LOCUS       RC235281                8973 bp    DNA     circular  SYN 
DEFINITION  MED23 (Myc-DDK tagged) - Homo sapiens mediator complex subunit 23 (MED23), transcript variant 4
ACCESSION   NM_001270522
VERSION     NM_001270522.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5130..5221 
                     /label="Myc-DDK"  
     misc_feature    5251..5836 
                     /label="PolyA signal"  
     misc_feature    5308..5327 
                     /label="Restriction Enzyme"  
     misc_feature    6112..6784 
                     /label="ColE1"  
     misc_feature    7214..8008 
                     /label="Kan/Neo"  
     misc_feature    8043..8345 
                     /label="SV40 ori"  
     misc_feature    8431..8908 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5112..5118 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5112 
                     /label="MED23"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGACGCAA CTGCAGAGCA TTTTCGAAGA GGTGGTGAAA ACGGAAGTTA 
     1081 TAGAAGAGGC TTTTCCTGGC ATGTTTATGG ATACTCCTGA AGATGAGAAA ACAAAACTAA 
     1141 TTAGCTGTTT GGGGGCCTTC AGACAGTTTT GGGGTGGTCT TTCTCAGGAG TCTCATGAAC 
     1201 AGTGTATCCA GTGGATTGTT AAGTTTATTC ATGGTCAGCA TAGTCCTAAA AGAATTTCTT 
     1261 TTCTTTATGA CTGCTTAGCA ATGGCAGTTG AGACTGGTCT CCTTCCACCC AGGCTGGTTT 
     1321 GTGAATCCCT GATAAACTCT GACACTCTTG AGTGGGAAAG AACACAGCTT TGGGCCTTAA 
     1381 CATTTAAACT GGTTCGGAAA ATAATTGGGG GAGTGGATTA CAAGGGTGTT CGAGATCTCT 
     1441 TAAAAGTGAT TTTGGAGAAG ATTTTGACAA TTCCTAATAC AGTGAGCTCT GCTGTTGTAC 
     1501 AGCAGCTTCT GGCAGCAAGA GAGGTTATAG CATATATCTT GGAAAGAAAT GCCTGCTTAT 
     1561 TACCAGCCTA TTTTGCAGTC ACTGAGATCA GGAAACTGTA TCCTGAAGGC AAACTTCCAC 
     1621 ACTGGTTACT TGGAAACCTA GTATCAGACT TTGTGGATAC CTTCAGGCCC ACAGCAAGGA 
     1681 TAAACTCCAT TTGTGGTCGC TGTAGTCTTC TGCCAGTTGT AAATAATTCG GGTGCCATTT 
     1741 GTAATTCATG GAAACTGGAT CCTGCTACTC TTCGTTTTCC TTTGAAAGGC CTTTTGCCAT 
     1801 ATGATAAGGA TCTGTTTGAA CCACAGACTG CTTTGTTGAG ATATGTATTG GAGCAGCCTT 
     1861 ATTCCAGGGA TATGGTCTGC AATATGCTAG GTTTAAATAA GCAGCACAAG CAGCGCTGCC 
     1921 CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA TCTGAGACCG 
     1981 AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC TCAAGTCAGC 
     2041 TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT TCTCTTCATC 
     2101 AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG GTTCTCCTGC 
     2161 AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT GTGATGAAGC 
     2221 TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT AACAAACCCC 
     2281 AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA AAAGCTCAAA 
     2341 ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC CATGAGTTCC 
     2401 TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT GCTCTATTGT 
     2461 GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT CTGGTAGAAA 
     2521 CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT ATGGCTTCAG 
     2581 GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT GCCAAAATGA 
     2641 GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG TCCAGTGTGG 
     2701 CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG GAAATAGAGT 
     2761 CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA TCACATGCAT 
     2821 GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT ATTCAGCCTC 
     2881 ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA CAAACAAACC 
     2941 AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA GCATTAGGTA 
     3001 GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA GTGCTCTCAG 
     3061 CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA ACTCATGTAA 
     3121 CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC ATACTTCAGA 
     3181 CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT TTTCCAGGCC 
     3241 CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT AATCTGAAAA 
     3301 AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC GACATTATTA 
     3361 CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG AAAATGCTCT 
     3421 TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT GGAGCCAGGG 
     3481 CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT TCTACATCAG 
     3541 CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA TGGAAGTATA 
     3601 ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC GAAGGAAATG 
     3661 AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC GATTTTAGAA 
     3721 ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG AATGACTGGC 
     3781 ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT GAGGGCCTCG 
     3841 CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC TATTTTGGGA 
     3901 ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT TTAGAGTTGC 
     3961 TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA TATAAATTTC 
     4021 ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG CACCTGAGAG 
     4081 ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG AAGGATAATC 
     4141 GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT GCACGAGAGG 
     4201 AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA CTAGTCGATA 
     4261 CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC AATGAGTTTC 
     4321 CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC TTGGCAGTTT 
     4381 CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG CCTTTAGTGC 
     4441 CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT GCCCTACCAG 
     4501 AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC CCCAGCTTGA 
     4561 CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT GCCTGTCATC 
     4621 AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT GTGTGGCACC 
     4681 ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA CTTCTTCCTA 
     4741 TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA TTTTTACAAA 
     4801 GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT TATGACATGC 
     4861 TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC TGTGACTTCC 
     4921 TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA GAGAAGATTA 
     4981 TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC ATTAGCAAGA 
     5041 TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA CCTCAGTCTA 
     5101 ATCAGGTTGA CACTCTCACC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5161 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5221 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5281 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5341 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5401 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5461 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5521 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5581 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5641 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5701 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5761 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5821 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5881 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5941 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6001 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6061 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6121 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6181 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6241 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6301 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6361 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6421 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6481 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6541 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6601 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6661 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6721 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6781 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6841 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6901 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6961 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7021 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7081 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7141 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7201 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7261 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7321 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7381 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7441 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7501 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7561 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7621 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7681 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7741 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7801 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7861 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7921 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7981 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8041 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8101 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8161 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8221 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8281 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8341 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8401 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8461 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8521 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8581 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8641 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8701 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8761 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8821 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8881 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8941 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//