LOCUS       RC235223                8526 bp    DNA     circular  SYN 
DEFINITION  AP3D1 (Myc-DDK tagged) - Homo sapiens adaptor-related protein complex 3, delta 1 subunit (AP3D1), transcript variant 3
ACCESSION   NM_001261826
VERSION     NM_001261826.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4683..4774 
                     /label="Myc-DDK"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    4861..4880 
                     /label="Restriction Enzyme"  
     misc_feature    5665..6337 
                     /label="ColE1"  
     misc_feature    6767..7561 
                     /label="Kan/Neo"  
     misc_feature    7596..7898 
                     /label="SV40 ori"  
     misc_feature    7984..8461 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4665..4671 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4665 
                     /label="AP3D1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCAAG ATGGTGAAGG GCAGCATCGA CCGCATGTTC GACAAGAATC 
     1081 TGCAGGACTT GGTCCGCGGC ATCCGTAACC ACAAGGAGGA CGAGGCAAAA TACATATCTC 
     1141 AGTGCATTGA TGAGATCAAG CAGGAGCTGA AGCAGGACAA CATAGCGGTG AAGGCGAACG 
     1201 CGGTCTGCAA GCTGACGTAT TTACAGATGT TGGGATACGA CATCAGCTGG GCCGCCTTCA 
     1261 ACATCATAGA AGTGATGAGT GCCTCCAAGT TCACCTTCAA GCGAATTGGC TACCTCGCTG 
     1321 CTTCCCAGAG CTTTCACGAA GGCACCGACG TCATCATGCT GACCACCAAT CAGATCCGTA 
     1381 AGGACTTGAG CAGCCCCAGC CAGTACGACA CAGGTGTTGC ACTGACGGGT CTGTCCTGCT 
     1441 TCGTCACCCC AGACCTTGCC AGAGACCTGG CAAATGACAT CATGACACTG ATGTCACACA 
     1501 CCAAGCCCTA CATCAGGAAG AAGGCTGTGC TGATCATGTA CAAGGTGTTC CTGAAGTACC 
     1561 CCGAGTCGCT GCGCCCTGCC TTTCCCCGGC TGAAGGAGAA GCTGGAGGAC CCCGACCCCG 
     1621 GGGTTCAGTC GGCTGCCGTC AATGTCATCT GCGAGCTGGC CAGACGCAAC CCTAAGAACT 
     1681 ACCTGTCCCT GGCCCCGCTC TTTTTCAAGC TGATGACGTC CTCCACCAAC AACTGGGTCC 
     1741 TCATCAAGAT CATCAAGCTG TTCGGTGCTC TTACTCCTTT GGAACCGCGG CTGGGCAAGA 
     1801 AGCTGATCGA GCCCCTCACC AATCTCATCC ACAGCACGTC TGCCATGTCT CTCCTCTATG 
     1861 AATGTGTGAA CACCGTGATT GCAGTGCTCA TCTCGCTGTC CTCCGGCATG CCCAACCACA 
     1921 GCGCCAGCAT CCAGCTTTGT GTTCAGAAAT TAAGGATATT GATCGAGGAC TCCGATCAGA 
     1981 ACTTGAAGTA CCTGGGGCTG CTGGCAATGT CCAAGATCCT GAAGACCCAC CCCAAGTCCG 
     2041 TGCAGTCCCA CAAGGACCTC ATCCTGCAGT GCCTGGACGA CAAGGACGAG TCCATCCGGC 
     2101 TGCGGGCCCT GGACCTGCTC TATGGGATGG TGTCCAAGAA GAACCTGATG GAGATCGTGA 
     2161 AGAAGCTGAT GACCCACGTA GACAAGGCAG AGGGTACCAC CTACCGTGAC GAGCTGCTCA 
     2221 CCAAGATCAT TGACATCTGC AGCCAGTCCA ACTACCAGTA CATCACCAAC TTCGAGTGGT 
     2281 ACATCAGCAT CCTGGTGGAG CTGACCCGGC TGGAGGGCAC ACGGCACGGC CACCTCATCG 
     2341 CCGCCCAAAT GCTGGACGTG GCCATCCGCG TGAAGGCCAT CCGCAAGTTC GCCGTGTCCC 
     2401 AGATGTCTGC GCTGCTTGAC AGTGCACACC TGCTGGCCAG CAGCACCCAG CGGAACGGGA 
     2461 TCTGTGAGGT GCTGTACGCT GCCGCCTGGA TCTGCGGGGA GTTCTCAGAG CATCTGCAGG 
     2521 AACCACACCA CACTTTGGAG GCCATGCTGC GGCCCAGAGT CACCACGCTG CCAGGCCACA 
     2581 TCCAGGCCGT GTATGTGCAG AACGTGGTCA AGCTCTACGC CTCCATCCTG CAGCAGAAGG 
     2641 AGCAGGCCGG GGAGGCAGAG GGCGCTCAGG CCGTCACCCA GCTCATGGTG GACCGGCTGC 
     2701 CCCAGTTTGT GCAGAGCGCA GACCTGGAGG TGCAGGAGCG GGCGTCCTGC ATCCTGCAGC 
     2761 TGGTCAAGCA CATCCAGAAG CTTCAGGCCA AGGACGTGCC TGTGGCAGAG GAGGTCAGCG 
     2821 CTCTCTTTGC TGGGGAGCTG AACCCAGTGG CCCCCAAGGC CCAGAAGAAG GTTCCAGTCC 
     2881 CCGAAGGCCT GGACCTGGAC GCCTGGATCA ATGAGCCACT CTCGGACAGC GAGTCAGAGG 
     2941 ACGAGAGGCC CAGGGCCGTC TTCCACGAGG AGGAGCAGCG GCGTCCCAAG CACCGGCCGT 
     3001 CGGAGGCGGA CGAGGAAGAG CTGGCTCGGC GCCGAGAGGC CCGGAAGCAG GAGCAGGCCA 
     3061 ACAACCCCTT CTACATCAAG AGCTCGCCAT CGCCACAGAA GCGGTACCAG GACACCCCGG 
     3121 GCGTGGAGCA CATTCCCGTG GTGCAGATTG ACCTCTCCGT CCCCTTGAAG GTTCCAGGGC 
     3181 TGCCTATGTC AGATCAGTAT GTGAAGCTGG AGGAGGAGCG GCGGCACCGG CAGAAGCTGG 
     3241 AGAAGGACAA GAGGAGGAAA AAGAGGAAGG AGAAGGAGAA GAAGGGCAAG CGCCGCCACA 
     3301 GCTCGCTGCC CACGGAGAGC GACGAGGACA TCGCCCCTGC CCAGCAGGTG GACATCGTCA 
     3361 CAGAGGAGAT GCCTGAGAAT GCTCTGCCCA GCGACGAGGA TGACAAAGAC CCCAACGACC 
     3421 CCTACAGGGC TCTGGATATT GACCTGGATA AGCCCTTAGC CGACAGCGAG AAACTGCCTA 
     3481 TTCAGAAACA CAGAAACACC GAGACCTCAA AATCCCCTGA GAAGGACGTT CCCATGGTAG 
     3541 AAAAGAAGAG CAAGAAACCC AAGAAGAAAG AGAAAAAACA CAAAGAGAAA GAGAGAGACA 
     3601 AGGAGAAGAA GAAGGAGAAG GAGAAGAAGG CTGAGGACCT GGACTTCTGG CTGTCTACCA 
     3661 CCCCACCGCC TGCCCCCGCC CCCGCCCCCG CCCCCGTTCC ATCCACGGGG GAGCTCAGTG 
     3721 TGAACACTGT CACTACCCCG AAGGACGAGT GTGAGGACGC CAAGACGGAG GCGCAGGGCG 
     3781 AGGAGGACGA TGCCGAGGGG CAAGACCAGG ACAAGAAATC TCCCAAGCCT AAGAAGAAGA 
     3841 AGCACAGGAA GGAGAAGGAG GAGCGGACCA AAGGCAAGAA GAAGTCCAAG AAGCAGCCTC 
     3901 CAGGCAGCGA GGAGGCAGCG GGGGAGCCGG TGCAGAATGG CGCGCCAGAG GAGGAGCAGC 
     3961 TCCCGCCTGA GTCCAGCTAC TCCCTCCTCG CTGAAAATTC CTATGTTAAA ATGACCTGTG 
     4021 ACATCCGGGG CAGTCTGCAG GAGGACAGCC AGGTCACTGT GGCCATCGTG CTGGAGAACA 
     4081 GGAGCAGCAG CATCCTCAAG GGCATGGAGC TCAGCGTGCT GGACTCACTC AATGCCAGGA 
     4141 TGGCCCGGCC GCAGGGCTCC TCCGTCCACG ATGGCGTCCC CGTGCCTTTC CAGCTGCCCC 
     4201 CAGGCGTCTC CAACGAAGCC CAGTATGTGT TCACCATCCA GAGCATCGTC ATGGCGCAGA 
     4261 AGCTCAAGGG GACCCTGTCC TTCATTGCCA AGAATGACGA GGGTGCGACC CACGAGAAGC 
     4321 TGGACTTCAG GCTGCACTTC AGCTGCAGCT CCTACTTGAT CACCACTCCC TGCTACAGTG 
     4381 ACGCCTTTGC TAAGTTGCTG GAGTCTGGGG ACTTGAGCAT GAGCTCAATC AAAGTCGATG 
     4441 GCATTCGGAT GTCCTTCCAG AATCTTCTGG CGAAGATCTG TTTTCACCAC CATTTTTCCG 
     4501 TTGTGGAGCG AGTGGACTCC TGCGCCTCCA TGTACAGCCG CTCCATCCAG GGCCACCATG 
     4561 TCTGCCTCCT GGTGAAAAAG GGTGAGAACT CTGTCTCAGT CGACGGGAAG TGCAGTGACT 
     4621 CCACGCTACT GAGCAACTTG TTAGAAGAGA TGAAGGCGAC GCTGGCCAAG TGTACGCGTA 
     4681 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4741 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4801 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4861 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4921 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4981 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5041 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5101 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5161 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5221 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5281 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5341 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5401 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5461 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5521 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5581 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5641 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5701 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5761 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5821 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5881 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5941 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6001 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6061 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6121 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6181 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6241 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6301 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6361 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6421 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6481 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6541 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6601 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6661 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6721 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6781 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6841 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6901 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6961 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7021 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7081 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7141 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7201 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7261 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7321 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7381 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7441 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7501 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7561 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7621 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7681 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7741 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7981 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8041 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8101 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8161 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8221 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8281 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8341 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8401 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8461 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8521 AATTTT
//