LOCUS       RG202624                9065 bp    DNA     circular  SYN 
DEFINITION  USP5 (tGFP-tagged) - Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2
ACCESSION   NM_003481
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3546..4250 
                     /label="GFP"  
     misc_feature    4297..4882 
                     /label="PolyA signal"  
     misc_feature    6974..7619 
                     /label="ColE1"  
     misc_feature    5336..6130 
                     /label="Neo"  
     misc_feature    4967..5269 
                     /label="SV40 ori"  
     misc_feature    7634..8494 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3525..3531 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3525 
                     /label="USP5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGCTG AGTGAGGAGG CGCTGCTGTC AGTATTACCG ACGATCCGGG 
     1081 TCCCTAAGGC TGGAGACCGG GTCCACAAAG ACGAGTGCGC CTTCTCCTTC GACACGCCGG 
     1141 AGTCTGAGGG GGGCCTCTAC ATCTGTATGA ACACGTTTCT GGGCTTTGGG AAACAGTATG 
     1201 TGGAGAGACA TTTCAATAAG ACCGGCCAGC GAGTCTACTT GCACCTCCGG CGGACCCGGC 
     1261 GCCCGAAAGA GGAGGACCCT GCTACAGGCA CTGGAGACCC ACCCCGGAAG AAGCCCACGC 
     1321 GGCTGGCTAT TGGTGTTGAA GGCGGATTTG ACCTTAGCGA GGAGAAGTTT GAATTAGACG 
     1381 AGGATGTGAA GATTGTCATT TTGCCAGATT ACCTGGAGAT TGCCCGGGAT GGACTGGGGG 
     1441 GACTGCCTGA CATTGTCAGA GATCGGGTGA CCAGTGCAGT GGAGGCCCTA CTGTCGGCCG 
     1501 ACTCAGCCTC CCGCAAGCAG GAGGTGCAGG CATGGGATGG GGAAGTACGG CAGGTGTCTA 
     1561 AGCATGCCTT CAGCCTCAAG CAGTTGGACA ACCCTGCTCG AATCCCTCCC TGTGGCTGGA 
     1621 AGTGCTCCAA GTGTGACATG AGAGAGAACC TGTGGCTCAA CCTGACTGAT GGCTCCATCC 
     1681 TCTGTGGGCG ACGCTACTTC GATGGCAGTG GGGGCAACAA CCACGCTGTG GAGCACTACC 
     1741 GAGAGACAGG CTACCCGTTA GCTGTCAAGC TGGGCACCAT CACCCCTGAT GGAGCTGACG 
     1801 TGTACTCATA TGATGAGGAT GACATGGTCC TGGACCCCAG CCTGGCTGAG CACCTGTCCC 
     1861 ACTTCGGCAT CGACATGCTG AAGATGCAGA AGACAGACAA GACGATGACT GAGTTGGAGA 
     1921 TAGACATGAA CCAGCGGATT GGTGAATGGG AGCTGATCCA GGAGTCAGGT GTGCCACTCA 
     1981 AGCCCCTGTT TGGGCCTGGC TACACAGGCA TCCGGAACCT GGGTAACAGC TGCTACCTCA 
     2041 ACTCTGTGGT CCAGGTGCTC TTCAGCATCC CTGACTTCCA GAGGAAGTAT GTGGATAAGC 
     2101 TGGAGAAGAT CTTCCAGAAT GCCCCGACGG ACCCTACCCA GGATTTCAGC ACCCAGGTGG 
     2161 CCAAGCTGGG CCATGGCCTT CTCTCCGGGG AGTATTCCAA GCCAGTACCG GAGTCGGGCG 
     2221 ATGGGGAGCG GGTGCCAGAA CAGAAGGAAG TTCAAGATGG CATTGCCCCT CGGATGTTCA 
     2281 AGGCCCTCAT CGGCAAGGGC CACCCTGAAT TCTCCACCAA CCGGCAGCAG GATGCCCAGG 
     2341 AGTTCTTCCT TCACCTTATC AACATGGTGG AGAGGAATTG CCGGAGCTCT GAAAATCCTA 
     2401 ATGAAGTGTT CCGCTTCTTG GTGGAGGAAA AGATCAAGTG CCTGGCCACA GAGAAGGTGA 
     2461 AGTACACCCA GCGAGTTGAC TACATCATGC AGCTGCCTGT GCCCATGGAT GCAGCCCTTA 
     2521 ACAAAGAGGA GCTTCTGGAG TACGAGGAGA AGAAGCGGCA AGCCGAAGAG GAGAAGATGG 
     2581 CACTGCCAGA ACTGGTTCGG GCCCAGGTGC CCTTCAGCTC TTGCCTGGAG GCCTACGGGG 
     2641 CCCCTGAGCA GGTCGATGAC TTCTGGAGCA CGGCCCTGCA GGCCAAGTCA GTAGCTGTCA 
     2701 AGACCACACG ATTTGCCTCA TTCCCTGACT ACCTGGTCAT CCAGATCAAG AAGTTCACCT 
     2761 TCGGCTTAGA CTGGGTGCCC AAGAAACTGG ATGTGTCCAT CGAGATGCCA GAGGAGCTCG 
     2821 ACATCTCCCA GTTGAGGGGC ACAGGGCTGC AGCCCGGAGA GGAGGAGCTG CCAGACATTG 
     2881 CCCCACCCCT GGTCACTCCG GATGAGCCCA AAGCGCCCAT GCTGGATGAA TCAGTCATCA 
     2941 TCCAGCTGGT GGAGATGGGA TTCCCTATGG ACGCCTGCCG CAAAGCTGTC TACTACACGG 
     3001 GCAACAGCGG GGCTGAGGCC GCCATGAACT GGGTCATGTC ACACATGGAT GATCCAGATT 
     3061 TTGCAAACCC CCTCATCCTG CCTGGCTCTA GTGGGCCGGG CTCCACAAGC GCAGCAGCCG 
     3121 ACCCCCCTCC TGAGGACTGT GTGACCACCA TTGTCTCCAT GGGCTTCTCC CGGGACCAGG 
     3181 CCTTGAAAGC GCTGCGGGCC ACGAACAATA GTTTAGAACG GGCTGTGGAC TGGATCTTCA 
     3241 GTCACATTGA CGACCTGGAT GCTGAAGCTG CCATGGACAT CTCAGAGGGC CGCTCAGCTG 
     3301 CCGACTCCAT CTCTGAGTCT GTGCCAGTGG GACCTAAAGT CCGGGATGGT CCTGGAAAGT 
     3361 ATCAGCTCTT TGCCTTCATT AGTCACATGG GCACCTCTAC CATGTGTGGT CACTACGTCT 
     3421 GCCACATCAA GAAAGAAGGC AGATGGGTGA TCTACAATGA CCAGAAAGTG TGTGCCTCCG 
     3481 AGAAGCCGCC CAAGGACCTG GGCTACATCT ACTTCTACCA GAGAGTGGCC AGCACGCGTA 
     3541 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3601 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3661 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3721 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     3781 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     3841 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     3901 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     3961 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4021 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4081 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4141 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4201 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4261 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4321 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4381 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4441 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4501 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4561 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4621 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4681 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4741 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4801 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4861 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4921 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     4981 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5041 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5101 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5161 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5221 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5281 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5341 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5401 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5461 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5521 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5581 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5641 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5701 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     5761 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     5821 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     5881 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     5941 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6001 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6061 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6121 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6181 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6241 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6301 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6361 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6421 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6481 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6541 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6601 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6661 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6721 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     6781 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     6841 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     6901 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     6961 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7021 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7081 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7141 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7201 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7261 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7321 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7381 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7441 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7501 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7561 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7621 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7681 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7741 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     7801 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     7861 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     7921 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     7981 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8041 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8101 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8161 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8221 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8281 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8341 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8401 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8461 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8521 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8581 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8641 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8701 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     8761 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     8821 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     8881 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     8941 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9001 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9061 ATTTT
//