LOCUS       RG200445                9119 bp    DNA     circular  SYN 
DEFINITION  PDE6B (tGFP-tagged) - Human phosphodiesterase 6B, cGMP-specific, rod, beta (PDE6B), transcript variant 1
ACCESSION   NM_000283
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3600..4304 
                     /label="GFP"  
     misc_feature    4351..4936 
                     /label="PolyA signal"  
     misc_feature    7028..7673 
                     /label="ColE1"  
     misc_feature    5390..6184 
                     /label="Neo"  
     misc_feature    5021..5323 
                     /label="SV40 ori"  
     misc_feature    7688..8548 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3579..3585 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3579 
                     /label="PDE6B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCTCAGT GAGGAGCAGG CCCGGAGCTT TCTGGACCAG AACCCCGATT 
     1081 TTGCCCGCCA GTACTTTGGG AAGAAACTGA GCCCTGAGAA TGTGGCCGCG GCCTGCGAGG 
     1141 ACGGGTGCCC GCCGGACTGC GACAGCCTCC GGGACCTCTG CCAGGTGGAG GAGAGCACGG 
     1201 CGCTGCTGGA GCTGGTGCAG GATATGCAGG AGAGCATCAA CATGGAGCGC GTGGTCTTCA 
     1261 AGGTCCTGCG GCGCCTCTGC ACCCTCCTGC AGGCCGACCG CTGCAGCCTC TTCATGTACC 
     1321 GCCAGCGCAA CGGCGTGGCC GAGCTGGCCA CCAGGCTTTT CAGCGTGCAG CCGGACAGCG 
     1381 TCCTGGAGGA CTGCCTGGTG CCCCCCGACT CCGAGATCGT CTTCCCACTG GACATCGGGG 
     1441 TCGTGGGCCA CGTGGCTCAG ACCAAAAAGA TGGTGAACGT CGAGGACGTG GCCGAGTGCC 
     1501 CTCACTTCAG CTCATTTGCT GACGAGCTCA CTGACTACAA GACAAAGAAT ATGCTGGCCA 
     1561 CACCCATCAT GAATGGCAAA GACGTCGTGG CGGTGATCAT GGCAGTGAAC AAGCTCAACG 
     1621 GCCCATTCTT CACCAGCGAA GACGAAGATG TGTTCTTGAA GTACCTGAAT TTTGCCACGT 
     1681 TGTACCTGAA GATCTATCAC CTGAGCTACC TCCACAACTG CGAGACGCGC CGCGGCCAGG 
     1741 TGCTGCTGTG GTCGGCCAAC AAGGTGTTTG AGGAGCTGAC GGACATCGAG AGGCAGTTCC 
     1801 ACAAGGCCTT CTACACGGTG CGGGCCTACC TCAACTGCGA GCGGTACTCC GTGGGCCTCC 
     1861 TGGACATGAC CAAGGAGAAG GAATTTTTTG ACGTGTGGTC TGTGCTGATG GGAGAGTCCC 
     1921 AGCCGTACTC GGGCCCACGC ACGCCTGATG GCCGGGAAAT TGTCTTCTAC AAAGTGATCG 
     1981 ACTACATCCT CCACGGCAAG GAGGAGATCA AGGTCATTCC CACACCCTCA GCCGATCACT 
     2041 GGGCCCTGGC CAGCGGCCTT CCAAGCTACG TGGCAGAAAG CGGCTTTATT TGTAACATCA 
     2101 TGAATGCTTC CGCTGACGAA ATGTTCAAAT TTCAGGAAGG GGCCCTGGAC GACTCCGGGT 
     2161 GGCTCATCAA GAATGTGCTG TCCATGCCCA TCGTCAACAA GAAGGAGGAG ATTGTGGGAG 
     2221 TCGCCACATT TTACAACAGG AAAGACGGGA AGCCCTTTGA CGAACAGGAC GAGGTTCTCA 
     2281 TGGAGTCCCT GACACAGTTC CTGGGCTGGT CAGTGATGAA CACCGACACC TACGACAAGA 
     2341 TGAACAAGCT GGAGAACCGC AAGGACATCG CACAGGACAT GGTCCTTTAC CACGTGAAGT 
     2401 GCGACAGGGA CGAGATCCAG CTCATCCTGC CAACCAGAGC GCGCCTGGGG AAGGAGCCTG 
     2461 CTGACTGCGA TGAGGACGAG CTGGGCGAAA TCCTGAAGGA GGAGCTGCCA GGGCCCACCA 
     2521 CATTTGACAT CTACGAATTC CACTTCTCTG ACCTGGAGTG CACCGAACTG GACCTGGTCA 
     2581 AATGTGGCAT CCAGATGTAC TACGAGCTGG GCGTGGTCCG AAAGTTCCAG ATCCCCCAGG 
     2641 AGGTCCTGGT GCGGTTCCTG TTCTCCATCA GCAAAGGGTA CCGGAGAATC ACCTACCACA 
     2701 ACTGGCGCCA CGGCTTCAAC GTGGCCCAGA CGATGTTCAC GCTGCTCATG ACCGGCAAAC 
     2761 TGAAGAGCTA CTACACGGAC CTGGAGGCCT TCGCCATGGT GACAGCCGGC CTGTGCCATG 
     2821 ACATCGACCA CCGCGGCACC AACAACCTGT ACCAGATGAA GTCCCAGAAC CCCTTGGCTA 
     2881 AGCTCCACGG CTCCTCGATT TTGGAGCGGC ACCACCTGGA GTTTGGGAAG TTCCTGCTCT 
     2941 CGGAGGAGAC CCTGAACATC TACCAGAACC TGAACCGGCG GCAGCACGAT CACGTGATCC 
     3001 ACCTGATGGA CATCGCCATC ATCGCCACGG ACCTGGCCCT GTACTTCAAG AAGAGAGCGA 
     3061 TGTTTCAGAA GATCGTGGAT GAGTCCAAGA ACTACCAGGA CAAGAAGAGC TGGGTGGAGT 
     3121 ACCTGTCCCT GGAGACGACC CGGAAGGAGA TCGTCATGGC CATGATGATG ACAGCCTGCG 
     3181 ACCTGTCTGC CATCACCAAG CCCTGGGAAG TCCAGAGCAA GGTCGCACTT CTCGTGGCTG 
     3241 CTGAGTTCTG GGAGCAAGGT GACTTGGAAA GGACAGTCTT GGATCAGCAG CCCATTCCTA 
     3301 TGATGGACCG GAACAAGGCG GCCGAGCTCC CCAAGCTGCA AGTGGGCTTC ATCGACTTCG 
     3361 TGTGCACATT CGTGTACAAG GAGTTCTCTC GTTTCCACGA AGAGATCCTG CCCATGTTCG 
     3421 ACCGACTGCA GAACAATAGG AAAGAGTGGA AGGCGCTGGC TGATGAGTAT GAGGCCAAAG 
     3481 TGAAGGCTCT GGAGGAGAAG GAGGAGGAGG AGAGGGTGGC AGCCAAGAAA GGCACAGAAA 
     3541 TTTGCAATGG CGGCCCAGCA CCCAAGTCTT CAACCTGCTG TATCCTGACG CGTACGCGGC 
     3601 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3661 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3721 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3781 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     3841 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     3901 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     3961 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4021 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4081 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4141 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4201 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4261 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4321 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4381 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4441 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4501 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4561 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4621 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4681 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4741 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4801 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4861 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4921 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4981 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5041 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5101 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5161 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5221 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5281 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5341 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5401 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5461 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5521 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5581 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5641 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5701 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5761 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     5821 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     5881 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     5941 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6001 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6061 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6121 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6181 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6241 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6301 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6361 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6421 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6481 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6541 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6601 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6661 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6721 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6781 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     6841 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     6901 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     6961 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7021 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7081 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7141 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7201 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7261 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7321 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7381 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7441 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7501 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7561 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7621 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7681 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7741 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7801 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     7861 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     7921 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     7981 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8041 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8101 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8161 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8221 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8281 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8341 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8401 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8461 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8521 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8581 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8641 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8701 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8761 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     8821 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     8881 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     8941 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9001 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9061 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//