LOCUS       RC240113                8892 bp    DNA     circular  SYN 
DEFINITION  SCAF8 (myc-DDK-tagged) - Human SR-related CTD-associated factor 8 (SCAF8), transcript variant 2
ACCESSION   NM_001286189
VERSION     NM_001286189.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5049..5140 
                     /label="Myc-DDK"  
     misc_feature    5170..5755 
                     /label="PolyA signal"  
     misc_feature    5227..5246 
                     /label="Restriction Enzyme"  
     misc_feature    6031..6703 
                     /label="ColE1"  
     misc_feature    7133..7927 
                     /label="Kan/Neo"  
     misc_feature    7962..8264 
                     /label="SV40 ori"  
     misc_feature    8350..8827 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5031..5037 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5031 
                     /label="SCAF8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCAGCCG CTGCTGCCAG CGCTTCCTCC TCTGTCTTCG CCGAGCGGGG 
     1081 CTGGTTCCTG CGGCCCGAGC GGCGGGGAGT GCAGTGGCCG CCGCCTCTTC CGCCGCCGGG 
     1141 CTCGGGGCCT CCGCAGCGAC AACATGGAGG CCGTGAAGAC CTTCAATAGC GAGGATTGTG 
     1201 CCTCTACTCC TGCTTGTACA GACTGCTTTT CCAGCAGCAT GGACACAAGA AGCATATTGT 
     1261 ATTCCCTGAA TGACTATAAA CCACCCATTT CGAAAGCGAA AATGACCCAA ATTACTAAGG 
     1321 CAGCCATCAA AGCTATTAAG TTCTATAAAC ATGTGGTACA GAGTGTTGAG AAGTTTATTC 
     1381 AGAAATGTAA ACCAGAATAC AAAGTACCTG GACTTTATGT TATTGACTCC ATTGTGCGAC 
     1441 AATCCCGACA TCAGTTTGGT CAAGAAAAGG ATGTGTTTGC ACCCAGATTT AGTAATAACA 
     1501 TCATTAGCAC TTTCCAGAAT TTATATCGTT GCCCTGGGGA TGACAAGAGT AAAATAGTGA 
     1561 GAGTACTAAA CTTATGGCAG AAGAATAATG TATTTAAGAG TGAGATTATT CAGCCCCTTT 
     1621 TGGATATGGC AGCCGGGATT CCGCCTCCAG TTGTCACACC TGTTTTGGCC AGCACTACCA 
     1681 CTGCTATGAG CAATACTCCA GGAACTCCTG TGACACCTGT TACTCCGGCC AATGTGGTCC 
     1741 AAGGCTTACC TGATCCGTGG GTATCTCAGA TAACAAATAC AGATACACTT GCGGCTGTAG 
     1801 CTCAGATCTT GCAAAGTCCT CAAGGCCAGC AGCTTCAACA ATTAATACAA ACCTTACAGA 
     1861 TACAACAACA GAAGCCCCAG CCTTCCATTC TGCAGGCCCT AGATGCTGGT CTTGTTGTTC 
     1921 AGTTGCAAGC TCTTACGGCA CAACTTACAG CTGCAGCTGC AGCTGCCAAC ACTCTTACTC 
     1981 CCTTAGAACA GGGAGTCTCC TTTAACAAGA AGTTGATGGA TAGGTTTGAT TTTGGGGAAG 
     2041 ACTCTGAGCA TAGTGAAGAA CCCAAAAAGG AAATTCCAGC TTCACAACTT TCTCACGTTT 
     2101 CAGAATCTGT GAACAATTCC ATTTTTCATC AGATAGCAGA ACAACTACAA CAGCAAAACC 
     2161 TAGAACATCT CAGACAGCAG CTCTTGGAGC AGCAACAGCC TCAAAAGGCC ACTCCTCAGG 
     2221 ATAGTCAGGA AGGAACCTTT GGGTCAGAGC ATTCAGCGTC ACCATCACAA GGGAGTAGTC 
     2281 AGCAGCATTT TCTTGAACCT GAAGTCAATT TGGATGATTC CATAGATATT CAGCAACAGG 
     2341 ATATGGATAT AGATGAAGGG CAAGATGGAG TGGAAGAGGA GGTCTTTGAA CAAGAAGCTA 
     2401 AGAAAGTGGC GGTTCGCTCA AGATCAAGAA CACATTCACG ATCTCGTTCA AGATCACCAA 
     2461 GAAAACGAAG GTCTAGGTCA CGGTCTGGCT CTAGAAAGCG TAAACACAGA AAGCGATCAC 
     2521 GCTCCCGCTC AAGAGAAAGA AAGAGGAAAT CATCACGGTC GTATTCAAGT GAAAGGAGAG 
     2581 CCAGAGAAAG GGAGAAAGAA CGACAGAAAA AGGGATTACC TCCAATTAGA TCTAAAACAC 
     2641 TAAGTGTATG TAGTACTACT CTCTGGGTTG GGCAAGTGGA CAAGAAGGCA ACACAGCAAG 
     2701 ACTTAACCAA CCTGTTTGAA GAGTTTGGAC AGATTGAATC CATTAATATG ATTCCTCCCC 
     2761 GGGGCTGTGC TTATGTCTGC ATGGTTCATC GACAAGATGC ATTTCGAGCT CTTCAGAAAC 
     2821 TCAGTTCTGG ATCATATAAA ATTGGGTCCA AGGTCATTAA GATCGCTTGG GCTTTAAACA 
     2881 AAGGTGTAAA AACAGAATAC AAACAATTCT GGGATGTGGA TCTTGGAGTT ACATATATAC 
     2941 CATGGGAAAA AGTTAAAGTG GATGACTTGG AAGGTTTTGC AGAAGGAGGC ATGATTGATC 
     3001 AGGAGACTGT AAATACTGAG TGGGAAACTG TGAAAAGCTC AGAACCTGTT AAAGAGACGG 
     3061 TCCAGACAAC TCAGAGCCCA ACTCCAGTTG AAAAGGAGAC AGTGGTCACA ACCCAGGCAG 
     3121 AGGTTTTCCC TCCTCCTGTT GCTATGTTGC AGATTCCAGT GGCGCCAGCC GTGCCTACAG 
     3181 TTAGTTTAGT CCCACCAGCA TTTCCTGTGT CGATGCCGGT TCCTCCTCCT GGATTCAGTC 
     3241 CAATCCCTCC ACCTCCTTTT TTAAGAGCAA GTTTTAACCC TTCACAACCA CCACCTGGTT 
     3301 TCATGCCGCC TCCAGTTCCC CCACCTGTTG TGCCACCCCC TACGATTCCA CCAGTAGTAC 
     3361 CAACATCTTT AGTGCAGCCG TCATTATCCA TGACACCGGA AACTGTGAAA GATGTTGGAT 
     3421 TTGGTAGCCT TGTTATACCA GGCGGTTCTG TTGCCAGCAA TCTTGCTACT TCCGCTCTGC 
     3481 CAGCTGGAAA TGTTTTTAAT GCTCCAACTA AACAGGCAGA GCCTGAAGAA AAAGTACCTC 
     3541 ATCTTATAGA CCACCAGATT TCTTCTGGTG AAAACACCAG ATCAGTGATT CCAAATGATA 
     3601 TTTCAAGTAA TGCTGCAATT TTAGGAGGAC AGCCGCCAAA TGTGACAAGC AATTCTGGAA 
     3661 TTCTGGGAGT CCAAAGACCA AATGTATCAA GTAATTCTGA AATTCTTGGG GTCCGGCCAT 
     3721 CTAATGTTTC CAGTAGTTCT GGGATTATTG CAGCCCAACC ACCAAATATT CTAAATAACT 
     3781 CTGGAATATT GGGAATACAG CCACCCAGTG TGTCAAATAG TTCTGGACTT TTGGGAGTGC 
     3841 TACCCCCAAA TATACCTAAC AATTCTGGAC TTGTAGGAGT ACAGCCACCA AATGTTCCAA 
     3901 ATACTCCTGG ACTTCTGGGA ACACAGCCAC CAGCTGGACC TCAAAACTTA CCCCCTTTAA 
     3961 GTATCCCTAA TCAAAGGATG CCCACAATGC CAATGTTAGA CATTCGTCCG GGACTAATAC 
     4021 CACAGGCACC TGGGCCAAGA TTCCCTTTAA TACAGCCTGG AATTCCACCC CAACGGGGAA 
     4081 TCCCACCCCC ATCGGTACTT GATTCAGCTC TTCATCCACC ACCCCGTGGA CCTTTTCCTC 
     4141 CAGGAGATAT TTTTAGTCAA CCAGAAAGAC CTTTTTTAGC TCCTGGAAGA CAAAGCGTAG 
     4201 ACAATGTTAC TAACCCAGAA AAAAGGATAC CACTTGGGAA TGATAACATT CAACAGGAAG 
     4261 GAGATAGAGA TTACCGGTTT CCTCCTATAG AAACCAGGGA AAGCATTAGT AGACCTCCCC 
     4321 CTGTGGATGT TAGAGATGTG GTTGGGCGGC CTATAGATCC AAGAGAAGGT CCTGGACGGC 
     4381 CTCCACTAGA TGGTAGGGAT CATTTTGGAA GACCTCCTGT AGATATAAGA GAGAATCTTG 
     4441 TGAGGCCAGG TATAGATCAT CTTGGTCGAA GAGACCACTT TGGCTTTAAT CCAGAGAAGC 
     4501 CCTGGGGGCA TAGAGGAGAT TTTGATGAGA GAGAGCATCG GGTTCTACCG GTCTATGGTG 
     4561 GTCCAAAAGG CTTACATGAA GAAAGAGGTA GATTTCGGTC TGGAAACTAT CGATTTGATC 
     4621 CTAGAAGTGG TCCTTGGAAC CGAGGATTTG GACAAGAAGT TCACAGAGAT TTTGATGACC 
     4681 GCAGAAGACC CTGGGAGAGG CAAAGGGATA GGGATGACAG AGATTTTGAT TTCTGCAGAG 
     4741 AAATGAATGG AAATCGTCTT GGACGAGACA GAATTCAAAA CACTTGGGTT CCCCCTCCTC 
     4801 ATGCTCGGGT TTTTGATTAT TTTGAAGGGG CCACTTCTCA ACGAAAAGGT GATAATGTGC 
     4861 CTCAGGTTAA TGGTGAAAAT ACAGAGAGAC ATGCTCAGCC ACCACCTATA CCAGTACAGA 
     4921 ATGATCCTGA ACTTTATGAA AAACTGACAT CTTCAAATGA AATAAACAAG GAGAAGAGTG 
     4981 ACACAGTTGC TGATATAGAA AGTGAACCAG TGGTAGAAAG CACAGAAACT GAGGGGACAA 
     5041 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5101 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5161 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5221 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5281 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5341 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5401 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5461 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5521 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5581 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5641 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5701 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5761 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5821 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6721 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6781 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6841 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6901 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6961 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7021 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7081 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7141 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7201 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7261 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7321 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7381 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7441 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7501 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7561 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7621 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7681 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7741 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7801 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7861 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7921 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7981 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8041 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8101 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8881 AACGCGAATT TT
//