LOCUS       RC240079                8664 bp    DNA     circular  SYN 
DEFINITION  SIK3 (myc-DDK-tagged) - Human SIK family kinase 3 (SIK3), transcript variant 3
ACCESSION   NM_001281749
VERSION     NM_001281749.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4821..4912 
                     /label="Myc-DDK"  
     misc_feature    4942..5527 
                     /label="PolyA signal"  
     misc_feature    4999..5018 
                     /label="Restriction Enzyme"  
     misc_feature    5803..6475 
                     /label="ColE1"  
     misc_feature    6905..7699 
                     /label="Kan/Neo"  
     misc_feature    7734..8036 
                     /label="SV40 ori"  
     misc_feature    8122..8599 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4803..4809 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4803 
                     /label="SIK3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCGGCGAGCG GAGCTGGCGG GGCTGCCGGG GCCGGGACTG 
     1081 GGGGAGCCGG GCCCGCGGGC CGCCTGCTGC CTCCGCCCGC GCCGGGGTCC CCAGCCGCCC 
     1141 CCGCTGCCGT GTCCCCTGCG GCCGGCCAGC CGCGTCCCCC AGCCCCGGCC TCCCGCGGAC 
     1201 CCATGCCCGC CCGTATCGGC TACTACGAGA TCGACCGCAC CATCGGCAAG GGCAACTTCG 
     1261 CGGTGGTCAA GCGGGCCACG CACCTCGTCA CCAAGGCCAA GGTTGCTATC AAGATCATAG 
     1321 ATAAGACCCA GCTGGATGAA GAAAACTTGA AGAAGATTTT CCGGGAAGTT CAAATTATGA 
     1381 AGATGCTTTG CCACCCCCAT ATCATCAGGC TCTACCAGGT TATGGAGACA GAACGGATGA 
     1441 TTTATCTGGT GACAGAATAT GCTAGTGGAG GGGAAATATT TGACCACCTG GTGGCCCATG 
     1501 GTAGAATGGC AGAAAAGGAG GCACGTCGGA AGTTCAAACA GATCGTCACA GCTGTCTATT 
     1561 TTTGTCACTG TCGGAACATT GTTCATCGTG ATTTAAAAGC TGAAAATTTA CTTCTGGATG 
     1621 CCAATCTGAA TATCAAAATA GCAGATTTTG GTTTCAGTAA CCTCTTCACT CCTGGGCAGC 
     1681 TGCTGAAGAC CTGGTGTGGC AGCCCTCCCT ATGCTGCACC TGAACTCTTT GAAGGAAAAG 
     1741 AATATGATGG GCCCAAAGTG GACATCTGGA GCCTTGGAGT TGTCCTCTAC GTGCTTGTGT 
     1801 GCGGTGCCCT GCCATTTGAT GGAAGCACAC TGCAGAATCT GCGGGCCCGC GTGCTGAGTG 
     1861 GAAAGTTCCG CATCCCATTT TTTATGTCCA CAGAATGTGA GCATTTGATC CGCCATATGT 
     1921 TGGTGTTAGA TCCCAATAAG CGCCTCTCCA TGGAGCAGAT CTGCAAGCAC AAGTGGATGA 
     1981 AGCTAGGGGA CGCCGATCCC AACTTTGACA GGTTAATAGC TGAATGCCAA CAACTAAAGG 
     2041 AAGAAAGACA GGTGGACCCC CTGAATGAGG ATGTCCTCTT GGCCATGGAG GACATGGGAC 
     2101 TGGACAAAGA ACAGACACTG CAGGCGGAGC AGGCAGGTAC TGCTATGAAC ATCAGCGTTC 
     2161 CCCAGGTGCA GCTGATCAAC CCAGAGAACC AAATTGTGGA GCCGGATGGG ACACTGAATT 
     2221 TGGACAGTGA TGAGGGTGAA GAGCCTTCCC CTGAAGCATT GGTGCGCTAT TTGTCAATGA 
     2281 GGAGGCACAC AGTGGGTGTG GCTGACCCAC GCACGGAAGT TATGGAAGAT CTGCAGAAGC 
     2341 TCCTACCTGG CTTTCCTGGA GTCAACCCCC AGGCTCCATT CCTGCAGGTG GCCCCTAATG 
     2401 TGAACTTCAT GCACAACCTG TTGCCTATGC AAAACTTGCA ACCAACCGGG CAACTTGAGT 
     2461 ACAAGGAGCA GTCTCTCCTA CAGCCGCCCA CGCTACAGCT GTTGAATGGA ATGGGCCCCC 
     2521 TTGGCCGGAG GGCATCAGAT GGAGGAGCCA ACATCCAACT GCATGCCCAG CAGCTGCTGA 
     2581 AGCGCCCACG GGGACCCTCT CCGCTTGTCA CCATGACACC AGCAGTGCCA GCAGTTACCC 
     2641 CTGTGGACGA GGAGAGCTCA GACGGGGAGC CAGACCAGGA AGCTGTGCAG AGGTACTTGG 
     2701 CAAATAGGTC CAAAAGACAT ACACTGGCCA TGACCAACCC TACAGCTGAG ATCCCACCGG 
     2761 ACCTACAACG GCAGCTAGGA CAGCAGCCTT TCCGTTCCCG GGTCTGGCCT CCTCACCTGG 
     2821 TACCTGATCA GCATCGCTCT ACCTACAAGG ACTCCAACAC TCTGCACCTC CCTACGGAGC 
     2881 GTTTCTCCCC TGTGCGCCGG TTCTCAGATG GGGCTGCGAG CATCCAGGCC TTCAAAGCTC 
     2941 ACCTGGAAAA AATGGGCAAC AACAGCAGCA TCAAACAGCT GCAGCAGGAG TGTGAGCAGC 
     3001 TGCAGAAGAT GTACGGGGGG CAGATTGATG AAAGAACCCT GGAGAAGACC CAGCAGCAGC 
     3061 ATATGTTATA CCAGCAGGAG CAGCACCATC AAATTCTCCA GCAACAAATT CAAGACTCTA 
     3121 TCTGTCCTCC TCAGCCATCT CCACCTCTTC AGGCTGCATG TGAAAATCAG CCAGCCCTCC 
     3181 TTACCCATCA GCTCCAGAGG TTAAGGATTC AGCCTTCAAG CCCACCCCCC AACCACCCCA 
     3241 ACAACCATCT CTTCAGGCAG CCCAGTAATA GTCCTCCCCC CATGAGCAGT GCCATGATCC 
     3301 AGCCTCACGG GGCTGCATCT TCTTCCCAGT TTCAAGGCTT ACCTTCCCGC AGTGCAATCT 
     3361 TTCAGCAGCA ACCTGAGAAC TGTTCCTCTC CTCCCAACGT GGCACTAACC TGCTTGGGTA 
     3421 TGCAGCAGCC TGCTCAGTCA CAGCAGGTCA CCATCCAAGT CCAAGAGCCT GTTGACATGC 
     3481 TCAGCAACAT GCCAGGCACA GCTGCAGGCT CCAGTGGGCG CGGCATCTCC ATCAGCCCCA 
     3541 GTGCTGGTCA GATGCAGATG CAGCACCGTA CCAACCTGAT GGCCACCCTC AGCTATGGGC 
     3601 ACCGTCCCTT GTCCAAGCAG CTGAGTGCTG ACAGTGCAGA GGCTCACAGT GCACATCAGC 
     3661 AGCCGCCACA CTATACCACG TCGGCACTAC AGCAGGCCCT GCTGTCTCCC ACGCCGCCAG 
     3721 ACTATACAAG ACACCAGCAG GTACCCCACA TCCTTCAAGG ACTGCTTTCT CCCCGGCATT 
     3781 CGCTCACCGG CCACTCGGAC ATCCGGCTGC CCCCAACAGA GTTTGCACAG CTCATTAAAA 
     3841 GGCAGCAGCA ACAACGGCAG CAGCAGCAGC AACAGCAGCA ACAGCAAGAA TACCAGGAAC 
     3901 TGTTCAGGCA CATGAACCAA GGGGATGCGG GGAGTCTGGC TCCCAGCCTT GGGGGACAGA 
     3961 GCATGACAGA GCGCCAGGCT TTATCTTATC AAAATGCTGA CTCTTATCAC CATCACACCA 
     4021 GCCCCCAGCA TCTGCTACAA ATCAGGGCAC AAGAATGTGT CTCACAGGCT TCCTCACCCA 
     4081 CCCCGCCCCA CGGGTATGCT CACCAGCCGG CACTGATGCA TTCAGAGAGC ATGGAGGAGG 
     4141 ACTGCTCGTG TGAGGGGGCC AAGGATGGCT TCCAAGACAG TAAGAGTTCA AGTACATTGA 
     4201 CCAAAGGTTG CCATGACAGC CCTCTGCTCT TGAGTACCGG TGGACCTGGG GACCCTGAAT 
     4261 CTTTGCTAGG AACTGTGAGT CATGCCCAAG AATTGGGGAT ACATCCCTAT GGTCATCAGC 
     4321 CAACTGCTGC ATTCAGTAAA AATAAGGTGC CCAGCAGAGA GCCTGTCATA GGGAACTGCA 
     4381 TGGATAGAAG TTCTCCAGGA CAAGCAGTGG AGCTGCCGGA TCACAATGGG CTCGGGTACC 
     4441 CAGCACGCCC CTCCGTCCAT GAGCACCACA GGCCCCGGGC CCTCCAGAGA CACCACACGA 
     4501 TCCAGAACAG CGACGATGCT TATGTACAGC TGGATAACTT GCCAGGAATG AGTCTCGTGG 
     4561 CTGGGAAAGC ACTTAGCTCT GCCCGGATGT CGGATGCAGT TCTCAGTCAG TCTTCGCTCA 
     4621 TGGGCAGCCA GCAGTTTCAG GATGGGGAAA ATGAGGAATG TGGGGCAAGC CTGGGAGGTC 
     4681 ATGAGCACCC AGACCTGAGT GATGGCAGCC AGCATTTAAA CTCCTCTTGC TATCCATCTA 
     4741 CGTGTATTAC AGACATTCTG CTCAGCTACA AGCACCCCGA AGTCTCCTTC AGCATGGAGC 
     4801 AGGCAGGCGT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4861 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4921 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4981 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5041 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5101 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5161 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5221 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5281 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5341 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5401 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5461 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5521 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5581 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5641 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5701 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5761 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5821 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5881 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5941 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6001 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6061 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6121 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6181 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6241 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6301 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6361 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6421 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6481 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6541 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6601 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6661 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6721 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6781 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6841 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6901 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6961 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7021 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7081 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7141 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7201 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7261 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7321 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7381 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7441 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7501 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7561 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7621 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7681 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7741 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7801 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7861 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7921 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7981 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8041 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8101 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8161 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8221 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8281 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8341 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8401 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8461 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8521 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8581 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8641 TAACAAAAAT TTAACGCGAA TTTT
//