LOCUS       RC240020                8391 bp    DNA     circular  SYN 
DEFINITION  PLCB2 (myc-DDK-tagged) - Human phospholipase C, beta 2 (PLCB2), transcript variant 3
ACCESSION   NM_001284298
VERSION     NM_001284298.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4548..4639 
                     /label="Myc-DDK"  
     misc_feature    4669..5254 
                     /label="PolyA signal"  
     misc_feature    4726..4745 
                     /label="Restriction Enzyme"  
     misc_feature    5530..6202 
                     /label="ColE1"  
     misc_feature    6632..7426 
                     /label="Kan/Neo"  
     misc_feature    7461..7763 
                     /label="SV40 ori"  
     misc_feature    7849..8326 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4530..4536 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4530 
                     /label="PLCB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCTGCTC AACCCTGTCC TGCTGCCCCC CAAGGTGAAG GCCTATCTGA 
     1081 GCCAAGGGGA GCGCTTCATC AAATGGGATG ATGAAACTAC AGTTGCCTCT CCAGTTATCC 
     1141 TCCGTGTGGA TCCTAAGGGC TACTACTTAT ACTGGACGTA TCAAAGTAAG GAGATGGAGT 
     1201 TTCTGGATAT CACCAGCATC CGGGATACTC GCTTTGGGAA GTTTGCCAAG ATGCCCAAGA 
     1261 GCCAGAAGCT CCGGGACGTC TTCAACATGG ACTTTCCTGA TAACAGTTTC CTGCTGAAGA 
     1321 CACTCACGGT GGTGTCCGGC CCGGACATGG TGGACCTCAC CTTCCACAAC TTCGTCTCCT 
     1381 ACAAGGAGAA CGTGGGCAAG GCCTGGGCTG AGGACGTACT GGCCCTAGTC AAACATCCGC 
     1441 TGACGGCCAA CGCCTCCCGC AGCACCTTCC TGGACAAGAT CCTTGTGAAG CTCAAGATGC 
     1501 AGCTCAACTC TGAAGGGAAG ATTCCGGTGA AGAACTTTTT CCAGATGTTT CCTGCTGACC 
     1561 GCAAGCGGGT GGAAGCTGCT CTCAGTGCCT GCCACCTCCC CAAAGGCAAA AATGACGCCA 
     1621 TCAATCCTGA GGACTTCCCA GAACCTGTCT ACAAGAGTTT CCTCATGAGC CTCTGTCCTC 
     1681 GGCCAGAAAT AGATGAGATC TTCACTTCTT ACCATGCTAA GGCCAAACCC TACATGACGA 
     1741 AGGAGCACCT GACCAAATTC ATCAACCAGA AACAGCGGGA CTCCCGGCTT AACTCCCTGC 
     1801 TGTTCCCGCC AGCACGGCCT GACCAGGTGC AGGGCCTCAT CGACAAGTAT GAGCCCAGTG 
     1861 GCATCAATGC ACAGAGGGGC CAGCTGTCAC CTGAAGGCAT GGTCTGGTTT CTCTGTGGGC 
     1921 CAGAGAACAG CGTGCTGGCC CAGGACAAGC TGCTGCTCCA CCACGACATG ACGCAGCCAC 
     1981 TCAATCATTA CTTCATCAAC TCGTCCCACA ACACCTACCT GACAGCCGGC CAGTTCTCAG 
     2041 GCCTCTCCTC GGCTGAGATG TACCGCCAGG TGCTGCTCTC TGGCTGCCGT TGCGTGGAGC 
     2101 TAGACTGCTG GAAGGGGAAA CCCCCTGACG AGGAGCCCAT TATCACCCAT GGCTTCACCA 
     2161 TGACCACAGA CATCTTCTTC AAAGAAGCAA TTGAGGCTAT TGCAGAAAGC GCCTTTAAGA 
     2221 CCTCCCCCTA TCCCATCATC CTGTCGTTTG AGAACCATGT GGACTCACCC CGCCAGCAGG 
     2281 CTAAGATGGC TGAGTATTGC CGGACGATCT TTGGGGATAT GCTGCTCACA GAGCCCCTGG 
     2341 AAAAGTTCCC ACTAAAACCA GGTGTCCCCC TGCCCAGCCC TGAGGATCTC AGGGGCAAGA 
     2401 TCCTCATCAA GAACAAGAAG AACCAGTTTT CTGGCCCCAC CTCCTCCAGT AAGGATACTG 
     2461 GTGGGGAGGC TGAGGGCAGC AGCCCACCCA GTGCCCCTGC AGGTGAGGGC ACAGTGTGGG 
     2521 CTGGCGAGGA AGGGACTGAG CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT 
     2581 CAGGAAACCT GGATGAAGAA GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC 
     2641 TGGAAGTGAC GGCTTATGAG GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT 
     2701 TCGTCTCCTT TGAGTTCTCT GCCCAAAAGA ACCGAAGTTA TGTCATCTCG TCCTTCACAG 
     2761 AGCTCAAGGC ATATGACCTG CTCTCCAAGG CCTCGGTGCA GTTTGTGGAC TACAACAAGC 
     2821 GCCAGATGAG CCGCATTTAC CCCAAGGGAA CCCGCATGGA CTCCTCCAAC TACATGCCCC 
     2881 AGATGTTCTG GAATGCTGGA TGCCAGATGG TTGCCCTCAA CTTCCAGACG ATGGACTTGC 
     2941 CCATGCAGCA GAACATGGCA GTATTTGAGT TCAACGGGCA GAGCGGCTAC CTCCTCAAGC 
     3001 ATGAGTTCAT GCGCCGGCCG GACAAGCAGT TCAACCCCTT CTCAGTGGAC CGCATCGACG 
     3061 TGGTGGTGGC CACCACCCTT TCCATTACGG TGATCTCTGG GCAGTTCCTG TCAGAACGCA 
     3121 GCGTGCGCAC CTATGTAGAA GTGGAGCTGT TTGGCCTTCC TGGGGACCCC AAGAGGCGCT 
     3181 ATCGAACTAA GCTGTCACCC AGTACTAACT CCATCAATCC TGTCTGGAAG GAGGAGCCCT 
     3241 TTGTCTTTGA GAAGATCTTG ATGCCTGAGC TGGCCTCCCT CAGAGTGGCT GTGATGGAGG 
     3301 AAGGCAACAA GTTTCTTGGA CACCGCATCA TCCCCATCAA TGCCCTAAAT TCTGGGTACC 
     3361 ACCACCTGTG CCTGCACAGT GAGAGCAACA TGCCCCTCAC CATGCCTGCG CTCTTCATCT 
     3421 TCCTGGAGAT GAAGGACTAC ATACCTGGTG CTTGGGCAGA TCTCACTGTG GCCCTCGCCA 
     3481 ACCCCATTAA GTTCTTCAGT GCCCATGACA CGAAGTCTGT GAAGCTCAAG GAGGCCATGG 
     3541 GAGGTCTGCC TGAGAAGCCC TTCCCACTGG CGAGTCCAGT TGCCAGCCAG GTCAATGGGG 
     3601 CGTTGGCCCC AACGAGCAAT GGGTCACCAG AGCCGCGGAC CGCCAGCCTG GAGGAGCTCC 
     3661 GGGAGCTAAA GGGCGTGGTG AAGCTGCAGC GGCGGCACGA GAAGGAGCTG CGAGAGTTGG 
     3721 AGCGGCGCGG AGCGCGGCGC TGGGAGGAGC TGCTGCAGCG GGGCGCGGCG CAGCTGGCGG 
     3781 AGCTCGGGCC ACCGGGCGTG GGGGGCGTCG GGGCCTGCAA GCTCGGTCCC GGCAAGGGCT 
     3841 CTCGCAAGAA GAGGAGCCTG CCCCGCGAGG AGAGCGCCGG AGCCGCGCCG GGCGAGGGCC 
     3901 CTGAGGGCGT GGACGGGCGC GTGCGGGAGC TGAAAGACAG GCTGGAGCTG GAGCTGCTGC 
     3961 GGCAGGGCGA GGAGCAGTAC GAGTGCGTTC TGAAGCGCAA GGAGCAGCAC GTGGCCGAGC 
     4021 AAATCTCCAA AATGATGGAG CTGGCCAGAG AGAAACAGGC GGCAGAGCTG AAGGCCCTGA 
     4081 AGGAGACGTC GGAGAACGAC ACCAAAGAGA TGAAGAAAAA GCTGGAGACA AAGAGACTGG 
     4141 AGCGGATCCA GGGCATGACC AAAGTCACCA CAGACAAGAT GGCCCAGGAG AGGTTGAAGA 
     4201 GAGAGATTAA CAACTCCCAC ATCCAGGAAG TAGTGCAGGT GATCAAGCAG ATGACGGAGA 
     4261 ACTTGGAGAG GCACCAGGAG AAGCTGGAGG AGAAGCAGGC GGCTTGCCTG GAACAGATAC 
     4321 GGGAGATGGA AAAGCAGTTC CAGAAGGAGG CGCTGGCAGA GTACGAGGCC AGGATGAAGG 
     4381 GTCTGGAGGC AGAGGTGAAG GAGTCGGTGA GGGCCTGCCT CAGGACCTGC TTTCCCTCCG 
     4441 AGGCCAAGGA CAAGCCTGAG AGGGCCTGCG AGTGCCCCCC AGAGCTGTGT GAGCAGGACC 
     4501 CACTCATAGC AAAGGCAGAT GCCCAGGAGA GCCGCCTCAC GCGTACGCGG CCGCTCGAGC 
     4561 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4621 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4681 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4741 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4801 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4861 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4921 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4981 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5041 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5101 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5161 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5221 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5281 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5341 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5401 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5461 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5521 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5581 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5641 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5701 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5761 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5821 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5881 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5941 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6001 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6061 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6121 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6181 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6241 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6301 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6361 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6421 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6481 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6541 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6601 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6661 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6721 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6781 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6841 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6901 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6961 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7021 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7081 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7141 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7201 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7261 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7321 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7381 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7441 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7501 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7561 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7621 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7801 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7861 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7921 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7981 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8041 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8101 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8161 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8221 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8281 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8341 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//