LOCUS       RC235211                8469 bp    DNA     circular  SYN 
DEFINITION  SLC12A2 (Myc-DDK tagged) - Homo sapiens solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant 2
ACCESSION   NM_001256461
VERSION     NM_001256461.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4626..4717 
                     /label="Myc-DDK"  
     misc_feature    4747..5332 
                     /label="PolyA signal"  
     misc_feature    4804..4823 
                     /label="Restriction Enzyme"  
     misc_feature    5608..6280 
                     /label="ColE1"  
     misc_feature    6710..7504 
                     /label="Kan/Neo"  
     misc_feature    7539..7841 
                     /label="SV40 ori"  
     misc_feature    7927..8404 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4608..4614 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4608 
                     /label="SLC12A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCGG CCCACGGCGC CCTCCTCCGG CGCCCCGGGA CTGGCCGGGG 
     1081 TCGGGGAGAC GCCGTCAGCC GCTGCGCTGG CCGCAGCCAG GGTGGAACTG CCCGGCACGG 
     1141 CTGTGCCCTC GGTGCCGGAG GATGCTGCGC CCGCGAGCCG GGACGGCGGC GGGGTCCGCG 
     1201 ATGAGGGCCC CGCGGCGGCC GGGGACGGGC TGGGCAGACC CTTGGGGCCC ACCCCGAGCC 
     1261 AGAGCCGTTT CCAGGTGGAC CTGGTTTCCG AGAACGCCGG GCGGGCCGCT GCTGCGGCGG 
     1321 CGGCGGCGGC GGCGGCAGCG GCGGCGGCTG GTGCTGGGGC GGGGGCCAAG CAGACCCCCG 
     1381 CGGACGGGGA AGCCAGCGGC GAGAGCGAGC CGGCTAAAGG CAGCGAGGAA GCCAAGGGCC 
     1441 GCTTCCGCGT GAACTTCGTG GACCCAGCTG CCTCCTCGTC GGCTGAAGAC AGCCTGTCAG 
     1501 ATGCTGCCGG GGTCGGAGTC GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG 
     1561 TGCTGAGCGA GGGCAGCAGC CTGCACTCCG GCGGCGGCGG CGGCAGTGGG CACCACCAGC 
     1621 ACTACTATTA TGATACCCAC ACCAACACCT ACTACCTGCG CACCTTCGGC CACAACACCA 
     1681 TGGACGCTGT GCCCAGGATC GATCACTACC GGCACACAGC CGCGCAGCTG GGCGAGAAGC 
     1741 TGCTCCGGCC TAGCCTGGCG GAGCTCCACG ACGAGCTGGA AAAGGAACCT TTTGAGGATG 
     1801 GCTTTGCAAA TGGGGAAGAA AGTACTCCAA CCAGAGATGC TGTGGTCACG TATACTGCAG 
     1861 AAAGTAAAGG AGTCGTGAAG TTTGGCTGGA TCAAGGGTGT ATTAGTACGT TGTATGTTAA 
     1921 ACATTTGGGG TGTGATGCTT TTCATTAGAT TGTCATGGAT TGTGGGTCAA GCTGGAATAG 
     1981 GTCTATCAGT CCTTGTAATA ATGATGGCCA CTGTTGTGAC AACTATCACA GGATTGTCTA 
     2041 CTTCAGCAAT AGCAACTAAT GGATTTGTAA GAGGAGGAGG AGCATATTAT TTAATATCTA 
     2101 GAAGTCTAGG GCCAGAATTT GGTGGTGCAA TTGGTCTAAT CTTCGCCTTT GCCAACGCTG 
     2161 TTGCAGTTGC TATGTATGTG GTTGGATTTG CAGAAACCGT GGTGGAGTTG CTTAAGGAAC 
     2221 ATTCCATACT TATGATAGAT GAAATCAATG ATATCCGAAT TATTGGAGCC ATTACAGTCG 
     2281 TGATTCTTTT AGGTATCTCA GTAGCTGGAA TGGAGTGGGA AGCAAAAGCT CAGATTGTTC 
     2341 TTTTGGTGAT CCTACTTCTT GCTATTGGTG ATTTCGTCAT AGGAACATTT ATCCCACTGG 
     2401 AGAGCAAGAA GCCAAAAGGG TTTTTTGGTT ATAAATCTGA AATATTTAAT GAGAACTTTG 
     2461 GGCCCGATTT TCGAGAGGAA GAGACTTTCT TTTCTGTATT TGCCATCTTT TTTCCTGCTG 
     2521 CAACTGGTAT TCTGGCTGGA GCAAATATCT CAGGTGATCT TGCAGATCCT CAGTCAGCCA 
     2581 TACCCAAAGG AACACTCCTA GCCATTTTAA TTACTACATT GGTTTACGTA GGAATTGCAG 
     2641 TATCTGTAGG TTCTTGTGTT GTTCGAGATG CCACTGGAAA CGTTAATGAC ACTATCGTAA 
     2701 CAGAGCTAAC AAACTGTACT TCTGCAGCCT GCAAATTAAA CTTTGATTTT TCATCTTGTG 
     2761 AAAGCAGTCC TTGTTCCTAT GGCCTAATGA ACAACTTCCA GGTAATGAGT ATGGTGTCAG 
     2821 GATTTACACC ACTAATTTCT GCAGGTATAT TTTCAGCCAC TCTTTCTTCA GCATTAGCAT 
     2881 CCCTAGTGAG TGCTCCCAAA ATATTTCAGG CTCTATGTAA GGACAACATC TACCCAGCTT 
     2941 TCCAGATGTT TGCTAAAGGT TATGGGAAAA ATAATGAACC TCTTCGTGGC TACATCTTAA 
     3001 CATTCTTAAT TGCACTTGGA TTCATCTTAA TTGCTGAACT GAATGTTATT GCACCAATTA 
     3061 TCTCAAACTT CTTCCTTGCA TCATATGCAT TGATCAATTT TTCAGTATTC CATGCATCAC 
     3121 TTGCAAAATC TCCAGGATGG CGTCCTGCAT TCAAATACTA CAACATGTGG ATATCACTTC 
     3181 TTGGAGCAAT TCTTTGTTGC ATAGTAATGT TCGTCATTAA CTGGTGGGCT GCATTGCTAA 
     3241 CATATGTGAT AGTCCTTGGG CTGTATATTT ATGTTACCTA CAAAAAACCA GATGTGAATT 
     3301 GGGGATCCTC TACACAAGCC CTGACTTACC TGAATGCACT GCAGCATTCA ATTCGTCTTT 
     3361 CTGGAGTGGA AGACCACGTG AAAAACTTTA GGCCACAGTG TCTTGTTATG ACAGGTGCTC 
     3421 CAAACTCACG TCCAGCTTTA CTTCATCTTG TTCATGATTT CACAAAAAAT GTTGGTTTGA 
     3481 TGATCTGTGG CCATGTACAT ATGGGTCCTC GAAGACAAGC CATGAAAGAG ATGTCCATCG 
     3541 ATCAAGCCAA ATATCAGCGA TGGCTTATTA AGAACAAAAT GAAGGCATTT TATGCTCCAG 
     3601 TACATGCAGA TGACTTGAGA GAAGGTGCAC AGTATTTGAT GCAGGCTGCT GGTCTTGGTC 
     3661 GTATGAAGCC AAACACACTT GTCCTTGGAT TTAAGAAAGA TTGGTTGCAA GCAGATATGA 
     3721 GGGATGTGGA TATGTATATA AACTTATTTC ATGATGCTTT TGACATACAA TATGGAGTAG 
     3781 TGGTTATTCG CCTAAAAGAA GGTCTGGATA TATCTCATCT TCAAGGACAA GAAGAATTAT 
     3841 TGTCATCACA AGAGAAATCT CCTGGCACCA AGGATGTGGT AGTAAGTGTG GAATATAGTA 
     3901 AAAAGTCCGA TTTAGATACT TCCAAACCAC TCAGTGAAAA ACCAATTACA CACAAAGAAT 
     3961 CCAAAGGCCC TATTGTGCCT TTAAATGTAG CTGACCAAAA GCTTCTTGAA GCTAGTACAC 
     4021 AGTTTCAGAA AAAACAAGGA AAGAATACTA TTGATGTCTG GTGGCTTTTT GATGATGGAG 
     4081 GTTTGACCTT ATTGATACCT TACCTTCTGA CGACCAAGAA AAAATGGAAA GACTGTAAGA 
     4141 TCAGAGTATT CATTGGTGGA AAGATAAACA GAATAGACCA TGACCGGAGA GCGATGGCTA 
     4201 CTTTGCTTAG CAAGTTCCGG ATAGACTTTT CTGATATCAT GGTTCTAGGA GATATCAATA 
     4261 CCAAACCAAA GAAAGAAAAT ATTATAGCTT TTGAGGAAAT CATTGAGCCA TACAGACTTC 
     4321 ATGAAGATGA TAAAGAGCAA GATATTGCAG ATAAAATGAA AGAAGATGAA CCATGGCGAA 
     4381 TAACAGATAA TGAGCTTGAA CTTTATAAGA CCAAGACATA CCGGCAGATC AGGTTAAATG 
     4441 AGTTATTAAA GGAACATTCA AGCACAGCTA ATATTATTGT CATGAGTCTC CCAGTTGCAC 
     4501 GAAAAGGTGC TGTGTCTAGT GCTCTCTACA TGGCATGGTT AGAAGCTCTA TCTAAGGACC 
     4561 TACCACCAAT CCTCCTAGTT CGTGGGAATC ATCAGAGTGT CCTTACCTTC TATTCAACGC 
     4621 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4681 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5521 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6241 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6301 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6361 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6421 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6481 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6541 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6601 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6661 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6721 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6781 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6841 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6901 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6961 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7021 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7081 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7141 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7201 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7261 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7321 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7381 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7441 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7501 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7561 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7621 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7681 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7921 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7981 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8041 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8101 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8161 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8221 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8281 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8341 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8401 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8461 GCGAATTTT
//