LOCUS       RC235171                8217 bp    DNA     circular  SYN 
DEFINITION  DIAPH3 (Myc-DDK tagged) - Homo sapiens diaphanous-related formin 3 (DIAPH3), transcript variant 6
ACCESSION   NM_001258369
VERSION     NM_001258369.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4374..4465 
                     /label="Myc-DDK"  
     misc_feature    4495..5080 
                     /label="PolyA signal"  
     misc_feature    4552..4571 
                     /label="Restriction Enzyme"  
     misc_feature    5356..6028 
                     /label="ColE1"  
     misc_feature    6458..7252 
                     /label="Kan/Neo"  
     misc_feature    7287..7589 
                     /label="SV40 ori"  
     misc_feature    7675..8152 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4356..4362 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4356 
                     /label="DIAPH3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACGGCAC CAGCCGCGGC TGCACCACCC GGCCCAAGGC TCAGCCGCTG 
     1081 GGACTCCCTA CCCTTCCTCA GCCTCTCTCC GCGGCTGCCG GGAAAGCAAG ATGCCGCGCA 
     1141 GGAAGGGCCC CCAACACCCT CCGCCGCCCA GTGGCCCCGA GGAGCCTGGG GAGAAGCGCC 
     1201 CCAAGTTTCA TTTAAATATT AGGACACTGA CGGATGATAT GCTGGACAAA TTTGCCAGCA 
     1261 TAAGAATTCC AGGGAGCAAG AAAGAGAGAC CTCCACTTCC CAACCTGAAG ACTGCATTTG 
     1321 CAAGCAGTGA TTGCTCAGCA GCACCTTTAG AGATGATGGA GAACTTTCCA AAGCCACTGT 
     1381 CAGAGAATGA ACTCTTAGAA CTTTTTGAAA AAATGATGGA AGATATGAAT TTAAATGAAG 
     1441 ATAAAAAGGC ACCATTGCGG GAAAAGGACT TCAGTATCAA AAAAGAAATG GTGATGCAGT 
     1501 ACATTAATAC TGCTTCTAAG ACAGGAAGTC TTAAGAGAAG CCGACAGATC TCACCTCAGG 
     1561 AATTCATTCA TGAGCTGAAA ATGGGGTCTG CAGATGAGAG ACTTGTCACA TGCCTGGAGT 
     1621 CTCTCCGAGT GTCTTTGACC AGCAATCCTG TGAGTTGGGT GGAAAGCTTT GGACATGAAG 
     1681 GGCTTGGATT ATTATTAGAC ATTTTGGAAA AACTGATTAG TGGAAAAATC CAAGAAAAAG 
     1741 TTGTAAAGAA AAATCAACAT AAAGTCATAC AGTGTCTAAA AGCCCTGATG AATACGCAGT 
     1801 ATGGCTTGGA AAGAATTATG AGTGAGGAGA GGAGCCTTTC CTTATTGGCC AAAGCCGTGG 
     1861 ATCCCAGACA CCCCAATATG ATGACAGATG TGGTTAAACT TCTCTCTGCG GTATGCATTG 
     1921 TAGGGGAAGA AAGCATCCTT GAAGAAGTTT TAGAAGCTTT AACTTCAGCT GGTGAAGAAA 
     1981 AAAAAATTGA CAGATTTTTT TGTATTGTGG AAGGCCTCCG GCACAATTCA GTTCAACTGC 
     2041 AAGTAGCTTG TATGCAGCTC ATCAATGCCC TGGTTACATC TCCTGATGAT TTGGATTTCA 
     2101 GGCTTCACAT CAGAAATGAA TTTATGCGTT GTGGATTGAA AGAGATATTG CCAAATTTAA 
     2161 AATGCATTAA GAATGATGGC CTGGATATCC AACTTAAAGT CTTTGATGAG CATAAAGAAG 
     2221 AAGATTTGTT TGAGTTATCC CATCGCCTTG AAGATATTAG AGCTGAACTT GATGAAGCAT 
     2281 ATGATGTTTA CAACATGGTG TGGAGCACAG TTAAAGAAAC TAGAGCAGAG GGATATTTTA 
     2341 TTTCTATTCT TCAGCATCTT TTGCTGATTC GAAATGATTA TTTTATAAGG CAACAATACT 
     2401 TCAAATTAAT TGATGAGTGT GTATCCCAGA TTGTATTGCA TAGAGATGGA ATGGATCCAG 
     2461 ACTTCACATA TCGAAAAAGA CTAGATTTAG ATTTAACCCA GTTTGTAGAC ATTTGCATAG 
     2521 ATCAAGCAAA ACTAGAAGAG TTTGAAGAGA AAGCATCAGA ACTTTACAAG AAATTTGAAA 
     2581 AAGAGTTTAC CGACCACCAA GAAACTCAGG CTGAATTGCA GAAAAAAGAG GCAAAGATTA 
     2641 ATGAGCTTCA AGCAGAGCTA CAAGCTTTTA AGTCTCAGTT TGGTGCCTTG CCAGCTGATT 
     2701 GTAATATTCC TTTGCCTCCC TCTAAAGAAG GTGGAACTGG CCACTCAGCA CTTCCTCCTC 
     2761 CGCCTCCACT GCCTTCTGGT GGAGGGGTGC CGCCTCCACC TCCTCCCCCA CCACCTCCTC 
     2821 CACTTCCAGG AATGCGGATG CCATTCAGTG GTCCTGTGCC TCCACCACCT CCCCTGGGAT 
     2881 TCCTTGGAGG ACAAAATTCT CCTCCTCTAC CAATCCTGCC ATTTGGGTTG AAACCAAAGA 
     2941 AAGAATTTAA ACCTGAAATC AGCATGAGAA GATTGAATTG GTTAAAGATC AGACCTCATG 
     3001 AAATGACTGA AAACTGTTTC TGGATAAAAG TAAATGAAAA TAAGTATGAA AACGTGGATT 
     3061 TGCTTTGTAA ACTTGAGAAT ACATTTTGTT GCCAACAAAA AGAGAGAAGA GAAGAGGAAG 
     3121 ATATTGAAGA GAAGAAATCG ATTAAGAAAA AAATTAAAGA ACTTAAGTTT TTAGATTCTA 
     3181 AAATTGCCCA GAACCTTTCA ATCTTCCTGA GCTCTTTTCG GGTGCCATAT GAGGAAATCA 
     3241 GAATGATGAT ATTGGAAGTA GATGAAACAC GGTTGGCAGA GTCTATGATT CAGAACTTAA 
     3301 TAAAGCATCT TCCTGATCAA GAGCAATTAA ATTCATTGTC TCAGTTCAAG AGTGAATATA 
     3361 GCAACTTATG TGAACCTGAG CAGTTTGTGG TTGTGATGAG CAATGTGAAG AGACTACGGC 
     3421 CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC AACATCAAAC 
     3481 CTGACATCAT GGCTGTCAGT ACTGCCTGCG AAGAGATAAA GAAGAGCAAA AGCTTTAGCA 
     3541 AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC CGGAATGCTC 
     3601 AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACAAAA TCAGCAGATC 
     3661 AGAAAACAAC GCTACTTCAT TTCCTGGTAG AAATATGTGA AGAGAAGTAC CCTGATATAC 
     3721 TGAATTTTGT GGATGATTTG GAACCTTTAG ACAAAGCTAG TAAAGTCTCT GTAGAAACGC 
     3781 TGGAAAAGAA TTTGAGGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAG GAATTGGAAA 
     3841 CCTTTCCCCC TCCTGAGGAC TTGCATGACA AGTTTGTGAC AAAGATGTCC AGATTTGTTA 
     3901 TCAGTGCAAA AGAACAATAT GAGACACTTT CGAAGTTACA CGAAAACATG GAAAAGTTAT 
     3961 ACCAGAGTAT AATAGGATAC TATGCCATTG ATGTGAAGAA GGTGTCTGTG GAAGACTTTC 
     4021 TTACTGACCT GAATAACTTC AGAACCACAT TCATGCAAGC AATAAAGGAG AATATCAAAA 
     4081 AAAGAGAAGC AGAGGAAAAA GAAAAACGTG TCAGAATAGC TAAAGAATTA GCAGAGCGAG 
     4141 AAAGACTCGA ACGCCAACAA AAGAAAAAGC GTTTATTAGA AATGAAGACT GAGGGTGATG 
     4201 AGACAGGAGT GATGGATAAT CTGCTGGAGG CCTTGCAGTC CGGGGCTGCC TTCCGCGACA 
     4261 GAAGAAAAAG GACACCGATG CCAAAAGATG TTCGGCAGAG TCTCAGTCCA ATGTCTCAGA 
     4321 GGCCTGTTCT GAAAGTTTGT AACCATGGTA ATAAACCGTA TTTAACGCGT ACGCGGCCGC 
     4381 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4441 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4501 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4561 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4621 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4681 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4741 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4801 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4861 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4921 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4981 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5041 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5101 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5161 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5221 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5281 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5341 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5401 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5461 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5521 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5581 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5641 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5701 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5761 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5821 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5881 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5941 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6001 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6061 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6121 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6181 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6241 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6301 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6361 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6421 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6481 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6541 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6601 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6661 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6721 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6781 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6841 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6901 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6961 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7021 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7081 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7141 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7201 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7261 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7321 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7381 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7441 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7501 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7621 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7681 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7741 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7801 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7861 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7921 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7981 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8041 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8101 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8161 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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