LOCUS       RC240145                9198 bp    DNA     circular  SYN 
DEFINITION  GAPVD1 (myc-DDK-tagged) - Human GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant 3
ACCESSION   NM_001282681
VERSION     NM_001282681.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5355..5446 
                     /label="Myc-DDK"  
     misc_feature    5476..6061 
                     /label="PolyA signal"  
     misc_feature    5533..5552 
                     /label="Restriction Enzyme"  
     misc_feature    6337..7009 
                     /label="ColE1"  
     misc_feature    7439..8233 
                     /label="Kan/Neo"  
     misc_feature    8268..8570 
                     /label="SV40 ori"  
     misc_feature    8656..9133 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5337..5343 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5337 
                     /label="GAPVD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAAACTA GATATTCATA CTCTGGCTCA TCACCTCAAG CAGGAACGCT 
     1081 TATATGTAAA CTCTGAGAAA CAGCTCATTC AGAGGCTCAA TGCAGATGTA CTTAAGACAG 
     1141 CTGAAAAGTT GTATCGTACA GCATGGATTG CGAAGCAACA GAGAATCAAT TTGGATCGGC 
     1201 TTATCATAAC CAGTGCTGAA GCTTCCCCTG CTGAATGTTG CCAACATGCC AAAATTTTGG 
     1261 AAGATACACA ATTTGTTGAT GGGTATAAGC AATTGGGATT TCAGGAGACT GCTTATGGAG 
     1321 AATTCTTGAG TCGATTGAGG GAAAATCCTC GTCTTATTGC CTCCTCTTTG GTTGCTGGAG 
     1381 AGAAACTTAA TCAGGAGAAC ACACAAAGTG TTATTTACAC AGTTTTTACC TCCCTGTATG 
     1441 GCAATTGCAT CATGCAAGAA GATGAAAGCT ACCTCCTTCA GGTTTTGCGA TACTTGATTG 
     1501 AATTTGAACT TAAAGAAAGT GACAACCCTA GGCGACTTTT GAGGAGAGGA ACTTGTGCCT 
     1561 TCAGCATCTT ATTTAAACTT TTTTCTGAAG GACTGTTTTC TGCCAAACTT TTCCTCACAG 
     1621 CCACTTTACA TGAGCCAATT ATGCAACTGC TTGTTGAAGA TGAAGATCAC CTGGAAACAG 
     1681 ATCCAAACAA GCTAATTGAG AGGTTCTCTC CATCTCAGCA GGAAAAACTC TTTGGAGAGA 
     1741 AGGGCTCAGA TAGATTCAGG CAAAAAGTTC AAGAAATGGT GGAGTCCAAT GAAGCAAAGC 
     1801 TAGTGGCTTT GGTGAACAAA TTTATTGGTT ATCTCAAACA GAACACATAT TGTTTTCCAC 
     1861 ATAGTTTAAG GTGGATCGTG TCTCAGATGT ACAAAACCCT CTCCTGTGTA GATAGGCTGG 
     1921 AAGTTGGGGA GGTCAGGGCA ATGTGTACTG ATCTCCTGTT GGCCTGCTTC ATTTGTCCTG 
     1981 CAGTTGTCAA TCCAGAACAA TATGGAATAA TTTCCGATGC TCCTATTAAT GAAGTAGCAC 
     2041 GATTTAATCT GATGCAGGTA GGCCGCCTTT TGCAGCAGTT AGCAATGACT GGCTCTGAAG 
     2101 AGGGAGATCC CCGAACAAAG AGCAGCCTTG GAAAGTTTGA CAAAAGCTGT GTTGCCGCTT 
     2161 TCCTTGATGT TGTGATTGGG GGCCGTGCAG TGGAGACCCC TCCATTGTCT TCCGTCAATC 
     2221 TTCTGGAAGG ATTGAGCAGA ACTGTGGTTT ATATAACCTA CAGTCAGCTT ATTACTCTGG 
     2281 TGAATTTTAT GAAGAGTGTG ATGTCTGGAG ATCAACTGAG AGAAGATAGA ATGGCTCTTG 
     2341 ACAATTTATT GGCAAACCTA CCCCCGGCCA AGCCAGGAAA AAGTAGCAGT TTAGAAATGA 
     2401 CTCCCTACAA TACACCTCAG CTATCTCCAG CAACCACTCC AGCAAATAAA AAGAATCGAT 
     2461 TACCTATAGC AACTCGGAGC AGAAGCCGCA CCAATATGCT AATGGACCTA CATATGGACC 
     2521 ATGAAGGATC ATCTCAAGAA ACCATCCAGG AGGTGCAACC AGAAGAGGTG TTGGTCATTT 
     2581 CCTTAGGTAC AGGTCCCCAG CTTACTCCAG GGATGATGTC AGAAAATGAG GTCCTAAACA 
     2641 TGCAGCTTTC GGATGGAGGA CAAGGAGATG TCCCTGTTGA TGAAAACAAA CTCCATGGTC 
     2701 CTTCAAATCG CTCCAATTCA GTGTCCTCCC TAGACCTAGA AGGAGAGTCT GTGTCAGAAC 
     2761 TTGGAGCAGG ACCTTCTGGC AGTAATGGAG TTGAAGCTCT ACAGCTGTTA GAACATGAGC 
     2821 AAGCTACAAC ACAGGATAAC CTTGATGATA AGCTAAGGAA GTTTGAAATT CGTGACATGA 
     2881 TGGGATTAAC AGATGATAGG GACATATCAG AAACAGTGAG TGAGACCTGG AGTACAGACG 
     2941 TCTTGGGAAG TGACTTTGAC CCTAATATTG ATGAAGATCG CTTGCAAGAA ATTGCAGGTG 
     3001 CTGCAGCAGA GAACATGTTA GGCAGTTTGC TGTGCCTCCC AGGTTCAGGG TCAGTGCTTC 
     3061 TTGACCCCTG CACTGGTTCT ACCATATCAG AGACAACAAG TGAAGCTTGG AGTGTAGAGG 
     3121 TATTGCCAAG TGACTCAGAG GCCCCAGACC TAAAGCAGGA GGAGCGTCTG CAAGAACTGG 
     3181 AGAGCTGTTC TGGACTGGGT AGCACATCTG ATGATACGGA TGTCAGGGAG GTCAGTTCCC 
     3241 GCCCCAGCAC ACCAGGCCTC AGTGTTGTGT CCGGCATAAG TGCAACCTCT GAGGATATTC 
     3301 CCAATAAGAT TGAAGACCTG AGATCTGAGT GCAGCTCTGA TTTTGGGGGT AAAGATTCTG 
     3361 TCACTAGTCC AGACATGGAT GAAATAACTC ACGGTGCCCA CCAGCTGACC TCTCCTCCTT 
     3421 CTCAGTCAGA GTCTCTGCTG GCCATGTTTG ATCCACTGTC TTCACATGAA GGGGCTTCTG 
     3481 CTGTGGTAAG GCCAAAGGTT CACTATGCTA GGCCATCGCA TCCACCACCA GATCCCCCAA 
     3541 TCCTGGAAGG AGCTGTGGGA GGAAATGAGG CCAGGTTGCC AAACTTTGGT TCCCATGTTT 
     3601 TAACTCCAGC TGAAATGGAG GCATTCAAGC AAAGGCATTC TTACCCTGAG AGACTAGTTC 
     3661 GAAGCAGGAG CTCTGATATA GTATCTTCTG TCCGGAGACC CATGAGTGAC CCCAGCTGGA 
     3721 ACCGGCGTCC AGGAAATGAA GAGCGAGAAC TCCCTCCAGC TGCAGCCATT GGTGCTACTT 
     3781 CTTTGGTGGC TGCACCTCAT TCATCATCTT CATCCCCGAG TAAGGACTCC TCAAGAGGAG 
     3841 AGACTGAAGA ACGCAAAGAT AGCGATGATG AGAAATCAGA CAGGAACAGA CCTTGGTGGA 
     3901 GAAAACGTTT TGTTTCAGCC ATGCCTAAAG CTCCTATACC ATTTAGAAAG AAAGAAAAAC 
     3961 AAGAAAAAGA CAAAGATGAT CTGGGGCCTG ACAGATTCTC AACACTCACA GATGATCCCA 
     4021 GCCCTAGACT CAGTGCACAA GCTCAGGTGG CTGAGGATAT TCTGGACAAA TACAGGAATG 
     4081 CCATTAAACG GACCAGCCCC AGTGATGGAG CAATGGCAAA CTATGAAAGT ACAGAGGTTA 
     4141 TGGGTGATGG TGAAAGTGCA CATGATTCTC CCCGTGACGA AGCACTGCAG AACATCTCGG 
     4201 CTGATGATCT CCCAGACTCT GCAAGCCAAG CAGCCCACCC GCAGGATTCA GCTTTCTCTT 
     4261 ACAGAGATGC AAAAAAGAAA CTGAGGCTTG CTCTTTGCTC TGCGGACTCT GTTGCCTTCC 
     4321 CAGTGCTGAC CCATTCAACA AGGAATGGTT TACCAGACCA CACAGACCCA GAAGACAATG 
     4381 AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA GATAAGAATC 
     4441 TAATGGCTCA ACTTCAAGAA ACAATGCGCT GTGTGTGCCG TTTTGATAAT AGGACTTGTA 
     4501 GGAAACTGCT GGCTTCGATT GCTGAGGACT ACAGAAAAAG AGCCCCATAT ATTGCTTATC 
     4561 TCACTCGTTG TCGACAAGGA CTACAGACCA CACAGGCTCA CCTGGAAAGG CTATTGCAAA 
     4621 GAGTTTTGCG GGACAAAGAA GTGGCCAATC GATACTTTAC CACTGTCTGT GTGAGATTAC 
     4681 TGCTTGAGAG CAAAGAAAAG AAGATCAGGG AATTCATTCA AGACTTTCAG AAACTCACCG 
     4741 CAGCTGACGA TAAAACTGCT CAGGTAGAAG ATTTTCTGCA GTTTCTTTAT GGTGCAATGG 
     4801 CCCAGGATGT CATATGGCAA AACGCGAGTG AAGAACAGCT TCAAGATGCA CAGCTGGCCA 
     4861 TTGAGCGAAG CGTGATGAAC CGGATTTTCA AGCTCGCCTT CTACCCTAAT CAAGATGGGG 
     4921 ACATACTTCG CGACCAGGTT CTTCATGAAC ATATCCAGAG ATTGTCTAAA GTAGTGACTG 
     4981 CAAATCACAG AGCTCTTCAG ATACCAGAGG TTTATCTTCG AGAAGCACCA TGGCCATCTG 
     5041 CACAATCAGA AATCAGGACA ATAAGTGCTT ATAAAACCCC CCGGGACAAA GTGCAGTGCA 
     5101 TCCTGAGAAT GTGCTCTACG ATTATGAACC TCCTGAGCCT GGCCAATGAG GACTCTGTCC 
     5161 CTGGAGCGGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT GATAAAGGCA AATCCACCCT 
     5221 GTTTGCTGTC TACTGTGCAG TATATCAGTA GCTTTTATGC TAGCTGTCTG TCTGGAGAGG 
     5281 AGTCCTATTG GTGGATGCAG TTCACAGCAG CAGTAGAATT CATTAAAACC ATCGATGACC 
     5341 GAAAGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5401 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5461 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5521 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5581 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5641 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5701 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5761 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5821 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5881 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5941 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6001 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6061 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6121 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6181 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6241 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6301 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6361 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6421 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6481 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6541 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6601 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6661 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6721 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6781 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6841 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6901 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6961 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7021 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7081 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7141 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7201 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7261 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7321 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7381 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7441 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7501 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7561 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7621 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7681 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7741 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7801 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7861 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7921 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7981 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8041 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8101 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8161 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8221 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8281 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8341 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8401 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8461 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8521 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8581 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8641 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8701 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8761 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8821 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8881 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8941 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9001 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9061 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9121 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9181 AAATTTAACG CGAATTTT
//