LOCUS       RG202190                9149 bp    DNA     circular  SYN 
DEFINITION  USP13 (tGFP-tagged) - Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13)
ACCESSION   NM_003940
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3630..4334 
                     /label="GFP"  
     misc_feature    4381..4966 
                     /label="PolyA signal"  
     misc_feature    7058..7703 
                     /label="ColE1"  
     misc_feature    5420..6214 
                     /label="Neo"  
     misc_feature    5051..5353 
                     /label="SV40 ori"  
     misc_feature    7718..8578 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3609..3615 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3609 
                     /label="USP13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCGCCGG GGCGCCCTGT TCGGCATGCC GGGCGGCAGC GGAGGCAGGA 
     1081 AGATGGCTGC AGGAGACATC GGCGAGCTGC TAGTGCCCCA CATGCCCACG ATCCGCGTGC 
     1141 CCAGGTCCGG CGACAGGGTC TACAAGAACG AGTGCGCCTT CTCCTACGAC TCTCCCAATT 
     1201 CTGAAGGTGG ACTCTATGTA TGCATGAATA CATTTTTGGC CTTTGGAAGG GAACATGTTG 
     1261 AAAGACATTT TCGAAAAACT GGACAGAGTG TATACATGCA CCTGAAAAGA CATGTGCGAG 
     1321 AGAAGGTAAG AGGGGCGTCT GGTGGAGCGT TACCAAAAAG GAGGAATTCC AAGATTTTTT 
     1381 TAGATCTAGA TACTGATGAC GATTTAAATA GCGACGATTA TGAATATGAA GATGAAGCCA 
     1441 AACTTGTTAT ATTCCCAGAT CACTATGAAA TAGCACTACC AAATATTGAG GAGTTACCAG 
     1501 CCCTGGTAAC AATTGCTTGT GATGCAGTTC TCAGCTCAAA ATCTCCATAC AGAAAGCAGG 
     1561 ACCCAGACAC GTGGGAAAAT GAATTGCCAG TATCTAAATA TGCCAACAAC CTCACCCAGC 
     1621 TGGACAATGG AGTCAGGATT CCTCCAAGTG GTTGGAAGTG TGCCAGATGC GACCTGCGAG 
     1681 AAAACCTCTG GTTGAATCTG ACTGACGGCT CTGTCCTGTG TGGAAAGTGG TTCTTTGACA 
     1741 GCTCTGGGGG CAACGGGCAT GCGCTGGAGC ATTACAGAGA CATGGGCTAC CCACTAGCCG 
     1801 TGAAACTGGG AACCATCACT CCTGACGGGG CAGATGTTTA TTCTTTTCAA GAAGAAGAAC 
     1861 CTGTTTTGGA TCCTCATTTG GCCAAGCACT TAGCGCATTT TGGAATTGAT ATGCTTCATA 
     1921 TGCATGGGAC AGAGAATGGG CTCCAGGACA ATGACATCAA GCTGAGGGTC AGTGAGTGGG 
     1981 AAGTGATCCA GGAGTCGGGC ACGAAACTGA AGCCAATGTA TGGTCCTGGC TACACGGGTC 
     2041 TGAAGAACCT GGGCAACAGC TGCTATCTCA GCTCTGTCAT GCAGGCCATC TTCAGCATCC 
     2101 CAGAATTCCA GAGAGCGTAT GTAGGAAACC TTCCCAGAAT ATTTGACTAC TCGCCTTTAG 
     2161 ATCCAACACA AGATTTCAAC ACACAGATGA CTAAGTTAGG ACATGGCCTT CTCTCAGGCC 
     2221 AGTATTCAAA GCCTCCGGTG AAATCTGAAC TCATTGAACA GGTGATGAAG GAGGAGCACA 
     2281 AGCCACAGCA GAACGGGATC TCTCCGCGCA TGTTTAAGGC CTTTGTAAGC AAGAGCCACC 
     2341 CGGAATTCTC CTCTAACAGG CAGCAAGATG CCCAGGAATT CTTCTTGCAC CTGGTGAATC 
     2401 TAGTAGAGAG GAACCGCATC GGCTCAGAAA ACCCAAGCGA TGTTTTTCGT TTTTTGGTGG 
     2461 AAGAACGCAT TCAGTGCTGT CAGACCCGGA AAGTCCGCTA CACGGAGAGG GTGGATTACC 
     2521 TGATGCAGTT ACCTGTGGCC ATGGAGGCGG CAACCAACAA GGATGAACTG ATCGCTTATG 
     2581 AACTAACGAG AAGGGAAGCA GAAGCAAACA GAAGACCCCT TCCTGAGTTG GTACGTGCCA 
     2641 AGATACCATT TAGTGCCTGC CTTCAGGCCT TCTCTGAACC AGAAAATGTT GATGATTTCT 
     2701 GGAGCAGTGC CCTACAAGCA AAGTCTGCGG GTGTGAAAAC ATCTCGCTTT GCTTCATTCC 
     2761 CTGAATACTT GGTAGTGCAG ATAAAGAAGT TCACTTTTGG TCTTGACTGG GTTCCCAAAA 
     2821 AATTTGATGT TTCTATTGAT ATGCCAGACC TACTTGATAT CAACCATCTC CGAGCCAGGG 
     2881 GGTTACAGCC AGGAGAGGAA GAACTTCCAG ACATCAGCCC CCCCATAGTC ATTCCTGATG 
     2941 ACTCAAAAGA TCGCCTGATG AACCAATTGA TAGACCCATC AGACATCGAT GAGTCATCAG 
     3001 TGATGCAGCT GGCCGAGATG GGTTTCCCGC TGGAAGCATG TCGCAAGGCT GTGTACTTCA 
     3061 CTGGAAATAT GGGCGCCGAG GTGGCCTTCA ACTGGATCAT TGTTCACATG GAAGAGCCAG 
     3121 ATTTTGCTGA GCCGCTGACC ATGCCTGGTT ATGGAGGGGC AGCTTCTGCT GGAGCCTCTG 
     3181 TTTTTGGTGC TTCTGGACTG GATAACCAAC CTCCAGAGGA AATCGTAGCT ATCATCACCT 
     3241 CCATGGGATT TCAGCGAAAT CAGGCTATTC AGGCACTACG AGCAACGAAT AATAACCTGG 
     3301 AAAGAGCACT GGATTGGATC TTTAGCCACC CTGAGTTTGA AGAAGACAGT GATTTTGTGA 
     3361 TTGAGATGGA GAATAATGCC AATGCAAACA TTATTTCTGA GGCCAAGCCC GAAGGACCTA 
     3421 GAGTCAAGGA TGGATCTGGA ACATATGAGC TATTTGCATT CATCAGTCAC ATGGGAACAT 
     3481 CCACAATGAG TGGTCATTAC ATTTGCCATA TCAAAAAGGA AGGAAGATGG GTGATTTACA 
     3541 ATGACCACAA AGTTTGTGCC TCAGAAAGGC CCCCTAAAGA CCTGGGCTAC ATGTACTTTT 
     3601 ACCGCAGGAT ACCAAGCACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3661 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3721 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3781 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3841 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3901 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     3961 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4021 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4081 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4141 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4201 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4261 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4321 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4381 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4441 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4501 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4561 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4621 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4681 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4741 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4801 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4861 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4921 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4981 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5041 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5101 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5161 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5221 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5281 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5341 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5401 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5461 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5521 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5581 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5641 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5701 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5761 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5821 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5881 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     5941 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6001 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6061 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6121 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6181 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6241 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6301 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6361 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6421 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6481 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6541 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6601 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6661 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6721 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6781 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6841 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6901 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6961 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7021 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7081 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7141 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7201 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7261 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7321 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7381 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7441 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7501 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7561 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7621 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7681 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7741 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7801 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7861 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7921 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     7981 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8041 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8101 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8161 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8221 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8281 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8341 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8401 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8461 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8521 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8581 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8641 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8701 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8761 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8821 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8881 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     8941 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9001 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9061 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9121 TGATTTAACA AAAATTTAAC GCGAATTTT
//