LOCUS       RG203858                9197 bp    DNA     circular  SYN 
DEFINITION  MASTL (tGFP-tagged) - Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2
ACCESSION   NM_032844
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3678..4382 
                     /label="GFP"  
     misc_feature    4429..5014 
                     /label="PolyA signal"  
     misc_feature    7106..7751 
                     /label="ColE1"  
     misc_feature    5468..6262 
                     /label="Neo"  
     misc_feature    5099..5401 
                     /label="SV40 ori"  
     misc_feature    7766..8626 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3657..3663 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3657 
                     /label="MASTL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCCACC GCGGGAAGCA AGAAGGAGCC TGGAGGAGGC GCGGCGACTG 
     1081 AGGAGGGCGT GAATAGGATC GCAGTGCCAA AACCGCCCTC CATTGAGGAA TTCAGCATAG 
     1141 TGAAGCCCAT TAGCCGGGGC GCCTTCGGGA AAGTGTATCT GGGGCAGAAA GGCGGCAAAT 
     1201 TGTATGCAGT AAAGGTTGTT AAAAAAGCAG ACATGATCAA CAAAAATATG ACTCATCAGG 
     1261 TCCAAGCTGA GAGAGATGCA CTGGCACTAA GCAAAAGCCC ATTCATTGTC CATTTGTATT 
     1321 ATTCACTGCA GTCTGCAAAC AATGTCTACT TGGTAATGGA ATATCTTATT GGGGGAGATG 
     1381 TCAAGTCTCT CCTACATATA TATGGTTATT TTGATGAAGA GATGGCTGTG AAATATATTT 
     1441 CTGAAGTAGC ACTGGCTCTA GACTACCTTC ACAGACATGG AATCATCCAC AGGGACTTGA 
     1501 AACCGGACAA TATGCTTATT TCTAATGAGG GTCATATTAA ACTGACGGAT TTTGGCCTTT 
     1561 CAAAAGTTAC TTTGAATAGA GATATTAATA TGATGGATAT CCTTACAACA CCATCAATGG 
     1621 CAAAACCTAG ACAAGATTAT TCAAGAACCC CAGGACAAGT GTTATCGCTT ATCAGCTCGT 
     1681 TGGGATTTAA CACACCAATT GCAGAAAAAA ATCAAGACCC TGCAAACATC CTTTCAGCCT 
     1741 GTCTGTCTGA AACATCACAG CTTTCTCAAG GACTCGTATG CCCTATGTCT GTAGATCAAA 
     1801 AGGACACTAC GCCTTATTCT AGCAAATTAC TAAAATCATG TCTTGAAACA GTTGCCTCCA 
     1861 ACCCAGGAAT GCCTGTGAAG TGTCTAACTT CTAATTTACT CCAGTCTAGG AAAAGGCTGG 
     1921 CCACATCCAG TGCCAGTAGT CAATCCCACA CCTTCATATC CAGTGTGGAA TCAGAATGCC 
     1981 ACAGCAGTCC CAAATGGGAA AAAGATTGCC AGGAAAGTGA TGAAGCATTG GGCCCAACAA 
     2041 TGATGAGTTG GAATGCAGTT GAAAAGTTAT GCGCAAAATC TGCAAATGCC ATTGAGACGA 
     2101 AAGGTTTCAA TAAAAAGGAT CTGGAGTTAG CTCTTTCTCC CATTCATAAC AGCAGTGCCC 
     2161 TTCCCACCAC TGGACGCTCT TGTGTAAACC TTGCTAAAAA ATGCTTCTCT GGGGAAGTTT 
     2221 CTTGGGAAGC AGTAGAACTG GATGTAAATA ATATAAATAT GGACACTGAC ACAAGTCAGT 
     2281 TAGGTTTCCA TCAGTCAAAT CAGTGGGCTG TGGATTCTGG TGGGATATCT GAAGAGCACC 
     2341 TTGGGAAAAG AAGTTTAAAA AGAAATTTTG AGTTGGTTGA CTCCAGTCCT TGTAAAAAAA 
     2401 TTATACAGAA TAAAAAAACT TGTGTAGAGT ATAAGCATAA CGAAATGACA AATTGTTATA 
     2461 CAAATCAAAA TACAGGCTTA ACAGTTGAAG TGCAGGACCT TAAGCTATCA GTGCACAAAA 
     2521 GTCAACAAAA TGACTGTGCT AATAAGGAGA ACATTGTCAA TTCTTTTACT GATAAACAAC 
     2581 AAACACCAGA AAAATTACCT ATACCAATGA TAGCAAAAAA CCTTATGTGT GAACTCGATG 
     2641 AAGACTGTGA AAAGAATAGT AAGAGGGACT ACTTAAGTTC TAGTTTTCTA TGTTCTGATG 
     2701 ATGATAGAGC TTCTAAAAAT ATTTCTATGA ACTCTGATTC ATCTTTTCCT GGAATTTCTA 
     2761 TAATGGAAAG TCCATTAGAA AGTCAGCCCT TAGATTCAGA TAGAAGCATT AAAGAATCCT 
     2821 CTTTTGAAGA ATCAAATATT GAAGATCCAC TTATTGTAAC ACCAGATTGC CAAGAAAAGA 
     2881 CCTCACCAAA AGGTGTCGAG AACCCTGCTG TACAAGAGAG TAACCAAAAA ATGTTAGGTC 
     2941 CTCCTTTGGA GGTGCTGAAA ACGTTAGCCT CTAAAAGAAA TGCTGTTGCT TTTCGAAGTT 
     3001 TTAACAGTCA TATTAATGCA TCCAATAACT CAGAACCATC CAGAATGAAC ATGACTTCTT 
     3061 TAGATGCAAT GGATATTTCG TGTGCCTACA GTGGTTCATA TCCCATGGCT ATAACCCCTA 
     3121 CTCAAAAAAG AAGATCCTGT ATGCCACATC AGCAGACCCC AAATCAGATC AAGTCGGGAA 
     3181 CTCCATACCG AACTCCGAAG AGTGTGAGAA GAGGGGTGGC CCCCGTTGAT GATGGGCGAA 
     3241 TTCTAGGAAC CCCAGACTAC CTTGCACCTG AGCTGTTACT AGGCAGGGCC CATGGTCCTG 
     3301 CGGTAGACTG GTGGGCACTT GGAGTTTGCT TGTTTGAATT TCTAACAGGA ATTCCCCCTT 
     3361 TCAATGATGA AACACCACAA CAAGTATTCC AGAATATTCT GAAAAGAGAT ATCCCTTGGC 
     3421 CAGAAGGTGA AGAAAAGTTA TCTGATAATG CTCAAAGTGC AGTAGAAATA CTTTTAACCA 
     3481 TTGATGATAC AAAGAGAGCT GGAATGAAAG AGCTAAAACG TCATCCTCTC TTCAGTGATG 
     3541 TGGACTGGGA AAATCTGCAG CATCAGACTA TGCCTTTCAT CCCCCAGCCA GATGATGAAA 
     3601 CAGATACCTC CTATTTTGAA GCCAGGAATA CTGCTCAGCA CCTGACCGTA TCTGGATTTA 
     3661 GTCTGACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3721 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3781 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3841 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     3901 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     3961 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4021 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4081 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4141 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4201 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4261 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4321 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4381 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4441 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4501 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4561 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4621 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4681 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4741 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4801 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4861 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4921 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4981 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5041 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5101 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5161 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5221 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5281 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5341 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5401 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5461 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5521 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5581 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5641 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5701 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5761 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5821 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     5881 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     5941 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6001 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6061 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6121 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6181 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6241 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6301 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6361 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6421 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6481 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6541 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6601 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6661 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6721 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6781 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6841 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     6901 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     6961 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7021 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7081 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7141 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7201 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7261 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7321 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7381 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7441 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7501 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7561 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7621 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7681 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7741 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7801 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7861 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     7921 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     7981 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8041 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8101 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8161 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8221 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8281 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8341 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8401 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8461 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8521 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8581 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8641 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8701 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8761 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8821 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     8881 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     8941 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9001 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9061 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9121 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9181 AATTTAACGC GAATTTT
//