcoma oncogene (FES), transcript variant 1
ACCESSION   NM_002005
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3507..4211 
                     /label="GFP"  
     misc_feature    4258..4843 
                     /label="PolyA signal"  
     misc_feature    6935..7580 
                     /label="ColE1"  
     misc_feature    5297..6091 
                     /label="Neo"  
     misc_feature    4928..5230 
                     /label="SV40 ori"  
     misc_feature    7595..8455 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3486..3492 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3486 
                     /label="FES"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTTCTCT TCTGAGCTGT GCAGCCCCCA GGGCCACGGG GTCCTGCAGC 
     1081 AAATGCAGGA GGCCGAGCTT CGTCTACTGG AGGGCATGAG AAAGTGGATG GCCCAGCGGG 
     1141 TCAAGAGTGA CAGGGAGTAT GCAGGACTGC TTCACCACAT GTCCCTGCAG GACAGTGGGG 
     1201 GCCAGAGCCG GGCCATCAGC CCTGACAGCC CCATCAGTCA GTCCTGGGCT GAGATCACCA 
     1261 GCCAAACTGA GGGCCTGAGC CGCTTGCTGC GGCAGCACGC AGAGGATCTG AACTCAGGGC 
     1321 CCCTGAGCAA GCTGAGCCTG CTCATCCGGG AACGGCAGCA GCTTCGCAAG ACCTACAGCG 
     1381 AGCAGTGGCA GCAGCTGCAG CAGGAGCTCA CCAAGACCCA CAGCCAGGAC ATTGAGAAGC 
     1441 TGAAGAGCCA GTACCGAGCT CTGGCACGGG ACAGTGCCCA AGCCAAGCGC AAGTACCAGG 
     1501 AGGCCAGCAA AGACAAGGAC CGTGACAAGG CCAAGGACAA GTATGTGCGC AGCCTGTGGA 
     1561 AGCTCTTTGC TCACCACAAC CGCTATGTGC TGGGCGTGCG GGCTGCGCAG CTACACCACC 
     1621 AGCACCACCA CCAGCTCCTG CTGCCCGGCC TGCTGCGGTC ACTGCAGGAC CTGCACGAGG 
     1681 AGATGGCTTG CATCCTGAAG GAGATCCTGC AGGAATACCT GGAGATTAGC AGCCTGGTGC 
     1741 AGGATGAGGT GGTGGCCATT CACCGGGAGA TGGCTGCAGC TGCTGCCCGC ATCCAGCCTG 
     1801 AGGCTGAGTA CCAAGGCTTC CTGCGACAGT ATGGGTCCGC ACCTGACGTC CCACCCTGTG 
     1861 TCACGTTCGA TGAGTCACTG CTTGAGGAGG GTGAACCGCT GGAGCCTGGG GAGCTCCAGC 
     1921 TGAACGAGCT GACTGTGGAG AGCGTGCAGC ACACGCTGAC CTCAGTGACA GATGAGCTGG 
     1981 CTGTGGCCAC CGAGATGGTG TTCAGGCGGC AGGAGATGGT TACGCAGCTG CAACAGGAGC 
     2041 TCCGGAATGA AGAGGAGAAC ACCCACCCCC GGGAGCGGGT GCAGCTGCTG GGCAAGAGGC 
     2101 AAGTGCTGCA AGAAGCACTG CAGGGGCTGC AGGTAGCGCT GTGCAGCCAG GCCAAGCTGC 
     2161 AGGCCCAGCA GGAGTTGCTG CAGACCAAGC TGGAGCACCT GGGCCCCGGC GAGCCCCCGC 
     2221 CTGTGCTGCT CCTGCAGGAT GACCGCCACT CCACGTCGTC CTCGGAGCAG GAGCGAGAGG 
     2281 GGGGAAGGAC ACCCACGCTG GAGATCCTTA AGAGCCACAT CTCAGGAATC TTCCGCCCCA 
     2341 AGTTCTCGCT CCCTCCACCG CTGCAGCTCA TTCCGGAGGT GCAGAAGCCC CTGCATGAGC 
     2401 AGCTGTGGTA CCACGGGGCC ATCCCGAGGG CAGAGGTGGC TGAGCTGCTG GTGCACTCTG 
     2461 GGGACTTCCT GGTGCGGGAG AGCCAGGGCA AGCAGGAGTA CGTGCTGTCG GTGCTGTGGG 
     2521 ATGGTCTGCC CCGGCACTTC ATCATCCAGT CCTTGGATAA CCTGTACCGA CTGGAAGGGG 
     2581 AAGGCTTTCC TAGCATTCCT TTGCTCATCG ACCACCTACT GAGCACCCAG CAGCCCCTCA 
     2641 CCAAGAAGAG TGGTGTTGTC CTGCACAGGG CTGTGCCCAA GGACAAGTGG GTGCTGAACC 
     2701 ATGAGGACCT GGTGTTGGGT GAGCAGATTG GACGGGGGAA CTTTGGCGAA GTGTTCAGCG 
     2761 GACGCCTGCG AGCCGACAAC ACCCTGGTGG CGGTGAAGTC TTGTCGAGAG ACGCTCCCAC 
     2821 CTGACCTCAA GGCCAAGTTT CTACAGGAAG CGAGGATCCT GAAGCAGTAC AGCCACCCCA 
     2881 ACATCGTGCG TCTCATTGGT GTCTGCACCC AGAAGCAGCC CATCTACATC GTCATGGAGC 
     2941 TTGTGCAGGG GGGCGACTTC CTGACCTTCC TCCGCACGGA GGGGGCCCGC CTGCGGGTGA 
     3001 AGACTCTGCT GCAGATGGTG GGGGATGCAG CTGCTGGCAT GGAGTACCTG GAGAGCAAGT 
     3061 GCTGCATCCA CCGGGACCTG GCTGCTCGGA ACTGCCTGGT GACAGAGAAG AATGTCCTGA 
     3121 AGATCAGTGA CTTTGGGATG TCCCGAGAGG AAGCCGATGG GGTCTATGCA GCCTCAGGGG 
     3181 GCCTCAGACA AGTCCCCGTG AAGTGGACCG CACCTGAGGC CCTTAACTAC GGCCGCTACT 
     3241 CCTCCGAAAG CGACGTGTGG AGCTTTGGCA TCTTGCTCTG GGAGACCTTC AGCCTGGGGG 
     3301 CCTCCCCCTA TCCCAACCTC AGCAATCAGC AGACACGGGA GTTTGTGGAG AAGGGGGGCC 
     3361 GTCTGCCCTG CCCAGAGCTG TGTCCTGATG CCGTGTTCAG GCTCATGGAG CAGTGCTGGG 
     3421 CCTATGAGCC TGGGCAGCGG CCCAGCTTCA GCACCATCTA CCAGGAGCTG CAGAGCATCC 
     3481 GAAAGCGGCA TCGGACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3541 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3601 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3661 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3721 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3781 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     3841 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     3901 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     3961 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4021 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4081 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4141 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4201 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4261 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4321 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4381 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4441 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4501 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4561 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4621 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4681 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4741 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4801 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4861 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     4921 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     4981 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5041 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5101 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5161 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5221 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5281 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5341 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5401 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5461 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5521 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5581 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5641 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5701 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5761 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     5821 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     5881 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     5941 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6001 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6061 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6121 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6181 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6241 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6301 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6361 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6421 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6481 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6541 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6601 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6661 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6721 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6781 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     6841 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     6901 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     6961 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7021 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7081 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7141 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7201 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7261 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7321 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7381 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7441 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7501 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7561 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7621 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7681 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7741 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7801 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     7861 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     7921 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     7981 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8041 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8101 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8161 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8221 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8281 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8341 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8401 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8461 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8521 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8581 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8641 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8701 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8761 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     8821 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     8881 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     8941 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9001 TTTAACAAAA ATTTAACGCG AATTTT
//