LOCUS       RC240163                9339 bp    DNA     circular  SYN 
DEFINITION  PIK3C2G (myc-DDK-tagged) - Human phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 gamma (PIK3C2G), transcript variant 1
ACCESSION   NM_001288772
VERSION     NM_001288772.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5496..5587 
                     /label="Myc-DDK"  
     misc_feature    5617..6202 
                     /label="PolyA signal"  
     misc_feature    5674..5693 
                     /label="Restriction Enzyme"  
     misc_feature    6478..7150 
                     /label="ColE1"  
     misc_feature    7580..8374 
                     /label="Kan/Neo"  
     misc_feature    8409..8711 
                     /label="SV40 ori"  
     misc_feature    8797..9274 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5478..5484 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5478 
                     /label="PIK3C2G"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATATTCT TGGCAAACGG ATCCAAATCC TAATGAATCA CACGAAAAGC 
     1081 AGTATGAACA CCAAGAATTT CTCTTTGTAA ATCAACCCCA TTCTTCTAGC CAAGTCAGTC 
     1141 TGGGTTTTGA TCAGATAGTA GATGAGATCA GTGGCAAAAT TCCACACTAC GAGAGTGAAA 
     1201 TTGATGAAAA CACCTTTTTT GTGCCCACTG CACCAAAATG GGACTCAACA GGGCATTCAT 
     1261 TAAATGAAGC ACACCAAATA TCCTTGAATG AATTCACTTC TAAAAGCCGT GAACTCTCCT 
     1321 GGCATCAAGT TAGCAAAGCA CCAGCAATTG GTTTTAGTCC TTCTGTGTTA CCAAAACCTC 
     1381 AAAATACGAA TAAAGAATGC TCCTGGGGAA GCCCCATAGG AAAACATCAT GGTGCTGATG 
     1441 ATTCCAGATT CAGTATTTTA GCTCCATCAT TCACAAGTTT GGATAAAATT AATCTAGAGA 
     1501 AAGAATTAGA AAATGAAAAT CATAACTACC ATATAGGATT TGAAAGTAGC ATTCCTCCAA 
     1561 CAAATTCATC CTTCTCAAGT GACTTCATGC CGAAAGAAGA GAATAAAAGG AGTGGACATG 
     1621 TGAACATTGT GGAACCATCT TTGATGCTTT TGAAAGGCTC TCTTCAACCC GGAATGTGGG 
     1681 AAAGTACATG GCAGAAGAAT ATAGAGTCAA TAGGTTGTTC CATTCAGCTA GTGGAAGTAC 
     1741 CTCAAAGCAG CAATACGAGT CTGGCCTCTT TTTGCAACAA AGTAAAAAAA ATCAGAGAAA 
     1801 GATATCATGC AGCTGATGTT AATTTCAATT CTGGGAAGAT CTGGAGCACT ACTACAGCAT 
     1861 TTCCGTATCA GCTCTTTTCT AAGACCAAGT TTAATATACA TATTTTTATT GATAACTCAA 
     1921 CACAACCTCT TCATTTTATG CCATGTGCTA ATTATCTTGT CAAAGATCTA ATTGCAGAAA 
     1981 TTCTGCATTT TTGCACAAAT GACCAGCTAC TCCCCAAAGA TCATATTCTA AGTGTATGTG 
     2041 GCTCTGAAGA ATTTTTACAA AACGACCACT GTTTGGGGAG CCACAAAATG TTTCAAAAAG 
     2101 ATAAATCTGT TATTCAGCTC CACCTGCAGA AAAGTAGGGA AGCTCCAGGA AAGCTATCTC 
     2161 GAAAGCATGA AGAGGACCAC AGTCAGTTTT ATCTGAATCA ACTTCTAGAA TTTATGCATA 
     2221 TTTGGAAAGT ATCCAGACAA TGTCTCTTAA CACTCATCAG AAAATATGAC TTCCACCTGA 
     2281 AATACCTATT GAAAACCCAG GAAAACGTGT ATAATATTAT TGAAGAAGTT AAAAAAATAT 
     2341 GCAGTGTTCT AGGGTGTGTG GAAACCAAAC AAATTACAGA TGCAGTAAAT GAACTAAGTC 
     2401 TAATTCTTCA GAGAAAAGGA GAGAATTTTT ATCAAAGTTC AGAGACTTCA GCAAAAGGCT 
     2461 TGATAGAGAA GGTAACAACT GAACTATCCA CATCCATCTA CCAGCTAATC AATGTCTACT 
     2521 GTAACAGCTT TTATGCAGAT TTTCAGCCTG TAAATGTACC TAGATGCACT TCCTATCTAA 
     2581 ATCCCGGGCT TCCTTCCCAC CTCAGCTTCA CAGTGTATGC AGCACACAAC ATTCCAGAAA 
     2641 CCTGGGTGCA CAGCTACAAA GCGTTTTCTT TTACCTGTTG GCTTACATAT GCTGGAAAGA 
     2701 AGCTGTGCCA AGTGAGAAAC TACAGAAATA TTCCAGACAA GAAATTATTT TTTTTCTTGG 
     2761 TCAACTGGAA TGAAACGATC AATTTTCCCC TTGAAATAAA GTCACTTCCA AGGGAATCCA 
     2821 TGCTCACTGT AAAACTGTTT GGGATTGCCT GTGCAACCAA CAATGCAAAT TTACTGGCGT 
     2881 GGACTTGTCT TCCACTGTTT CCAAAAGAAA AATCCATTCT CGGGTCTATG CTGTTCAGCA 
     2941 TGACATTACA GAGTGAGCCT CCCGTAGAAA TGATAACTCC AGGAGTGTGG GATGTAAGTC 
     3001 AGCCATCCCC GGTGACCCTG CAGATTGATT TTCCAGCTAC TGGGTGGGAG TATATGAAAC 
     3061 CTGATTCTGA AGAGAATAGA AGTAATCTTG AAGAGCCACT AAAGGAGTGT ATAAAACATA 
     3121 TTGCCAGACT TTCACAGAAA CAGACTCCCC TACTACTCTC TGAAGAAAAG AAAAGATATT 
     3181 TATGGTTTTA TCGCTTCTAC TGCAATAATG AAAACTGCTC CCTTCCTTTA GTCCTGGGTA 
     3241 GTGCCCCTGG ATGGGATGAA AGGACTGTTT CAGAAATGCA TACCATTTTG AGAAGATGGA 
     3301 CATTTTCTCA ACCTTTAGAG GCTCTTGGGC TTTTGACTTC CAGTTTTCCA GATCAAGAAA 
     3361 TTCGTAAAGT GGCAGTTCAA CAATTAGACA ACCTCTTGAA TGATGAACTA CTGGAATATC 
     3421 TCCCACAGCT AGTTCAGGCT GTCAAGTTTG AATGGAACCT TGAGAGTCCT TTAGTGCAAC 
     3481 TTCTACTCCA CCGCTCCTTG CAGAGCATCC AGGTTGCCCA TCGTCTTTAC TGGCTGCTAA 
     3541 AAAATGCAGA AAATGAAGCT TATTTTAAAA GCTGGTATCA GAAGCTACTA GCTGCTCTCC 
     3601 AATTCTGTGC AGGTAAAGCC TTGAATGATG AGTTTTCCAA GGAGCAGAAA CTTATCAAAA 
     3661 TTCTGGGAGA TATTGGGGAA AGAGTCAAGT CTGCCAGTGA CCATCAAAGA CAGGAGGTAC 
     3721 TGAAGAAAGA AATTGGCAGA CTAGAAGAGT TCTTTCAAGA TGTAAATACT TGTCATCTTC 
     3781 CTCTGAACCC TGCCCTATGT ATAAAAGGGA TTGATCACGA TGCATGTTCA TATTTTACAT 
     3841 CTAATGCTTT GCCATTGAAG ATTACTTTCA TCAATGCTAA TCCGATGGGC AAAAACATCA 
     3901 GCATTATTTT TAAGGCTGGA GATGATCTTC GTCAGGATAT GCTTGTTCTG CAGCTTATTC 
     3961 AAGTGATGGA CAATATTTGG CTGCAGGAAG GCTTGGATAT GCAAATGATC ATTTATAGAT 
     4021 GTCTATCCAC AGGAAAAGAC CAAGGATTGG TGCAGATGGT ACCTGATGCT GTGACCCTAG 
     4081 CAAAGATTCA TCGCCATTCT GGACTGATAG GACCATTGAA AGAAAATACA ATTAAAAAGT 
     4141 GGTTCAGTCA GCACAACCAC TTAAAGGCAG ATTATGAAAA GGCCTTGAGG AACTTTTTCT 
     4201 ACTCCTGTGC TGGCTGGTGT GTGGTAACAT TCATCCTGGG AGTATGTGAC CGTCACAATG 
     4261 ATAATATCAT GCTGACAAAG TCGGGCCACA TGTTTCATAT TGACTTTGGA AAATTCTTAG 
     4321 GTCATGCACA AACATTTGGA GGGATAAAAA GGGACCGAGC TCCTTTCATT TTTACTTCAG 
     4381 AGATGGAATA CTTTATTACA GAGGGTGGGA AAAACCCACA GCATTTTCAA GATTTTGTGG 
     4441 AACTTTGCTG TCGTGCTTAT AATATTATCA GAAAGCACAG CCAACTGCTC TTGAACCTGC 
     4501 TGGAAATGAT GCTGTATGCA GGACTGCCTG AGCTAAGTGG AATTCAAGAC CTGAAATATG 
     4561 TGTATAATAA TCTTCGTCCA CAAGACACAG ACCTGGAAGC AACAAGTCAT TTTACCAAGA 
     4621 AAATAAAGGA AAGTCTGGAG TGTTTCCCTG TTAAATTGAA TAACTTGATC CACACACTTG 
     4681 CACAAATGTC AGCCATAAGC CCTGCCAAAT CTACTTCACA GACTTTTCCT CAGGAATCCT 
     4741 GTTTGCTGAG TACAACTAGG TCGATTGAAA GAGCAACAAT TTTAGGGTTC AGCAAGAAAT 
     4801 CCAGTAATCT GTATCTGATC CAGGTGACAC ACAGCAACAA CGAAACAAGC CTGACAGAAA 
     4861 AATCATTTGA GCAGTTTTCA AAACTTCACA GCCAACTTCA GAAGCAGTTT GCATCACTGA 
     4921 CTCTCCCAGA GTTTCCTCAT TGGTGGCACC TACCTTTTAC AAATTCAGAT CACAGAAGAT 
     4981 TCAGAGATCT AAATCATTAC ATGGAACAGA TATTAAATGT ATCACATGAA GTTACAAACA 
     5041 GTGATTGTGT ACTTAGCTTT TTCCTCTCTG AGGCTGTGCA ACAAACAGTT GAAGAATCAT 
     5101 CACCTGTGTA CCTAGGTGAG AAGTTTCCAG ACAAGAAGCC TAAGGTGCAG TTAGTCATAT 
     5161 CCTACGAGGA TGTGAAGCTG ACCATACTAG TGAAACACAT GAAAAACATT CATCTCCCAG 
     5221 ATGGCTCTGC GCCCAGTGCA CATGTTGAAT TTTATCTTTT ACCATATCCC AGTGAAGTTC 
     5281 GTAGGAGGAA AACAAAATCT GTTCCAAAAT GTACGGACCC CACTTACAAT GAAATTGTAG 
     5341 TATATGATGA AGTCACAGAG CTCCAAGGAC ATGTCTTAAT GCTTATTGTG AAGAGTAAAA 
     5401 CTGTATTTGT GGGAGCAATT AACATCCGAC TCTGTAGTGT CCCACTCGAT AAAGAAAAAT 
     5461 GGTATCCATT AGGAAACAGT ATAATTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5521 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5581 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5641 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5701 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5761 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5821 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5881 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5941 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     6001 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     6061 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6121 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6181 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6241 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6301 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6361 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6421 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6481 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6541 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6601 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6661 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6721 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6781 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6841 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6901 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6961 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     7021 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     7081 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7141 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7201 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7261 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7321 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7381 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7441 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7501 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7561 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7621 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7681 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7741 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7801 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7861 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7921 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7981 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     8041 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     8101 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8161 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8221 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8281 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8341 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8401 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8461 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8521 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8581 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8701 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8761 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8821 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8881 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8941 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     9001 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     9061 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9121 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9181 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9241 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9301 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//