LOCUS       RC235089                7791 bp    DNA     circular  SYN 
DEFINITION  DDX11 (Myc-DDK tagged) - Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (DDX11), transcript variant 4
ACCESSION   NM_001257144
VERSION     NM_001257144.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3948..4039 
                     /label="Myc-DDK"  
     misc_feature    4069..4654 
                     /label="PolyA signal"  
     misc_feature    4126..4145 
                     /label="Restriction Enzyme"  
     misc_feature    4930..5602 
                     /label="ColE1"  
     misc_feature    6032..6826 
                     /label="Kan/Neo"  
     misc_feature    6861..7163 
                     /label="SV40 ori"  
     misc_feature    7249..7726 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3930..3936 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3930 
                     /label="DDX11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAATGAA ACACAGAAGG TTGGTGCCAT CCATTTTCCT TTTCCCTTCA 
     1081 CACCCTATTC CATCCAGGAA GACTTCATGG CAGAGCTGTA CCGGGTTTTG GAGGCTGGCA 
     1141 AGATTGGGAT ATTTGAGAGT CCAACTGGCA CTGGGAAGTC CTTAAGTCTT ATTTGTGGGG 
     1201 CCCTCTCTTG GCTCCGTGAC TTTGAACAGA AGAAGCGTGA AGAAGAGGCA CGACTCCTTG 
     1261 AAACTGGAAC TGGCCCCTTA CATGATGAGA AAGATGAATC CCTGTGTCTG TCTTCTTCCT 
     1321 GCGAAGGGGC TGCAGGCACC CCGAGGCCTG CTGGAGAACC GGCCTGGGTT ACTCAGTTTG 
     1381 TGCAGAAGAA AGAAGAGAGG GACCTGGTGG ACCGACTAAA GGCGGAGCAG GCCAGGAGGA 
     1441 AGCAGCGAGA AGAACGCCTG CAGCAGCTGC AGCACAGGGT GCAGCTCAAG TATGCAGCCA 
     1501 AGCGCCTGAG GCAGGAAGAA GAAGAAAGAG AGAATCTCCT CCGCCTCAGC AGGGAGATGC 
     1561 TAGAGACAGG CCCGGAGGCT GAGCGGCTGG AGCAGCTGGA GTCTGGGGAG GAGGAGCTGG 
     1621 TCCTCGCCGA ATACGAGAGT GATGAGGAGA AAAAGGTGGC GAGCAGAGTG GATGAGGATG 
     1681 AGGATGACCT GGAGGAAGAA CACATAACTA AGATTTATTA CTGTAGTCGG ACACACTCCC 
     1741 AGCTGGCCCA GTTTGTGCAT GAGGTGAAGA AGAGCCCCTT TGGCAAGGAT GTTCGGCTGG 
     1801 TCTCCCTTGG CTCCCGGCAG AACCTTTGTG TAAATGAAGA CGTGAAAAGC CTAGGTTCTG 
     1861 TGCAGCTTAT CAACGACCGC TGTGTGGACA TGCAGAGAAG CAGGCACGAG AAGAAGAAAG 
     1921 GAGCTGAGGA GGAGAAGCCA AAGAGGAGGA GGCAGGAGAA GCAGGCAGCC TGCCCCTTCT 
     1981 ACAACCACGA GCAGATGGGC CTTCTCCGGG ATGAGGCCCT GGCAGAGGTG AAGGACATGG 
     2041 AGCAGCTGCT GGCCCTTGGG AAGGAGGCCC GGGCCTGTCC CTATTACGGG AGCCGCCTTG 
     2101 CCATCCCTGC AGCCCAGCTG GTGGTGCTGC CCTATCAGAT GCTGCTGCAT GCGGCCACTC 
     2161 GGCAGGCCGC GGGCATCCGG CTGCAGGACC AGGTGGTGAT CATCGACGAG GCGCACAACC 
     2221 TGATCGACAC CATCACGGGC ATGCACAGCG TGGAGGTCAG CGGCTCCCAG CTCTGCCAGG 
     2281 CCCATTCCCA GCTGCTGCAG TACGTGGAGC GATACGGGAA GCGTTTGAAG GCCAAGAACC 
     2341 TGATGTACCT GAAGCAGATC CTGTATTTGC TGGAGAAATT CGTGGCTGTG CTAGGGGGGA 
     2401 ACATTAAGCA AAATCCCAAT ACACAGAGTC TGTCACAGAC AGGGACGGAG CTGAAGACCA 
     2461 TCAACGACTT TCTCTTCCAG AGCCAGATCG ACAACATCAA CCTGTTCAAG GTGCAGCGAT 
     2521 ACTGTGAGAA GAGCATGATC AGCAGAAAGC TCTTTGGATT CACTGAACGG TACGGAGCAG 
     2581 TGTTCTCATC CCGGGAGCAG CCCAAACTGG CTGGGTTTCA GCAATTCCTG CAGAGCCTGC 
     2641 AGCCCAGGAC GACTGAAGCT CTTGCAGCCC CTGCAGACGA GAGTCAGGCC AGCACCCTGC 
     2701 GACCAGCTTC TCCACTGATG CACATCCAAG GCTTCCTGGC AGCTCTCACT ACGGCCAACC 
     2761 AGGACGGCAG GGTCATCCTG AGCCGCCAAG GCAGCCTCAG TCAGAGCACC CTGAAGTTTT 
     2821 TGCTCCTGAA TCCAGCTGTG CACTTTGCCC AAGTGGTGAA GGAATGCCGG GCAGTGGTCA 
     2881 TTGCGGGGGG TACCATGCAG CCGGTGTCTG ACTTCCGGCA GCAGCTGCTG GCCTGTGCCG 
     2941 GGGTGGAAGC TGAGCGCGTG GTGGAGTTTT CCTGTGGTCA CGTGATCCCT CCAGACAACA 
     3001 TCCTGCCCCT CGTCATCTGC AGCGGGATCT CCAACCAGCC GCTGGAATTC ACGTTCCAGA 
     3061 AAAGAGAGCT GCCTCAGATG ATGGACGAGG TGGGTCGCAT TCTCTGTAAC CTGTGCGGTG 
     3121 TGGTTCCTGG AGGGGTGGTC TGTTTCTTCC CCTCCTACGA GTACCTGCGC CAGGTCCATG 
     3181 CCCACTGGGA GAAGGGTGGC CTGCTGGGCC GTCTGGCTGC CAGGAAGAAG ATATTCCAGG 
     3241 AACCTAAGAG CGCACACCAG GTGGAGCAGG TGCTGCTGGC ATATTCCAGG TGCATCCAGG 
     3301 CCTGTGGCCA GGAGAGAGGC CAGGTGACAG GGGCCCTGCT CCTCTCTGTG GTTGGAGGAA 
     3361 AGATGAGTGA AGGGATCAAC TTCTCTGACA ACCTAGGCCG GTGTGTGGTG ATGGTGGGCA 
     3421 TGCCCTTCCC CAACATCAGG TCTGCAGAGC TGCAGGAGAA GATGGCCTAC TTGGATCAAA 
     3481 CCCTCAGCCC CCGGCCAGGC ACCCCCAGGG AAGGCTCTGG TGGAGAACCT GTGCATGAAG 
     3541 GCCGTCAACC AGTCCATAGG CAGGGCCATC AGGCACCAGA AGGATTTTGC CAGCGTAGTG 
     3601 CTCCTGGACC AGCGATATGC CCGGCCCCCT GTCCTGGCCA AGCTGCCGGC CTGGATCCGA 
     3661 GCCCGTGTGG AGGTCAAAGC TACCTTTGGC CCCGCCATTG CTGCTGTGCA GAAGTTTCAC 
     3721 CGGGAGAAGT CGGCCTCTTC CTGATGGGCA ACCACACCAC TGCCTGGCGC CGTGCCCTTC 
     3781 CTTTGTCCTG CCCGCTGGAG ACAGTGTTTG TCGTGGGCGT GGTCTGCGGG GATCCTGTTA 
     3841 CAAAGGTGAA ACCCAGGAGG AGAGTGTGGA GTCCAGAGTG CTGCCAGGAC CCAGGCACAG 
     3901 GCGTTAGCTC CCGTAGGAGA AAATGGGGGA ATCCTGAAAC GCGTACGCGG CCGCTCGAGC 
     3961 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4021 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4081 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4141 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4201 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4261 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4321 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4381 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4441 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4501 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4561 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4621 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4681 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4741 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4801 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4861 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4921 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4981 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5041 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5101 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5161 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5221 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5281 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5341 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5401 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5461 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5521 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5581 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5641 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5701 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5761 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5821 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5881 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5941 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6001 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6061 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6121 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6181 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6241 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6301 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6361 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6421 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6481 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6541 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6601 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6661 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6721 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6781 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6841 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6901 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6961 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7021 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7081 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7141 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7201 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7261 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7321 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7381 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7441 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7501 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7561 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7621 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7681 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7741 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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