LOCUS       RG202077                9098 bp    DNA     circular  SYN 
DEFINITION  PYGB (tGFP-tagged) - Human phosphorylase, glycogen; brain (PYGB)
ACCESSION   NM_002862
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3577..4281 
                     /label="GFP"  
     misc_feature    4328..4913 
                     /label="PolyA signal"  
     misc_feature    7005..7650 
                     /label="ColE1"  
     misc_feature    5367..6161 
                     /label="Neo"  
     misc_feature    4998..5300 
                     /label="SV40 ori"  
     misc_feature    7665..8525 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3549..3556 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3549 
                     /label="PYGB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAAGCCG CTGACGGACA GCGAGAAGCG GAAGCAGATC AGCGTGCGCG 
     1081 GCCTGGCGGG GCTAGGCGAC GTGGCCGAGG TGCGGAAGAG CTTCAACCGG CACTTGCACT 
     1141 TCACGCTGGT CAAGGACCGC AATGTGGCCA CGCCCCGCGA CTACTTCTTC GCGCTGGCGC 
     1201 ACACGGTGCG CGACCACCTC GTGGGCCGCT GGATCCGCAC GCAGCAGCAC TACTACGAGC 
     1261 GCGACCCCAA GCGCATTTAT TATCTTTCCC TGGAATTCTA CATGGGTCGC ACGCTGCAGA 
     1321 ACACGATGGT GAACCTGGGC CTTCAGAATG CCTGCGATGA AGCCATCTAT CAGTTGGGGT 
     1381 TAGACTTGGA GGAACTCGAG GAGATAGAAG AAGATGCTGG CCTTGGGAAT GGAGGCCTGG 
     1441 GGAGGCTGGC AGCGTGTTTC CTTGACTCAA TGGCTACCTT GGGCCTGGCA GCATACGGCT 
     1501 ATGGAATCCG CTATGAATTT GGGATTTTTA ACCAGAAGAT TGTCAATGGC TGGCAGGTAG 
     1561 AGGAGGCCGA TGACTGGCTG CGCTACGGCA ACCCCTGGGA GAAAGCGCGG CCTGAGTATA 
     1621 TGCTTCCCGT GCACTTCTAC GGACGCGTGG AGCACACCCC CGACGGCGTG AAGTGGCTGG 
     1681 ACACACAGGT GGTGCTGGCC ATGCCCTACG ACACCCCAGT GCCCGGCTAC AAGAACAACA 
     1741 CCGTCAACAC CATGCGGCTG TGGTCCGCCA AGGCTCCCAA CGACTTCAAG CTGCAGGACT 
     1801 TCAACGTGGG AGACTACATC GAGGCGGTCC TGGACCGGAA CTTGGCTGAG AACATCTCCA 
     1861 GGGTCCTGTA TCCAAATGAT AACTTCTTTG AGGGGAAGGA GCTGCGGCTG AAGCAGGAGT 
     1921 ACTTCGTGGT GGCCGCCACG CTCCAGGACA TCATCCGCCG CTTCAAGTCG TCCAAGTTCG 
     1981 GCTGCCGGGA CCCTGTGAGA ACCTGTTTCG AGACGTTCCC AGACAAGGTG GCCATCCAGC 
     2041 TGAACGACAC CCACCCCGCC CTCTCCATCC CTGAGCTCAT GCGGATCCTG GTGGACGTGG 
     2101 AGAAGGTGGA CTGGGACAAG GCCTGGGAAA TCACGAAGAA GACCTGTGCA TACACCAACC 
     2161 ACACTGTGCT GCCTGAGGCC TTGGAGCGCT GGCCCGTGTC CATGTTTGAG AAGCTGCTGC 
     2221 CGCGGCACCT GGAGATAATC TATGCCATCA ACCAGCGGCA CCTGGACCAC GTGGCCGCGC 
     2281 TGTTTCCCGG CGATGTGGAC CGCCTGCGCA GGATGTCTGT GATCGAGGAG GGGGACTGCA 
     2341 AGCGGATCAA CATGGCCCAC CTGTGTGTGA TTGGGTCCCA TGCTGTCAAT GGTGTGGCGA 
     2401 GGATCCACTC GGAGATCGTG AAACAGTCGG TCTTTAAGGA TTTTTATGAA CTGGAGCCAG 
     2461 AGAAGTTCCA GAATAAGACC AATGGCATCA CCCCCCGCCG GTGGCTGCTG CTGTGCAACC 
     2521 CGGGGCTGGC CGATACCATC GTGGAGAAAA TTGGGGAGGA GTTCCTGACT GACCTGAGCC 
     2581 AGCTGAAGAA GCTGCTGCCG CTGGTCAGTG ACGAGGTGTT CATCAGGGAC GTGGCCAAGG 
     2641 TCAAACAGGA GAACAAGCTC AAGTTCTCGG CCTTCCTGGA GAAGGAGTAC AAGGTGAAGA 
     2701 TCAACCCCTC CTCCATGTTC GATGTGCATG TGAAGAGGAT CCACGAGTAC AAGCGGCAGC 
     2761 TGCTCAACTG CCTGCACGTC GTCACCCTGT ACAATCGAAT CAAGAGAGAC CCGGCCAAGG 
     2821 CTTTTGTGCC CAGGACTGTT ATGATTGGGG GCAAGGCAGC GCCCGGTTAC CACATGGCCA 
     2881 AGCTGATCAT CAAGTTGGTC ACCTCCATCG GCGACGTCGT CAATCATGAC CCAGTTGTGG 
     2941 GTGACAGGTT GAAAGTGATC TTCCTGGAGA ACTACCGTGT GTCCTTGGCT GAGAAAGTGA 
     3001 TCCCGGCCGC TGATCTGTCG CAGCAGATCT CCACTGCAGG CACCGAGGCC TCAGGCACAG 
     3061 GCAACATGAA GTTCATGCTC AACGGGGCCC TCACCATCGG CACCATGGAC GGCGCCAACG 
     3121 TGGAGATGGC CGAGGAGGCC GGGGCCGAGA ACCTCTTCAT CTTCGGCCTG CGGGTGGAGG 
     3181 ATGTCGAGGC CTTGGACCGG AAAGGGTACA ATGCCAGGGA GTACTACGAC CACCTGCCCG 
     3241 AGCTGAAGCA GGCCGTGGAC CAGATCAGCA GTGGCTTTTT TTCTCCCAAG GAGCCAGACT 
     3301 GCTTCAAGGA CATCGTGAAC ATGCTGATGC ACCATGACAG GTTCAAGGTG TTTGCAGACT 
     3361 ATGAAGCCTA CATGCAGTGC CAGGCACAGG TGGACCAGCT GTACCGGAAC CCCAAGGAGT 
     3421 GGACCAAGAA GGTCATCAGG AACATCGCCT GCTCGGGCAA GTTCTCCAGT GACCGGACCA 
     3481 TCACGGAGTA TGCACGGGAG ATCTGGGGTG TGGAGCCCTC CGACCTGCAG ATCCCGCCCC 
     3541 CCAACATCCC CCGGGACAGC GGACCGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3601 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3661 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3721 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3781 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3841 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     3901 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     3961 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4021 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4081 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4141 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4201 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4261 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4321 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4381 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4441 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4501 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4561 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4621 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4681 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4741 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4801 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4861 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4921 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     4981 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5041 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5101 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5161 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5221 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5281 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5341 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5401 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5461 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5521 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5581 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5641 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5701 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5761 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5821 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     5881 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     5941 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6001 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6061 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6121 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6181 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6241 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6301 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6361 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6421 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6481 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6541 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6601 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6661 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6721 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6781 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6841 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     6901 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     6961 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7021 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7081 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7141 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7201 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7261 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7321 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7381 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7441 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7501 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7561 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7621 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7681 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7741 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7801 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7861 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     7921 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     7981 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8041 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8101 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8161 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8221 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8281 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8341 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8401 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8461 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8521 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8581 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8641 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8701 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8761 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8821 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     8881 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     8941 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9001 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9061 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//