LOCUS       RC240121                8946 bp    DNA     circular  SYN 
DEFINITION  USP47 (myc-DDK-tagged) - Human ubiquitin specific peptidase 47 (USP47), transcript variant 1
ACCESSION   NM_001282659
VERSION     NM_001282659.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5103..5194 
                     /label="Myc-DDK"  
     misc_feature    5224..5809 
                     /label="PolyA signal"  
     misc_feature    5281..5300 
                     /label="Restriction Enzyme"  
     misc_feature    6085..6757 
                     /label="ColE1"  
     misc_feature    7187..7981 
                     /label="Kan/Neo"  
     misc_feature    8016..8318 
                     /label="SV40 ori"  
     misc_feature    8404..8881 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5085..5091 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5085 
                     /label="USP47"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCCCGGC GAGGAGAACC AACTGGTCCC GAAAGAGATA GAAAATGCTG 
     1081 CTGAAGAACC TAGAGTCTTA TGTATTATAC AAGATACTAC TAATTCAAAG ACAGTGAATG 
     1141 AACGGATCAC TTTAAATTTA CCAGCATCTA CTCCAGTCAG AAAGCTCTTT GAAGATGTGG 
     1201 CCAACAAAGT AGGCTACATA AATGGAACCT TTGACTTGGT GTGGGGAAAT GGAATCAATA 
     1261 CTGCTGATAT GGCACCACTG GATCATACCA GTGACAAGTC ACTTCTCGAC GCTAATTTTG 
     1321 AGCCAGGAAA GAAGAACTTT CTGCATTTGA CAGATAAAGA TGGTGAACAA CCTCAAATAC 
     1381 TGCTGGAGGA TTCCAGTGCT GGGGAAGACA GTGTTCATGA CAGGTTTATA GGTCCGCTTC 
     1441 CAAGAGAAGG TTCTGGGGGT TCTACCAGTG ATTATGTCAG CCAAAGCTAC TCCTACTCAT 
     1501 CTATTTTGAA TAAATCAGAA ACTGGATATG TGGGACTAGT AAACCAAGCA ATGACTTGCT 
     1561 ATTTGAATAG CCTTTTGCAA ACACTTTTTA TGACTCCTGA ATTTAGGAAT GCATTATATA 
     1621 AGTGGGAATT TGAAGAATCT GAAGAAGATC CAGTGACAAG TATTCCATAC CAACTTCAAA 
     1681 GGCTTTTTGT TTTGTTACAA ACCAGCAAAA AGAGAGCAAT TGAAACCACA GATGTTACAA 
     1741 GGAGCTTTGG ATGGGATAGT AGTGAGGCTT GGCAGCAGCA TGATGTACAA GAACTATGCA 
     1801 GAGTCATGTT TGATGCTTTG GAACAGAAAT GGAAGCAAAC AGAACAGGCT GATCTTATAA 
     1861 ATGAGCTATA TCAAGGCAAG CTGAAGGACT ACGTGAGATG TCTGGAATGT GGTTATGAGG 
     1921 GCTGGCGAAT CGACACATAT CTTGATATTC CATTGGTCAT CCGACCTTAT GGGTCCAGCC 
     1981 AAGCATTTGC TAGTGTGGAA GAAGCATTGC ATGCATTTAT TCAGCCAGAG ATTCTGGATG 
     2041 GCCCAAATCA GTATTTTTGT GAACGTTGTA AGAAGAAGTG TGATGCACGG AAGGGCCTTC 
     2101 GGTTTTTGCA TTTTCCTTAT CTGCTGACCT TACAGCTGAA AAGATTCGAT TTTGATTATA 
     2161 CAACCATGCA TAGGATTAAA CTGAATGATC GAATGACATT TCCCGAGGAA CTAGATATGA 
     2221 GTACTTTTAT TGATGTTGAA GATGAGAAAT CTCCTCAGAC TGAAAGTTGC ACTGACAGTG 
     2281 GAGCAGAAAA TGAAGGTAGT TGTCACAGTG ATCAGATGAG CAACGATTTC TCCAATGATG 
     2341 ATGGTGTTGA TGAAGGAATC TGTCTTGAAA CCAATAGTGG AACTGAAAAG ATCTCAAAAT 
     2401 CTGGACTTGA AAAGAATTCC TTGATCTATG AACTTTTCTC TGTTATGGTT CATTCTGGGA 
     2461 GCGCTGCTGG TGGTCATTAT TATGCATGTA TAAAGTCATT CAGTGATGAG CAGTGGTACA 
     2521 GCTTCAATGA TCAACATGTC AGCAGGATAA CACAAGAGGA CATTAAGAAA ACACATGGTG 
     2581 GATCTTCAGG AAGCAGAGGA TATTATTCTA GTGCTTTCGC AAGTTCCACA AATGCATATA 
     2641 TGCTGATCTA TAGACTGAAG GATCCAGCCA GAAATGCAAA ATTTCTAGAA GTGGATGAAT 
     2701 ACCCAGAACA TATTAAAAAC TTGGTGCAGA AAGAGAGAGA GTTGGAAGAA CAAGAAAAGA 
     2761 GACAACGAGA AATTGAGCGC AATACATGCA AGATAAAATT ATTCTGTTTG CATCCTACAA 
     2821 AACAAGTAAT GATGGAAAAT AAATTGGAGG TTCATAAGGA TAAGACATTA AAGGAAGCAG 
     2881 TAGAAATGGC TTATAAGATG ATGGATTTAG AAGAGGTAAT ACCCCTGGAT TGCTGTCGCC 
     2941 TTGTTAAATA TGATGAGTTT CATGATTATC TAGAACGGTC ATATGAAGGA GAAGAAGATA 
     3001 CACCAATGGG GCTTCTACTA GGTGGCGTCA AGTCAACATA TATGTTTGAT CTGCTGTTGG 
     3061 AGACGAGAAA GCCTGATCAG GTTTTCCAAT CTTATAAACC TGGAGAAGTG ATGGTGAAAG 
     3121 TTCATGTTGT TGATCTAAAG GCAGAATCTG TAGCTGCTCC TATAACTGTT CGTGCTTACT 
     3181 TAAATCAGAC AGTTACAGAA TTCAAACAAC TGATTTCAAA GGCCATCCAT TTACCTGCTG 
     3241 AAACAATGAG AATAGTGCTG GAACGCTGCT ACAATGATTT GCGTCTTCTC AGTGTCTCCA 
     3301 GTAAAACCCT GAAAGCTGAA GGATTTTTTA GAAGTAACAA GGTGTTTGTT GAAAGCTCCG 
     3361 AGACTTTGGA TTACCAGATG GCCTTTGCAG ACTCTCATTT ATGGAAACTC CTGGATCGGC 
     3421 ATGCAAATAC AATCAGATTA TTTGTTTTGC TACCTGAACA ATCCCCAGTA TCTTATTCCA 
     3481 AAAGGACAGC ATACCAGAAA GCTGGAGGCG ATTCTGGTAA TGTGGATGAT GACTGTGAAA 
     3541 GAGTCAAAGG ACCTGTAGGA AGCCTAAAGT CTGTGGAAGC TATTCTAGAA GAAAGCACTG 
     3601 AAAAACTCAA AAGCTTGTCA CTGCAGCAAC AGCAGGATGG AGATAATGGG GACAGCAGCA 
     3661 AAAGTACTGA GACAAGTGAC TTTGAAAACA TCGAATCACC TCTCAATGAG AGGGACTCTT 
     3721 CAGCATCAGT GGATAATAGA GAACTTGAAC AGCATATTCA GACTTCTGAT CCAGAAAATT 
     3781 TTCAGTCTGA AGAACGATCA GACTCAGATG TGAATAATGA CAGGAGTACA AGTTCAGTGG 
     3841 ACAGTGATAT TCTTAGCTCC AGTCATAGCA GTGATACTTT GTGCAATGCA GACAATGCTC 
     3901 AGATCCCTTT GGCTAATGGA CTTGACTCTC ACAGTATCAC AAGTAGTAGA AGAACGAAAG 
     3961 CAAATGAAGG GAAAAAAGAA ACATGGGATA CAGCAGAAGA AGACTCTGGA ACTGATAGTG 
     4021 AATATGATGA GAGTGGCAAG AGTAGGGGAG AAATGCAGTA CATGTATTTC AAAGCTGAAC 
     4081 CTTATGCTGC AGATGAAGGT TCTGGGGAAG GACATAAATG GTTGATGGTG CATGTTGATA 
     4141 AAAGAATTAC TCTGGCAGCT TTCAAACAAC ATTTAGAGCC CTTTGTTGGA GTTTTGTCCT 
     4201 CTCACTTCAA GGTCTTTCGA GTGTATGCCA GCAATCAAGA GTTTGAGAGC GTCCGGCTGA 
     4261 ATGAGACACT TTCATCATTT TCTGATGACA ATAAGATTAC AATTAGACTG GGGAGAGCAC 
     4321 TTAAAAAAGG AGAATACAGA GTTAAAGTAT ACCAGCTTTT GGTCAATGAA CAAGAGCCAT 
     4381 GCAAGTTTCT GCTAGATGCT GTGTTTGCTA AAGGAATGAC TGTACGGCAA TCAAAAGAGG 
     4441 AATTAATTCC TCAGCTCAGG GAGCAATGTG GTTTAGAGCT CAGTATTGAC AGGTTTCGTC 
     4501 TAAGGAAAAA AACATGGAAG AATCCTGGCA CTGTCTTTTT GGATTATCAT ATTTATGAAG 
     4561 AAGATATTAA TATTTCCAGC AACTGGGAGG TTTTCCTTGA AGTTCTTGAT GGGGTAGAGA 
     4621 AGATGAAGTC CATGTCACAG CTTGCAGTTT TGTCAAGACG GTGGAAGCCT TCAGAGATGA 
     4681 AGTTGGATCC CTTCCAGGAG GTTGTATTGG AAAGCAGTAG TGTGGACGAA TTGCGAGAGA 
     4741 AGCTTAGTGA AATCAGTGGG ATTCCTTTGG ATGATATTGA ATTTGCTAAG GGTAGAGGAA 
     4801 CATTTCCCTG TGATATTTCT GTCCTTGATA TTCATCAGGA TTTAGACTGG AATCCTAAAG 
     4861 TTTCTACCCT GAATGTCTGG CCTCTTTATA TCTGTGATGA TGGTGCGGTC ATATTTTATA 
     4921 GGGATAAAAC AGAAGAATTA ATGGAATTGA CAGATGAGCA AAGAAATGAA CTGATGAAAA 
     4981 AAGAAAGCAG TCGACTCCAG AAGACTGGAC ATCGTGTAAC ATACTCACCT CGTAAAGAGA 
     5041 AAGCACTAAA AATATATCTG GATGGAGCAC CAAATAAAGA TCTGACTCAA GACACGCGTA 
     5101 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5161 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5221 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5281 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5341 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5401 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5461 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5521 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5581 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5641 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5701 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5761 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5821 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5881 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5941 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6001 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6061 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6121 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6181 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6241 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6301 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6361 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6421 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6481 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6541 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6601 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6661 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6721 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6781 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6841 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6901 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6961 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7021 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7081 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7141 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7201 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7261 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7321 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7381 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7441 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7501 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7561 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7621 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7681 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7741 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7801 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7861 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7921 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7981 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8041 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8101 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8161 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8221 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8281 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8341 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8401 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8461 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8521 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8581 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8641 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8701 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8761 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8821 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8881 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8941 AATTTT
//