LOCUS       RG200093                9131 bp    DNA     circular  SYN 
DEFINITION  TTK (tGFP-tagged) - Human TTK protein kinase (TTK), transcript variant 1
ACCESSION   NM_003318
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3612..4316 
                     /label="GFP"  
     misc_feature    4363..4948 
                     /label="PolyA signal"  
     misc_feature    7040..7685 
                     /label="ColE1"  
     misc_feature    5402..6196 
                     /label="Neo"  
     misc_feature    5033..5335 
                     /label="SV40 ori"  
     misc_feature    7700..8560 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3591..3597 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3591 
                     /label="TTK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATCCGAG GATTTAAGTG GCAGAGAATT GACAATTGAT TCCATAATGA 
     1081 ACAAAGTGAG AGACATTAAA AATAAGTTTA AAAATGAAGA CCTTACTGAT GAACTAAGCT 
     1141 TGAATAAAAT TTCTGCTGAT ACTACAGATA ACTCGGGAAC TGTTAACCAA ATTATGATGA 
     1201 TGGCAAACAA CCCAGAGGAC TGGTTGAGTT TGTTGCTCAA ACTAGAGAAA AACAGTGTTC 
     1261 CGCTAAGTGA TGCTCTTTTA AATAAATTGA TTGGTCGTTA CAGTCAAGCA ATTGAAGCGC 
     1321 TTCCCCCAGA TAAATATGGC CAAAATGAGA GTTTTGCTAG AATTCAAGTG AGATTTGCTG 
     1381 AATTAAAAGC TATTCAAGAG CCAGATGATG CACGTGACTA CTTTCAAATG GCCAGAGCAA 
     1441 ACTGCAAGAA ATTTGCTTTT GTTCATATAT CTTTTGCACA ATTTGAACTG TCACAAGGTA 
     1501 ATGTCAAAAA AAGTAAACAA CTTCTTCAAA AAGCTGTAGA ACGTGGAGCA GTACCACTAG 
     1561 AAATGCTGGA AATTGCCCTG CGGAATTTAA ACCTCCAAAA AAAGCAGCTG CTTTCAGAGG 
     1621 AGGAAAAGAA GAATTTATCA GCATCTACGG TATTAACTGC CCAAGAATCA TTTTCCGGTT 
     1681 CACTTGGGCA TTTACAGAAT AGGAACAACA GTTGTGATTC CAGAGGACAG ACTACTAAAG 
     1741 CCAGGTTTTT ATATGGAGAG AACATGCCAC CACAAGATGC AGAAATAGGT TACCGGAATT 
     1801 CATTGAGACA AACTAACAAA ACTAAACAGT CATGCCCATT TGGAAGAGTC CCAGTTAACC 
     1861 TTCTAAATAG CCCAGATTGT GATGTGAAGA CAGATGATTC AGTTGTACCT TGTTTTATGA 
     1921 AAAGACAAAC CTCTAGATCA GAATGCCGAG ATTTGGTTGT GCCTGGATCT AAACCAAGTG 
     1981 GAAATGATTC CTGTGAATTA AGAAATTTAA AGTCTGTTCA AAATAGTCAT TTCAAGGAAC 
     2041 CTCTGGTGTC AGATGAAAAG AGTTCTGAAC TTATTATTAC TGATTCAATA ACCCTGAAGA 
     2101 ATAAAACGGA ATCAAGTCTT CTAGCTAAAT TAGAAGAAAC TAAAGAGTAT CAAGAACCAG 
     2161 AGGTTCCAGA GAGTAACCAG AAACAGTGGC AATCTAAGAG AAAGTCAGAG TGTATTAACC 
     2221 AGAATCCTGC TGCATCTTCA AATCACTGGC AGATTCCGGA GTTAGCCCGA AAAGTTAATA 
     2281 CAGAGCAGAA ACATACCACT TTTGAGCAAC CTGTCTTTTC AGTTTCAAAA CAGTCACCAC 
     2341 CAATATCAAC ATCTAAATGG TTTGACCCAA AATCTATTTG TAAGACACCA AGCAGCAATA 
     2401 CCTTGGATGA TTACATGAGC TGTTTTAGAA CTCCAGTTGT AAAGAATGAC TTTCCACCTG 
     2461 CTTGTCAGTT GTCAACACCT TATGGCCAAC CTGCCTGTTT CCAGCAGCAA CAGCATCAAA 
     2521 TACTTGCCAC TCCACTTCAA AATTTACAGG TTTTAGCATC TTCTTCAGCA AATGAATGCA 
     2581 TTTCGGTTAA AGGAAGAATT TATTCCATTT TAAAGCAGAT AGGAAGTGGA GGTTCAAGCA 
     2641 AGGTATTTCA GGTGTTAAAT GAAAAGAAAC AGATATATGC TATAAAATAT GTGAACTTAG 
     2701 AAGAAGCAGA TAACCAAACT CTTGATAGTT ACCGGAACGA AATAGCTTAT TTGAATAAAC 
     2761 TACAACAACA CAGTGATAAG ATCATCCGAC TTTATGATTA TGAAATCACG GACCAGTACA 
     2821 TCTACATGGT AATGGAGTGT GGAAATATTG ATCTTAATAG TTGGCTTAAA AAGAAAAAAT 
     2881 CCATTGATCC ATGGGAACGC AAGAGTTACT GGAAAAATAT GTTAGAGGCA GTTCACACAA 
     2941 TCCATCAACA TGGCATTGTT CACAGTGATC TTAAACCAGC TAACTTTCTG ATAGTTGATG 
     3001 GAATGCTAAA GCTAATTGAT TTTGGGATTG CAAACCAAAT GCAACCAGAT ACAACAAGTG 
     3061 TTGTTAAAGA TTCTCAGGTT GGCACAGTTA ATTATATGCC ACCAGAAGCA ATCAAAGATA 
     3121 TGTCTTCCTC CAGAGAGAAT GGGAAATCTA AGTCAAAGAT AAGCCCCAAA AGTGATGTTT 
     3181 GGTCCTTAGG ATGTATTTTG TACTATATGA CTTACGGGAA AACACCATTT CAGCAGATAA 
     3241 TTAATCAGAT TTCTAAATTA CATGCCATAA TTGATCCTAA TCATGAAATT GAATTTCCCG 
     3301 ATATTCCAGA GAAAGATCTT CAAGATGTGT TAAAGTGTTG TTTAAAAAGG GACCCAAAAC 
     3361 AGAGGATATC CATTCCTGAG CTCCTGGCTC ATCCCTATGT TCAAATTCAA ACTCATCCAG 
     3421 TTAACCAAAT GGCCAAGGGA ACCACTGAAG AAATGAAATA TGTTCTGGGC CAACTTGTTG 
     3481 GTCTGAATTC TCCTAACTCC ATTTTGAAAG CTGCTAAAAC TTTATATGAA CACTATAGTG 
     3541 GTGGTGAAAG TCATAATTCT TCATCCTCCA AGACTTTTGA AAAAAAAAGG GGAAAAAAAA 
     3601 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     3661 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     3721 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     3781 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     3841 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     3901 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     3961 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     4021 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     4081 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     4141 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     4201 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4261 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4321 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4381 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4441 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4501 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4561 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4621 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4681 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4741 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4801 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4861 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4921 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4981 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     5041 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5101 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5161 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     5221 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5281 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5341 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5401 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5461 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5521 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5581 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     5641 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     5701 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     5761 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     5821 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     5881 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     5941 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     6001 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     6061 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     6121 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     6181 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6241 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6301 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6361 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6421 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6481 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6541 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6601 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     6661 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     6721 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     6781 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     6841 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     6901 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     6961 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     7021 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     7081 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     7141 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     7201 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7261 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7321 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7381 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7441 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7501 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7561 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7621 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     7681 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     7741 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     7801 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     7861 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     7921 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     7981 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     8041 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     8101 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     8161 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     8221 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8281 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8341 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8401 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8461 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8521 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8581 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     8641 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     8701 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     8761 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     8821 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     8881 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     8941 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     9001 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     9061 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     9121 ACGCGAATTT T
//