LOCUS       RC240006                8316 bp    DNA     circular  SYN 
DEFINITION  ACLY (myc-DDK-tagged) - Human ATP citrate lyase (ACLY), transcript variant 4
ACCESSION   NM_001303275
VERSION     NM_001303275.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4473..4564 
                     /label="Myc-DDK"  
     misc_feature    4594..5179 
                     /label="PolyA signal"  
     misc_feature    4651..4670 
                     /label="Restriction Enzyme"  
     misc_feature    5455..6127 
                     /label="ColE1"  
     misc_feature    6557..7351 
                     /label="Kan/Neo"  
     misc_feature    7386..7688 
                     /label="SV40 ori"  
     misc_feature    7774..8251 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4455..4461 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4455 
                     /label="ACLY"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCGGGG AAAAGTCCGG CTGGGCCGGG ACAAAAGCCG GATCCCGGGA 
     1081 AGCTACCGGC TGCTGGGGTG CTCCGGATTT TGCGGGGTTC GTCGGGCCTG TGGAAGAAGC 
     1141 GCCGCGCACG GACTTCGGCA GAGACAGGTA GAGCAGGTCT CTCTGCAGCC ATGTCGGCCA 
     1201 AGGCAATTTC AGAGCAGACG GGCAAAGAAC TCCTTTACAA GTTCATCTGT ACCACCTCAG 
     1261 CCATCCAGAA TCGGTTCAAG TATGCTCGGG TCACTCCTGA CACAGACTGG GCCCGCTTGC 
     1321 TGCAGGACCA CCCCTGGCTG CTCAGCCAGA ACTTGGTAGT CAAGCCAGAC CAGCTGATCA 
     1381 AACGTCGTGG AAAACTTGGT CTCGTTGGGG TCAACCTCAC TCTGGATGGG GTCAAGTCCT 
     1441 GGCTGAAGCC ACGGCTGGGA CAGGAAGCCA CAGTTGGCAA GGCCACAGGC TTCCTCAAGA 
     1501 ACTTTCTGAT CGAGCCCTTC GTCCCCCACA GTCAGGCTGA GGAGTTCTAT GTCTGCATCT 
     1561 ATGCCACCCG AGAAGGGGAC TACGTCCTGT TCCACCACGA GGGGGGTGTG GACGTGGGTG 
     1621 ATGTGGACGC CAAGGCCCAG AAGCTGCTTG TTGGCGTGGA TGAGAAACTG AATCCTGAGG 
     1681 ACATCAAAAA ACACCTGTTG GTCCACGCCC CTGAAGACAA GAAAGAAATT CTGGCCAGTT 
     1741 TTATCTCCGG CCTCTTCAAT TTCTACGAGG ACTTGTACTT CACCTACCTC GAGATCAATC 
     1801 CCCTTGTAGT GACCAAAGAT GGAGTCTATG TCCTTGACTT GGCGGCCAAG GTGGACGCCA 
     1861 CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATCGA GTTCCCTCCC CCCTTCGGGC 
     1921 GGGAGGCATA TCCAGAGGAA GCCTACATTG CAGACCTCGA TGCCAAAAGT GGGGCAAGCC 
     1981 TGAAGCTGAC CTTGCTGAAC CCCAAAGGGA GGATCTGGAC CATGGTGGCC GGGGGTGGCG 
     2041 CCTCTGTCGT GTACAGCGAT ACCATCTGTG ATCTAGGGGG TGTCAACGAG CTGGCAAACT 
     2101 ATGGGGAGTA CTCAGGCGCC CCCAGCGAGC AGCAGACCTA TGACTATGCC AAGACTATCC 
     2161 TCTCCCTCAT GACCCGAGAG AAGCACCCAG ATGGCAAGAT CCTCATCATT GGAGGCAGCA 
     2221 TCGCAAACTT CACCAACGTG GCTGCCACGT TCAAGGGCAT CGTGAGAGCA ATTCGAGATT 
     2281 ACCAGGGCCC CCTGAAGGAG CACGAAGTCA CAATCTTTGT CCGAAGAGGT GGCCCCAACT 
     2341 ATCAGGAGGG CTTACGGGTG ATGGGAGAAG TCGGGAAGAC CACTGGGATC CCCATCCATG 
     2401 TCTTTGGCAC AGAGACTCAC ATGACGGCCA TTGTGGGCAT GGCCCTGGGC CACCGGCCCA 
     2461 TCCCCAACCA GCCACCCACA GCGGCCCACA CTGCAAACTT CCTCCTCAAC GCCAGCGGGA 
     2521 GCACATCGAC GCCAGCCCCC AGCAGGACAG CATCTTTTTC TGAGTCCAGG GCCGATGAGG 
     2581 TGGCGCCTGC AAAGAAGGCC AAGCCTGCCA TGCCACAAGG AAAGAGCACC ACCCTCTTCA 
     2641 GCCGCCACAC CAAGGCCATT GTGTGGGGCA TGCAGACCCG GGCCGTGCAA GGCATGCTGG 
     2701 ACTTTGACTA TGTCTGCTCC CGAGACGAGC CCTCAGTGGC TGCCATGGTC TACCCTTTCA 
     2761 CTGGGGACCA CAAGCAGAAG TTTTACTGGG GGCACAAAGA GATCCTGATC CCTGTCTTCA 
     2821 AGAACATGGC TGATGCCATG AGGAAGCATC CGGAGGTAGA TGTGCTCATC AACTTTGCCT 
     2881 CTCTCCGCTC TGCCTATGAC AGCACCATGG AGACCATGAA CTATGCCCAG ATCCGGACCA 
     2941 TCGCCATCAT AGCTGAAGGC ATCCCTGAGG CCCTCACGAG AAAGCTGATC AAGAAGGCGG 
     3001 ACCAGAAGGG AGTGACCATC ATCGGACCTG CCACTGTTGG AGGCATCAAG CCTGGGTGCT 
     3061 TTAAGATTGG CAACACAGGT GGGATGCTGG ACAACATCCT GGCCTCCAAA CTGTACCGCC 
     3121 CAGGCAGCGT GGCCTATGTC TCACGTTCCG GAGGCATGTC CAACGAGCTC AACAATATCA 
     3181 TCTCTCGGAC CACGGATGGC GTCTATGAGG GCGTGGCCAT TGGTGGGGAC AGGTACCCGG 
     3241 GCTCCACATT CATGGATCAT GTGTTACGCT ATCAGGACAC TCCAGGAGTC AAAATGATTG 
     3301 TGGTTCTTGG AGAGATTGGG GGCACTGAGG AATATAAGAT TTGCCGGGGC ATCAAGGAGG 
     3361 GCCGCCTCAC TAAGCCCATC GTCTGCTGGT GCATCGGGAC GTGTGCCACC ATGTTCTCCT 
     3421 CTGAGGTCCA GTTTGGCCAT GCTGGAGCTT GTGCCAACCA GGCTTCTGAA ACTGCAGTAG 
     3481 CCAAGAACCA GGCTTTGAAG GAAGCAGGAG TGTTTGTGCC CCGGAGCTTT GATGAGCTTG 
     3541 GAGAGATCAT CCAGTCTGTA TACGAAGATC TCGTGGCCAA TGGAGTCATT GTACCTGCCC 
     3601 AGGAGGTGCC GCCCCCAACC GTGCCCATGG ACTACTCCTG GGCCAGGGAG CTTGGTTTGA 
     3661 TCCGCAAACC TGCCTCGTTC ATGACCAGCA TCTGCGATGA GCGAGGACAG GAGCTCATCT 
     3721 ACGCGGGCAT GCCCATCACT GAGGTCTTCA AGGAAGAGAT GGGCATTGGC GGGGTCCTCG 
     3781 GCCTCCTCTG GTTCCAGAAA AGGTTGCCTA AGTACTCTTG CCAGTTCATT GAGATGTGTC 
     3841 TGATGGTGAC AGCTGATCAC GGGCCAGCCG TCTCTGGAGC CCACAACACC ATCATTTGTG 
     3901 CGCGAGCTGG GAAAGACCTG GTCTCCAGCC TCACCTCGGG GCTGCTCACC ATCGGGGATC 
     3961 GGTTTGGGGG TGCCTTGGAT GCAGCAGCCA AGATGTTCAG TAAAGCCTTT GACAGTGGCA 
     4021 TTATCCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATTG 
     4081 GTCACCGAGT GAAGTCGATA AACAACCCAG ACATGCGAGT GCAGATCCTC AAAGATTACG 
     4141 TCAGGCAGCA CTTCCCTGCC ACTCCTCTGC TCGATTATGC ACTGGAAGTA GAGAAGATTA 
     4201 CCACCTCGAA GAAGCCAAAT CTTATCCTGA ATGTAGATGG TCTCATCGGA GTCGCATTTG 
     4261 TAGACATGCT TAGAAACTGT GGGTCCTTTA CTCGGGAGGA AGCTGATGAA TATATTGACA 
     4321 TTGGAGCCCT CAATGGCATC TTTGTGCTGG GAAGGAGTAT GGGGTTCATT GGACACTATC 
     4381 TTGATCAGAA GAGGCTGAAG CAGGGGCTGT ATCGTCATCC GTGGGATGAT ATTTCATATG 
     4441 TTCTTCCGGA ACACATGAGC ATGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4501 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4561 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4621 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4681 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4741 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4801 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4861 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4921 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4981 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5041 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5101 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5161 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5221 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5281 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5341 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5401 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5461 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5521 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5581 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5641 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5701 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5761 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5821 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5881 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5941 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6001 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6061 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6121 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6181 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6241 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6301 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6361 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6421 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6481 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6541 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6601 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6661 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6721 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6781 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6841 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6901 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6961 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7021 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7081 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7141 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7201 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7261 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7321 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7381 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7441 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7501 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7561 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7741 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7801 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7861 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7921 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7981 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8041 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8101 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8161 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8221 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8281 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//