LOCUS       RC235184                8301 bp    DNA     circular  SYN 
DEFINITION  MEGF10 (Myc-DDK tagged) - Homo sapiens multiple EGF-like-domains 10 (MEGF10), transcript variant 2
ACCESSION   NM_001256545
VERSION     NM_001256545.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4458..4549 
                     /label="Myc-DDK"  
     misc_feature    4579..5164 
                     /label="PolyA signal"  
     misc_feature    4636..4655 
                     /label="Restriction Enzyme"  
     misc_feature    5440..6112 
                     /label="ColE1"  
     misc_feature    6542..7336 
                     /label="Kan/Neo"  
     misc_feature    7371..7673 
                     /label="SV40 ori"  
     misc_feature    7759..8236 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4440..4446 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4440 
                     /label="MEGF10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTATTTCT TTGAACTCAT GCCTGAGCTT TATTTGTTTA TTGTTATGCC 
     1081 ACTGGATTGG GACAGCATCA CCTCTGAATC TTGAAGACCC TAATGTGTGT AGCCACTGGG 
     1141 AAAGCTACTC AGTGACTGTG CAAGAGTCAT ACCCACATCC CTTTGATCAA ATTTACTACA 
     1201 CGAGCTGCAC TGACATTCTA AACTGGTTTA AATGCACGCG GCACAGAGTC AGCTATCGGA 
     1261 CAGCCTATCG ACATGGGGAG AAGACTATGT ATAGGCGCAA GTCTCAGTGT TGTCCTGGAT 
     1321 TTTATGAAAG CGGGGAAATG TGTGTCCCCC ACTGTGCTGA TAAATGTGTC CATGGTCGCT 
     1381 GTATTGCTCC AAACACCTGT CAGTGTGAGC CTGGCTGGGG AGGGACCAAC TGCTCCAGTG 
     1441 CCTGCGATGG TGATCACTGG GGTCCCCACT GCACCAGCCG GTGCCAGTGC AAAAATGGGG 
     1501 CTCTGTGCAA CCCCATCACC GGGGCTTGCC ACTGTGCTGC GGGCTTCCGG GGCTGGCGCT 
     1561 GCGAGGACCG CTGTGAGCAG GGCACCTATG GTAACGACTG TCATCAGAGA TGCCAGTGCC 
     1621 AGAATGGAGC CACCTGCGAC CACGTCACGG GGGAATGCCG CTGCCCACCA GGATACACCG 
     1681 GAGCCTTCTG TGAGGATCTT TGTCCTCCTG GTAAACATGG TCCACAGTGT GAGCAGAGAT 
     1741 GCCCTTGTCA AAATGGAGGA GTGTGTCATC ACGTCACTGG AGAATGCTCT TGCCCTTCTG 
     1801 GCTGGATGGG CACAGTGTGT GGTCAGCCTT GCCCCGAGGG TCGCTTTGGA AAGAACTGTT 
     1861 CCCAAGAATG CCAGTGCCAT AATGGAGGGA CGTGTGATGC TGCCACAGGC CAATGTCATT 
     1921 GCAGTCCAGG ATACACAGGG GAACGGTGCC AGGATGAGTG TCCTGTTGGG ACCTATGGCG 
     1981 TTCTCTGTGC TGAGACCTGC CAGTGTGTCA ACGGAGGGAA GTGTTACCAC GTGAGCGGCG 
     2041 CATGCCTCTG TGAAGCAGGC TTTGCTGGCG AGCGCTGCGA AGCACGCCTG TGTCCTGAGG 
     2101 GGCTCTACGG CATCAAATGT GACAAACGGT GTCCCTGCCA CCTGGAAAAC ACTCATAGCT 
     2161 GTCACCCCAT GTCTGGAGAG TGTGCCTGCA AGCCGGGCTG GTCAGGACTC TACTGTAATG 
     2221 AGACATGTTC TCCTGGATTC TACGGGGAAG CTTGCCAGCA GATCTGCAGC TGCCAAAATG 
     2281 GGGCAGACTG TGACAGTGTG ACTGGAAAGT GCACCTGTGC CCCAGGATTC AAAGGAATTG 
     2341 ACTGCTCTAC CCCATGCCCT CTGGGAACCT ATGGGATAAA CTGTTCCTCT CGCTGTGGCT 
     2401 GTAAAAATGA TGCAGTCTGC TCTCCTGTGG ACGGGTCTTG TACTTGCAAG GCAGGCTGGC 
     2461 ACGGGGTGGA CTGCTCCATC AGATGTCCCA GTGGCACATG GGGCTTTGGC TGTAACTTAA 
     2521 CATGCCAGTG CCTCAACGGG GGAGCCTGCA ACACCCTGGA CGGGACCTGC ACGTGTGCAC 
     2581 CTGGATGGCG CGGGGAGAAA TGCGAACTTC CCTGCCAGGA TGGCACGTAC GGGCTGAACT 
     2641 GTGCTGAGCG CTGCGACTGC AGCCACGCAG ATGGCTGCCA CCCTACCACG GGCCATTGCC 
     2701 GCTGCCTCCC CGGATGGTCA GGTGTCCACT GTGACAGCGT GTGTGCTGAG GGACGCTGGG 
     2761 GCCCCAACTG CTCCCTGCCC TGCTACTGTA AAAATGGGGC TTCATGCTCC CCTGATGATG 
     2821 GCATCTGCGA GTGTGCACCA GGCTTCCGAG GCACCACTTG TCAGAGGATC TGCTCCCCTG 
     2881 GTTTTTATGG GCATCGCTGC AGCCAGACAT GCCCACAGTG CGTTCACAGC AGCGGGCCCT 
     2941 GCCACCACAT CACCGGCCTG TGTGACTGCT TGCCTGGCTT CACAGGCGCC CTCTGCAATG 
     3001 AAGTGTGTCC CAGTGGCAGA TTTGGGAAAA ACTGTGCAGG AATTTGTACC TGCACCAACA 
     3061 ACGGAACCTG TAACCCCATT GACAGATCTT GTCAGTGTTA CCCCGGTTGG ATTGGCAGTG 
     3121 ACTGCTCTCA ACCATGTCCA CCTGCCCACT GGGGCCCAAA CTGCATCCAC ACGTGCAACT 
     3181 GCCATAATGG AGCTTTCTGC AGCGCCTACG ATGGGGAATG TAAATGCACT CCTGGCTGGA 
     3241 CAGGGCTCTA CTGCACTCAG AGATGTCCTC TAGGGTTTTA TGGAAAAGAT TGTGCACTGA 
     3301 TATGCCAATG TCAAAACGGA GCTGACTGCG ACCACATTTC TGGGCAGTGT ACTTGCCGCA 
     3361 CTGGATTCAT GGGACGGCAC TGTGAGCAGA AGTGCCCTTC AGGAACATAT GGCTATGGCT 
     3421 GTCGCCAGAT ATGTGATTGT CTGAACAACT CCACCTGCGA CCACATCACT GGGACCTGTT 
     3481 ACTGCAGCCC CGGATGGAAG GGAGCGAGAT GTGATCAAGC TGGTGTTATC ATAGTTGGAA 
     3541 ATCTGAACAG CTTAAGCCGA ACCAGTACTG CTCTCCCTGC TGATTCCTAC CAGATCGGGG 
     3601 CCATTGCAGG CATCATCATT CTTGTCCTAG TTGTTCTCTT CCTACTGGCA TTGTTCATTA 
     3661 TTTATAGACA CAAGCAGAAG GGAAAGGAAT CAAGCATGCC AGCAGTTACC TACACCCCTG 
     3721 CTATGAGGGT CGTCAATGCA GATTATACCA TTTCAGGAAC CCTTCCTCAC AGCAATGGTG 
     3781 GAAACGCTAA TAGCCACTAC TTCACCAATC CCAGTTACCA CACGCTCACC CAGTGTGCCA 
     3841 CATCCCCTCA CGTCAACAAC AGGGACAGGA TGACTGTCAC GAAGTCAAAA AACAATCAAC 
     3901 TGTTTGTGAA TCTTAAAAAT GTGAACCCTG GGAAGAGAGG CCCTGTGGGG GACTGCACTG 
     3961 GGACATTGCC GGCTGACTGG AAACATGGCG GCTACCTCAA CGAGCTCGGT GCTTTTGGAC 
     4021 TTGACAGAAG CTATATGGGA AAATCCTTAA AAGACCTGGG AAAGAATTCT GAATATAATT 
     4081 CAAGTAACTG CTCCCTAAGC AGTTCTGAGA ACCCATATGC CACTATTAAA GACCCACCTG 
     4141 TACTTATCCC GAAAAGCTCA GAGTGTGGTT ATGTGGAGAT GAAATCGCCG GCACGAAGAG 
     4201 ATTCCCCATA TGCAGAGATC AATAACTCAA CTTCAGCCAA CAGGAATGTC TATGAAGTTG 
     4261 AACCTACAGT GAGTGTTGTC CAAGGAGTAT TCAGCAATAA TGGGCGTCTC TCCCAGGATC 
     4321 CATATGACCT CCCAAAGAAC AGTCACATCC CTTGTCATTA TGACCTGCTG CCAGTCCGAG 
     4381 ACAGTTCATC CTCCCCTAAG CAAGAGGACA GTGGTGGTAG CAGCAGCAAC AGCAGCAGCA 
     4441 GCAGTGAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4501 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4561 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4621 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4681 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4741 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4801 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4861 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4921 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4981 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5041 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5101 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5161 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5221 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5281 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5341 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5401 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5461 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5521 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5581 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5641 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5701 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5761 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5821 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5881 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5941 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6001 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6061 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6121 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6181 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6241 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6301 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6361 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6421 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6481 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6541 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6601 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6661 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6721 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6781 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6841 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6901 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6961 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7021 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7081 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7141 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7201 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7261 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7321 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7381 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7441 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7501 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7561 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7621 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7741 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7801 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7861 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7921 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7981 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8041 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8101 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8161 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8221 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8281 CAAAAATTTA ACGCGAATTT T
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