LOCUS       RG204120                9224 bp    DNA     circular  SYN 
DEFINITION  MOCOS (tGFP-tagged) - Human molybdenum cofactor sulfurase (MOCOS)
ACCESSION   NM_017947
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3705..4409 
                     /label="GFP"  
     misc_feature    4456..5041 
                     /label="PolyA signal"  
     misc_feature    7133..7778 
                     /label="ColE1"  
     misc_feature    5495..6289 
                     /label="Neo"  
     misc_feature    5126..5428 
                     /label="SV40 ori"  
     misc_feature    7793..8653 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3684..3690 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3684 
                     /label="MOCOS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGCGCG GCGGCGGAGT CAGGGCGGGA GCTGTGGACC TTCGCGGGTT 
     1081 CCCGGGACCC GAGCGCACCG CGGCTAGCCT ACGGCTACGG CCCGGGCAGC CTGCGCGAGC 
     1141 TGCGGGCGCG CGAGTTCAGC CGCCTGGCAG GAACTGTCTA TCTTGACCAT GCAGGTGCCA 
     1201 CCTTGTTCTC CCAGAGCCAG CTCGAAAGCT TCACTAGTGA TCTCATGGAA AACACTTATG 
     1261 GTAATCCTCA CAGCCAGAAC ATCAGCAGCA AGCTCACCCA TGACACTGTG GAGCAGGTGC 
     1321 GCTACAGAAT CCTGGCGCAC TTCCACACCA CCGCAGAAGA CTACACTGTG ATCTTCACTG 
     1381 CCGGGAGCAC GGCTGCTCTC AAACTGGTGG CAGAGGCCTT TCCATGGGTG TCCCAGGGCC 
     1441 CAGAGAGCAG TGGGAGTCGC TTCTGTTACC TCACCGACAG CCACACCTCC GTAGTGGGTA 
     1501 TGAGGAACGT GACCATGGCT ATAAATGTCA TATCCATCCC GGTCAGGCCA GAGGACCTGT 
     1561 GGTCTGCAGA GGAACGTGGT GCTTCAGCCA GCAACCCAGA CTGCCAGCTG CCGCATCTCT 
     1621 TCTGCTACCC AGCTCAGAGT AACTTTTCTG GAGTCAGATA CCCCCTGTCC TGGATAGAAG 
     1681 AGGTCAAGTC TGGGCGGTTG CGCCCTGTGA GCACGCCTGG GAAGTGGTTT GTGCTGCTGG 
     1741 ATGCAGCCTC CTACGTGAGC ACCTCGCCTT TGGACCTGTC AGCTCACCAG GCCGACTTTG 
     1801 TCCCCATCTC CTTCTATAAG ATCTTCGGGT TTCCTACAGG CCTGGGCGCT CTGCTGGTCC 
     1861 ATAATCGTGC GGCTCCTCTA CTGAGGAAGA CCTACTTTGG AGGAGGGACA GCCTCTGCGT 
     1921 ACCTAGCAGG AGAAGACTTC TACATCCCGA GGCAGTCGGT AGCTCAGAGG TTTGAAGATG 
     1981 GCACCATCTC ATTCCTTGAT GTTATCGCGC TAAAACATGG ATTTGACACC CTAGAGCGCC 
     2041 TCACAGGTGG AATGGAGAAT ATAAAGCAGC ACACCTTCAC CTTGGCTCAG TATACCTACA 
     2101 TGGCCCTGTC CTCTCTCCAG TACCCCAATG GAGCCCCTGT GGTGCGGATT TACAGCGATT 
     2161 CTGAGTTCAG CAGCCCTGAG GTTCAGGGCC CGATCATCAA TTTTAATGTG CTGGATGACA 
     2221 AAGGGAACAT CATTGGTTAC TCCCAGGTGG ACAAAATGGC CAGTCTTTAC AACATCCACC 
     2281 TGCGAACTGG CTGCTTCTGT AACACTGGGG CCTGCCAGAG GCACCTGGGC ATAAGCAACG 
     2341 AGATGGTCAG GAAGCATTTT CAGGCTGGTC ATGTCTGTGG GGACAATATG GACCTCATAG 
     2401 ATGGGCAGCC CACAGGATCT GTGAGGATTT CATTTGGATA CATGTCGACG CTGGATGATG 
     2461 TCCAGGCCTT TCTTAGGTTC ATCATAGACA CTCGCCTGCA CTCATCAGGG GACTGGCCTG 
     2521 TCCCTCAGGC CCATGCTGAC ACCGGGGAGA CTGGAGCCCC ATCAGCAGAC AGCCAGGCTG 
     2581 ATGTTATACC TGCTGTCATG GGCAGACGTA GCCTCTCGCC TCAGGAAGAT GCCCTCACAG 
     2641 GCTCCAGGGT TTGGAACAAC TCGTCTACTG TGAATGCTGT GCCTGTGGCC CCACCTGTGT 
     2701 GTGATGTCGC CAGAACCCAG CCGACTCCTT CAGAGAAAGC TGCAGGAGTC CTGGAGGGGG 
     2761 CCCTTGGGCC ACATGTTGTC ACTAACCTTT ATCTCTATCC AATCAAATCC TGTGCTGCAT 
     2821 TTGAGGTGAC CAGGTGGCCT GTAGGAAACC AAGGGCTGCT ATATGACCGG AGCTGGATGG 
     2881 TTGTGAATCA CAATGGTGTT TGCCTGAGTC AGAAGCAGGA ACCCCGGCTC TGCCTGATCC 
     2941 AGCCCTTCAT CGACTTGCGG CAAAGGATCA TGGTCATCAA AGCCAAAGGG ATGGAGCCTA 
     3001 TAGAGGTGCC TCTTGAGGAA AATAGTGAAC GGACTCAGAT TCGCCAAAGC AGGGTCTGTG 
     3061 CTGACAGAGT AAGTACTTAT GATTGTGGAG AAAAAATTTC AAGCTGGTTG TCAACATTTT 
     3121 TTGGCCGTCC TTGTAATTTG ATCAAACAAA GTTCAAACTC TCAAAGGAAT GCAAAGAAGA 
     3181 AACATGGAAA AGATCAACTT CCTGGTACAA TGGCCACCCT TTCTCTGGTG AATGAGGCAC 
     3241 AGTATCTGCT GATCAACACA TCCAGTATTT TGGAACTTCA CCGGCAACTA AACACCAGTG 
     3301 ATGAGAATGG AAAGGAGGAA TTATTCTCAC TGAAGGATCT CAGCTTGCGT TTTCGTGCCA 
     3361 ATATTATTAT CAATGGAAAA AGGGCTTTTG AAGAAGAGAA ATGGGATGAG ATTTCAATTG 
     3421 GCTCTTTGCG TTTCCAGGTT TTGGGGCCTT GTCACAGATG CCAGATGATT TGCATCGACC 
     3481 AGCAAACTGG GCAACGAAAC CAGCATGTTT TCCAAAAACT TTCTGAGAGT CGTGAAACAA 
     3541 AGGTGAACTT TGGCATGTAC CTGATGCATG CATCATTGGA TTTATCCTCC CCATGTTTCC 
     3601 TGTCTGTAGG ATCTCAGGTG CTCCCTGTGT TGAAAGAGAA TGTGGAAGGT CATGATTTAC 
     3661 CTGCATCTGA GAAACACCAG GATGTTACCT CCACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3721 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3781 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3841 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3901 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3961 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4021 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4081 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4141 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4201 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4261 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4321 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4381 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4441 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4501 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4561 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4621 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4681 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4741 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4801 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4861 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4921 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4981 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5041 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5101 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5161 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5221 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5281 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5341 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5401 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5461 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5521 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5581 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5641 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5701 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5761 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5821 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5881 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5941 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6001 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6061 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6121 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6181 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6241 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6301 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6361 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6421 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6481 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6541 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6601 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6661 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6721 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6781 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6841 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6901 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6961 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7021 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7081 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7141 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7201 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7261 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7321 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7381 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7441 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7501 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7561 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7621 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7681 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7741 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7801 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7861 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7921 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7981 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8041 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8101 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8161 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8221 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8281 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8341 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8401 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8461 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8521 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8581 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8641 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8701 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8761 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8821 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8881 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8941 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9001 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9061 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9121 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9181 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//