LOCUS       RC235242                8622 bp    DNA     circular  SYN 
DEFINITION  PPFIA2 (Myc-DDK tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 2
ACCESSION   NM_001220473
VERSION     NM_001220473.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4761 
                     /label="PPFIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTGAA GTGATGCCCA CGATTAATGA GGACACCCCA ATGAGCCAAA 
     1081 GGGGGTCCCA AAGCAGTGGC TCGGACTCAG ACTCCCATTT TGAGCAGCTG ATGGTGAATA 
     1141 TGCTAGATGA AAGGGATCGT CTTCTAGACA CCCTTCGGGA GACCCAGGAA AGCCTCTCAC 
     1201 TTGCCCAGCA AAGACTTCAG GATGTCATCT ATGACCGAGA CTCACTCCAG AGACAGCTCA 
     1261 ATTCAGCCCT GCCACAGGAT ATCGAATCCC TAACAGGAGG GCTGGCTGGT TCTAAGGGGG 
     1321 CTGATCCACC GGAATTTGCT GCACTGACAA AAGAATTAAA TGCCTGCAGG GAACAACTTC 
     1381 TAGAAAAGGA AGAAGAAATC TCTGAACTTA AAGCTGAAAG AAACAACACA AGACTATTAC 
     1441 TGGAGCATTT GGAGTGCCTT GTGTCACGAC ATGAAAGATC ACTAAGAATG ACGGTGGTAA 
     1501 AACGGCAAGC CCAGTCTCCC TCAGGAGTAT CCAGTGAAGT TGAAGTTCTC AAGGCACTGA 
     1561 AATCTTTGTT TGAGCACCAC AAGGCCTTGG ATGAAAAGGT AAGGGAGCGA CTGAGGGTTT 
     1621 CTTTAGAAAG AGTCTCTGCA CTGGAAGAAG AACTAGCTGC TGCTAATCAG GAGATTGTTG 
     1681 CCTTGCGTGA ACAAAATGTT CATATACAAA GAAAAATGGC ATCAAGCGAG GGATCCACAG 
     1741 AGTCAGAACA TCTTGAAGGG ATGGAACCTG GACAGAAAGT CCATGAGAAG CGTTTGTCCA 
     1801 ATGGTTCTAT AGACTCAACC GATGAAACTA GTCAAATAGT TGAACTACAA GAATTGCTTG 
     1861 AAAAGCAAAA CTATGAAATG GCCCAGATGA AAGAACGTTT AGCAGCCCTT TCTTCCCGAG 
     1921 TGGGAGAGGT GGAACAGGAA GCAGAGACAG CAAGAAAGGA TCTCATTAAA ACAGAAGAAA 
     1981 TGAACACCAA GTATCAAAGG GACATTAGGG AGGCCATGGC ACAAAAGGAA GATATGGAAG 
     2041 AAAGAATTAC AACCCTTGAA AAGCGTTACC TCAGTGCTCA GAGAGAATCT ACCTCCATAC 
     2101 ATGACATGAA TGATAAACTA GAAAATGAGT TAGCAAATAA AGAAGCTATC CTGCGGCAGA 
     2161 TGGAAGAGAA AAACAGACAG TTACAAGAAC GTCTTGAGCT AGCTGAACAA AAGTTGCAGC 
     2221 AGACCATGAG AAAGGCTGAA ACCTTGCCTG AAGTAGAGGC TGAACTGGCT CAGAGAATTG 
     2281 CAGCCCTAAC CAAGGCTGAA GAGAGACATG GAAATATTGA AGAACGTATG AGACATTTAG 
     2341 AGGGTCAACT TGAAGAGAAG AATCAAGAAC TTCAAAGAGC TAGGCAAAGA GAGAAAATGA 
     2401 ATGAGGAGCA TAACAAGAGA TTATCGGATA CGGTTGATAG ACTTCTGACT GAATCCAATG 
     2461 AACGCCTACA ACTACACTTA AAGGAAAGAA TGGCTGCTCT AGAAGAAAAG AATGTTTTAA 
     2521 TTCAAGAATC AGAAACTTTC AGAAAGAATC TTGAAGAATC TTTACATGAT AAGGAAAGAT 
     2581 TAGCAGAAGA AATTGAAAAG CTGAGATCTG AACTTGACCA ATTGAAAATG AGAACTGGCT 
     2641 CTTTAATTGA ACCCACAATA CCAAGAACTC ATCTAGACAC CTCAGCTGAG TTGCGGTACT 
     2701 CAGTGGGATC CCTAGTGGAC AGCCAGTCTG ATTACAGAAC AACTAAAGTA ATAAGAAGAC 
     2761 CAAGGAGAGG CCGCATGGGT GTGCGAAGAG ATGAGCCAAA GGTGAAATCT CTTGGGGATC 
     2821 ACGAGTGGAA TAGAACTCAA CAGATTGGAG TACTAAGCAG CCACCCTTTT GAAAGTGACA 
     2881 CTGAAATGTC TGATATTGAT GATGATGACA GAGAAACAAT TTTTAGCTCA ATGGATCTTC 
     2941 TCTCTCCAAG TGGTCATTCC GATGCCCAGA CGCTAGCCAT GATGCTTCAG GAACAATTGG 
     3001 ATGCCATCAA CAAAGAAATC AGGCTAATTC AGGAAGAAAA AGAATCTACA GAGTTGCGTG 
     3061 CTGAAGAAAT TGAAAATAGA GTGGCTAGTG TGAGCCTCGA AGGCCTGAAT TTGGCAAGGG 
     3121 TCCACCCAGG TACCTCCATT ACTGCCTCTG TTACAGCTTC ATCGCTGGCC AGTTCATCTC 
     3181 CCCCCAGTGG ACACTCAACT CCAAAGCTCA CCCCTCGAAG CCCTGCCAGG GAAATGGATC 
     3241 GGATGGGAGT CATGACACTG CCAAGTGATC TGAGGAAACA TCGGAGAAAG ATTGCAGTTG 
     3301 TGGAAGAAGA TGGTCGAGAG GACAAAGCAA CAATTAAATG TGAAACTTCT CCTCCTCCTA 
     3361 CCCCTAGAGC CCTCAGAATG ACTCACACTC TCCCTTCTTC CTACCACAAT GATGCTCGAA 
     3421 GTAGTTTATC TGTCTCTCTT GAGCCAGAAA GCCTCGGGCT TGGTAGTGCC AACAGCAGCC 
     3481 AAGACTCTCT TCACAAAGCC CCCAAGAAGA AAGGAATCAA GTCTTCAATA GGACGTTTGT 
     3541 TTGGTAAAAA AGAAAAAGCT CGACTTGGGC AGCTCCGAGG CTTTATGGAG ACTGAAGCTG 
     3601 CAGCTCAGGA GTCCCTGGGG TTAGGCAAAC TCGGAACTCA AGCTGAGAAG GATCGAAGAC 
     3661 TAAAGAAAAA GCATGAACTT CTTGAAGAAG CTCGGAGAAA GGGATTACCT TTTGCCCAGT 
     3721 GGGATGGGCC AACTGTGGTC GCATGGCTAG AGCTTTGGTT GGGAATGCCT GCGTGGTACG 
     3781 TGGCAGCCTG CCGAGCCAAC GTGAAGAGTG GTGCCATCAT GTCTGCTTTA TCTGACACTG 
     3841 AGATCCAGAG AGAAATTGGA ATCAGCAATC CACTGCATCG CTTAAAACTT CGATTAGCAA 
     3901 TCCAGGAGAT GGTTTCCCTA ACAAGTCCTT CAGCTCCTCC AACATCTCGA ACTCCTTCAG 
     3961 GCAACGTTTG GGTGACTCAT GAAGAAATGG AAAATCTTGC AGCTCCAGCA AAAACGAAAG 
     4021 AATCTGAGGA AGGAAGCTGG GCCCAGTGTC CGGTTTTTCT ACAGACCCTG GCTTATGGAG 
     4081 ATATGAATCA TGAGTGGATT GGAAATGAAT GGCTTCCCAG CTTGGGGTTA CCTCAGTACA 
     4141 GAAGTTACTT TATGGAATGC TTGGTAGATG CAAGAATGTT AGATCACCTA ACAAAAAAAG 
     4201 ATCTCCGTGT CCATTTAAAA ATGGTGGATA GTTTCCATCG AACAAGTTTA CAATATGGAA 
     4261 TTATGTGCTT AAAGAGGTTG AATTATGACA GAAAAGAACT AGAAAGAAGA CGGGAAGCAA 
     4321 GCCAACATGA AATAAAAGAC GTGTTGGTGT GGAGCAATGA CCGAGTTATT CGCTGGATAC 
     4381 AAGCAATTGG ACTTCGAGAA TATGCAAATA ATATACTTGA GAGCGGTGTG CATGGCTCAC 
     4441 TTATAGCCCT GGATGAAAAC TTTGACTACA GCAGCTTAGC TTTATTATTA CAGATTCCAA 
     4501 CACAGAACAC CCAGGCAAGG CAGATTCTTG AAAGAGAATA CAATAACCTC TTGGCCCTGG 
     4561 GAACTGAAAG GCGACTGGAT GAAAGTGATG ACAAGAACTT CAGACGTGGA TCAACCTGGA 
     4621 GAAGGCAGTT TCCTCCTCGT GAAGTACATG GAATCAGCAT GATGCCTGGG TCCTCAGAAA 
     4681 CATTACCAGC TGGATTTAGG TTAACCACAA CCTCTGGGCA GTCAAGAAAA ATGACAACAG 
     4741 ATGATGGCGT CTTTTCAGTC TACTCTACCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//