LOCUS       RG203918                9002 bp    DNA     circular  SYN 
DEFINITION  AFAP1L2 (tGFP-tagged) - Human actin filament associated protein 1-like 2 (AFAP1L2), transcript variant 2
ACCESSION   NM_032550
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3483..4187 
                     /label="GFP"  
     misc_feature    4234..4819 
                     /label="PolyA signal"  
     misc_feature    6911..7556 
                     /label="ColE1"  
     misc_feature    5273..6067 
                     /label="Neo"  
     misc_feature    4904..5206 
                     /label="SV40 ori"  
     misc_feature    7571..8431 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3462..3468 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3462 
                     /label="AFAP1L2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGGTAC AAAGCCCTGG AACAGCTGCT GACAGAGTTG GATGACTTCC 
     1081 TCAAGATTCT TGACCAGGAG AACCTGAGCA GCACAGCACT GGTGAAGAAG AGCTGCCTGG 
     1141 CGGAGCTCCT CCGGCTTTAC ACCAAAAGCA GCAGCTCTGA TGAGGAGTAC ATTTATATGA 
     1201 ACAAAGTGAC CATCAACAAG CAACAGAATG CAGAGTCTCA AGGCAAAGCG CCTGAGGAGC 
     1261 AGGGCCTGCT ACCCAATGGG GAGCCCAGCC AGCACTCCTC GGCCCCTCAG AAGAGCCTTC 
     1321 CAGACCTCCC GCCACCCAAG ATGATTCCAG AACGGAAACA GCTTGCCATC CCAAAGACGG 
     1381 AGTCTCCAGA GGGCTACTAT GAAGAGGCTG AGCCATATGA CACATCCCTC AATGAGGACG 
     1441 GAGAGGCTGT GAGCAGCTCC TACGAGTCCT ACGATGAAGA GGACGGCAGC AAGGGCAAGT 
     1501 CGGCCCCTTA CCAGTGGCCC TCGCCGGAGG CCGGCATCGA GCTGATGCGT GACGCCCGCA 
     1561 TCTGCGCCTT CCTGTGGCGC AAGAAGTGGC TGGGACAGTG GGCCAAGCAG CTCTGTGTCA 
     1621 TCAAGGACAA CAGGCTTCTG TGCTACAAAT CCTCCAAGGA CCACAGCCCT CAGCTGGACG 
     1681 TGAACCTACT GGGCAGCAGC GTCATTCACA AGGAGAAGCA AGTGCGGAAG AAGGAGCACA 
     1741 AGCTGAAGAT CACACCGATG AATGCCGATG TGATTGTGCT GGGCCTGCAG AGCAAGGACC 
     1801 AGGCTGAGCA GTGGCTCAGG GTCATCCAGG AAGTGAGCGG CCTGCCTTCC GAAGGAGCAT 
     1861 CTGAAGGAAA CCAGTACACC CCGGATGCCC AGCGCTTTAA CTGCCAGAAA CCAGATATAG 
     1921 CTGAGAAGTA CCTGTCGGCT TCAGAGTATG GGAGCTCCGT GGATGGCCAC CCTGAGGTCC 
     1981 CAGAAACCAA AGACGTCAAG AAGAAATGTT CTGCTGGCCT CAAACTGAGC AACCTAATGA 
     2041 ATCTGGGCAG GAAGAAATCC ACCTCACTGG AGCCTGTGGA GAGGTCCCTC GAGACATCCA 
     2101 GTTACCTGAA CGTGCTGGTG AACAGCCAGT GGAAGTCTCG CTGGTGCTCT GTCAGGGACA 
     2161 ATCACCTGCA CTTCTACCAG GACCGGAACC GGAGCAAGGT GGCCCAGCAA CCCCTCAGCC 
     2221 TGGTGGGCTG CGAGGTGGTC CCAGACCCCA GCCCCGACCA CCTCTACTCC TTCCGCATCC 
     2281 TCCACAAGGG CGAGGAGCTG GCCAAGCTTG AGGCCAAGTC TTCCGAGGAA ATGGGCCACT 
     2341 GGCTGGGTCT CCTGCTCTCT GAGTCAGGCT CCAAGACAGA CCCAGAAGAG TTCACCTACG 
     2401 ACTATGTGGA TGCCGATAGG GTCTCCTGTA TTGTGAGTGC GGCCAAAAAC TCTCTCTTAC 
     2461 TGATGCAGAG AAAGTTCTCA GAGCCCAACA CTTACATCGA TGGCCTGCCT AGCCAGGACC 
     2521 GCCAGGAGGA GCTGTATGAC GACGTGGACC TGTCAGAGCT CACAGCTGCG GTGGAGCCTA 
     2581 CCGAGGAAGC CACCCCTGTT GCAGATGACC CAAATGAGAG AGAATCTGAC CGGGTGTACC 
     2641 TGGACCTCAC ACCTGTCAAG TCCTTTCTGC ATGGCCCCAG CAGTGCACAG GCCCAGGCCT 
     2701 CCTCCCCGAC GTTGTCCTGC CTGGACAATG CAACTGAGGC CCTCCCGGCA GACTCAGGCC 
     2761 CAGGTCCCAC CCCAGATGAG CCCTGCATAA AGTGTCCAGA GAACCTGGGA GAACAGCAGC 
     2821 TGGAGAGTTT GGAGCCAGAG GATCCTTCCC TGAGAATCAC CACCGTCAAA ATCCAGACGG 
     2881 AACAGCAGAG AATCTCCTTC CCACCGAGCT GCCCGGATGC CGTGGTGGCC ACCCCACCTG 
     2941 GTGCCAGCCC ACCTGTGAAG GACAGGTTGC GCGTGACCAG TGCAGAGATC AAGCTTGGCA 
     3001 AGAATCGGAC AGAAGCTGAG GTGAAGCGGT ACACAGAGGA GAAGGAGAGG CTTGAAAAGA 
     3061 AGAAGGAAGA AATCCGGGGG CACCTGGCTC AGCTCCGGAA AGAGAAACGG GAGCTAAAGG 
     3121 AAACCCTACT GAAATGCACA GACAAGGAAG TCCTGGCGAG CCTGGAGCAG AAGCTGAAGG 
     3181 AAATTGACGA GGAGTGCCGG GGCGAGGAGA GCAGGCGCGT GGACCTGGAG CTCAGCATCA 
     3241 TGGAGGTGAA GGACAACCTG AAGAAGGCTG AGGCAGGGCC TGTGACGTTA GGCACCACCG 
     3301 TGGACACCAC CCACCTGGAG AATCCCAAAG CTGTCACACC TGCCTCTGCC CCAGACTGTA 
     3361 CCCCAGTCAA CTCTGCAACC ACACTCAAGA ACAGGCCTCT CTCGGTCGTG GTCACAGGCA 
     3421 AAGGCACTGT ACTCCAGAAA GCCAAGGAAT GGGAGAAGAA AGGAGCAAGT ACGCGTACGC 
     3481 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3541 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3601 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3661 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3721 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     3781 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     3841 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     3901 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     3961 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4021 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4081 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4141 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4201 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4261 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4321 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4381 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4441 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4501 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4561 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4621 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4681 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4741 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4801 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4861 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     4921 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     4981 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5041 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5101 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5161 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5221 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5281 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5341 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5401 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5461 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5521 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5581 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5641 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5701 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     5761 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     5821 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     5881 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     5941 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6001 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6061 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6121 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6181 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6241 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6301 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6361 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6421 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6481 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6541 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6601 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6661 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6721 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     6781 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     6841 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     6901 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     6961 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7021 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7081 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7141 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7201 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7261 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7321 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7381 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7441 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7501 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7561 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7621 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7681 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7741 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     7801 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     7861 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     7921 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     7981 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8041 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8101 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8161 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8221 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8281 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8341 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8401 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8461 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8521 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8581 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8641 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8701 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     8761 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     8821 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     8881 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     8941 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9001 TT
//