LOCUS       RC240025                8412 bp    DNA     circular  SYN 
DEFINITION  LPHN2 (myc-DDK-tagged) - Human latrophilin 2 (LPHN2), transcript variant 3
ACCESSION   NM_001297705
VERSION     NM_001297705.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4569..4660 
                     /label="Myc-DDK"  
     misc_feature    4690..5275 
                     /label="PolyA signal"  
     misc_feature    4747..4766 
                     /label="Restriction Enzyme"  
     misc_feature    5551..6223 
                     /label="ColE1"  
     misc_feature    6653..7447 
                     /label="Kan/Neo"  
     misc_feature    7482..7784 
                     /label="SV40 ori"  
     misc_feature    7870..8347 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4551..4557 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4551 
                     /label="ADGRL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGTCTTCT GGTTGCAGAA TGCGAAGTCT GTGGTTTATC ATTGTAATCA 
     1081 GCTTCTTACC AAATACAGAA GGTTTCAGCA GAGCAGCTTT ACCATTTGGG CTGGTGAGGC 
     1141 GAGAATTATC CTGTGAAGGT TATTCTATAG ATCTGCGATG CCCGGGCAGT GATGTCATCA 
     1201 TGATTGAGAG CGCTAACTAT GGTCGGACGG ATGACAAGAT TTGTGATGCT GACCCATTTC 
     1261 AGATGGAGAA TACAGACTGC TACCTCCCCG ATGCCTTCAA AATTATGACT CAAAGGTGCA 
     1321 ACAATCGAAC ACAGTGTATA GTAGTTACTG GGTCAGATGT GTTTCCTGAT CCATGTCCTG 
     1381 GAACATACAA ATACCTTGAA GTCCAATATG AATGTGTCCC TTACATTTTT GTGTGTCCTG 
     1441 GGACCTTGAA AGCAATTGTG GACTCACCAT GTATATATGA AGCTGAACAA AAGGCGGGTG 
     1501 CTTGGTGCAA GGACCCTCTT CAGGCTGCAG ATAAAATTTA TTTCATGCCC TGGACTCCCT 
     1561 ATCGTACCGA TACTTTAATA GAATATGCTT CTTTAGAAGA TTTCCAAAAT AGTCGCCAAA 
     1621 CAACAACATA TAAACTTCCA AATCGAGTAG ATGGTACTGG ATTTGTGGTG TATGATGGTG 
     1681 CTGTCTTCTT TAACAAAGAA AGAACGAGGA ATATTGTGAA ATTTGACTTG AGGACTAGAA 
     1741 TTAAGAGTGG CGAGGCCATA ATTAACTATG CCAACTACCA TGATACCTCA CCATACAGAT 
     1801 GGGGAGGAAA GACTGATATC GACCTAGCAG TTGATGAAAA TGGTTTATGG GTCATTTACG 
     1861 CCACTGAACA GAACAATGGA ATGATAGTTA TTAGCCAGCT GAATCCATAC ACTCTTCGAT 
     1921 TTGAAGCAAC GTGGGAGACT GTATACGACA AACGTGCCGC ATCAAATGCT TTTATGATAT 
     1981 GCGGAGTCCT CTATGTGGTT AGGTCAGTTT ATCAAGACAA TGAAAGTGAA ACAGGCAAGA 
     2041 ACTCAATTGA TTACATTTAT AATACCCGAT TAAACCGAGG AGAATATGTA GATGTTCCCT 
     2101 TCCCCAACCA GTATCAGTAT ATTGCTGCAG TGGATTACAA TCCAAGAGAT AACCAACTTT 
     2161 ACGTGTGGAA CAATAACTTC ATTTTACGAT ATTCTCTGGA GTTTGGTCCA CCTGATCCTG 
     2221 CCCAAGTGCC TACCACAGCT GTGACAATAA CTTCTTCAGC TGAGCTGTTC AAAACCATAA 
     2281 TATCAACCAC AAGCACTACT TCACAGAAAG GCCCCATGAG CACAACTGTA GCTGGATCAC 
     2341 AGGAAGGAAG CAAAGGGACA AAACCACCTC CAGCAGTTTC TACAACCAAA ATTCCACCTA 
     2401 TAACAAATAT TTTTCCCCTG CCAGAGAGAT TCTGTGAAGC ATTAGACTCC AAGGGGATAA 
     2461 AGTGGCCTCA GACACAAAGG GGAATGATGG TTGAACGACC ATGCCCTAAG GGAACAAGAG 
     2521 GAACTGCCTC ATATCTCTGC ATGATTTCCA CTGGAACATG GAACCCTAAG GGCCCCGATC 
     2581 TTAGCAACTG TACCTCACAC TGGGTGAATC AGCTGGCTCA GAAGATCAGA AGCGGAGAAA 
     2641 ATGCTGCTAG TCTTGCCAAT GAACTGGCTA AACATACCAA AGGGCCAGTG TTTGCTGGGG 
     2701 ATGTAAGTTC TTCAGTGAGA TTGATGGAGC AGTTGGTGGA CATCCTTGAT GCACAGCTGC 
     2761 AGGAACTGAA ACCTAGTGAA AAAGATTCAG CTGGACGGAG TTATAACAAG GCAATTGTTG 
     2821 ACACAGTGGA CAACCTTCTG AGACCCGAAG CTTTGGAATC ATGGAAACAT ATGAATTCTT 
     2881 CTGAACAAGC ACATACTGCA ACAATGTTAC TCGATACATT GGAAGAAGGA GCTTTTGTCC 
     2941 TAGCTGACAA TCTTTTAGAA CCAACAAGGG TCTCAATGCC CACAGAAAAT ATTGTCCTGG 
     3001 AAGTTGCCGT ACTCAGTACA GAAGGACAGA TCCAAGACTT TAAATTTCCT CTGGGCATCA 
     3061 AAGGAGCAGG CAGCTCAATC CAACTGTCCG CAAATACCGT CAAACAGAAC AGCAGGAATG 
     3121 GGCTTGCAAA GTTGGTGTTC ATCATTTACC GGAGCCTGGG ACAGTTCCTT AGTACAGAAA 
     3181 ATGCAACCAT TAAACTGGGT GCTGATTTTA TTGGTCGTAA TAGCACCATT GCAGTGAACT 
     3241 CTCACGTCAT TTCAGTTTCA ATCAATAAAG AGTCCAGCCG AGTATACCTG ACTGATCCTG 
     3301 TGCTTTTTAC CCTGCCACAC ATTGATCCTG ACAATTATTT CAATGCAAAC TGCTCCTTCT 
     3361 GGAACTACTC AGAGAGAACT ATGATGGGAT ATTGGTCTAC CCAGGGCTGC AAGCTGGTTG 
     3421 ACACTAATAA AACTCGAACA ACGTGTGCAT GCAGCCACCT AACCAATTTT GCAATTCTCA 
     3481 TGGCCCACAG GGAAATTGCA TATAAAGATG GCGTTCATGA ATTACTTCTT ACAGTCATCA 
     3541 CCTGGGTGGG AATTGTCATT TCCCTTGTTT GCCTGGCTAT CTGCATCTTC ACCTTCTGCT 
     3601 TTTTCCGTGG CCTACAGAGT GACCGAAATA CTATTCACAA GAACCTTTGT ATCAACCTTT 
     3661 TCATTGCTGA ATTTATTTTC CTAATAGGCA TTGATAAGAC AAAATATGCG ATTGCATGCC 
     3721 CAATATTTGC AGGACTTCTA CACTTTTTCT TTTTGGCAGC TTTTGCTTGG ATGTGCCTAG 
     3781 AAGGTGTGCA GCTCTACCTA ATGTTAGTTG AAGTTTTTGA AAGTGAATAT TCAAGGAAAA 
     3841 AATATTACTA TGTTGCTGGT TACTTGTTTC CTGCCACAGT GGTTGGAGTT TCAGCTGCTA 
     3901 TTGACTATAA GAGCTATGGA ACAGAAAAAG CTTGCTGGCT TCATGTTGAT AACTACTTTA 
     3961 TATGGAGCTT CATTGGACCT GTTACCTTCA TTATTCTGCT AAATATTATC TTCTTGGTGA 
     4021 TCACATTGTG CAAAATGGTG AAGCATTCAA ACACTTTGAA ACCAGATTCT AGCAGGTTGG 
     4081 AAAACATTAA GTCTTGGGTG CTTGGCGCTT TCGCTCTTCT GTGTCTTCTT GGCCTCACCT 
     4141 GGTCCTTTGG GTTGCTTTTT ATTAATGAGG AGACTATTGT GATGGCATAT CTCTTCACTA 
     4201 TATTTAATGC TTTCCAGGGA GTGTTCATTT TCATCTTTCA CTGTGCTCTC CAAAAGAAAG 
     4261 TACGAAAAGA ATATGGCAAG TGCTTCAGAC ACTCATACTG CTGTGGAGGC CTCCCAACTG 
     4321 AGAGTCCCCA CAGTTCAGTG AAGGCATCAA CCACCAGAAC CAGTGCTCGC TATTCCTCTG 
     4381 GCACACAGAG TCGTATAAGA AGAATGTGGA ATGATACTGT GAGAAAACAA TCAGAATCTT 
     4441 CTTTTATCTC AGGTGACATC AATAGCACTT CAACACTTAA TCAAGGACTG ACATCACATG 
     4501 GTCTGAGAGC CCATCTTCAA GATTTATATC ATTTAGAGCT ACTCTTAGGC CAGATAGCCA 
     4561 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4621 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4681 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4741 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4801 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4861 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4921 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4981 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5041 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5101 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5161 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5221 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5281 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5341 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5401 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5461 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5521 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5581 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5641 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5701 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5761 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5821 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5881 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5941 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6001 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6061 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6121 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6181 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6241 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6301 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6361 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6421 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6481 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6541 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6601 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6661 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6721 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6781 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6841 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6901 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6961 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7021 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7081 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7141 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7201 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7261 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7321 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7381 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7441 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7501 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7561 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7621 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7741 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7801 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7861 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7921 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7981 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8041 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8101 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8161 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8221 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8281 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8341 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8401 AACGCGAATT TT
//