LOCUS       RC240155                9261 bp    DNA     circular  SYN 
DEFINITION  GAPVD1 (myc-DDK-tagged) - Human GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant 2
ACCESSION   NM_001282680
VERSION     NM_001282680.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5418..5509 
                     /label="Myc-DDK"  
     misc_feature    5539..6124 
                     /label="PolyA signal"  
     misc_feature    5596..5615 
                     /label="Restriction Enzyme"  
     misc_feature    6400..7072 
                     /label="ColE1"  
     misc_feature    7502..8296 
                     /label="Kan/Neo"  
     misc_feature    8331..8633 
                     /label="SV40 ori"  
     misc_feature    8719..9196 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5400..5406 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5400 
                     /label="GAPVD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAAACTA GATATTCATA CTCTGGCTCA TCACCTCAAG CAGGAACGCT 
     1081 TATATGTAAA CTCTGAGAAA CAGCTCATTC AGAGGCTCAA TGCAGATGTA CTTAAGACAG 
     1141 CTGAAAAGTT GTATCGTACA GCATGGATTG CGAAGCAACA GAGAATCAAT TTGGATCGGC 
     1201 TTATCATAAC CAGTGCTGAA GCTTCCCCTG CTGAATGTTG CCAACATGCC AAAATTTTGG 
     1261 AAGATACACA ATTTGTTGAT GGGTATAAGC AATTGGGATT TCAGGAGACT GCTTATGGAG 
     1321 AATTCTTGAG TCGATTGAGG GAAAATCCTC GTCTTATTGC CTCCTCTTTG GTTGCTGGAG 
     1381 AGAAACTTAA TCAGGAGAAC ACACAAAGTG TTATTTACAC AGTTTTTACC TCCCTGTATG 
     1441 GCAATTGCAT CATGCAAGAA GATGAAAGCT ACCTCCTTCA GGTTTTGCGA TACTTGATTG 
     1501 AATTTGAACT TAAAGAAAGT GACAACCCTA GGCGACTTTT GAGGAGAGGA ACTTGTGCCT 
     1561 TCAGCATCTT ATTTAAACTT TTTTCTGAAG GACTGTTTTC TGCCAAACTT TTCCTCACAG 
     1621 CCACTTTACA TGAGCCAATT ATGCAACTGC TTGTTGAAGA TGAAGATCAC CTGGAAACAG 
     1681 ATCCAAACAA GCTAATTGAG AGGTTCTCTC CATCTCAGCA GGAAAAACTC TTTGGAGAGA 
     1741 AGGGCTCAGA TAGATTCAGG CAAAAAGTTC AAGAAATGGT GGAGTCCAAT GAAGCAAAGC 
     1801 TAGTGGCTTT GGTGAACAAA TTTATTGGTT ATCTCAAACA GAACACATAT TGTTTTCCAC 
     1861 ATAGTTTAAG GTGGATCGTG TCTCAGATGT ACAAAACCCT CTCCTGTGTA GATAGGCTGG 
     1921 AAGTTGGGGA GGTCAGGGCA ATGTGTACTG ATCTCCTGTT GGCCTGCTTC ATTTGTCCTG 
     1981 CAGTTGTCAA TCCAGAACAA TATGGAATAA TTTCCGATGC TCCTATTAAT GAAGTAGCAC 
     2041 GATTTAATCT GATGCAGGTA GGCCGCCTTT TGCAGCAGTT AGCAATGACT GGCTCTGAAG 
     2101 AGGGAGATCC CCGAACAAAG AGCAGCCTTG GAAAGTTTGA CAAAAGCTGT GTTGCCGCTT 
     2161 TCCTTGATGT TGTGATTGGG GGCCGTGCAG TGGAGACCCC TCCATTGTCT TCCGTCAATC 
     2221 TTCTGGAAGG ATTGAGCAGA ACTGTGGTTT ATATAACCTA CAGTCAGCTT ATTACTCTGG 
     2281 TGAATTTTAT GAAGAGTGTG ATGTCTGGAG ATCAACTGAG AGAAGATAGA ATGGCTCTTG 
     2341 ACAATTTATT GGCAAACCTA CCCCCGGCCA AGCCAGGAAA AAGTAGCAGT TTAGAAATGA 
     2401 CTCCCTACAA TACACCTCAG CTATCTCCAG CAACCACTCC AGCAAATAAA AAGAATCGAT 
     2461 TACCTATAGC AACTCGGAGC AGAAGCCGCA CCAATATGCT AATGGACCTA CATATGGACC 
     2521 ATGAAGGATC ATCTCAAGAA ACCATCCAGG AGGTGCAACC AGAAGAGGTG TTGGTCATTT 
     2581 CCTTAGGTAC AGGTCCCCAG CTTACTCCAG GGATGATGTC AGAAAATGAG GTCCTAAACA 
     2641 TGCAGCTTTC GGATGGAGGA CAAGGAGATG TCCCTGTTGA TGAAAACAAA CTCCATGGTA 
     2701 AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA ATATCTGAAG 
     2761 GTCCTTCAAA TCGCTCCAAT TCAGTGTCCT CCCTAGACCT AGAAGGAGAG TCTGTGTCAG 
     2821 AACTTGGAGC AGGACCTTCT GGCAGTAATG GAGTTGAAGC TCTACAGCTG TTAGAACATG 
     2881 AGCAAGCTAC AACACAGGAT AACCTTGATG ATAAGCTAAG GAAGTTTGAA ATTCGTGACA 
     2941 TGATGGGATT AACAGATGAT AGGGACATAT CAGAAACAGT GAGTGAGACC TGGAGTACAG 
     3001 ACGTCTTGGG AAGTGACTTT GACCCTAATA TTGATGAAGA TCGCTTGCAA GAAATTGCAG 
     3061 GTGCTGCAGC AGAGAACATG TTAGGCAGTT TGCTGTGCCT CCCAGGTTCA GGGTCAGTGC 
     3121 TTCTTGACCC CTGCACTGGT TCTACCATAT CAGAGACAAC AAGTGAAGCT TGGAGTGTAG 
     3181 AGGTATTGCC AAGTGACTCA GAGGCCCCAG ACCTAAAGCA GGAGGAGCGT CTGCAAGAAC 
     3241 TGGAGAGCTG TTCTGGACTG GGTAGCACAT CTGATGATAC GGATGTCAGG GAGGTCAGTT 
     3301 CCCGCCCCAG CACACCAGGC CTCAGTGTTG TGTCCGGCAT AAGTGCAACC TCTGAGGATA 
     3361 TTCCCAATAA GATTGAAGAC CTGAGATCTG AGTGCAGCTC TGATTTTGGG GGTAAAGATT 
     3421 CTGTCACTAG TCCAGACATG GATGAAATAA CTCACGGTGC CCACCAGCTG ACCTCTCCTC 
     3481 CTTCTCAGTC AGAGTCTCTG CTGGCCATGT TTGATCCACT GTCTTCACAT GAAGGGGCTT 
     3541 CTGCTGTGGT AAGGCCAAAG GTTCACTATG CTAGGCCATC GCATCCACCA CCAGATCCCC 
     3601 CAATCCTGGA AGGAGCTGTG GGAGGAAATG AGGCCAGGTT GCCAAACTTT GGTTCCCATG 
     3661 TTTTAACTCC AGCTGAAATG GAGGCATTCA AGCAAAGGCA TTCTTACCCT GAGAGACTAG 
     3721 TTCGAAGCAG GAGCTCTGAT ATAGTATCTT CTGTCCGGAG ACCCATGAGT GACCCCAGCT 
     3781 GGAACCGGCG TCCAGGAAAT GAAGAGCGAG AACTCCCTCC AGCTGCAGCC ATTGGTGCTA 
     3841 CTTCTTTGGT GGCTGCACCT CATTCATCAT CTTCATCCCC GAGTAAGGAC TCCTCAAGAG 
     3901 GAGAGACTGA AGAACGCAAA GATAGCGATG ATGAGAAATC AGACAGGAAC AGACCTTGGT 
     3961 GGAGAAAACG TTTTGTTTCA GCCATGCCTA AAGCTCCTAT ACCATTTAGA AAGAAAGAAA 
     4021 AACAAGAAAA AGACAAAGAT GATCTGGGGC CTGACAGATT CTCAACACTC ACAGATGATC 
     4081 CCAGCCCTAG ACTCAGTGCA CAAGCTCAGG TGGCTGAGGA TATTCTGGAC AAATACAGGA 
     4141 ATGCCATTAA ACGGACCAGC CCCAGTGATG GAGCAATGGC AAACTATGAA AGTACAGAGG 
     4201 TTATGGGTGA TGGTGAAAGT GCACATGATT CTCCCCGTGA CGAAGCACTG CAGAACATCT 
     4261 CGGCTGATGA TCTCCCAGAC TCTGCAAGCC AAGCAGCCCA CCCGCAGGAT TCAGCTTTCT 
     4321 CTTACAGAGA TGCAAAAAAG AAACTGAGGC TTGCTCTTTG CTCTGCGGAC TCTGTTGCCT 
     4381 TCCCAGTGCT GACCCATTCA ACAAGGAATG GTTTACCAGA CCACACAGAC CCAGAAGACA 
     4441 ATGAAATTGT ATGCTTCTTA AAAGTTCAAA TAGCTGAAGC AATTAATTTA CAAGATAAGA 
     4501 ATCTAATGGC TCAACTTCAA GAAACAATGC GCTGTGTGTG CCGTTTTGAT AATAGGACTT 
     4561 GTAGGAAACT GCTGGCTTCG ATTGCTGAGG ACTACAGAAA AAGAGCCCCA TATATTGCTT 
     4621 ATCTCACTCG TTGTCGACAA GGACTACAGA CCACACAGGC TCACCTGGAA AGGCTATTGC 
     4681 AAAGAGTTTT GCGGGACAAA GAAGTGGCCA ATCGATACTT TACCACTGTC TGTGTGAGAT 
     4741 TACTGCTTGA GAGCAAAGAA AAGAAGATCA GGGAATTCAT TCAAGACTTT CAGAAACTCA 
     4801 CCGCAGCTGA CGATAAAACT GCTCAGGTAG AAGATTTTCT GCAGTTTCTT TATGGTGCAA 
     4861 TGGCCCAGGA TGTCATATGG CAAAACGCGA GTGAAGAACA GCTTCAAGAT GCACAGCTGG 
     4921 CCATTGAGCG AAGCGTGATG AACCGGATTT TCAAGCTCGC CTTCTACCCT AATCAAGATG 
     4981 GGGACATACT TCGCGACCAG GTTCTTCATG AACATATCCA GAGATTGTCT AAAGTAGTGA 
     5041 CTGCAAATCA CAGAGCTCTT CAGATACCAG AGGTTTATCT TCGAGAAGCA CCATGGCCAT 
     5101 CTGCACAATC AGAAATCAGG ACAATAAGTG CTTATAAAAC CCCCCGGGAC AAAGTGCAGT 
     5161 GCATCCTGAG AATGTGCTCT ACGATTATGA ACCTCCTGAG CCTGGCCAAT GAGGACTCTG 
     5221 TCCCTGGAGC GGATGACTTT GTTCCTGTGT TGGTGTTTGT GTTGATAAAG GCAAATCCAC 
     5281 CCTGTTTGCT GTCTACTGTG CAGTATATCA GTAGCTTTTA TGCTAGCTGT CTGTCTGGAG 
     5341 AGGAGTCCTA TTGGTGGATG CAGTTCACAG CAGCAGTAGA ATTCATTAAA ACCATCGATG 
     5401 ACCGAAAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5461 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5521 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5581 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5641 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5701 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5761 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5821 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5881 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5941 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6001 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6061 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6121 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6181 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6241 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6301 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6361 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6421 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6481 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6541 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6601 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6661 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6721 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6781 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6841 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6901 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6961 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7021 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7081 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7141 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7201 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7261 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7321 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7381 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7441 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7501 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7561 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7621 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7681 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7741 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7801 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7861 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7921 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7981 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8041 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8101 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8161 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8221 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8281 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8341 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8401 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8461 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8521 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8581 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8641 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8701 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8761 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8821 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8881 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8941 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9001 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9061 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9121 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9181 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9241 CAAAAATTTA ACGCGAATTT T
//