LOCUS       RG200477                8510 bp    DNA     circular  SYN 
DEFINITION  FOXO1 (tGFP-tagged) - Human forkhead box O1 (FOXO1)
ACCESSION   NM_002015
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2991..3695 
                     /label="GFP"  
     misc_feature    3742..4327 
                     /label="PolyA signal"  
     misc_feature    6419..7064 
                     /label="ColE1"  
     misc_feature    4781..5575 
                     /label="Neo"  
     misc_feature    4412..4714 
                     /label="SV40 ori"  
     misc_feature    7079..7939 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2985..2991 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2985 
                     /label="FOXO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGGCG CCTCAGGTGG TGGAGATCGA CCCGGACTTC GAGCCGCTGC 
     1081 CCCGGCCGCG CTCGTGCACC TGGCCGCTGC CCAGGCCGGA GTTTAGCCAG TCCAACTCGG 
     1141 CCACCTCCAG CCCGGCGCCG TCGGGCAGCG CGGCTGCCAA CCCCGACGCC GCGGCGGGCC 
     1201 TGCCCTCGGC CTCGGCTGCC GCTGTCAGCG CCGACTTCAT GAGCAACCTG AGCTTGCTGG 
     1261 AGGAGAGCGA GGACTTCCCG CAGGCGCCCG GCTCCGTGGC GGCGGCGGTG GCGGCGGCGG 
     1321 CCGCCGCGGC CGCCACCGGG GGGCTGTGCG GGGACTTCCA GGGCCCGGAG GCGGGCTGCC 
     1381 TGCACCCAGC GCCACCGCAG CCCCCGCCGC CCGGGCCGCT GTCGCAGCAC CCGCCGGTGC 
     1441 CCCCCGCCGC CGCTGGGCCG CTCGCGGGGC AGCCGCGCAA GAGCAGCTCG TCCCGCCGCA 
     1501 ACGCGTGGGG CAACCTGTCC TACGCCGACC TCATCACCAA GGCCATCGAG AGCTCGGCGG 
     1561 AGAAGCGGCT CACGCTGTCG CAGATCTACG AGTGGATGGT CAAGAGCGTG CCCTACTTCA 
     1621 AGGATAAGGG TGACAGCAAC AGCTCGGCGG GCTGGAAGAA TTCAATTCGT CATAATCTGT 
     1681 CCCTACACAG CAAGTTCATT CGTGTGCAGA ATGAAGGAAC TGGAAAAAGT TCTTGGTGGA 
     1741 TGCTCAATCC AGAGGGTGGC AAGAGCGGGA AATCTCCTAG GAGAAGAGCT GCATCCATGG 
     1801 ACAACAACAG TAAATTTGCT AAGAGCCGAA GCCGAGCTGC CAAGAAGAAA GCATCTCTCC 
     1861 AGTCTGGCCA GGAGGGTGCT GGGGACAGCC CTGGATCACA GTTTTCCAAA TGGCCTGCAA 
     1921 GCCCTGGCTC TCACAGCAAT GATGACTTTG ATAACTGGAG TACATTTCGC CCTCGAACTA 
     1981 GCTCAAATGC TAGTACTATT AGTGGGAGAC TCTCACCCAT TATGACCGAA CAGGATGATC 
     2041 TTGGAGAAGG GGATGTGCAT TCTATGGTGT ACCCGCCATC TGCCGCAAAG ATGGCCTCTA 
     2101 CTTTACCCAG TCTGTCTGAG ATAAGCAATC CCGAAAACAT GGAAAATCTT TTGGATAATC 
     2161 TCAACCTTCT CTCATCACCA ACATCATTAA CTGTTTCGAC CCAGTCCTCA CCTGGCACCA 
     2221 TGATGCAGCA GACGCCGTGC TACTCGTTTG CGCCACCAAA CACCAGTTTG AATTCACCCA 
     2281 GCCCAAACTA CCAAAAATAT ACATATGGCC AATCCAGCAT GAGCCCTTTG CCCCAGATGC 
     2341 CTATACAAAC ACTTCAGGAC AATAAGTCGA GTTATGGAGG TATGAGTCAG TATAACTGTG 
     2401 CGCCTGGACT CTTGAAGGAG TTGCTGACTT CTGACTCTCC TCCCCATAAT GACATTATGA 
     2461 CACCAGTTGA TCCTGGGGTA GCCCAGCCCA ACAGCCGGGT TCTGGGCCAG AACGTCATGA 
     2521 TGGGCCCTAA TTCGGTCATG TCAACCTATG GCAGCCAGGC ATCTCATAAC AAAATGATGA 
     2581 ATCCCAGCTC CCATACCCAC CCTGGACATG CTCAGCAGAC ATCTGCAGTT AACGGGCGTC 
     2641 CCCTGCCCCA CACGGTAAGC ACCATGCCCC ACACCTCGGG TATGAACCGC CTGACCCAAG 
     2701 TGAAGACACC TGTACAAGTG CCTCTGCCCC ACCCCATGCA GATGAGTGCC CTGGGGGGCT 
     2761 ACTCCTCCGT GAGCAGCTGC AATGGCTATG GCAGAATGGG CCTTCTCCAC CAGGAGAAGC 
     2821 TCCCAAGTGA CTTGGATGGC ATGTTCATTG AGCGCTTAGA CTGTGACATG GAATCCATCA 
     2881 TTCGGAATGA CCTCATGGAT GGAGATACAT TGGATTTTAA CTTTGACAAT GTGTTGCCCA 
     2941 ACCAAAGCTT CCCACACAGT GTCAAGACAA CGACACATAG CTGGGTGTCA GGCCTCGAGA 
     3001 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     3061 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     3121 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     3181 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     3241 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     3301 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     3361 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     3421 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     3481 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     3541 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     3601 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     3661 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     3721 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3781 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3841 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3901 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3961 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4021 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4081 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4141 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4201 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4261 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4321 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4381 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4441 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     4501 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     4561 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     4621 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     4681 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     4741 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     4801 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     4861 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     4921 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     4981 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     5041 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     5101 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     5161 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     5221 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     5281 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     5341 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     5401 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     5461 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     5521 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     5581 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     5641 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     5701 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     5761 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     5821 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     5881 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     5941 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6001 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     6061 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     6121 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     6181 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     6241 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     6301 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     6361 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     6421 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     6481 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     6541 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     6601 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     6661 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     6721 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     6781 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     6841 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     6901 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     6961 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7021 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     7081 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     7141 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     7201 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     7261 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     7321 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     7381 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     7441 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     7501 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     7561 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     7621 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     7681 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     7741 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     7801 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     7861 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     7921 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     7981 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     8041 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     8101 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     8161 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     8221 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     8281 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     8341 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     8401 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     8461 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//