LOCUS       RG200527                9383 bp    DNA     circular  SYN 
DEFINITION  PRPF6 (tGFP-tagged) - Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6)
ACCESSION   NM_012469
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3864..4568 
                     /label="GFP"  
     misc_feature    4615..5200 
                     /label="PolyA signal"  
     misc_feature    7292..7937 
                     /label="ColE1"  
     misc_feature    5654..6448 
                     /label="Neo"  
     misc_feature    5285..5587 
                     /label="SV40 ori"  
     misc_feature    7952..8812 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3843..3849 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3843 
                     /label="PRPF6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACAAGAAG AAGAAACCGT TCCTAGGGAT GCCCGCGCCC CTCGGCTACG 
     1081 TGCCGGGGCT GGGCCGGGGC GCCACTGGCT TCACCACGCG GTCAGACATT GGGCCCGCCC 
     1141 GTGATGCAAA TGACCCTGTG GATGATCGCC ATGCACCCCC AGGCAAGAGA ACCGTTGGGG 
     1201 ACCAGATGAA GAAAAATCAG GCTGCTGACG ATGACGACGA GGATCTAAAT GACACCAATT 
     1261 ACGATGAGTT TAATGGCTAT GCTGGGAGCC TCTTCTCAAG TGGACCCTAC GAGAAAGATG 
     1321 ATGAGGAAGC AGATGCTATC TATGCAGCCC TGGATAAAAG GATGGATGAA AGAAGAAAAG 
     1381 AAAGACGGGA GCAAAGGGAG AAAGAAGAAA TAGAGAAATA TCGTATGGAA CGCCCCAAAA 
     1441 TCCAACAGCA GTTCTCAGAC CTCAAGAGGA AGTTGGCAGA AGTCACAGAA GAAGAGTGGC 
     1501 TGAGCATCCC CGAGGTTGGC GATGCCAGAA ATAAACGTCA GCGGAACCCA CGCTATGAGA 
     1561 AGCTGACCCC TGTTCCTGAC AGTTTCTTTG CCAAACATTT ACAGACCGGA GAGAACCATA 
     1621 CCTCAGTGGA TCCCCGACAA ACTCAATTTG GAGGTCTTAA CACACCCTAT CCAGGTGGAC 
     1681 TAAACACTCC ATACCCAGGT GGAATGACGC CAGGACTGAT GACACCTGGC ACAGGTGAGC 
     1741 TGGACATGAG GAAGATTGGC CAAGCGAGGA ACACTCTGAT GGACATGAGG CTGAGCCAGG 
     1801 TGTCTGACTC CGTGAGTGGA CAGACCGTCG TTGACCCCAA AGGCTACCTG ACGGATTTAA 
     1861 ATTCCATGAT CCCGACACAC GGAGGAGACA TCAATGATAT CAAGAAGGCG CGACTGCTCC 
     1921 TCAAGTCTGT TCGGGAGACG AACCCTCATC ACCCGCCAGC CTGGATTGCA TCAGCCCGCC 
     1981 TGGAAGAAGT CACTGGGAAG CTACAAGTAG CTCGGAACCT TATCATGAAG GGGACGGAGA 
     2041 TGTGCCCCAA GAGTGAAGAT GTCTGGCTGG AAGCAGCCAG GTTGCAGCCT GGGGACACAG 
     2101 CCAAGGCCGT GGTAGCCCAA GCTGTCCGTC ATCTCCCACA GTCTGTCAGG ATTTACATCA 
     2161 GAGCCGCAGA GCTGGAAACG GACATTCGTG CAAAGAAGCG GGTTCTTCGG AAAGCCCTCG 
     2221 AGCATGTTCC AAACTCGGTT CGCTTGTGGA AAGCAGCCGT TGAGCTGGAA GAACCTGAAG 
     2281 ATGCTAGAAT CATGCTGAGC CGAGCTGTGG AGTGCTGCCC CACCAGCGTG GAGCTCTGGC 
     2341 TTGCTCTGGC AAGGCTGGAG ACCTATGAAA ATGCCCGCAA GGTCTTGAAC AAGGCGCGGG 
     2401 AGAACATTCC TACAGACCGA CATATCTGGA TCACGGCTGC TAAGCTGGAG GAAGCCAATG 
     2461 GGAACACGCA GATGGTGGAG AAGATCATCG ACCGAGCCAT CACCTCGCTG CGGGCCAACG 
     2521 GTGTGGAGAT CAACCGTGAG CAGTGGATCC AGGATGCCGA GGAATGTGAC AGGGCTGGGA 
     2581 GTGTGGCCAC CTGCCAGGCC GTCATGCGTG CCGTGATTGG GATTGGGATT GAGGAGGAAG 
     2641 ATCGGAAGCA TACCTGGATG GAGGATGCTG ACAGTTGTGT AGCCCACAAT GCCCTGGAGT 
     2701 GTGCACGAGC CATCTACGCC TACGCCCTGC AGGTGTTCCC CAGCAAGAAG AGTGTGTGGC 
     2761 TGCGCGCCGC GTACTTCGAG AAGAACCATG GCACTCGGGA GTCCCTGGAA GCACTCCTGC 
     2821 AGAGGGCTGT GGCCCACTGC CCCAAAGCAG AGGTGCTGTG GCTCATGGGC GCCAAGTCCA 
     2881 AGTGGCTGGC AGGGGATGTG CCTGCAGCAA GGAGCATCCT GGCCCTGGCC TTCCAGGCCA 
     2941 ACCCCAACAG TGAGGAGATC TGGCTGGCAG CCGTGAAGCT GGAGTCCGAG AATGATGAGT 
     3001 ACGAGCGGGC CCGGAGGCTG CTGGCCAAGG CGCGGAGCAG TGCCCCCACC GCCCGGGTGT 
     3061 TCATGAAGTC TGTGAAGCTG GAGTGGGTGC AAGACAACAT CAGGGCAGCC CAAGATCTGT 
     3121 GCGAGGAGGC CCTGCGGCAC TATGAGGACT TCCCCAAGCT GTGGATGATG AAGGGGCAGA 
     3181 TCGAGGAGCA GAAGGAGATG ATGGAGAAGG CGCGGGAAGC CTATAACCAG GGGTTGAAGA 
     3241 AGTGTCCCCA CTCCACACCC CTGTGGCTTT TGCTCTCTCG GCTGGAGGAG AAGATTGGGC 
     3301 AGCTTACTCG AGCACGGGCC ATTTTGGAAA AGTCTCGTCT GAAGAACCCA AAGAACCCTG 
     3361 GGCTGTGGTT GGAGTCCGTG CGGCTGGAGT ACCGTGCGGG GCTGAAGAAC ATCGCAAATA 
     3421 CACTCATGGC CAAGGCGCTG CAGGAGTGCC CCAACTCCGG TATCCTGTGG TCTGAGGCCA 
     3481 TCTTCCTCGA GGCAAGGCCC CAGAGGAGGA CCAAGAGCGT GGATGCCCTG AAGAAGTGTG 
     3541 AGCATGACCC CCATGTGCTC CTGGCCGTGG CCAAGCTGTT TTGGAGTCAG CGGAAGATCA 
     3601 CCAAGGCCAG GGAGTGGTTC CACCGCACTG TGAAGATTGA CTCGGACCTG GGGGATGCCT 
     3661 GGGCCTTCTT CTACAAGTTT GAGCTGCAGC ATGGCACTGA GGAGCAGCAG GAGGAGGTGA 
     3721 GGAAGCGCTG TGAGAGTGCA GAGCCTCGGC ATGGGGAGCT GTGGTGCGCC GTGTCCAAGG 
     3781 ACATCGCCAA CTGGCAGAAG AAGATCGGGG ACATCCTTAG GCTGGTGGCC GGCCGCATCA 
     3841 AGAACACCTT CACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3901 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3961 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     4021 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     4081 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4141 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4201 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4261 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4321 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4381 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4441 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4501 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4561 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4621 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4681 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4741 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4801 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4861 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4921 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4981 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5041 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5101 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5161 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5221 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5281 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5341 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5401 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5461 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5521 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5581 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5641 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5701 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5761 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5821 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5881 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5941 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     6001 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     6061 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6121 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6181 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6241 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6301 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6361 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6421 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6481 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6541 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6601 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6661 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6721 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6781 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6841 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6901 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6961 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     7021 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     7081 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7141 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7201 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7261 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7321 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7381 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7441 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7501 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7561 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7621 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7681 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7741 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7801 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7861 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7921 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7981 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     8041 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     8101 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8161 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8221 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8281 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8341 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8401 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8461 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8521 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8581 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8641 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8701 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8761 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8821 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8881 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8941 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     9001 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     9061 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9121 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9181 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9241 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9301 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9361 AACAAAAATT TAACGCGAAT TTT
//